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Habeeb Al-Solami
    Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and... more
    Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%) ،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in the Kingdom of Saudi Arabia, these were Chrysomya albiceps; Ch. marginalis; Sarcophaga dux and Wohlfahrtia bella. The appearance of the W. bella species was first recorded in Saudi Arabia. The results showed the presence of forensic insects most of the time during the year, and there were significant differences in different months of the year, and the reason for this may be due to differences in climatic conditions, temperature, and relative humidity which are considered among the most important factors affecting the reproduction and spread of the insects.
    Abstract Objectives Plant extracts have been a safe and eco-friendly substituent of chemical pesticides, used against mosquitoes to prevent vector-borne infections e.g., chikungunya, yellow fever, dengue, filariasis, dirofilariasis... more
    Abstract Objectives Plant extracts have been a safe and eco-friendly substituent of chemical pesticides, used against mosquitoes to prevent vector-borne infections e.g., chikungunya, yellow fever, dengue, filariasis, dirofilariasis tularemia, malaria, and many other diseases. Methods The larvicidal activities of the acetone extracts of four plants e.g., Lantana camara, Ruta chalepensis, Rhazya stricta, and Acalypha fruticosa was evaluated against Culex pipiens. The composition secondary metabolites of the most effective extract (L. camara) was examined using gas chromatography-mass spectrometry (GC-MS). The expression level of Acetylcholinesterase (AChE) and Glutathione S-transferase (GST) genes were assessed through qRT-PCR. Results The results revealed that the extract of L. camara caused 98% mortality in Cx. pipiens followed by R. stricta (91%), A. fruticosa (79%), and R. chalepensis (69%) as compared to azadirachtin, used as a positive control. The results showed that LD50 and LD90 of the extract of L. camara were significantly higher as compared to R. stricta, A. fruticosa, and R. chalepensis extract. The extract of L. camara also significantly reduced the activities of AChE and GST as compared to the larvae, treated with the extracts of another plant extracts as well as a positive control. The chemical composition of L. camara, was determined through GC-MS. Conclusions The most important insecticidal compounds were including undecane, terephthalic acid, dimethyl-propane-thiosulfinate, fluorobenzoic acid octadecenoic acid. The insecticidal activity of the L. camara extracts against Cx. pipiens might be due to one or more of these compounds.
    Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and... more
    Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%)،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in th...
    Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and... more
    Most of the murders are shrouded in mystery and the absence of a lot of forensic evidence that helps the authorities achieve justice and reveal the culprit. The insects present at the crime scene and their various stages remain clues and evidence that are very important in knowing and revealing the circumstances of the incident. Therefore, determining the species of insect related to forensic medicine is very important for each region separately. The present study was planned to survey of forensic insects species and its seasonal activity in Makkah governorate. The local white rabbits were used as a mammal closer to the human corpse. The work was carried out of one year from January 2007 to December 2019. During the survey, 15,757 flies of importance forensic insects were collected which represent three families: Muscidae (47.1%) ،Calliphoridae (30.0%); Sarcophagidae (22.6%), and Muscidae (47.1%). In this study, four flies species were reported for the first time in Makkah City in t...
    Nanotechnology is a newer branch of material science that thrives in the interdisciplinary fields including biomedical, therapeutics, diagnostics etc. Among the metallic nanoparticles, gold nanoparticles (AuNPs) in particular are known... more
    Nanotechnology is a newer branch of material science that thrives in the interdisciplinary fields including biomedical, therapeutics, diagnostics etc. Among the metallic nanoparticles, gold nanoparticles (AuNPs) in particular are known for their versatile biological applications in several scientific fields in general and specifically the biomedical field. The current article accounts for an environmentally safe method for AuNPs synthesis using the chloroauric acid and ethanolic leaf extract of Nicotiana glauca (NG), for the first time. Various analytical techniques identified properties of synthesized AuNP to include UV-Vis absorption spectroscopy, Fourier-Transform Infrared Radiation (FT-IR), and scanning electron microscopy (SEM) for surface morphology. The average diameter of synthesized AuNP was about 100 nm. Antimicrobial activity of AuNPs was investigated on Grampositive ( Staphylococcus aureus ), Gram-negative (Escherichia coli , Proteus mirabilis, and Shigella flexneri) and...
    Each year millions of peoples die to due mosquito's related diseases. Resistance of these mosquitoes towards chemical pesticides is one of serious issue. Bio-control is one of the safe ways to control these vectors. The aim of our... more
    Each year millions of peoples die to due mosquito's related diseases. Resistance of these mosquitoes towards chemical pesticides is one of serious issue. Bio-control is one of the safe ways to control these vectors. The aim of our study was to evaluate three different plant extract against the 4 instar larvae of Culex quinquefasciatus mosquitoes. Two ethanolic plants extract and one plant essential oil (EOs) was used for the larvicidal activity ranging from concentration of 0 to 900 ppm against the 4 instar larvae of Culex quinquefasciatus. To determine the LC50 and LC90 value, data was subjected to probit analysis. Mosquito larvae's used during this study was identified using COI gene sequencing. Among them Argania spinosa oil showed moderate larvicidal effect at low concentration but Thevetia peruviana extract and Saueda monoica extract shows high larvicidal activity at low concentration respectively. The LC50 and LC90 values for Thevetia peruviana extract was 105.95 and 2...
    Each year millions of peoples die to due mosquito's related diseases. Resistance of these mosquitoes towards chemical pesticides is one of serious issue. Bio-control is one of the safe ways to control these vectors. The aim of our... more
    Each year millions of peoples die to due mosquito's related diseases. Resistance of these mosquitoes towards chemical pesticides is one of serious issue. Bio-control is one of the safe ways to control these vectors. The aim of our study was to evaluate three different plant extract against the 4 instar larvae of Culex quinquefasciatus mosquitoes. Two ethanolic plants extract and one plant essential oil (EOs) was used for the larvicidal activity ranging from concentration of 0 to 900 ppm against the 4 instar larvae of Culex quinquefasciatus. To determine the LC50 and LC90 value, data was subjected to probit analysis. Mosquito larvae's used during this study was identified using COI gene sequencing. Among them Argania spinosa oil showed moderate larvicidal effect at low concentration but Thevetia peruviana extract and Saueda monoica extract shows high larvicidal activity at low concentration respectively. The LC50 and LC90 values for Thevetia peruviana extract was 105.95 and 2...
    Three sustained tablet formulas of the bacterial insecticides Spinosad and VectoBac as well as the insect growth regulator Dudim were evaluated for larvicidal activity against larval stages and pupae until adult emergence of Culex... more
    Three sustained tablet formulas of the bacterial insecticides Spinosad and VectoBac as well as the insect growth regulator Dudim were evaluated for larvicidal activity against larval stages and pupae until adult emergence of Culex pipiens. Effective control with 90-100% inhibition of adult emergence (% IE) was used as a criterion to evaluate the effectiveness of the test formulations. A high level of residual efficacy with 90-100% IE was achieved for 8 and 11 weeks post-treatment by using tablet formulations of Spinosad and Dudim, respectively. On the other hand, larval treatments with VectoBac tablet formulations showed ineffective control with 63% IE for the 1 week post-treatment and after that it began to give continuous excellent effective control (90-100% IE) for 5 successive weeks. These estimated times indicate that Dudim formulation proved to be more effective against C. pipiens larvae than formulations of Spinosad and VectoBac by about 1.4 and 2.2 times, respectively. Apart...
    Laboratory trials were conducted to determine the larvicidal activity of the bacterial insecticides (spinosad and vectobac), the insect growth regulators, IGRs (sumilarv and dudim) and the plant extracts of neem (Azadirachta indica) and... more
    Laboratory trials were conducted to determine the larvicidal activity of the bacterial insecticides (spinosad and vectobac), the insect growth regulators, IGRs (sumilarv and dudim) and the plant extracts of neem (Azadirachta indica) and usher (Calotropis procera) against Aedes aegypti (L.). The toxicity curves showed that the bioinsecticide spinosad proved to be more effective than vectobac against A. aegypti larvae by about 11.1 times. Such a fact was highly pronounced on the basis of LC50 values (concentration which to kill 50% of larvae) which were 0.009 ppm and 0.1 ppm, respectively. According to IC50 values (concentration which to inhibit the emergence of 50 % of adults), the results indicated that mosquito larvae of A. aegypti were more susceptible to the IGR dudim (0.00038 ppm) than sumilarv (0.004 ppm) by about 10.5 folds. The plant extract of neem was also more effective against the present larvae than usher extract, with IC50 of 35 ppm and 66 ppm, respectively. Taking coef...
    In this study for the first-time gold nanoparticles (AuNPs) were synthesized (MiAuNPs) using Melilotus indicus L. extract (MiExt) by eco-friendly method for different bioactivity applications. Various analytical techniques used for... more
    In this study for the first-time gold nanoparticles (AuNPs) were synthesized (MiAuNPs) using Melilotus indicus L. extract (MiExt) by eco-friendly method for different bioactivity applications. Various analytical techniques used for identifying properties of MiAuNPs include powder X-ray diffraction, UV-Vis absorption spectroscopy, Fourier-Transform Infrared Radiation (FT-IR), and scanning electron microscopy (SEM). The average diameter of MiAuNPs was about 8 nm (crystallite size by XRD was estimated around 13 nm). Antimicrobial activities MiAuNPs revealed significant effect against Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Proteus mirabilis, and Shigella flexneri) and fungi (Candida albicans). The anticancer activity which may be found in the MiExt as well as extract with MiAuNPs was checked using HepG2 and HeLa cell lines. Results revealed that the MiExt alone was stimulatory to Hela cells growth, while MiAuNPs inhibit it. Both MiExt and MiAuNPs also in...
    ABSTRACTThis article reports on the silver nanoparticles (AaAgNPs) that were green-synthesized by using Artemisia annua L. extract (AaExt) and their collective biological applications. Active biomolecules in the extract and extract... more
    ABSTRACTThis article reports on the silver nanoparticles (AaAgNPs) that were green-synthesized by using Artemisia annua L. extract (AaExt) and their collective biological applications. Active biomolecules in the extract and extract containing AgNPs were characterized using Fourier-transform-infrared-spectroscopy (FTIR) and AgNPs were monitored by UV/vis spectroscopy and SEM (scanning electron microscopy) analysis. The size of the particle is around 100 nm. The antibacterial activity was measured by the disk diffusion method against the Gram-negative/positive pathogenic bacteria. The extract and extract containing AgNPs showed a significant antibacterial activity. Cytotoxic potential of the synthesized AgNPs was analyzed against the rat splenocytes. The results showed that there were cytotoxic effects of A. annua leaves extract but stimulatory effects when the extract contained AgNPs on normal splenocytes. Extract of A. annua showed very little increase in liver enzymes. Regarding th...
    Nanotechnology is a newer branch of material science that thrives in the interdisciplinary fields including biomedical, therapeutics, diagnostics etc. Among the metallic nanoparticles, gold nanopar-ticles (AuNPs) in particular are known... more
    Nanotechnology is a newer branch of material science that thrives in the interdisciplinary fields including biomedical, therapeutics, diagnostics etc. Among the metallic nanoparticles, gold nanopar-ticles (AuNPs) in particular are known for their versatile biological applications in several scientific fields in general and specifically the biomedical field. The current article accounts for an environmentally safe method for AuNPs synthesis using the chloroauric acid and ethanolic leaf extract of Nicotiana glauca (NG), for the first time. Various analytical techniques identified properties of synthesized AuNP to include UV-Vis absorption spectroscopy, Fourier-Transform Infrared Radiation (FT-IR), and scanning electron microscopy (SEM) for surface morphology. The average diameter of synthesized AuNP was about 100 nm. Antimicrobial activity of AuNPs was investigated on Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Proteus mirabilis, and Shigella flexneri) and ...
    The main goal of this study was the monitoring of the population fluctuation of the prevalent dipterous fly species complex and to generate a baseline data in Jeddah Governorate and it’s surroundings using malaise and yellow sticky traps... more
    The main goal of this study was the monitoring of the population fluctuation of the prevalent dipterous fly species complex and to generate a baseline data in Jeddah Governorate and it’s surroundings using malaise and yellow sticky traps in animal pens including sheep, cow, cattle and vegetable market. Data recovered indicated the continued presence of four prevalent fly species including the tachinid species complex, the house fly Musca domestica L., the flesh fly Sarcophaga carnaria and the hover fly (Sphaerophoria) at the rate of 59.91%, 23.55%, 16.14% and 0.41% respectively. It is noteworthy to report the continued presence throughout the year of the tachinid species complex which might indicate it’s efficiency in suppressing the fly population, the fluctuation of other prevalent species that are present extantly.
    The main goal of this study was the monitoring of the population fluctuation of the prevalent dipterous fly species complex and to generate a baseline data in Jeddah Governorate and it’s surroundings using malaise and yellow sticky traps... more
    The main goal of this study was the monitoring of the population fluctuation of the prevalent dipterous fly species complex and to generate a baseline data in Jeddah Governorate and it’s surroundings using malaise and yellow sticky traps in animal pens including sheep, cow, cattle and vegetable market. Data recovered indicated the continued presence of four prevalent fly species including the tachinid species complex, the house fly Musca domestica L., the flesh fly Sarcophaga carnaria and the hover fly (Sphaerophoria) at the rate of 59.91%, 23.55%, 16.14% and 0.41% respectively. It is noteworthy to report the continued presence throughout the year of the tachinid species complex which might indicate it’s efficiency in suppressing the fly population, the fluctuation of other prevalent species that are present extantly.