Tsenka R. Tsanova

Artefacts made from stones, bones and teeth are fundamental to our understanding of human subsistence strategies, behaviour and culture in the Pleistocene. Although these resources are plentiful, it is impossible to associate artefacts to specific human individuals1 who can be morphologically or genetically characterized, unless they are found within burials, which are rare in this time period. Thus, our ability to discern the societal roles of Pleistocene individuals based on their biological sex or genetic ancestry is limited2–5. Here we report the development of a non-destructive method for the gradual release of DNA trapped in ancient bone and tooth artefacts. Application of the method to an Upper Palaeolithic deer tooth pendant from Denisova Cave, Russia, resulted in the recovery of ancient human and deer mitochondrial genomes, which allowed us to estimate the age of the pendant at approximately 19,000–25,000 years. Nuclear DNA analysis identifies the presumed maker or wearer of the pendant as a female individual with strong genetic affinities to a group of Ancient North Eurasian individuals who lived around the same time but were previously found only further east in Siberia. Our work redefines how cultural and genetic records can be linked in prehistoric archaeology.

The behavioral dynamics underlying the expansion of Homo sapiens into Europe remains a crucial topic in human evolution. Owing to poor bone preservation, past studies have strongly focused on the Initial Upper Paleolithic (IUP) stone tool record. Recent excavations and extensive radiocarbon dating at Bacho Kiro Cave (Bulgaria) pushed back the arrival of IUP H. sapiens into Europe to ca. 45,000 years ago. This site has exceptional bone preservation, and we present the study of 7431 faunal remains from across two IUP layers (I and J) and one Middle Paleolithic layer (K). We identified a shift in site use and occupation intensity through time, marked by increased find density and human modifications in Layer I. Alongside a decrease in carnivore presence and seasonality data demonstrating human presence in all seasons, this indicates a more frequent or prolonged occupation of the site by IUP groups. Contrarily, the dietary focus across the IUP and Middle Paleolithic layers is similar, centered on the exploitation of species from a range of habitats including Bos/Bison, Cervidae, Equidae, and Caprinae. While body parts of large herbivores were selectively transported into the site, the bear remains suggest that these animals died in the cave itself. A distinct aspect of the IUP occupation is an increase in carnivore remains with human modifications, including these cave bears but also smaller taxa (e.g., Canis lupus, Vulpes vulpes). This can be correlated with their exploitation for pendants, and potentially for skins and furs. At a broader scale, we identified similarities in subsistence behavior across IUP sites in Europe and western Asia. It appears that the first IUP occupations were less intense with find densities and human modifications increasing in succeeding IUP layers. Moreover, the exploitation of small game appears to be limited across IUP sites, while carnivore exploitation seems a recurrent strategy.

Modern humans appeared in Europe by at least 45,000 years ago1–5, but the extent of their interactions with Neanderthals, who disappeared by about 40,000 years ago6, and their relationship to the broader expansion of modern humans outside Africa are poorly understood. Here we present genome-wide data from three individuals dated to between 45,930 and 42,580 years ago from Bacho Kiro Cave, Bulgaria1,2. They are the earliest Late Pleistocene modern humans known to have been recovered in Europe so far, and were found in association with an Initial Upper Palaeolithic artefact assemblage. Unlike two previously studied individuals of similar ages from Romania7 and Siberia8 who did not contribute detectably to later populations, these individuals are more closely related to present-day and ancient populations in East Asia and the Americas than to later west Eurasian populations. This indicates that they belonged to a modern human migration into Europe that was not previously known from the...

... 5 : Laboratoire des sciences du climat et de l'environnement (LSCE). CNRS : UMR1572 – INSU – CEA : DSM/LSCE – Université de Versailles-Saint Quentin en Yvelines – Institut Pierre Simon Laplace. 6 : Geophysical institute. Bulgarian Academy of Sciences. ...

Bone surface modifications are crucial for understanding human subsistence and dietary behaviour, and can inform about the techniques employed in the production and use of bone tools. Permission to destructively sample such unique artefacts is not always granted. The recent development of non-destructive proteomic extraction techniques has provided some alternatives for the analysis of rare and culturally significant artefacts, including bone tools and personal ornaments. The Eraser Extraction Method (EEM), first developed for ZooMS analysis of parchment, has recently been applied to bone and ivory specimens. To test the potential impact of the EEM on ancient bone surfaces, we analyse six anthropogenically modified Palaeolithic bone specimens from Bacho Kiro Cave (Bulgaria) through a controlled sampling experiment using qualitative and 3D quantitative microscopy. Although the overall bone topography is generally preserved, our findings demonstrate a slight flattening of the microtop...

Abstract This paper reports the preliminary results of two test pit excavations in Toplitsa cave in Northern Bulgaria and discusses the potential for future investigations of the Pleistocene layers associated with Marine Isotope Stage 3 and the transitional Middle to Upper Palaeolithic period. We describe the lithostratigraphic layers, archaeological finds and features attributable to the Upper Palaeolithic. The preliminary chrono-cultural interpretation of the early Upper Palaeolithic geoarchaeological deposit at Toplitsa cave is based on the identification of Campanian Ignimbrite/Y-5 (CI) tephra, radiocarbon dates and the technological assessment of a small lithic artefact assemblage in comparison with the regional Initial Upper Palaeolithic (IUP) technologies. Toplitsa cave, located in the Eastern Balkans (Northern Bulgaria), was excavated in 2011 with the main goal of investigating deposits encompassing the transition from the Middle to Upper Palaeolithic. Two test pits (TP) were excavated: TP I, incorporating four lithostratigraphic layers, was located in the cave interior and TP II, encompassing six lithostratigraphic layers, was established closer to the cave entrance. Both TPs were generally poor in archaeological remains which impacted the chrono-cultural attribution of the Pleistocene layers. Moreover, in some spots Holocene faunal remains likely intruded into older layers. In consequence, the preliminary chronological investigation has been focused on applying both tephrochronology and radiocarbon dating of the early Upper Palaeolithic deposits. We present a preliminary assessment of the stratigraphy and report on the identification of large amounts of glass shards belonging to the CI eruption (ca. 39,000 years old (ka BP)) in TP II Layers 2 and 3, below which there is a well preserved hearth. This fire-pit carved in Layer 5 (TP II) and underlying the Y-5 tephra is the most significant find. Two Ursus spelaeus teeth from the underlying Layer 5 have been radiocarbon dated to 49,770–45,750 calibrated years before present (cal BP; 95.4% probability) and to 48,210–45,050 cal BP (95.4% probability). The lithics found in Layer 5 are technologically consistent with the Initial Upper Palaeolithic (IUP) or possibly with other transitional Middle to Upper Palaeolithic assemblages. Layer 5 from TP II in Toplitsa overlaps chronologically with other regional records. These include the final Middle Palaeolithic with leaf points from Musselievo (estimated to ca. 50–45 ka cal BP), the transitional Middle to Upper Palaeolithic assemblage from Temnata Cave Layer VI (trench II, which is covered by CI tephra and has been dated to older than 39 ka BP), the earliest laminar Upper Palaeolithic (i.e. IUP) from Temnata Cave Layer 4 (trench I, dating from 47.6 to 40.5 ka cal BP), the IUP from Bacho Kiro Cave Layers J and I (dating to 46.0–43.3 ka cal BP) and Kozarnika cave Layer 6/7 (dated to ca. 49.1–44.0 ka cal BP).

The transition from the Middle Stone Age (MSA) to the Later Stone Age (LSA) in South Africa was not associated with the appearance of anatomically modern humans and the extinction of Neandertals, as in the Middle to Upper Paleolithic transition in Western Europe. It has therefore attracted less attention, yet it provides insights into patterns of technological evolution not associated with a new hominin. Data from Border Cave (KwaZulu-Natal) show a strong pattern of technological change at approximately 44–42 ka cal BP, marked by adoption of techniques and materials that were present but scarcely used in the previous MSA, and some novelties. The agent of change was neither a revolution nor the advent of a new species of human. Although most evident in personal ornaments and symbolic markings, the change from one way of living to another was not restricted to aesthetics. Our analysis shows that: ( i ) at Border Cave two assemblages, dated to 45–49 and >49 ka, show a gradual abando...

Southwest Asia is a key region in current debates surrounding the appearance of the first cultures attributed to anatomically modern humans, particularly the Aurignacian and preceding cultural units of the Iranian Zagros, Levant, and the Balkans (Baradostian, Ahmarien, Kozarnikien, etc.). The Zagros mountain range encompasses an immense territory that remains understudied with regard to the Upper Paleolithic as well as the first bladelet industries traditionally presumed to be the work of anatomically modern humans. Concerning the emergence of the Aurignacian, the sites of Warwasi rockshelter and Yafteh cave in the central Zagros are considered to show evidence of in situ evolution of the Upper Paleolithic from the local Mousterian. This hypothesis is tested by way of a taphonomic, techno-typological and economic approach applied to the Upper Paleolithic levels of Warwasi (spits LL-AA) and Yafteh (the series from the lower part of the sequence). A comparison of the techno-economic features of both assemblages demonstrates a conceptual bond with contemporaneous techno-complexes from Levant and Europe (Ahmarian, Protoaurignacian, etc.). The techno-typological Middle Paleolithic character of the Warwasi lithic assemblage permits a discussion of a possible in situ dependence/continuum from the Mousterian or perhaps particular activities linked to the type of the occupation of the site. However, bladelet technology cannot be considered as rooted in the Zagros Mousterian. Consequently the origin of the Aurignacian sensu stricto has to be reconsidered.

L'industrie lithique de la couche 11 de Bacho Kiro est connue pour avoir été interprétée comme une origine possible de l'Aurignacien, sur la base d'une analyse essentiellement typologique de l'outillage. Ce travail présente les principaux résultats d'une étude techno-...

The discovery of a Neandertal skeleton in the archaeological sequence of Saint-Césaire plays a central role in the debates about the Middle to Upper Paleolithic transition. However, a variety of reasons contributed to prevent universal acceptance of this finding. Specifically, doubts ...