Stefania Masci
Università degli Studi della Tuscia, DAFNE, Faculty Member
Research Interests: Biology and Ranunculaceae
... IN THE WHEAT ENDOSPERM AND BELONG TO THE GLUTENIN FRACTION P. Ferrante, C. Patacchini, S. Masci, R. D'Ovidio, D. Lafiandra ... This latter was perfomed by the Molecular Structure Facility at University of... more
... IN THE WHEAT ENDOSPERM AND BELONG TO THE GLUTENIN FRACTION P. Ferrante, C. Patacchini, S. Masci, R. D'Ovidio, D. Lafiandra ... This latter was perfomed by the Molecular Structure Facility at University of California, Davis by means of an Applied Biosystems Procise ...
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... The uee of biotechnology to study wheat endosperm development and improve grain quality. D. Lafiandra, S. Masci and R. D'Ovidio Copyright Year:2004 ISBN: 978-1-84755-209-9 DOI:10.1039/9781847552099-00001. ...
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Research Interests: Medicine, Food Allergy, Allergy, Gliadin, Globulin, and 3 moreGluten, Immunoglobulin E, and Prolamin
... Bread Wheat Line Over-Expressing a LMW-GS. S. Masci, R. D'Ovidio, F. Scossa, C. Patacchini, D. Lafiandra, OD Anderson and AE Blechl Copyright Year:2004 ISBN: 978-1-84755-209-9 DOI:10.1039/9781847552099-00010. ...
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Research Interests: Engineering, Chemistry, Food Science, Polymorphism, Seed germination, and 14 moreBiological Sciences, Gliadin, Endosperm, Gluten, Protein Complex Detection, Seed storage, Embryos, Glutenin, Cereal Science, Wheat flour, Low molecular weight, Seedling Growth, High Molecular Weight, and Genetic control
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Research Interests: Chemistry, Food Science, Medicine, Analysis, Gluten, and 7 moreFermentation, Fermented food, DDC, LC MS, Hydrolysis, Competitive ELISA, and Prolamin
Research Interests: Chemistry, Food Science, Starch, Medicine, Foods, and 3 moreFerulic Acid, Syringic Acid, and Phenolic Acid
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The genes encoding the HMW glutenin subunits belong to a small gene family where not all members are expressed in cultivated wheat varieties. In particular, the Ay HMW glutenin subunit is always absent. On the basis of nucleotide sequence... more
The genes encoding the HMW glutenin subunits belong to a small gene family where not all members are expressed in cultivated wheat varieties. In particular, the Ay HMW glutenin subunit is always absent. On the basis of nucleotide sequence comparisons of the ...
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Micronutrient malnutrition affects more than half of the world population. Reduced bioavailability of microelements in the raw materials is considered one of the main causes of mineral deficiency in populations whose diet is largely based... more
Micronutrient malnutrition affects more than half of the world population. Reduced bioavailability of microelements in the raw materials is considered one of the main causes of mineral deficiency in populations whose diet is largely based on the consumption of staple crops. In this context, the production of low phytic acid (lpa) cereals is a main goal of the breeding programs, as phytic acid (PA) binds essential mineral cations such as iron (Fe), zinc (Zn), manganese (Mn), potassium (K), calcium (Ca) and magnesium (Mg) precipitating in the form of phytate salts poorly digested by monogastric animals, including humans, due to the lack of phytases in the digestive tract. Since PA limits the bioavailability of microelements, it is widely recognized as an anti-nutritional compound. A Targeting Induced Local Lesions IN Genomes (TILLING) approach has been undertaken to silence the genes encoding the TdABCC13 proteins, known as Multidrug-Resistance associated Proteins 3 (TdMRP3), transpor...
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... Language, English. Notes, references. ... C-terminal and internal sequences of a low molecular weight (LMW-s) type of glutenin subunit by Vensel, WH (USDA, ARS, Western Regional Research Center, Albany, CA.); Tarr, GE; Kasarda, DD... more
... Language, English. Notes, references. ... C-terminal and internal sequences of a low molecular weight (LMW-s) type of glutenin subunit by Vensel, WH (USDA, ARS, Western Regional Research Center, Albany, CA.); Tarr, GE; Kasarda, DD (Jul-Aug 1995) in English. ...
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Research Interests: Biology, Proteomics, Medicine, Biological Sciences, Globulin, and 13 moreHeat stress, Seeds, Durum Wheat, Proteome analysis, Physiological Stress Markers, Two-Dimensional Gel Electrophoresis, Triticum, Two dimensional Gel Electrophoresis, Proteome, PLANT PROTEINS, Glutenin, Stress Physiological, and Medical and Health Sciences
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Research Interests: Biochemistry, Chemistry, Mass Spectrometry, Medicine, Gene expression, and 15 moreBiological Sciences, Gliadin, Escherichia coli, Gluten, Flour, High Pressure Liquid Chromatography, Inclusion Bodies, Molecular cloning, Cysteine, Molecular weight, Amino Acid Sequence, Heterologous Expression, Glutenin, Molecular Sequence Data, and Medical and Health Sciences
Low molecular weight glutenin subunits (LMW-GS) are typically subdivided into three groups, according to their molecular weights and isoelectric points, namely the B-, C-, and D groups. Enriched B- and C-type LMW-GS fractions extracted... more
Low molecular weight glutenin subunits (LMW-GS) are typically subdivided into three groups, according to their molecular weights and isoelectric points, namely the B-, C-, and D groups. Enriched B- and C-type LMW-GS fractions extracted from the bread wheat cultivar Chinese Spring were characterized using high performance liquid chromatography (HPLC) directly interfaced with electrospray ionization mass spectrometry and HPLC coupled off-line with matrix-assisted laser desorption/ionization mass spectrometry, in order to ascertain the number and relative molecular masses of the components present in each fraction and determine the number of cysteine residues. About 70 components were detected in each of the fractions examined by the combined use of these two techniques, with 18 components common to both fractions. Analysis of the fractions after alkylation with 4-vinylpyridine allowed determination of the number of the cysteines present in about 40 subunits. The proteins detected were tentatively classified based on the relative molecular masses and number of cysteine residues. Cross-contamination was found in both B- and C- fractions, along with the presence of D-type LMW-GS. The two fractions also contained unexpected components, probably lipid transfer proteins and omega-gliadins. The presence of extensive microheterogeneity was suggested by the detection of several co-eluting proteins with minor differences in their molecular masses.