Spodoptera exigua

... R., Fuchs, T., Benedict, J., Parker, R., Sparks, S., Norman, J., Leser, J., Knutson, A., Minzenmayer, R. & Frisbie, R. (1996) Management guidelines for the beet armyworm on cotton. 11 pp. Texas A&M University. Texas Agriculture Extension Service Bulletin, April 1996. Idris, AB & ...

EFEKTIVITAS INSEKTISIDA NABATI DAUN KERSEN (Muntingia calabura L.), SERAI WANGI (Cymbopogon nardus L.) DAN INSEKTISIDA HAYATI BAKTERI Bacillus thuringiensis, JAMUR Beauveria bassiana DALAM PENGENDALIAN EKOLOGIS HAMA ULAT GRAYAK (Spodoptera exigua Hurbner) PADA TANAMAN BAWANG MERAH (Allium cepa var ascalonicum L.) VARIETAS BIMA BREBES

Beet armyworm, Spodoptera exigua (Hübner) is an agronomical important and most devastating polyphagous pest that damages a variety of crops around the globe including China. Quercetin is one of the abundant dietary flavonoids and the important defense allelochemicals in plants. Therefore, the changes in insect detoxi-fication enzymes activities in response to plants allelochemicals may result increased the sensitivity to insecticides. In this study, we examined the induced effect of quercetin on larval tolerance to lambda-cyhalothrin in S. exigua. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly synergized the lambda-cyhalothrin toxicity in quercetin-fed S. exigua larvae. Moreover, larval weight significantly reduced in quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin treatment. Furthermore, our results showed that the P450 detoxification enzyme effectively increased in all treatments as compared to the control. Quantitative Real-time PCR analysis revealed that expression level of CYP6AE10 significantly upreg-ulated in larvae treated with quercetin, lambda-cyhalothrin and quercetin + lambda-cyhalothrin in the midgut and fat body respectively. In addition, RNAi mediated knockdown of CYP6AE10 in S. exigua larvae significantly decreased the transcription level of target cytochrome P450 gene followed by the exposure with quercetin, lambda-cyhalothrin, and quercetin + lambda-cyhalothrin. Similarly, the knockdown of CYP6AE10 by the injection of dsRNA led to increased mortality after the treatment with respective chemicals. Overall, these data showed that P450s might possibly play an important role in the metabolic adaptation of S. exigua larvae to its host plant defense allelochemicals as well as insecticides. In conclusion, S. exigua can take benefit from its host plant's secondary metabolites to elaborate its defense against synthetic insecticides.

Induced plant responses to insect attack include the release of volatile chemicals. These volatiles are used as host-location signals by foraging parasitoids, which are natural enemies of insect herbivores. A plant's response to herbivory can be influenced by factors present in insect oral secretions. Volicitin (N-(17-hydroxylinolenoyl)-L-glutamine), identified in beet armyworm (Spodoptera exigua) oral secretions, stimulates volatile release in corn (Zea mays L.) seedlings in a manner similar to beet armyworm herbivory. Volicitin is hypothesized to trigger release of induced volatiles, at least in part, by modulating levels of the wound hormone, jasmonic acid (JA). We compare the sesquiterpene volatile release of damaged leaves treated with aqueous buffer only or with the same buffer containing volicitin or JA. Leaves were damaged by scratching with a razor and test solutions were applied to the scratched area. The leaves were either excised from the plant or left intact shortly after this treatment. Plants were treated at three different times (designated as Evening, Midnight, and Morning) and volatiles were collected in the subsequent photoperiod. JA and volicitin treatments stimulated the release of volatile sesquiterpenes, namely β-caryophyllene, (E)-α-bergamotene, and (E)-β-farnesene. In all cases, JA stimulated significant sesquiterpene release above mechanical damage alone. Volicitin induced an increase in sesquiterpene volatiles for all excised-leaf bioassays and the Midnight intact plants. Volicitin treatments in the Evening and Morning intact plants produced more sesquiterpenes than the untreated controls, while mechanical damage alone produced an intermediate response that did not differ from either treatment group. Excised leaves produced a 2.5- to 8.0-fold greater volatile response than similarly treated intact plants. Excision also altered the ratio of JA-and volicitin-induced sesquiterpene release by preferentially increasing (E)-β-farnesene levels relative to β-caryophyllene. The inducibility of volatile release varied with time of treatment. On average, sesquiterpene release was highest in the Midnight excised leaves and lowest in the Morning intact plants. The duration of induced volatile release also differed between treatments. On average, JA produced a sustained release of sesquiterpenes over time, with over 20% of the combined sesquiterpenes released in the third and final volatile collection period. In contrast, less than 8% of the combined sesquiterpenes induced by volicitin were emitted during this period. The large quantitative differences between intact plants and detached leaves suggest that the results of assays using excised tissues should be cautiously interpreted when considering intact-plant models.

Tachinid parasitoidsExorista xanthaspis (Wiedemann),Nemorilla maculosa (Meigen),Palesisa maculosa (Villeneuve) andDrino imberbis (Wiedemann) were obtained fromSpodoptera exigua (Hübner) (Noctuidae) larvae collected from cotton fields in Turkey.S. exigua is a new host record forN. maculosa, andP. maculosa is recorded from Turkey for the first time.

Arthropods
ISSN 2224-4255

Volume 6, Number 4, 1 December 2017
http://www.iaees.org/publications/journals/arthropods/articles/2017-6(4)/2017-6(4).asp

Cover Pages

[Front Pages (105K)] [Back Pages (55K)]
Articles

Sexual dimorphism and inter-individual variation in the rove beetle, Creophilus maxillosus L. (Col: Staphylinidae)
Arthropods, 2017, 6(4): 107-116
Mohammad Shahbaz, Gadir Nouri-Ganbalani
[Abstract] [XML] [EndNote] [RefManager] [BibTex] [DOAJ] [PubMed]
[ Full PDF (247K)]
[Email Article] [Comment/Review Article]



Temperature dependent development parameters and population life table of beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)
Arthropods, 2017, 6(4): 117-125
HonQing Dai, GuiLu Zhang, WenJun Zhang
[Abstract] [XML] [EndNote] [RefManager] [BibTex] [DOAJ] [PubMed]
[ Full PDF (201K)]
[Email Article] [Comment/Review Article]



Comparative management of Chilo suppressalis (Walker) (Lepidoptera: Crambidae) by convenient pesticides and non-chemical practices in a double rice cropping system
Arthropods, 2017, 6(4): 126-136
M. N. Poor Amiri, Faramarz Alinia, Sohrab Imani, et al.
[Abstract] [XML] [EndNote] [RefManager] [BibTex] [DOAJ] [PubMed]
[ Full PDF (235K)]
[Email Article] [Comment/Review Article]

A total of 189 nucleopolyhedrovirus (NPV) clones were obtained from the NPV-infected larvae of the common cutworm, Spodoptera litura, collected in Japan. Restriction endonuclease (REN) analysis demonstrated that these 189 S. litura NPV (SpltNPV) clones exhibited 33 distinct REN patterns that represented three distinct NPV types designated as type A, type B, and type C SpltNPVs based on the similarity of overall REN patterns. Comparative examination of REN patterns from these clones in this study with those from NPVs published previously indicated that type A SpltNPV corresponded to Spodoptera littoralis NPV (SlNPV)-D or SlNPV-B, whereas type B SpltNPV corresponded to the NPVs widely identified in S. litura larvae in Japan, China, and the Philippines. The overall REN pattern of type C SpltNPV did not resemble REN patterns from any of those NPVs previously identified in S. litura and S. littoralis and was distinct from the REN patterns of NPVs from Autographa californica, Bombyx mori, Hyphantria cunea, Spodoptera exigua, Lymantria dispar, and Orgyia pseudotsugata, suggesting that type C SpltNPV was a novel NPV pathogenic for S. litura. Comparative characterization with S. litura cultured cells and larvae showed that the biological properties with respect to productivity and virulence differed significantly not only among the types of SpltNPV but also among the variants within a SpltNPV type, demonstrating that SpltNPV-C3, a clone from type C SpltNPV, caused rapid mortality of S. litura larvae compared with those clones of type A or type B SpltNPV. These results indicate that the natural heterogeneity of baculoviruses can be exploited to develop NPV strains suitable for the control of S. litura and suggest that the novel SpltNPV-C3 isolated in this study has high potential to be developed as an effective pest control agent for S. litura.

The role of polyhedrin in the occlusion of virions was studied by substituting two heterologous polyhedrin-coding sequences, one from a multiple-nucleocapsid (M) nucleopolyhedrovirus (NPV) of Spodoptera exigua (Se) and one from a single-nucleocapsid (S) NPV of Buzura suppressaria (BusuNPV), into the genome of Autographa californica (Ac) MNPV. Both heterologous polyhedrin genes were highly expressed and polyhedra were produced in the nuclei of cells infected with the respective recombinant AcMNPVs. Polyhedra produced by the recombinant with BusuNPV polyhedrin showed normal occlusion of multiple-nucleocapsid virions and were equally as infectious to S. exigua larvae as were wild-type AcMNPV polyhedra. This indicates that virion occlusion is not specific with respect to whether the virions or polyhedrin are from an SNPV or MNPV. Polyhedra produced by the recombinant containing the SeMNPV polyhedrin had an altered morphology, being pyramidal rather than polyhedral in shape, and many few...

Previous studies revealed that chitinase could enhance the insecticidal activity of Bacillus thuringiensis and it has been used in combination with B. thuringiensis widely. However, the expression of B. thuringiensis chitinase is rather low and needs induction by chitin, which limits its field application. It would make sense to constitutively express the chitinase at a sufficiently high level to offer advantages in biological control of pests. In this study, a signal peptide-encoding sequence-deleted chitinase gene from B. thuringiensis strain 4.0718 under the control of dual overlapping promoters plus Shine–Dalgarno sequence and terminator sequence of cry1Ac3 gene was cloned into shuttle vector pHT315 and introduced into an acrystalliferous B. thuringiensis strain Cry−B. The recombinant plasmid was stably maintained over 240 generations in Cry−B. Chitinase was overexpressed within the sporangial mother cells in the form of spherical crystal-like inclusion bodies. The chitinase inclusions could be solubilized and exhibit chitinolytic activity in 30 mmol l−1 Na2CO3–0.2% β-mercaptoethanol buffer at a wide range of alkaline pH values, and what’s more, the chitinase inclusions potentiated the insecticidal effect of Cry1Ac protoxin when used against larvae of Spodoptera exigua and Helicoverpa armigera.

Host plants play an important role in population increases and outbreaks of cosmopolitan polyphagous insect pest, such as Spodoptera exigua (Hübner; Lepidoptera: Noctuidae). We investigated the impact of various host plants (cauliflower, peas, and wheat) on fitness and relative growth rate of S. exigua and intrinsic rate of population increase. The S. exigua population was collected from cauliflower fields from Multan, Pakistan. Comparison of various life-history traits on three different host plants revealed significant differences that might be correlated with the fitness costs. The mean number of eggs laid by female reared on cauliflower was significantly greater than on peas or wheat. Similarly, eggs viability, intrinsic rate of population increase, adult eclosion, and adult’s longevity were significantly higher on cauliflower than on peas and wheat. The low survival on peas and wheat suggest the unsuitability of these host plants, while the higher pupal weight on cauliflower suggest that it provides better food quality to S. exigua compared with peas and wheat. On peas and wheat, fitness declined with each generation suggesting that the average fitness of individuals may decline due to the elimination of insects with higher fitness. Our results suggest that cauliflower was the most nutritive and preferred host plant. Such results could be useful to design cultural management strategies, which may include trap cropping.

Jasmonic acid (JA) has long been hypothesized to be an important regulator of insect-induced volatile emission; however, current models are based primarily on circumstantial evidence derived from pharmacological studies. Using beet armyworm caterpillars (BAW: Spodoptera exigua) and intact corn seedlings, we examine this hypothesis by measuring both the time-course of insect-induced JA levels and the relationships between endogenous JA levels, ethylene, indole and sesquiterpenes. In separate Morning and Evening time-course trials, BAW feeding stimulated increases in JA levels within the first 4–6 h and resulted in maximal increases in JA, indole, sesquiterpenes and ethylene 8–16 h later. During BAW herbivory, increases in JA either paralleled or preceded the increases in indole, sesquiterpenes and ethylene in the Morning and Evening trials, respectively. By varying the intensity of the BAW herbivory, we demonstrate that strong positive relationships exist between the resulting variation in insect-induced JA levels and volatile emissions such as indole and the sesquiterpenes. To address potential signaling interactions between herbivore-induced JA and ethylene, plants were pretreated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception. 1-MCP pretreatment resulted in reduced production of ethylene and volatile emission following BAW herbivory but did not alter the insect-induced accumulation of JA. Our results strongly support a role for JA in the regulation of insect-induced volatile emission but also suggest that ethylene perception regulates the magnitude of volatile emission during herbivory.

A recombinant AcMNPV containing the green fluorescent protein (gfp) gene under the polyhedrin promoter (polh) was used to investigate the expression of the gfp gene as well as the production of recombinant extracellular virus in 14 continuous insect cell lines, including Heliothis virescens (BCIRL-HV-AM1), Helicoverpa zea (BCIRL-HZ-AM1), Anticarsia gemmatalis (BCIRL-AG-AM1), Trichoplusia ni (TN-CL1), Spodoptera frugiperda (IPLB-SF21), Spodoptera exigua (BCIRL/AMCY-Se-E1 and BCIRL/AMCY-Se-E5), Bombyx mori (BMN), Sf9 (a clone of IPLB-SF21), and five cell line clones of BCIRL-HV-AM1. The susceptibility of the cell lines to the recombinant virus (AcMNPV.GFP) was ascertained by calculating the mean percentage number of green light-emitting cells as well as by TCID50 titration of extracellular virus with fluorescence as a sign of infection. Of the 14 cell lines tested, all were permissive with varying degrees to Ac-MNPV.GFP, except BCIRL-HV-AMCL2 and BCIRL-HZ-AM1, both grown in serum-containing medium, and BMN, grown in serum-free medium, which were nonpermissive to the virus. Except for BCIRL/AMCY-Se-E1, IPLB-SF21, and four of the five BCIRL-HV-AM1 clones, all the other cell lines (BCIRL-HV-AM1, BCIRL-AG-AM1, TN-CL1, Se-E5, and Sf9) expressed detectable levels of GFP by 48 h postinoculation. The BCIRL/AMCY-Se-E1 and IPLB-SF21 cells, grown in serum-free medium (Ex-Cell 401), expressed detectable levels of GFP at 72 h postinoculation. By contrast, in BCIRL/AMCY-Se-E1 in serum-containing medium (Ex-Cell 401+10% FBS [fetal bovine serum]), GFP was detected at 48 h postinoculation. Furthermore, TN-CL1 cells produced the largest mean percentage number of fluorescent (76.6%) cells in both serum-containing and serum-free medium (64.8%) at 120 h postinoculation. All the BCIRL-HV-AM1 clones showed no GFP expression until 96 h postinoculation, and only then about 1% of the cell population fluoresced. The mean extracellular virus (ECV) production at 120 h postinoculation was highest in BCIRL/AMCY-Se-E5 cells grown in Ex-Cell 401+10% FBS (37.8×106 TCID50/ml) followed by BCIRL-HV-AM1 in TC199-MK (33.4×106 TCID50/ml). Only the BCIRL-HV-AMCL3 clone produced any substantial level of ECV at 120 h postinoculation (16.9×106 TCID50/ml). However, there was no significant correlation between ECV production and the mean percentage number of fluorescent cells. This study provides further information on the susceptibility of 14 insect cell lines to a recombinant AcMNPV containing the green fluorescent protein gene. This information might avail researchers with information to facilitate decisions as to what other cell lines are available for in vitro studies of the gfp gene.

The beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) is a highly polyphagous pest which causes considerable economic losses to cotton and many vegetable crops. Tannins are among the most important secondary metabolites in cotton plants. We show that tannic acid enhances the toxic effect of chlorantraniliprole on S. exigua when presented in combination. Bioassays using third-instar S. exigua larvae on an artificial diet showed that consumption of tannic acid with chlorantraniliprole at the concentration of (2 mg/g and LC50 0.018 mg/L) had higher toxicity when compared to either chlorantraniliprole or tannic acid alone (LC50 0.027 mg/L). The diet containing tannic acid with chlorantraniliprole significantly prolonged larval and pupal developmental time and extended mean generation time and total pre-oviposition period compared to either chemical alone. Moreover, fecundity, survival rate, reproductive value, intrinsic rate of increase, finite rate of increase and net reproduction rate declined significantly when exposed to the combined treatment. No difference was observed between tannic acid and the control. Meanwhile, tannic acid with chlorantraniliprole had markedly antifeedant effects; causing significant decline in the relative growth rate (RGR), the relative consumption rate (RCR), the efficiency of conversion of ingested food (ECI), the efficiency of conversion of digested food and an increase in the approximate digestibility (AD) compared to either chemical alone. Tannic acid with chlorantraniliprole also decreased the insect's carbohydrate, lipid and protein contents significantly. The results showed that the interaction between tannic acid and chlorantraniliprole on the growth inhibition of larvae was additive and tannic acid increased the toxicity of chlorantraniliprole to insects. The results of this study provide information useful in integrated pest management programs for S. exigua and show that tannic acid combined with chlorantraniliprole may be a route to reducing the use of synthetic pesticides.

In the genomes of two baculoviruses, Spodoptera exigua and S. littoralis multicapsid nucleopolyhedroviruses (SeMNPV and SpliMNPV, respectively), an open reading frame (ORF) encoding the large subunit of ribonucleotide reductase (RR1) was identified. The predicted amino acid sequences of SeMNPV and SpliMNPV RR1 showed high homology to RR1 proteins from eukaryotes (ca. 70% and 80% similarity, respectively). The amino acid residues thought to be involved in catalytic function were conserved in the baculoviral RR1 ORFs. The RR1 ORFs in SeMNPV and SpliMNPV were located in different genomic positions. In SeMNPV, the RR1 ORF was located upstream of the polyhedrin gene, in an anti-genomic orientation. In SpliMNPV, the RR1 ORF preceded the p74 gene. By searching databanks, sequences homologous to the N terminus of RR1 were also detected upstream of the polyhedrin gene of three other baculoviruses, Mamestra brassicae multicapsid NPV, Panolis flammea multicapsid NPV and Orgyia pseudotsugata si...