The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after a conserved stem-loop sequence. The cleavage reaction requires at least two trans -acting factors: the stem-loop binding protein (SLBP),... more
The 3′ end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after a conserved stem-loop sequence. The cleavage reaction requires at least two trans -acting factors: the stem-loop binding protein (SLBP), which binds the stem-loop sequence, and the U7 snRNP that interacts with a sequence downstream from the cleavage site. Removal of SLBP from a nuclear extract abolishes 3′-end processing, and the addition of recombinant SLBP restores processing activity of the depleted extract. To determine the regions of human SLBP necessary for 3′ processing, various deletion mutants of the protein were tested for their ability to complement the SLBP-depleted extract. The entire N-terminal domain and the majority of the C-terminal domain of human SLBP are dispensable for processing. The minimal protein that efficiently supports cleavage of histone pre-mRNA consists of 93 amino acids containing the 73-amino-acid RNA-binding domain and 20 amino acids located immediate...
Research Interests: M Rna Processing, Biology, Medicine, Gene expression, Biological Sciences, and 15 moreMolecular and cellular biology, Humans, Mutation, Mice, Animals, Post-Transcriptional Gene Regulation, Histones, RNA-binding proteins, Ribonucleoproteins, Histone, Recombinant Proteins, Protein Binding, Nucleic Acid Conformation, binding sites, and Medical and Health Sciences
Translationally inactive histone mRNA is stored in frog oocytes, and translation is activated at oocyte maturation. The replication-dependent histone mRNAs are not polyadenylated and end in a conserved stem-loop structure. There are two... more
Translationally inactive histone mRNA is stored in frog oocytes, and translation is activated at oocyte maturation. The replication-dependent histone mRNAs are not polyadenylated and end in a conserved stem-loop structure. There are two proteins (SLBPs) which bind the 3′ end of histone mRNA in frog oocytes. SLBP1 participates in pre-mRNA processing in the nucleus. SLBP2 is oocyte specific, is present in the cytoplasm, and does not support pre-mRNA processing in vivo or in vitro. The stored histone mRNA is bound to SLBP2. As oocytes mature, SLBP2 is degraded and a larger fraction of the histone mRNA is bound to SLBP1. The mechanism of activation of translation of histone mRNAs may involve exchange of SLBPs associated with the 3′ end of histone mRNA.
Research Interests: Cellular Biology, Biology, Cell Biology, Medicine, Oogenesis, and 14 moreBiological Sciences, Xenopus, Molecular and cellular biology, Animals, Histones, RNA-binding proteins, Amino Acid Sequence, Base Sequence, Histone, Oocytes, Protein Biosynthesis, Molecular Sequence Data, binding sites, and Medical and Health Sciences
Translationally inactive histone mRNA is stored in frog oocytes, and translation is activated at oocyte maturation. The replication-dependent histone mRNAs are not polyadenylated and end in a conserved stem- loop structure. There are two... more
Translationally inactive histone mRNA is stored in frog oocytes, and translation is activated at oocyte maturation. The replication-dependent histone mRNAs are not polyadenylated and end in a conserved stem- loop structure. There are two proteins (SLBPs) which bind the 3* end of histone mRNA in frog oocytes. SLBP1 participates in pre-mRNA processing in the nucleus. SLBP2 is oocyte specific, is
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The 3* end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after a conserved stem-loop sequence. The cleavage reaction requires at least two trans-acting factors: the stem-loop binding protein (SLBP),... more
The 3* end of histone mRNA is formed by an endonucleolytic cleavage of the primary transcript after a conserved stem-loop sequence. The cleavage reaction requires at least two trans-acting factors: the stem-loop binding protein (SLBP), which binds the stem-loop sequence, and the U7 snRNP that interacts with a sequence downstream from the cleavage site. Removal of SLBP from a nuclear
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Replication-dependent histone mRNAs are the only metazoan mRNAs that are not polyadenylated, ending instead in a conserved stem–loop sequence. Histone pre-mRNAs lack introns and are processed in the nucleus by a single cleavage step,... more
Replication-dependent histone mRNAs are the only metazoan mRNAs that are not polyadenylated, ending instead in a conserved stem–loop sequence. Histone pre-mRNAs lack introns and are processed in the nucleus by a single cleavage step, which produces the mature 3′ end of the mRNA. We have systematically examined the requirements for the nuclear export of a mouse histone mRNA using the Xenopus oocyte system. Histone mRNAs were efficiently exported when injected as mature mRNAs, demonstrating that the process of 3′ end cleavage is not required for export factor binding. Export also does not depend on the stem–loop binding protein (SLBP) since mutations of the stem–loop that prevent SLBP binding and competition with a stem–loop RNA did not affect export. Only the length of the region upstream of the stem–loop, but not its sequence, was important for efficient export. Histone mRNA export was blocked by competition with constitutive transport element (CTE) RNA, indicating that the mRNA exp...
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Metazoan replication-dependent histone mRNAs end in a stem-loop sequence. The one known exception is the histone mRNA in amphibian oocytes, which has a short oligo(A) tail attached to the stem-loop sequence. Amphibian oocytes also contain... more
Metazoan replication-dependent histone mRNAs end in a stem-loop sequence. The one known exception is the histone mRNA in amphibian oocytes, which has a short oligo(A) tail attached to the stem-loop sequence. Amphibian oocytes also contain two proteins that bind the 3′ end of histone mRNA: xSLBP1, the homologue of the mammalian SLBP, and xSLBP2, which is present only in oocytes. xSLBP2 is an inhibitor of histone mRNA translation, while xSLBP1 activates translation. The short A tail on histone mRNAs appears at stage II to III of oogenesis and is present on histone mRNAs throughout the rest of oogenesis. At oocyte maturation, the oligo(A) tail is removed and the xSLBP2 is degraded, resulting in the activation of translation of histone mRNA. Both SLBPs bind to the stem-loop with the oligo(A) tail with similar affinities. Reporter mRNAs ending in the stem-loop with or without the oligo(A) tail are translated equally well in a reticulocyte lysate, and their translation is stimulated by th...
Research Interests: Molecular Biology, RNA, Biology, Medicine, Oogenesis, and 15 moreBiological Sciences, Molecular and cellular biology, Female, Animals, Genes, Histones, Reporter, Base Sequence, Histone, Oocytes, Nucleic Acid Conformation, Messenger, Protein Biosynthesis, Medical and Health Sciences, and In Vitro Techniques
The stem–loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3′-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To... more
The stem–loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3′-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 inXenopusoocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.
Research Interests: Biology, Medicine, Biological Sciences, Xenopus, Humans, and 15 moreAnimals, Genes, Molecular cloning, Histones, Zinc Finger, RNA-binding proteins, HeLa cells, Amino Acid Sequence, Base Sequence, Histone, DNA binding proteins, Zinc Finger Protein, Molecular Sequence Data, Psychology and Cognitive Sciences, and Medical and Health Sciences
There is a growing need to implement and evaluate the technological solutions that allow the early detection of age-related frailty and enable assessment of the predictive values of frailty components. The broad use of these solutions may... more
There is a growing need to implement and evaluate the technological solutions that allow the early detection of age-related frailty and enable assessment of the predictive values of frailty components. The broad use of these solutions may ensure an efficient and sustainable response of health and social care systems to the challenges related to demographic aging. In this paper, we present the protocol of the ModulEn study that aims to develop and validate a predictive model for frailty. For this purpose, the sample composed by older adults aged 65-80 years and recruited from the community will be invited to use an electronic device ACM Kronowise® 2.0. This device allows proactive and continuous monitoring of circadian health, physical activity, and sleep and eating habits. It will be used during a period of seven to ten days. The participants will also be given the questionnaires evaluating the variables of interest, including frailty level, as well as their experience and satisfact...
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AIMS AND OBJECTIVES This study aimed to analyse the prevalence and factors associated with suicidal ideation among family caregivers of people with mental disorders. BACKGROUND Studies conducted with family caregivers of people with... more
AIMS AND OBJECTIVES This study aimed to analyse the prevalence and factors associated with suicidal ideation among family caregivers of people with mental disorders. BACKGROUND Studies conducted with family caregivers of people with dementia and cancer point out a high prevalence of suicidal ideation among these subjects; however, this aspect has not yet been investigated among family caregivers of people with mental disorders. DESIGN This is a cross-sectional study, conducted with 537 family caregivers of patients from 16 Psychosocial Care Centers (CAPS) of the 21st Health Region of the state of Rio Grande do Sul, Brazil. METHODS Question 17 of the Self-Reporting Questionnaire (SRQ-20) was used for suicidal ideation screening. The prevalence of suicidal ideation was calculated according to sociodemographic and care variables, with confidence interval estimate (95% CI). Crude and adjusted odds ratios were calculated by logistic regression. The Guidelines to Reporting of Observational Studies in Epidemiology (STROBE Statement) was adhered in this study (See File S1). RESULTS The prevalence of suicidal ideation found in this study for the 30 days preceding the interview was 12.5% (95% CI: 10-15). The factors associated with the outcome were lower age, lower schooling, feeling of burden, self-report of stress problem and dissatisfaction with family relationships. CONCLUSION The prevalence of suicidal ideation among the studied family caregivers was high and strongly associated with issues regarding care, showing the need for interventions that provide support. RELEVANCE FOR CLINICAL PRACTICE Nurses are a large part of the workforce of the community mental health services. The careful characterisation of the subjects who show suicidal ideation, as performed in this study, may reveal specificities capable of refining the diagnostic potential for establishment of action plans in a timely manner, avoiding possible attempts or even the consummation of suicide.
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In the context of the long-term care for older adults, informal carers play a key role. Daily competing priorities or a care-skills deficit may lead them to stress, anxiety and/or depression. The iCarer project (AAL-2012-5-239) proposes... more
In the context of the long-term care for older adults, informal carers play a key role. Daily competing priorities or a care-skills deficit may lead them to stress, anxiety and/or depression. The iCarer project (AAL-2012-5-239) proposes the design and implementation of a cloud-inspired personalised and adaptive platform which will offer support to informal carers of older adults with cognitive impairment. By means of a holistic approach comprising technologies and services addressing the intelligent and interactive monitoring of activities, knowledge management for personalised guidance and orientation, virtual interaction, e-learning, care coordination facilities and social network services, iCarer aims to reduce the informal carer stress and to enhance the quality of care they provide, thus improving their quality of life. The iCarer platform will be evaluated through a multi-centre non-controlled study (4 months; 48 homes located in England and in Slovenia). Currently the iCarer ...
Research Interests: Psychology, Computer Science, Telemedicine, Medicine, Cloud Computing, and 13 moreAnxiety, Software, Library and Information Studies, Humans, Europe, Caregivers, Computer User Interface Design, Public health systems and services research, ANXIETY, Assisted Living, Home Care Services, Cognition disorders, and Health services for the aged
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Ricotta is a soft cheese that has a high moisture content and an initial pH above 6.0 and, thus, has a limited shelf-life even under refrigeration. The objectives of the present work were to calculate sensory and microbiological... more
Ricotta is a soft cheese that has a high moisture content and an initial pH above 6.0 and, thus, has a limited shelf-life even under refrigeration. The objectives of the present work were to calculate sensory and microbiological shelf-life using Weibull's distribution and to ...
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Research Interests: Endocrinology, Biology, Fertility, Lactation, Medicine, and 15 moreSignal Transduction, Theriogenology, Biological Sciences, Internal Medicine, Blood Glucose, Female, Animals, Cattle, Ovarian Follicle, Butyric Acid, Cellular Microenvironment, Follicular Fluid, Follicular phase, Medical and Health Sciences, and follicle
Research Interests: Psychology, Clinical Psychology, Psychometrics, Colombia, Medicine, and 15 moreFactor analysis, Humans, Female, Confirmatory factor analysis, Male, Confidence intervals, Concordance, Public health systems and services research, Analysis of Variance, Neoplasms, Cancer Patient, Correlation coefficient, Quality Life, Factor structure, and Psychological Stress
Research Interests: Geography, Demography, Epidemiology, Colombia, Data Analysis, and 15 moreAdolescent, HUMAN PAPILLOMAVIRUS, African American, Genetic Testing, Female, Coinfection, Cervical Cancer, Aged, Adult, Ethnic Group, Genetic Susceptibility, Cervical Intraepithelial Neoplasia, HPV Infection, Expected Value, and Alphapapillomavirus
Research Interests: Genetics, Cognitive Science, Biology, Immunohistochemistry, Cell Biology, and 14 moreMedicine, Drosophila, Animals, Neuronal Plasticity, Embryonic Development, SYNAPSES, RNA-binding proteins, Neuron, Base Sequence, Neuromuscular Junction, Neurosciences, Postsynaptic Density, Glutamate Receptor, and Molecular Sequence Data
Research Interests: Immune response, Biology, Inflammation, Motor neuron, Macrophages, and 15 moreMedicine, Nature, Amyotrophic Lateral Sclerosis, Biological Sciences, Humans, Mice, Female, Animals, Male, Mouse Model, Immune system, Monoclonal Antibody, Human Disease, Gene expression profiling, and Medical and Health Sciences
Metazoan replication-dependent histone mRNAs end in a conserved stem-loop rather than in the poly(A) tail found on all other mRNAs. The 3′ end of histone mRNA binds a single class of proteins, the stem-loop binding proteins (SLBP). In... more
Metazoan replication-dependent histone mRNAs end in a conserved stem-loop rather than in the poly(A) tail found on all other mRNAs. The 3′ end of histone mRNA binds a single class of proteins, the stem-loop binding proteins (SLBP). In Xenopus, there are two SLBPs: xSLBP1, the homologue of the mammalian SLBP, which is required for processing of histone pre-mRNA, and xSLBP2, which is expressed only during oogenesis and is bound to the stored histone mRNA in Xenopus oocytes. The stem-loop is required for efficient translation of histone mRNAs and substitutes for the poly(A) tail, which is required for efficient translation of other eucaryotic mRNAs. When a rabbit reticulocyte lysate is programmed with uncapped luciferase mRNA ending in the histone stem-loop, there is a three- to sixfold increase in translation in the presence of xSLBP1 while xSLBP2 has no effect on translation. Neither SLBP affected the translation of a luciferase mRNA ending in a mutant stem-loop that does not bind SL...
Research Interests: Molecular Biology, Biology, Cell Biology, Medicine, Biological Sciences, and 15 moreXenopus, In Vitro, Molecular and cellular biology, Humans, Female, Animals, In Vivo, Histones, RNA-binding proteins, Histone, Fusion Protein, Binding Site, Protein Biosynthesis, binding sites, and Medical and Health Sciences
Resumen Objetivos:  Cuantificar la prevalencia de ideación suicida en una población de estudiantes universitarios, caracterizar grupos en relación con la ideación y las conductas suicidas y determinar las variables que se asocian con... more
Resumen Objetivos:  Cuantificar la prevalencia de ideación suicida en una población de estudiantes universitarios, caracterizar grupos en relación con la ideación y las conductas suicidas y determinar las variables que se asocian con ideación suicida e intentos de ...
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Research Interests: Kinetics, Biology, Immunohistochemistry, Medicine, Angiogenesis, and 15 moreCell Division, Keratinocytes, Mice, Female, Animals, Hyperplasia, Fibroblast Growth Factor, CD, Clinical Sciences, Endothelial cell, INVESTIGATIVE, Knockout Mice, Blood Vessel, Interferon Alpha, and Basic Fibroblast Growth Factor
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Research Interests: Biology, Cell Adhesion, Metastasis, Medicine, In Situ Hybridization, and 15 moreHumans, Differentiation, Base Sequence, Cadherin, Collagenase, Gastric Carcinoma, Biochemistry and cell biology, Matrix Metalloproteinase, Early Gastric Cancer, Molecular Sequence Data, Cadherins, lymph node metastasis, Stomach neoplasms, advanced gastric cancer, and Paediatrics and reproductive medicine
Tenemos conciencia de que ha finalizado el proceso de exploración de nuestro planeta y de que una misma dinámica económica nos engloba a todos y nos uniformiza culturalmente. Pero nos damos cuenta también de que se desarrollan entre... more
Tenemos conciencia de que ha finalizado el proceso de exploración de nuestro planeta y de que una misma dinámica económica nos engloba a todos y nos uniformiza culturalmente. Pero nos damos cuenta también de que se desarrollan entre nosotros ...
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Información del libro La Fe pública judicial y las funciones del secretario en la nueva ley orgánica del poder judicial: con formularios e indicación de la resoluciÓn procedente en los procedimientos civiles y penales.
... DETERMINADAS CARACTERÍSTICAS: modelo vitamínico de Warr Eg compensación ... para el interfaz individuo-organización Papel protagonista de las organizaciones en la ... Harrison (1978), ajuste persona y ambiente en aspectos objetivos y... more
... DETERMINADAS CARACTERÍSTICAS: modelo vitamínico de Warr Eg compensación ... para el interfaz individuo-organización Papel protagonista de las organizaciones en la ... Harrison (1978), ajuste persona y ambiente en aspectos objetivos y subjetivos, mecanismos de ...
