The effect produced on Vero cell monolayers by toxins derived from Staphylococcus strains was characterized. 210 milk samples taken from dairy cows suffering from sub-clinical mastitis were analyzed. Strains belonging to the... more
The effect produced on Vero cell monolayers by toxins derived from Staphylococcus strains was characterized. 210 milk samples taken from dairy cows suffering from sub-clinical mastitis were analyzed. Strains belonging to the Staphylococcus genus were isolated from 73 of these milk samples. The production of toxins was then stimulated from these strains when they were cultured in Dolman's medium. The study of cell cultures showed that 53 toxin samples induced marked and irreversible cellular changes. This is compared to 42 samples (57.5%) which were strongly cytotoxic. The remaining 11 samples were shown to be slowly cytotoxic. 16% of the total toxins did not induce cell damage and 11% of the toxins produced cellular damage that was reversible in less than 24 hrs, and were designated as cytotonic. Haemolytic actively in vitro, using sheep red blood cells, was assessed using toxins that caused alteration in the monolayers. The results indicate that 46.51% of the toxins showed beta...
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Résumé/Abstract Hydrodistilled oils ofBaccharis spartioides from three populations of Argentina (Northwest, Central and Patagonia areas) were analyzed by using GC and GC/MS. Thirty-nine compounds were identified in the oils and a... more
Résumé/Abstract Hydrodistilled oils ofBaccharis spartioides from three populations of Argentina (Northwest, Central and Patagonia areas) were analyzed by using GC and GC/MS. Thirty-nine compounds were identified in the oils and a relatively high variation in ...
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The chloroform extract of Larrea divaricata was evaluated against Macrophomina phaseolina and the possible allelopathic effect action in tomato (Licopersicon esculentum Mill.). The antifungal activity in vitro, expressed in percentage of... more
The chloroform extract of Larrea divaricata was evaluated against Macrophomina phaseolina and the possible allelopathic effect action in tomato (Licopersicon esculentum Mill.). The antifungal activity in vitro, expressed in percentage of inhibition of the mycelia growth was of 91,37%, to a concentration of extract of 1000 mg/ml. The percentage of germination and the height of the plants determined to the 21 days post-germination demonstrate that the chloroform extract of L. divaricata, at concentrations of 500 and 1000 mg/ml, does not present negative alellopathic effect.
The antifungal activity of the chloroform extract (EC) of Minthostachys verticillata (Lamiaceae) was evaluated on the growth of phytopathogenic fungus of Fusarium, through dilution method in agar. To a 1000 mg/ml concentration, the EC... more
The antifungal activity of the chloroform extract (EC) of Minthostachys verticillata (Lamiaceae) was evaluated on the growth of phytopathogenic fungus of Fusarium, through dilution method in agar. To a 1000 mg/ml concentration, the EC inhibited the growth of Fusarium solani in 16,5%, Fusarium graminearum in 65,9% and Fusarium verticillioide in 70,7% of inhibition being this last species the one that showed greater susceptibility the extract.
Mitogenic and antigenic lymphocyte stimulation was examined in pigs that had been immunized with 2 inactivated vaccines which had been prepared with Aujesky's disease virus (ADV), strain RC/79. One vaccine was partially purified by... more
Mitogenic and antigenic lymphocyte stimulation was examined in pigs that had been immunized with 2 inactivated vaccines which had been prepared with Aujesky's disease virus (ADV), strain RC/79. One vaccine was partially purified by ultra-centrifugation (Vaccine A) the other one was not (Vaccine B). A second dose of vaccine had no influence over the blastogenic response when the lymphocytes were stimulated with phytohemoagglutinin (PHA). Lymphocyte response to the ADV antigen in the immunized pigs was significantly higher at day 30 post inoculation than at day 0 indicating that it was highly specific. Cellular antigens contained in the viral cultures produced a slight non-specific response as shown by a low increase in the levels of lymphocyte blastic transformation (LBT) in the control group at day 30 p.i., this group only received a non infected Vero cell suspension. This was the case in pigs that received vaccine A as well as in those that were vaccinated with vaccine B. Vacci...
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The PHA and ConA mitogens are able to stimulate to CD3+ T cells included (¿/¿ ¿/¿) CD4+ and CD8+ T cells leading to its polyclonal activation. Minthostachys verticillata (Griseb.) Epling commonly known as "peperina" is an... more
The PHA and ConA mitogens are able to stimulate to CD3+ T cells included (¿/¿ ¿/¿) CD4+ and CD8+ T cells leading to its polyclonal activation. Minthostachys verticillata (Griseb.) Epling commonly known as "peperina" is an aromatic bush that grow in the north-west and central regions of Argentina. Its essential oil has demonstrated anti-allergic and lymphoproliferative properties in vitro. In this work, the in vitro effects of pure compounds isolated of the essential oil of M verticillata: pulegone, menthone and limonene, like polyclonals activators on human lymphocytes, were investigated. The cells, isolated from peripheral blood, were cultured in independent assay and stimulated with PHA, ConA, pulegone, menthone or limonene. In addition co-stimulated cultures with: pulegone/men-thone, pulegone/limonene, menthone/limonene and pulegone/menthone/limonene in per-centtage similar to which is these compounds in the vegetable (63% of pulegone, 16% of menthone and 2% of limonene...
The antiviral activity of alcoholic extracts of several species belonging to the Asteraceae, Labiatae, Plantaginaceae, Schizaceae, Umbelliferae, Usneaceae and Verbenaceae families has been studied. The tests were carried out in Vero... more
The antiviral activity of alcoholic extracts of several species belonging to the Asteraceae, Labiatae, Plantaginaceae, Schizaceae, Umbelliferae, Usneaceae and Verbenaceae families has been studied. The tests were carried out in Vero celís-pseudorabies virus strain RC/79 (herpes suis virus) system. Eight plant extracts (Achyrocline satureioides, Ambrossia tenuifolia, Baccharis articulata, Eupatorium buniifolium, Mynthostachys verticillata, Plantago brasiliensis, Plantago mayor L and Verbascum thapsus) were able to inhibit at least 2 log, the viral infectivity.
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In a previous work, we have demonstrated that Minthostachys verticillata essential oil has immunomodulatory effects in vitro on cells from allergic patients. Here we characterized main components of M. verticillata essential oil and also... more
In a previous work, we have demonstrated that Minthostachys verticillata essential oil has immunomodulatory effects in vitro on cells from allergic patients. Here we characterized main components of M. verticillata essential oil and also tested if these compounds modulate In vitro and in vivo the immediate-type allergic reaction. Gas chromatography was used to identify main components of the essential oil. Pulegone (63.4 %), menthone (15.9 %), and limonene (2.1 %) were found as main classes. IL-13 levels were evaluated from lymphocytes cultures stimulated with allergen alone or combined with monoterpenes. All compounds stimulated cell proliferation but, interestingly, promoted a reduction of IL-13 values, limonene and the mixture of the three compounds being the most active. β-Hexosaminidase release was determined from basophils to which essential oil or monoterpenes were added. We observed that, whichever combination of monoterpenes was used, β-hexosaminidase release was diminished...
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Baccharis articulata is native of América and traditionally used for the treatment of digestive disorders and urinary infections. Cytotoxicity of aqueous extracts of B. articulata was investigated in Vero cells. As the maximal non... more
Baccharis articulata is native of América and traditionally used for the treatment of digestive disorders and urinary infections. Cytotoxicity of aqueous extracts of B. articulata was investigated in Vero cells. As the maximal non cytotoxic concentration has been established, this concentration has been used to evaluate antiviral and virucidal activities against Herpes suis virus type 1, member of the same subfamily of Herpes simplex virus. Aqueous extracts of B. articulata exhibited more than 95% of virucidal activity. These findings support their potential application as a disinfectant or antiseptic with low toxicity and provide a valuable knowledge to ethnopharmacology properties of Baccharis articulata.
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Minthostachys verticillata (Lamiaceae), popularly known as peperina is largely used in popular medicine for its digestive, carminative, antispasmodic and antirheumatic properties. There are no reports of repeated exposure toxicity to... more
Minthostachys verticillata (Lamiaceae), popularly known as peperina is largely used in popular medicine for its digestive, carminative, antispasmodic and antirheumatic properties. There are no reports of repeated exposure toxicity to guarantee their safety. The present study investigated the chemical composition, analyzed by GC-FID, and the 90-day toxicity and genotoxicity effect of M. verticillata essential oil (Mv-EO), using Wistar rats as test animals. The rats were divided into four groups (5rats/sex/group) and Mv-EO was administered on diet at doses of 0, 1, 4 and 7g/kg feed. The main components of Mv-EO were pulegone (64.65%) and menthone (23.92%). There was no mortality, adverse effects on general conditions or changes in body weight, food consumption and feed conversion efficiency throughout the study in male and female rats. Subchronic administration of Mv-EO did not alter the weights, morphological and histopathological analyses of liver, kidney and intestine. Genotoxicity...
Research Interests: Lamiaceae, Kidney, Liver, Female, Animals, and 9 moreMale, Bone marrow, Comet Assay, Intestines, Wistar Rats, Monoterpenes, Weight Gain, Blood cells, and Menthol
... Laura N. Cariddi,* Adriana Panero, Mirta S. Demo, Liliana I. Sabini and Ana M. Maldonado Immunology Laboratory of the Microbiology and Immunology Department ... Louis, USA) 1 mmol/L, disolved in 0.1 mol/L of citrate buffer, pH 5. The... more
... Laura N. Cariddi,* Adriana Panero, Mirta S. Demo, Liliana I. Sabini and Ana M. Maldonado Immunology Laboratory of the Microbiology and Immunology Department ... Louis, USA) 1 mmol/L, disolved in 0.1 mol/L of citrate buffer, pH 5. The system was incubated to 37ºC for 1 h. ...
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ABSTRACT. There are no antigenic differences between known strains of Aujeszky's Disease virus or the Pseudorabies virus (PV). The characterization of these viruses has been based on pathological and/or pathogenic aspects of the... more
ABSTRACT. There are no antigenic differences between known strains of Aujeszky's Disease virus or the Pseudorabies virus (PV). The characterization of these viruses has been based on pathological and/or pathogenic aspects of the host. In this study, PV (RC/79 strain) ...