Reza Mohammadi

In present study, the molecular diversity of twenty five durum wheat genotypes including 21 breeding lines and 3l and races were evaluated using 11inter simple sequence repeat sprimers. The used primers generated 108 fragments, among which 83 bands (about 77%) were polymorphic. A total of 83 polymorphic bands were scored with average 7.54 polymorphic fragments per primer. The average of polymorphism information content index was 0.31, indicating the efficiency of the markers in discrimination of the populations. Cluster analysis based on UPGMA algorithm and Dice similarity coefficient classified the 25 genotypes into four separated groups. The result of principal coordinate analysis (PCoA) was in congruity with cluster analysis.

The present study was conducted to evaluate the genetic diversity within a set of 16 advanced lines of durum wheat (Triticum turgidum) using 9 ISSR primers. A total of 73 polymorphic fragments were scored with average 8.1 polymorphic bands per primer. Cluster analysis using UPGMA method and Dice similarity coefficient categorized the genotypes into 4 separated groups. Estimates of genetic similarity ranged from 0.34 to 0.86 with an average of 0.68. The frequency distribution of genetic distance between pairs of accessions based on marker data indicates a normal distribution. The results showed a considerable polymorphism among durum advanced lines, reflecting the efficiency of ISSRs in the assessment of the genetic diversity.

Durum wheat (Triticum turgidum var. durum) is considered as an important food crop. The most important challenge in production of most of the crops including durum wheat is drought stress. To evaluate drought tolerance of 25 durum wheat genotypes using polyethylene glycol (PEG) 6000, a factorial experiment based on completely randomized design with three replications was carried out in the tissue culture laboratory of islamic Azad University, Kermanshah, Iran in 2010-11. The results of ANOVA indicated significant differences between genotypes for traits Diameter of callus (DC) and Callus Growth Rate (CGR) under non-stress condition and for the traits relative growth rate (RGR), Relative fresh weight growth (RFWG), based on fresh weight, Callus Growth Rate (CGR), callus water content (CWC), Relative Water Content (RWC), fresh weight and dry weight is a significant difference (P<0.01) under drought stress. Cluster analysis based on the studied traits, classified the genotypes into four groups. The principal component analysis (PCA) and biplot technique classified the breeding lines 4, 11, 3 and 2 in same group as drought tolerant genotypes. The results verified a remarkable variation for callus induction ability in genetic materials under drought stress condition that can be used in durum wheat breeding program.

Preliminary results on detection of neurotransmitter serotonin using an electrochemical sensor, based on a composite made of core-shell molecularly imprinted polymer (MIP) nanoparticles and polyethylenedioxythiophene (PEDOT) film are described. MIP is grown on pre-formed SiO2 nanobeads by surface imprinting via reversible addition-fragmentation chain-transfer (RAFT) polymerization. The core-shell Ser-MIP@SiO2 nanobeads are integrated onto electrode surfaces to develop an electrochemical sensors for analyte serotonin. The entrapment of core-shell Ser-MIP@SiO2 nanobeads by the electropolymerization of PEDOT in an upside-down electrochemical cell is performed and preliminary results in sensing application are presented.

The combined effects of probiotic sequential inoculation (before or after fermentation), type of native Bifidobacterium strain (Bifidobacterium animalis spp. lactis PTCC 1631 and Bifidobacterium bifidum PTCC 1644) and final pH of fermentation (4.5 or 4.2) on ...

Burns are a major factor in injury mortality. The aim of this study was to explore the possible causes of fatal burns using Haddon's Matrix. This is a qualitative study using a phenomenological approach. We collected elicitation interview data using nine corroborators who were the most knowledgeable about the index burn event. Immediately after recording, the data was verbatim. Each event was analyzed using Haddon's Matrix. Interviewees provided detailed information about 11 burn cases. Overall, 202 burn-related factors were extracted. Using Haddon's Matrix, 43 risk factors were identified. The most common included the lack of basic knowledge of burn care, the use of unsafe appliances including kerosene heaters and stoves in hazardous environments such kitchens and bathrooms, poor burn care delivery system in hospitals, poor and unsafe living conditions, financial issues, and other factors detailed in the article. Our findings suggest burn related prevention efforts shou...

Integrating yield and stability of genotypes tested in unpredictable environments is a common breeding objective. The main goals of this research were to identify superior durum wheat genotypes for the rainfed areas of Iran and to determine the existence of different mega-environments in the growing areas of Iran by testing 20 genotypes in 4 locations for 3 years via GGE (genotype + genotype-by-environment) biplot analysis. Stability of performance was assessed by the Kang’s yield-stability statistic (YSi) and 2 new methods of yield-regression statistic (Ybi) and yield-distance statistic (Ydi).The combined analysis of variance showed that environments were the most important source of yield variability, and accounted for 76% of total variation. The magnitude of the GE interaction was ~10 times the magnitude of the G effect. The GGE biplot suggested the existence of 2 durum wheat mega-environments in Iran. The first mega-environment consisted of environments corresponding to ‘cold’ l...

The additive main effect and multiplicative interaction (AMMI) model and the phenotypic stability parameters, ecovalence (W2), regression coefficient (b), coefficient of determination (R2), coefficient of variation (CV), stability variance (S2), AMMI stability value (ASV), and TOP (proportion of environments in which a genotype ranked in the top third), were used to evaluate simultaneously the yield performance and stability of 17 spring safflower genotypes and to evaluate 26 rainfed environments during 2003–05 in Iran. These parameters were designated as Type-A and Type-B for genotypes and environments, respectively. Among Type-B parameters, Spearman’s rank correlation showed that the AMMI stability value (ASVj), ecovalence (Wj2), genotypic variance (Sj2), and coefficient of variation (CVj) were significantly and positively associated (P < 0.01), indicating that one of these parameters can be used as an alternative to the others, but were significantly and negatively correlated ...