Neha Parmar

Background Despite known clinical risk factors, predicting anthracycline cardiotoxicity remains challenging. Objectives This study sought to develop a clinical and genetic risk prediction model for anthracycline cardiotoxicity in childhood cancer survivors. Methods We performed exome sequencing in 289 childhood cancer survivors at least 3 years from anthracycline exposure. In a nested case-control design, 183 case patients with reduced left ventricular ejection fraction despite low-dose doxorubicin (≤250 mg/m2), and 106 control patients with preserved left ventricular ejection fraction despite doxorubicin >250 mg/m2 were selected as extreme phenotypes. Rare/low-frequency variants were collapsed to identify genes differentially enriched for variants between case patients and control patients. The expression levels of 5 top-ranked genes were evaluated in human induced pluripotent stem cell–derived cardiomyocytes, and variant enrichment was confirmed in a replication cohort. Using random forest, a risk prediction model that included genetic and clinical predictors was developed. Results Thirty-one genes were differentially enriched for variants between case patients and control patients (p < 0.001). Only 42.6% case patients harbored a variant in these genes compared to 89.6% control patients (odds ratio: 0.09; 95% confidence interval: 0.04 to 0.17; p = 3.98 × 10–15). A risk prediction model for cardiotoxicity that included clinical and genetic factors had a higher prediction accuracy and lower misclassification rate compared to the clinical-only model. In vitro inhibition of gene-associated pathways (PI3KR2, ZNF827) provided protection from cardiotoxicity in cardiomyocytes. Conclusions Our study identified variants in cardiac injury pathway genes that protect against cardiotoxicity and informed the development of a prediction model for delayed anthracycline cardiotoxicity, and it also provided new targets in autophagy genes for the development of cardio-protective drugs. (Preventing Cardiac Sequelae in Pediatric Cancer Survivors [PCS2]; NCT01805778)

Microscopy methods have provided most of our knowledge to date on the structural organisation of chromosomes. Even after decades of research, the high order structure of human chromosomes is still under investigation. The new generation of X-ray sources, X-ray Free Electron Lasers (XFELs) have opened an opportunity for imaging these complex structures with higher than optical resolution but without the radiation damage that usually accompanies X-ray imaging. Here we report our first experimental steps towards imaging micron-sized human chromosomes using the SACLA XFEL facility in Japan using the MAXIC chamber. The paper highlights the sample preparation of chromosomes, staining and drying conditions used, as well as the imaging optimisations and shows our fi rst results.

Gokshura, a drug of herbal origin, is being used extensively by Ayurvedic physicians for the management of various disease conditions like Mutrakrichchhra(dysuria), Prameha(Diabetes), Kasa(Cough), Shwasa(Asthma), Hridroga(Cardiac disorders). It is one of the components of Dashamoolawhich is popularly used for its anti-inflammatory action. Although official source of Gokshura is authenticated as Tribulus terrestris as per API; it’s some synonyms like Shwadanshtra, Trikantakaetc. do not match the morphological characters of Tribulus terrestris. In this article, an attempt is made to enlist and discuss all the source plants which come under the controversial aspect of Gokshura.

Encryption is a technique which is used to encode a file which can only accessible by authorised person. In this paper, an audio file is encrypted by applying transformation and cryptography. Transformation is used to convert an audio file from time domain to frequency domain. In this we can apply encryption on only lower frequency band. So transformation provides lower frequency band. Cryptography is the study of information hiding. For transformation FFT (Fast Fourier Transformation) is used. For encryption RSA is used. After applying the encryption on different frequency bands, we observe that, the encryption on the lower frequency band is more effective than the higher one. So, we would apply encryption on lower frequencies. Keywords— FFT, Cryptography, Encryption, Transformation Technique.

Introduction : Acharya Susruta has described it under the heading of ashta maha gada- under the big umbrella of eight difficult to treat disorders and it is defined as violent as an enemy. Aim and objectives: The study was planned to compare the clinical efficacy of Kasisadi taila (Group I) with the Jatyadi taila (Group II) in the management of arsha with respect to haemorrhoids. Observation :28 patients with classical sign and symptoms of Arsha (haemorrhoids) visiting O.P.D. & I.P.D. of Shalya Tanta ; department of I.P.G.T. & R.A., Hospital, Jamnagar were selected for the present study. In the Kasisadi taila group, two patients got marked improvement and five patients got moderate improvement. In Jatyadi taila group, three patients got moderate improvement and three patients got improvement. Result is depending upon time factor which is related to chronicity and severity of the disease. Conclusion : Kasisadi Taila as local application with routine standard treatment is more effecti...

FFT is a transformation technique. It is used to transform an audio signal from time domain to frequency domain. It can also apply with Watermarking and Encryption techniques. Watermarking is a technique to hide any kind of data in another file where as Encryption is a technique to change the data an unreadable form. After applying FFT we get the effective output.

Bilva (Aegle marmelos Corr.) is a very well-known and extensively used medicinal plant since ancient times. It is also very important plant for Hindus as it is used in rituals and considered as an auspicious tree. It is one of the ingredients of the groups, Bruhat panchamoola and Dashamoola, which are indicated in various diseased conditions. In different conditions various parts of Bilva are being used medicinally i.e Patra, (leaves) Moola (root) and Apakva phala (unripe fruit) & pakva phala (ripe fruit). In the present study, 32 Ayurvedic texts were referred to collect references of Bilva being used in the form of Pathyakalpana. From these compendia 142 Kalpanas were found, maximum number of Kalpanas found were in Ashtanga samgraha (35), followed by Ashtangahrudya (33), Vangasena (31), Charaka samhita (27) etc. Dosage forms mostly used were Yavagu (rice gruel) (31), followed by Peya (thin gruel) (29), Ksheerapaka (medicated milk) (28), Yusha (Soup from pulses) (25), etc. Kalpanas ...

Rationale & Objective The concept of Prakriti is unique to Ayurveda, which is used for deciding the preventive and curative strategy to be adopted in the treatment of patients. It is the total of anatomical, physiological, and psychological domains of an individual. The diseases often manifest by susceptibility that depends upon Prakriti of individuals. COVID 19 is a new disease, where the status of the susceptibility of its victim in terms of Prakriti is not known. This study has been undertaken to determine the Prakriti of COVID 19 positive patients. Method The validated instrument CCRAS Prakriti assessment scale has been applied to the COVID 19 positive patients admitted between 16 May 2020 to 10 June 2020 at COVID hospital. Result Data of 117 patients aged 10 to 80 years have been analyzed. The ratio of male-female patients was 1.8:1. Most patients belonged to Vata-KaphaPrakriti (27%).Individuals with their Prakriti found in order of frequency were Pitta-Kapha (21%), Kapha (20%)...

Objective : The aims of our prospective observational study were to evaluate the (1) reliability of clinical signs in the early detection of diaphragm palsy (DP); (2) reliability of ultrasonography using echo machine as a bedside tool for the diagnosis of DP; and (3) does early diaphragm plication result in the improved outcome? We also sought to determine the incidence and predominant risk factors for DP and diaphragm plication at our center. Materials and Methods : This prospective observational study included patients with suspected DP from January 2015 to December 2018. Patients with suspected DP were initially evaluated by bedside ultrasonography using echo machine and confirmed by fluoroscopy. Diaphragm plication was considered for patients having respiratory distress, difficult weaning, or failed extubation attempt without any obvious cardiac or pulmonary etiology. Patients were followed for 3 months after discharge to assess diaphragm function. Results: A total of 87 patient...

Gokshura a well-known drug in Ayurveda which is extensively used in many disease conditions like dysuria, asthma, diabetes, cough, oedema, cardiac disorders etc. Tribulus terrestris (Family – Zygophyllaceae) is an official source of Gokshura as per API. Five species of genus Tribulus are found throughout India with a slight morphological difference. In this study, three different species of Tribulus genus from different regions were subjected for molecular characterization by RAPD method. Analysis showed that three different samples gave clearly similar banding pattern with each of the random primers used and 80% similarity between the three samples were observed when the results were subjected to band scoring and analysis with clustering. Even through the micromorpholgical observations showed differentiating characters in mature carpels and intrastaminal glands of the selected species.

The present work aim was “Formulation Strategy for Dissolution Enhancement of Simvastatin”. Simvastatin is lipid lowering drug which is known as HMG CoA reductase. The objective of this study was to increase the solubility of poorly water soluble drug, namely simvastatin, by the formation of solid dispersion and complex and also using the microwave induction technique on these formations. For solid dispersion method dispersion carrier used were poloxamer 407 and gelucire 44/14. The fusion method was used to prepare the dispersions. For inclusion complexation method β-cyclodextrin derivative of cyclodextrin was used to prepare complex with drug. Kneading method was used for formulation. After completion of these two techniques these polymers were used for the microwave induced fusion method. All the ratio of drug and polymer were used to heat for different time interval. These samples were used for solubility measurement. In the solid dispersion technique, simvastatin show higher inc...

The present work was based on "To Formulate, evaluate and optimization of Matrix Tablets of Lornoxicam by using Hydrophilic and Hydrophobic Polymers for marketing benefits and having same dissolution profiles." Drug lornoxicam has a selective analgesic and non-steroidal anti-inflammatory effect. This once a daily formulation of Lornoxicam comprises at one extended release (SR) component comprising a release controlling material (HPMC-K100M and EUDRAGIT RS 100). Preformulation study was carried out for measurement of drug solubility, particle size, and micrometric property and drug-excipient compatibility study. Sustain release part was prepared by wet granulation method. In vitro drug release profile for optimized formulation was at least 99 % after 24 hours. Batch 30 selected as the optimize batch and check the reproducibility for this batch as batch no. 35. In vitro release profiles of all the batches were performed with the kinetic model studies. Response surface graph ...

Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simple reverse filtration step. Fluorescence microscopy was used to validate the chromosome suspension after each filtration step. This reverse filtration technique is an improvement in both procedure time and chromosome recovery compared to currently used post-purification methods. Chromosomes purified by our method could be used for many applications, such as structural studies using microfluidics and high resolution imaging or generation of chromosome paints and sequencing after flow cytometry.