Camag Cbs Edition 85

CBS Edition 85
The CBS classification system
1. Reviews and books 17. Amines, amides and related nitrogen compounds
a) Books on TLC a) Amines and polyamines
b) Books containing one or several chapters on TLC b) Catecholamines and their metabolites
c) Books containing frequent TLC information spread over several c) Amino derivatives and amides (excluding peptides)
chapters of other information 18. Amino acids and peptides, chemical structure of proteins
2. Fundamentals, theory and general a) Amino acids and their derivatives
a) General b) Peptides and peptidic proteinous hormones
b) Thermodynamics and theoretical relationship 19. Proteins
c) Relationship between structure and chrom. behaviour
d) Measurement of physico-chemical and related values 20. Enzymes
e) Optimization of solvent systems 21. Purines, pyrimidines, nucleic acids and their constituents
f) Validation of methods a) Purines, pyrimidines, nucleosides, nucleotides
3. General techniques (unless they are restricted to the application b) Nucleic acids, RNA, DNA
within one or two classification sections) 22. Alkaloids
a) New apparatus/techniques for sample preparation
b) Separation material 23. Other substances containing heterocyclic nitrogen
c) New apparatus for sample application/dosage a) Porphyrins and other pyrroles
d) New apparatus/techniques for chromatogram development b) Bile pigments
e) New apparatus/techniques for pre- or postchromatographic c) Indole derivatives
derivatization d) Pyridine derivatives
f) New apparatus/techniques for quantitative evaluation e) other N-heterocyclic compounds
g) New apparatus/techniques for other TLC steps (distinguished 24. Organic sulfur compounds
from section 4)
25. Organic phosphorus compounds (other than phospholipids)
4. Special techniques
a) Automation of sample preparation/application 26. Organometallic and related compounds
b) Automation of complex chromatogram developing techniques a) Organometallic compounds
c) Automation, computer application in quantitative chromatogram b) Boranes, silanes and related non-metallic compounds
evaluation c) Coordination compounds
d) Combination of TLC with other chromatographic techniques 27. Vitamins and various growth regulators (non-peptidic)
e) Combination of TLC with other (non-chromatographic)
28. Antibiotics, Mycotoxins
techniques...MS, IR...etc.
a) Antibiotics
5. Hydrocarbons and halogen derivatives b) Aflatoxins and other mycotoxins
a) Aliphatic hydrocarbons
29. Pesticides and other agrochemicals
b) Cyclic hydrocarbons
a) Chlorinated insecticides
c) Halogen derivatives
b) Phosphorus insecticides
d) Complex hydrocarbon mixtures
c) Carbamates
6. Alcohols d) Herbicides
7. Phenols e) Fungicides
f) Other types of pesticides and various agrochemicals
8. Substances containing heterocyclic oxygen
a) Flavonoids 30. Synthetic and natural dyes
b) Other compounds with heterocyclic oxygen a) Synthetic dyes
b) Chloroplasts and other natural pigments
9. Oxo compounds, ethers and epoxides
31. Plastics and their intermediates
10. Carbohydrates
a) Mono- and oligosaccharides, structural studies 32. Pharmaceutical and biomedical applications
b) Polysaccharides, mucopolysaccharides, lipopolysaccharides a) Synthetic drugs
b) Pharmacokinetic studies
11. Organic acids and lipids c) Drug monitoring
a) Organic acids and simple esters d) Toxicological applications
b) Prostaglandins e) Plant extracts, herbal and traditional medicines
c) Lipids and their constituents f) Clinico-chemical applications and profiling body fluids
d) Lipoproteins and their constituents
e) Glycosphingolipids (gangliosides, sulfatides, neutral 33. Inorganic substances
glycosphingolipids) a) Cations
b) Anions
12. Organic peroxides
34. Radioactive and other isotopic compounds
13. Steroids
a) Pregnane and androstane derivatives 35. Other technical products and complex mixtures
b) Estrogens a) Surfactants
c) Sterols b) Antioxidants and preservatives
d) Bile acids and alcohols c) Various specific technical products
e) Ecdysones and other insect steroid hormones d) Complex mixtures and non-identified compounds
14. Steroid glycosides, saponins and other terpenoid glycosides 36. Thin-layer electrophoresis
15. Terpenes and other volatile plant ingredients 37. Environmental analysis
a) Terpenes a) General papers
b) Essential oils b) Air pollution
c) Water pollution
16. Nitro and nitroso compounds d) Soil pollution
38. Chiral separations
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1. Reviews and books
85 001 J. SHERMA, (Dept. of Chem., Lafayette Coll., Easton, Pennsylvania 18042, USA): Planar Chromatography. Anal.
Chem. 2000 72, 9R-25R (2000). Selective review of the literature of TLC and HPTLC in CA from Nov. 1, 1997 to Nov.
1, 1999, divided into General Considerations (history, books, reviews; experiments; theory and fundamental studies,
chromatographic systems (stationary and mobile phases;); apparatus and techniques; detection and identification of
separated zones; quantitative analysis; preparative layer chromatography and radio-thin-layer chromatography) and
applications (acids and phenols; amino acids, peptides, and proteins; antibiotics; bases and amines; carbohydrates;
dyes and pigments, hydrocarbons; lipids; pesticides; pharmaceuticals, drugs, and alkaloids; purines, pyrimidines, and
nucleic acids; steroids; surfactants and detergents; toxins; vitamins; miscellaneous organic compounds; inorganic and
metal organics).
1
2. Fundamentals, theory and general
85 002 F.P. COSSIO*, A. ARIETTA, V.L. CEBOLLA, L. MEMBRADO, M.P. DOMINGO, P. HENRION, J. VELA, (*Euskal
Herriko Unibertsitatea, Kim. Fak., PK 1072, San Sebastian 20080, Spain; qopcomof@sq.ehu.es): Enhancement of
fluorescence in thin-layer chromatography induced by the interaction between n-alkanes and an organic
cation. Anal. Chem. 72, 1759-1766 (2000). TLC of n-alkanes and of components with partially aliphatic structure (e.g.
fatty acids, cholesterol, prostaglandins, ceramides, geraniol, and propylene glycol) on berberine-impregnated silica with
n-hexane. Densitometry in the fluorescence mode at 356 nm. Proposal of an explanation for the alkane-mediated
enhancement of berberin fluorescence: The alkanes create an apolar microenvironment for the excited berberine cation,
which lowers the dielectric constant around berberine and hinders alternative relaxation mechanisms, thus favoring
fluorescence emission. This interaction of the berberine cation is much stronger than in the case of uncharged
p-acceptor molecules like caffeine and tetranitrofluorenone. Analytes with a partially aliphatic structure give also an
increase in fluorescence signal.
2a, 5, 11
85 003 T. CSERHATI*, E. FORGACS, S. BALOGH, (*Inst. for Chem., Chem. Res. Cent., Hung. Acad. of Sci., P.O. Box 17,
H-1525 Budapest, Hungary): Relationship between the reversed-phase retention of monotetrazolium and
ditetrazolium salts and their physicochemical properties. J. Planar Chromatogr. 12, 446-451 (1999). Determination
of the lipophilicity and the specific hydrophobic surface area of seven monotetrazolium and nine ditetrazolium salts by
RP TLC with ethanol, THF and dioxane as organic modifiers. The nature of the organic modifier significantly influenced
the specific hydrophobic surface area. TLC of tetrazolium salts on alumina, also on alumina impregnated by overnight
predevelopment in n-hexane - paraffin oil 19:1. After drying visualization under UV and under visible light.
2d
85 004 I. DRANCA, V. COMAN*, R. CONSTANTINESCU, F. DOGAR, C. MARUTOIU, T. LUPASCU, (*Inst. of Chem. 'Raluca
Ripan', 30 Fântânele Street, RO-3400 Cluj-Napoca, Romania): Thermoanalytical study of chemically modified
stationary phases used in thin-layer chromatography. J. Planar Chromatogr. 13, 48-51 (2000). Use of the
thermoanalytical methods TG, DTG, and DTA for the study of chemically modified stationary phases (amino-,
mercapto-, n-octyl-, n-octadecyl-organosilicon modifications). Thermal analysis gives quantitative information about the
water and organic content of the phases studied. TG, DTG, and DTA can be regarded as useful techniques for
supplementary study of the chemically modified stationary phases used in TLC.
2b
85 005 E. FORGACS, T. CSERHATI, S. BALOGH, R. KALISZAN, P. HABER, A. NASAL, (Hungarian Acad. of Sciences, Inst.
of Chem., Chem. Research Center, P.O. Box 17, 1525 Budapest, Hungary): Separation of strength and selectivity of
mobile phase by spectral mapping technique. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000,
Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 183-193. The retention behavior of 7
monotetrazolium and 9 ditetrazolium salts were studied by spectral mapping technique. Tetrahydrofuran has the highest
solvent strength while the differences among ethanol, 1-propanol, 2-propanol and dioxane were relatively low. The
selectivity of tetrahydrofuran was different from those of others. Hydrophobicity and electronic parameters were equally
involved in the retention strength and selectivity indicating the mixed character of their interaction with the alumina
stationary phase and impregnated one.
2c
85 006 E. HIDVEGI, S. PERNECZKI, M. FORSTNER, (Nat. Inst. of Forensic Tox., Varanno u. 2-4, 1146 Budapest and Techn.

85 006 E. HIDVEGI, S. PERNECZKI, M. FORSTNER, (Nat. Inst. of Forensic Tox., Varanno u. 2-4, 1146 Budapest and Techn.
Univ. of Budapest, Muegyetem rkp. 3, 1111 Budapest, Hungary): Testing for simultaneous coincidence of solvent
strength and selectivity parameters of chromatographic solvent mixtures. Sz. Nyiredy, A. Kakuk (eds.): Planar
Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 219-229. OPLC of 13
benzodiazepines on HPTLC silica gel with various calculated solvent mixtures up to 15 cm after a pre-run with acetone.
2c, e, 32a
85 007 G. IONITA, (Dept. of Phys. Chem., Univ. of Pitesti, 41 Gheorghe Doja, Pitesti 0300, Romania): Reversed-phase TLC
study of the interaction of some hydrazine derivatives with amino acids and BSA. J. Planar Chromatogr. 12,
421-424 (1999). Study of the interaction of fourteen hydrazines with Trp, Ser, Leu, Val, Gly, Phe and BSA by
charge-transfer reversed-phase TLC. TLC of hydrazines on silica gel impregnated with paraffin oil 19:1 solution in
n-hexane by overnight pre-development. The mobile phase was acetone- water 3:2. For the determination of the
lipophilicity the mobile phase contained the chosen amino acid in different concentrations. Detection through the strong
intrinsic color of the compounds.
2d
85 008 M. JANICKA, L. KWIETNIEWSKI, J. MATYSIAK, (M. Curie-Sklodowska Univ., Fac. of Chem., M. Curie-Sklodowska Sq.
3, 20-031 Lublin and Univ. of Agriculture, Dept. of Chem., Akademicka 15, 20-950 Lublin, Poland): Is it possible to
predict precisely solute biological activity using planar chromatography? Sz. Nyiredy, A. Kakuk (eds.): Planar
Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 237-248. Determination of
log kw values seem to be very useful for description of biological activity of organic substances (antifungal activity of
benzanilides). Very good correlation between log kw values and minimal inhibitory concentration (MIC) values for all
benzanilides and dermatophytes is observed. HPTLC of 9 benzanilides on RP-18 with methanol and its aqueous
solutions. Quantitation by absorbance measurement at 325nm.
2a
85 009 G. KOWALIK, B. KLAMA, R. OKOPSKA, T. KOWALSKA*, (*Inst. of Chem., Silesian Univ., 9 Szkolna Street, 40-006
Kattowice, Poland): Study of the solvolytic cleavage of octadecyl ligands from the surface of RP-18-type
stationary phases in TLC. Part 3. TLC and Raman spectroscopic quantification of the extent of cleavage for buffer pH
from 1 to 10. J. Planar Chromatogr. 13, 25-29 (2000). Investigation of the resistance of RP-18 stationary phases to
cleavage of the chemical bond coupling the octadecyl ligands to the silica matrix by repeated developments with
methanol-water buffer solutions (from pH 1-10), firstly by indirect measurement after one, two, four and eight passages
of the aforementioned solutions through the layer, by monitoring the retention of an homologous series of higher fatty
acids. The second procedure consisted in application of laser Raman spectroscopy. It was demonstrated that the
cleavage of octadecyl ligands is not a negligable process, but that the extent of cleavage was quite substantial - usually
20% - at all pH other than neutral.
2c
85 010 G. KOWALIK, K. ROGOSZ, K. BOJARSKA, T. KOWALKA*, (*Inst. of Chem., Silesian Univ., 9 Szkolna Street, 40-006
Kattowice, Poland): The solvolytic cleavage of octadecyl ligands from the surface of RP-18-type stationary
phases in TLC. Part 2. Characterization by Raman spectroscopy. J. Planar Chromatogr. 12, 429-434 (1999).
Establishment of a simple yet reliable method for quantification of the amount of solvolytic cleavage of octadecyl ligands
from the surface of RP-18 TLC stationary phases under the influence of the strongly acidic or strongly basic mixed
mobile phases comprising methanol, water, and a suitable buffer solution (pH either 3 or 9).
2d
85 011 L. LITVINOVA*, N. BEL'NIKEVICH, E. TYIHAK, (*Inst. of Macromolecular Compounds of the Russian Acad. of Sci.,
Bolshoi pr. 31, 199004 St. Petersburg, Russia): Effect of thermodynamic quality of the mobile phase on the
behavior of macromolecules in adsorption chromatography. J. Planar Chromatogr. 13, 149-151 (2000). TLC of
polystyrene of average molecular weights of 117, 240 and 460 kDa on silica gel by one-dimensional single
development in an N-type unsaturated chamber with cyclohexane - toluene - methyl ethyl ketone or cyclohexane -
toluene. Details and detection as described in J. Planar Chromatogr. 11, 114-118 (1998).
2d
85 012 I. MALINOWSKA, (Dept. of Adsorption and Planar Chromatography, M. Curie-Sklodowska Univ., Pl M.

Curie-Sklodowska3, 20-031 Lublin, Poland): The influence of electric fields on the chromatographic process in
TLC. J. Planar Chromatogr. 12, 408-415 (1999). Investigation of the influence of a perpendicular homogeneous
electric field on the TLC process for four different sorbents, SiO2, Al2O3, cellulose and polyamide; description of the
influence on mobile phase migration time, on chromatographic parameters, and on the properties of the sorbent layers
developed with monocomponent mobile phase, from apolar solvents such as hexane and heptane to strong polar
phases such as methanol, formamide and water, using two substance groups as samples - inactive compounds
(polycyclic hydrocarbons) and substances with active substituents (quinoline derivatives).
2c, 36
2c, 36
85 013 A. PELANDER, J. SUMMANEN, T. YRJOENEN, H. HAARIO, I. OJANPERÄ, H. VUORELA*, (*Div. of Pharmacogn.,

Dept. of Pharm., P.O. Box 56, FIN-00014 Univ. of Helsinki, Finland): Optimization of separation in TLC by use of
desirability functions and mixture designs according to the 'PRISMA' method. J. Planar Chromatogr. 12,
365-372 (1999). Development of a computer program for optimization of mobile phase composition in TLC and
examination of this method for chromatography of cyanobacterial hepatotoxins on normal-phase TLC plates and for
phenolic compounds on RP layers. HPTLC of microcystins (cyclic heptapeptides) on silica gel with combinations of
n-propanol - ethyl acetate - water. Densitometry at 240 nm. TLC of phenolic acids (catechin, ellagic acid, gallic acid,
kaempferol, methyl gallate, rutin) on RP-18. Videodensitometry at 254 nm.
2e, 32
85 014 G. VUCKOVIC*, S.B. TANASKOVIC, T.J. JANJIC, D. MILOJKOVIC-OPSENICA, M.B. CELAP, (*Fac. of Chem., Univ. of
Belgrade, P.O. Box 158, 11001 Belgrade, Yugoslavia): Effect of increasing the bidentate chelate ring size in Co(III)
complexes on their behavior in salting-out TLC on different adsorbents. J. Planar Chromatogr. 12, 461-465
(1999). Investigation of the effect of enlargement of the chelate ring size in Co(III) complexes containing bidentate
aminocarboxylato and/or diamine ligands on their Rf values in salting-out chromatography on thin layers of silica gel,
cellulose, polyacrylonitrile, and CN-modified silica gel. Development with aqueous solutions of ammonium sulfate.
Detection by spraying with freshly prepared saturated aqueous solutions of sodium sulfide.
2c
85 015 X. ZHOU, L. LIN*, J. ZHANG, J. CHEN, L. WANG, (*4-2, 35 Xian Street, Xigang District, Dalian 116011, P.R. China): A
preliminary study of the quantitative structure-retention relationship of gensenosides in normal phase
thin-layer chromatography. Chinese J. Chromatogr. (Sepu) 18 (3), 206-211 (2000). Determination of the TLC
retention on silica with chloroform - ethyl acetate - methanol - water 15:40:22:10. Calculation of 17 structure parameters
of the 10 ginsenosides, including topologic indices and physicochemical properties. A novel parameter "E" was
presented based on the differences of the parent substances of which the ginsenosides are composed. Three
parameters (Aw, oB and E) were selected by optimization and used in the derived multi-parameter regression equation.
Discussion of the relationship between the retention and the molecular structures (QSRR).
2c, 14
3. General techniques
85 016 S. ARTTAMANGKUL, M.H. MAHESH, R.P. HAUGLAND*, Z. DIWU, J. LIU, D. H. KLAUBERT, R. P. HAUGLAND,
(*Molecular Probes, Inc., 4849 Pitchford Avenue, Eugene, Oregon, 97402-9165; RosariaHaugland@probes.com):
5-(pentaflourobenzoylamino)fluorescein - A selective substrate for the determination of glutathione
concentration and glutathione S-Transferase activity. Anal. Biochem. 269, 410-417 (1999). Quantitative analysis of
glutathione (GSH) or of glutathione S-Transferase (GST) activity using a new fluorescent molecule,
5-(pentaflourobenzoylamino)fluorescein (PFB-F). TLC of the formed glutathione adduct, GS-TFB-F, and of excess
PFB-F on silica gel 60F254 with 1-butanol - methanol - water 3:1:1. The GS-TFB-F adduct spots were quantified with a
fluorescence plate scanner using the blue fluorescence/chemifluorescence (480 nm) mode. Detection limits for GSH
and for GST activity were 10 pmol/(l and 1 ng/(l, respectively.
3e, 18a, 20, 32
85 017 J. BARISKA, T. CSERMELY, S. FUERSR, H. KALASZ*, M. BATHORI, (*Dept. of Pharm. and Pharmacotherapy,
Semmelweis Univ. of Med., Nagyv·rad tÈr 4, P.O. Box 370, H-1445 Budapest, Hungary): Displacement thin-layer
chromatography. J. Liqu. Chromatogr. 23, 531-549 (2000). Description of the basic features of planar displacement
chromatography, including differences from elution chromatography, and that of column displacement chromatography,
the possibility of using spacer displacement chromatography. Parameters limiting displacement chromatography, and
their optimization to achieve better separation are discussed. Displacement thin-layer chromatography has the
advantage of planar arrangement of the stationary phase, and opens new possibilities for analytical separations.
3d
85 018 V.G. BEREZKIN, I. MALINOWSKA, J.K. ROZYLO*, A.B. MAKAROV, R.G. MARDANOV, (*Fac. of Chem., Dept. of
Adsorption and Planar Chromatography, M. Curie-Sklodowska Univ., M. Curie-Sklodowska Sq. 3, 20-031 Lublin,
Poland): The possible use of filter paper as a concentrating medium in TLC. J. Planar Chromatogr. 13, 82-87
(2000). Description of the possibility of applying mixtures of substances to TLC plates by use of different types of filter
(chromatographic) paper as concentrating zones. The resolution of achromatographic systems has been compared
after use of different modes of solute application - applicator (point and linear application), micropipet, use of plates with
concentrating zone, and use of different filter papers as concentrating zone. The proposed use of paper as a
concentrating zone increases the resolution of a chromatographic system compared with a micropipet; resolution is
comparable with that obtained on commercially available plates with concentrating zones.

3c
85 019 J. FLIEGER, H. SZUMILO, (Dept. of Inorg. and Anal. Chem., Med. Acad., 20-081 Lublin, Staszica 6, Poland): The
structure and chromatographic behavior of silica gel modified with metal salts. J. Planar Chromatogr. 12,
320-327 (1999). Examination of the structure and homogeneity of silica gel layers impregnated with copper(II) sulfate by
atomic-force microscopy, energy-dispersive spectrometry, and densitometry. Silica gel modified with Cu(II), Ni(II), and
Zn(II) salts was used for model analysis of anilines, quinolines, and phenol derivatives; the effect of the mobile phases
used (dioxane, ethyl acetate, benzene, dichloromethane and chloroform) on the retention of the compounds was
compared on unmodified silica gel and on the sorbent modified with metal salts.
3b
85 020 LIN JI*, BINGLIANG FANG, J.A. ROTH, (Univ. Texas, MD Anderson Canc. Ctr., Dept. Thorac. & Cardiovasc. Surg.,
Sedt. Thorac. Mol. Oncol., 1515 Holcombe Blvd., Houston, TX 77030, USA; lji@notes.mdacc.tmc.edu): A rapid,
sensitive, and nonradioactive method for assay of FHIT Ap3A hydrolase activity by fluorescence thin-layer
chromatographic image analysis. Anal. Biochem. 271, 114-116 (1999). TLC of 10 to 20 (l of the assay mixture
containing the SYBR Green II-bound reaction products AMP and ADP on silica with dioxane - NH3 - water 6:1:4. The
resulting fluorescence image was directly scanned and quantitated.
3e, 21a, 32f
85 021 C.W. MABOUNDOU, P.-Y. GROSSE, P. DELVORDRE, N.VERMERIE*, (*Service de Pharmacy, C. H. U. Jean Verdier,
APHP, avenue du 14 juillet, 93143 Bondy cedex, France): Qualitative and quantitative evaluation of a new and
economical thin-layer chromatography manual multi-applicator. J. Planar Chromatogr. 12, 373-377 (1999).
Description of a new multi-applicator for simultaneous deposition of up to 15 samples, successively on several plates of
different sizes. Acepromethazine was used as test analyte, and plates were scanned at 375 nm. The multi-applicator
furnished reliable results, both qualitatively - good inter-capillary reproducibility, and adaptibility - and quantitatively -
satisfactory linearity, reproducibility, correlation with an autosampler.
3c
85 022 I. MALINOWSKA, (Fac. of Chem., Dept. of Adsorption and Planar Chromatography, M. Curie-Sklodowska Univ., M.
Curie-Sklodowska Sq.3, 20-031 Lublin, Poland): The influence of electric fields on surface interactions in
adsorption TLC. Part I. J. Planar Chromatogr. 13, 4-8 (2000). Demonstration of the influence of electric fields on a
chromatographic system, in particular the adsorbents SiO2 and Al2O3 in electrochromatography, one of the newest
chromatographic methods. One property characterizing chromatographic adsorption systems is the surface free energy.
A thin layer wicking technique was used to determine the solid surface free energy components for Merck precoated
silica and alumina plates.
3d, 36
85 023 M. PROSEK, (Nat. Inst. of Chem., Hajdrihova 19, 1000 Ljubljana, Slovenia): My favourite separation method. Sz.
Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med.
Plants, p. 89-95. : A quick, low-priced evaluation system, consisting of a flat bed scanner and a suitable software, is
demonstrated. As example lactose in animal feed is determined on silica gel.
3f, 10a
85 024 C. SARBU, *S. COBZAC, (*"Babes-Bolyai" Univ., Fac. of Chem. and Chem. Eng., Arany Janos 11, 3400 Cluj-Napoca,
Romania): Calibration in quantitative TLC based on weighed regression functions. J. Liq. Chromatogr. 23,
273-280 (2000). Description of two methods, one on the basis of relative error in yi (YWLS) and another on the basis of
1/xi as a weighting factor (XWLS), for the calculation of calibration functions and comparison with conventional ordinary
(OLS) and simple weighted least square method (WLS). Instead of the coefficient of correlation, six coefficients of
quality are used to characterize the quality of the calibration functions. - TLC of atrazine, propazine and simazine on
silica gel using toluene - acetone 17:3. Detection under UV 254 nm; quantitation by densitometry in reflectance mode at
225 nm.
3f, 29d
4. Special techniques
85 025 A. CRECELIUS, M.R. CLENCH*, D.S. RICHARDS, J. MATHER, V. PARR, (*Div. of Chem., School of Sci. and Math.,
Sheffield Hallam Univ., Pond Street, Sheffield, UK): Analysis of UK-224,671 and related substances by thin-layer
chromatography - matrix assisted laser-desorption ionization - time of flight mass spectrometry. J. Planar
Chromatogr. 13, 76-81 (2000). Report of the generation and characterization of an impurity profile of a mixture of a
pharmaceutical compound (UK 224671) and known related impurities which were separated by TLC. The plate was
prepared for TLC-MALDI-TOFMS by electrospraying the surface with a solution of a-cyano-4-hydroxycinnamic acid by
means of an electrospray deposition device. TLC on silica gel with dichloromethane - methanol - NH3 (35%) 60:10:1.

Visualization by UV 254 nm.
4e
85 026 B. DALMADI KISS, I. KLEBOVICH, E. MINCSOVICS, K. BALOGH NEMES, (EGIS Pharmaceuticals Co. Ltd., Dept. of
Pharmacokinetics, Kereszturi 30-38, 1106 Budapest, and OPLC-NIT Co. Ltd., Andor ut 60, 1119 Budapest, Hungary):
Application of OPLC-DAR and HPLC-RD techniques in metabolite research. Sz. Nyiredy, A. Kakuk (eds.): Planar
Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 57-65. Combined
application of OPLC-DAR (overpressured layer chromatography - digital autoradiography) and HPLC-RD (high
performance liquid chromatography - radioactive detector) solves complex problems appearing during in vivo
metabolism study. OPLC-DAR is more sensitive than HPLC-RD and excellent for getting fast information about the
existence of conjugates. In case of small amount of sample with low activity, OPLC-DAR is the only possibility for
getting qualitative and quantitative information.
4b
85 027 S. EBEL, T. HENKEL, (Univ. of W¸rzburg, Dept. of Pharm., Am Hubland, 97074 Würzburg, Germany): Evaluation in
HPTLC using imaging systems 1. Basic algorithms of image processing. Sz. Nyiredy, A. Kakuk (eds.): Planar
Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 107. Sz. Nyiredy, A. Kakuk
(eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 107. Basic
principles of image processing in HPTLC (pixels, color-systems and imaging operations), definitions of scanning
densitometry and two-dimensional digitized chromatograms are demonstrated.
4c
85 028 E. MINCSOVICS, (OPLC-NIT Ltd., Andor u. 60, 1119 Budapest, Hungary): Efficiency characteristics of off-line
infusion and conventional transfusion OPLC. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000,
Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 109-119. A new off-line OPLC operation called
infusion OPLC dramatically reduces the disturbing effect of front of total wetness. Moreover, pre-wetting the layer with a
small amount of weak non eluting eluent prior to separation eliminates it totally. Both variations yield more efficient
separation compared with conventional transfusion off-line OPLC.
4b
85 029 SZ. NYIREDY, B. SZABADY, (Res. Inst. for Med. Plants, Lupaszigeti ut 4, 2011 Budapest, Hungary): Coupled layers
and multi-dimensionality in planar separations. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000,
Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 121-132. A variety of modes of multidimensional
separation in TLC are shown. Two-dimensional or multidimensional planar chromatography, two-dimensional
development on bilayers, coupled layers with stationary phases of decreasing polarity and combination of
multidimensional planar chromatographic methods are demonstrated. The greatest strength of multidimensional
separation is that compounds are distributed widely over two-dimensional space of high zone capacity. A zone capacity
of ca. 1500 could be achieved. Image analyzers might improve quantitation in future.
4b, d
85 030 M. OHNISHI-KAMEYAMA*, T. NAGATA, (*Nat. Food Res. Inst., Min. of Agr., Forestry and Fisheries, Tsukuba, Ibaraki
305, Japan): An application of X-ray fluorescence spectrometry to the study of thin-layer chromatography. Anal
Chem. 70, 1916-1920 (1998). The combination of TLC with X-ray fluorescence without any interface enables the direct,
nondestructive visualization of elements separated on a TLC plate. In addition to inorganic compounds, organics like
phenolic compounds containing chlorine, bromine, or iodine were distinctly detected by individual elemental imaging.
TLC of inorganic metal acetates and FeCl3 and MgCl2, protoporphyrin and the metal complexes (Mn and Co),
chlorinated pesticide standards, pentachlorophenol, pentabromophenol, 2,4,6-triiodophenol on e.g. cellulose and silica
gel with suitable solvent systems. After drying the plate was wrapped in polyethylene film and pasted on a sample
stage of 100 x 100 mm2 using two-sided adhesive tape. XRF was conducted using an X-ray analytical microscope.
4e
85 031 E. TYIHAK, E. MINCSOVICS, (Plant Protection Institute, Hungarian Acad. of Sciences, P.O. Box 102, 1525 Budapest,
and OPLC-NIT Co. Ltd., Andor ut 60, 1119 Budapest, Hungary): Future of OPLC. Sz. Nyiredy, A. Kakuk (eds.):
Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 37-44. OPLC
provides efficient separation of different substance groups and the attractive semi-preparative isolation of substances of
different type. Characteristics of mobile phase flow and efficiency as well as potentialities of the off-line and on-line
mode are shown.
4b
5. Hydrocarbons and halogen derivatives
85 032 F.P. COSSIO, A. ARRIETA, V.L. CEBOLLA, L. MEMBRADO, M.P. DOMINGO, P. HENRION, (Kimika Fakultatea,

85 032 F.P. COSSIO, A. ARRIETA, V.L. CEBOLLA, L. MEMBRADO, M.P. DOMINGO, P. HENRION, (Kimika Fakultatea,
Euskal Herriko Unibertsitatea, P. K. 1072, 20080 San Sebasti·n-Donostia, Spain): Enhancement of fluorescence in
thin-layer chromatography induced by the interaction between n-alkanes and organic cation. Anal. Chem. 72,
1759-1766 (2000). TLC of n-alkanes on silica gel and on berberine sulfate and 2,4,5,7-tetranitro-9-fluorenone
impregnated silica gel (impregnation with a methanolic solution of 2 - 6 mg/100 ml of the corresponding compound for
20 s). Developed with n-hexane. Detection by densitometry at 365 nm. - An ion - molecule interaction between alkanes
and berberine sulfate is considered as being responsible for the enhancement of fluorescence produced by alkanes.
5a
85 033 T.H. DZIDO, D. POLIT, (Med. Acad., Depart. of Inorg. and Anal. Chem., Staszica 6, 20-081 Lublin, Poland): Retention
parameters of aromatic hydrocarbons with polar groups in binary reversed phase thin-layer chromatography.
Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med.
Plants, p. 11-18. HPTLC of 45 aromatics with polar groups on RP18 and RP18 W with various binary solvent systems.
One mobile phase component was water and the organic one was 70% methanol, 55% acetonitrile or 55%
tetrahydrofuran. The smallest selectivity changes are observed between methanol and acetonitrile systems. Distinctive
selectivity changes are caused by modifiers. Ternary solvent systems seem to be advantageous for fine tuning of the
separation.
5b, 2
7. Phenols
85 034 I. MALINOWSKA*, J.K. ROZYLO, A. JAMROZEK-MANKO, (*Fac. of Chem., Dept. of Adsorption and Planar
Chromatography, M. Curie-Sklodowska Univ., M. Curie-Sklodowska sq. 3, 20-031 Lublin, Poland): Thin-layer
chromatography on chitin layers with aqueous mobile phase. J. Planar Chromatogr. 13, 37-41 (2000). TLC of
phenol derivatives (2-amino-, 3-amino-, 4-amino-, o-, m-, p-nitrophenol, o-, m-, p-cresol, 2,3-, 2,4-, 2,5-, 2,6-, 3,4-,
3,5-dichlorophenol) on chitin with water - aliphatic alcohol, water - carboxylic acid, water - mineral acid, water -
inorganic base and water - mineral salt mixtures.
7, 3b
85 035 P.A. MORCHAND, D.M. WELLER, R.F. BONSALL*, (*Inst. of Biological Chem. and Dept. of Plant Pathology,
Washington State Univ., Washington 99164, USA): Convenient synthesis of 2,4-diacetylphloroglucinol, a natural
antibiotic involved in the control of take-all disease of wheat. J. Agric. Food Chem. 48, 1888-1891 (2000). TLC of
DAPG fractions on silica gel with n-hexane - ethyl acetate 4:1 and 1:1. Visualization under UV 254 nm.
7, 28
8. Substances containing heterocyclic oxygen
85 036 J.S. CHO, S.S. YOO, T.K. CHEONG, M.J. KIM, Y. KIM, K.H. PARK*, (*Dept. of Food Sci. and Techn. and Research
Centre for New Bio-Materials in Agriculture, Seoul Nat. Univ., Suwon 441-744, Korea): Trans-glycosylation of
neohesperidin dihydrochalcone by bacillus stearothermophilus maltogenic amylase. J. Agric. Food Chem. 48,
152-154 (2000). TLC of maltodextrin of glucose, maltose, maltotriose, maltosyl NHDC, on silica gel with chloroform -
methanol - water 13:7:2. Detection under UV 254 nm. The plates were sprayed with 0.3%
N-1-naphthylethylenediamine, 5% sulfuric acid in methanol and heated for 5 min at 110°C.
8a
85 037 A. DEGENHARDT, H. KNAPP, P. WINTERHALTER*, (*Inst. für Lebensmittelchemie, Technische Universität

Braunschweig, Schleinitzstr. 20, 38106 Braunschweig, Germany): Separation and purification of anthocyanins by
high-speed countercurrent chromatography and screening for antioxidant activity. J. Agric. Food Chem. 48,
338-343 (2000). TLC of anthocyanidines on cellulose with n-butanol - acetic acid - water 4:1:5; upper phase).
8b
85 038 D. ENGELMEIER, F. HADACEK, T. PACHER, S. VAJODAYA, H. GREGER*, (*Comparative Phytochemistry Dept., Inst.
of Botany, Univ. of Vienna, Rennweg 14, A-1030 Vienna, Austria): Cyclopenta[b]benzofurans from Aglaia species
with pronounced antifungal activity against rice blast fungus (Pyricularia grisea). J. Agric. Food Chem. 48,
1400-1404 (2000). Preparative TLC of rocaglaol, aglafolin, rocaglamide, desmethyl rocaglamide on silica gel with
dichloromethane - ethyl acetate - methanol 10:14:1. Visualization under UV 254 nm.
8b
85 039 A. JAMSHIDI*, M. ADJVADI, S.W. HUSAIN, (*Pharm. Res. Center, P.O. Box 13185/877, Teheran, Iran):

85 039 A. JAMSHIDI*, M. ADJVADI, S.W. HUSAIN, (*Pharm. Res. Center, P.O. Box 13185/877, Teheran, Iran):
Determination of kaempferol and quercetin in an extract of Ginkgo biloba leaves by high-performance thin-layer
chromatography (HPTLC). J. Planar Chromatogr. 13, 57-59 (2000). HPTLC of kaempferol and quercetin on silica gel
with toluene - acetone - methanol - formic acid 46:8:5:1. After drying under nitrogen and heating at 40°C for 5 min
densitometry at 254 nm. RSD 1.37 and 1.40 for kaempferol and quercetin respectively.
8a, 32e
85 040 Z. MALES*, M. MEDIC-SARIC, (*Dept. of Pharmacognosy, Fac. of Pharm. and Biochem., Univ. of Zagreb, Marulicev trg
20/II, 10000 Zagreb, Croatia): Investigation of the flavonoids and phenolic acids of Sambuci flos by thin-layer
chromatography. J. Planar Chromatogr. 12, 345-349 (1999). Investigation of the efficiency of ten TLC systems (of
mobile phases) for the separation of flavonoids and phenolic acids by three mathematical techniques: calculation of the
information content derived from Shannon's equation; determination of discriminating power and formation of clusters
and dendrogram. TLC of e.g. ferulic acid, caffeic acid, chlorogenic acid, isoquercitrin, hyperoside, rutin) on silica gel
with 10 different mobile phases, the most suitable of which were ethyl acetate - methanol - formic acid - water 200:27:
5:20 and ethyl acetate - formic acid - water 8:1:1. Visualization by spraying with 1% methanolic
diphenylboryloxyethylamine, then 5% ethanolic polyethylene glycol 4000. Evaluation under UV 366 nm.
8a
85 041 T. MASUDA*, S. MIZUGUCHI, T. TANAKA, K. IRITANI, Y. TAKEDA, S. YONEMORI, (*Fac. of Integrated Arts and Sci.,
Univ. of Tokushima, Tokushima 770-8502, Japan): Isolation and structure determination of new oxidative ferulic
acid glucoside esters from the rhizome of Alpinia speciosa, a Zingiberaceae plant used in Okinawa food
culture. J. Agric. Food Chem. 48, 1479-1484 (2000). TLC of Trolox (epicatechin, ethyl 4-O-feruloyl-b-glucopyranoside
and 4-hydroxy-3-methoxyphenyl 4-O-feruloyl-b-glucopyranoside) on silica gel with methanol - dichloromethane 1:9, 1:4
and 1:3. Detection by spraying with a 3% solution of linoleic acid in hexane, continuos irradiation by UV of 254 nm and
observation of fluorescent emission light from each sample spot every 15 min.
8b
85 042 M. MORIMATO*, S. KUMEDA, K. KOMAI, (Dept. of Agr. Chem., Kiuki Univ., Nakamachi, Nara 3327-204, Japan):
Insect antifeedant flavonoids from Guaphalium affine D. Don. J. Agric. Food Chem. 48, 1888-18891 (2000). TLC
of polymethylated flavones, chalcone derivatives, tangeritine. Bioautography procedure using larvae.
8a
85 043 H.D. SMOLARZ*, G. MATYSIK, M. WOJCIAK-KOSIOR, (*Dept. of Pharm. Botany, Med. Acad., Staszica 6, 20-081
Lublin, Poland): High-performance thin-layer chromatographic and densitometric determination of flavonoids in
Vaccinium myrtillus L. and Vaccinium vitis-idaea. L. J. Planar Chromatogr. 13, 101-105 (2000). HPTLC of flavonoids
(i. a. quercetin, avicularin, hyperin, isoquercetin) on silica gel by two- and three-step gradient elution with mixtures of
toluene, hexane, ethyl acetate and methanol for glycosides and multiple development for aglycones.
Videodocumentation was achieved by use of videoscanning equipment. Visualization under UV 254 or 365 nm; or by
spraying with one of the three reagents: (i) 10% KOH in methanol; (ii) 1% solution of FeCl3; and (iii) Gibbs reagent.
Quantitation by densitometry at 254 nm.
8a
85 044 N. ZHU, T.-C. HUANG, Y. YU, E. J. LaVOIE, C. S. YANG, C.-T. HO , (Dept. of Food Sci., Rutgers Univ., 65 Dudley
Road, New Brunswick, New Jersey 08901-8520, USA): Identification of oxidation products of (-)-epigallocatechin
gallate and (-)-epigallocatechin with hydrogen peroxide. J. Agric. Food Chem. 48, 979-981 (2000). TLC on silica
gel with chloroform - methanol - water 3:1:1. Visualization under UV 254 and 360 nm and with 10% sulfuric acid in
ethanol followed by heating.
8a
11. Organic acids and lipids
85 045 A. DEMIRBAS*, N. YILMAZ, (*P.K. 216, TR-61035 Trabzon, Turkey): trans-Fatty acid content of edible margerines
in Turkey. Dtsch. Lebensm. Rundsch. 96, 136-138 (2000). TLC of cis and trans isomers of methyl esters of fatty acids
(12:0, 14:0, 16:0, 18:0, 20:0, 9c-16:1, 9c-18:1, 9c, 12c-18:2, 9c, 12c, 15c-18:3) on silica gel, dipped in 12% AgNO3
solution, developed in the dark: (a) for 10 min in toluene and 5:1 ethyl acetate - acetic acid mixture 40:3; (b) 30 min in
the same solvent with a liner of 5 cm; (c) 75 min in toluene - ethyl acetate 40:3 with a liner of 10 cm. Visualization after
spraying with 2',7'-dichlorofluorescein (0.5% in methanol) under UV 350 nm. Quantitation after elution into counting vials.
11a
85 046 J.C. ESPIN*, C. SOLER-RIVAS, H.J. WIECHERS, (*Laboratorio de FiloquÌmica, Departamento Ciencia y TechnologÌa

85 046 J.C. ESPIN*, C. SOLER-RIVAS, H.J. WIECHERS, (*Laboratorio de FiloquÌmica, Departamento Ciencia y TechnologÌa
de los Alimentos, CEBAS-CSIC, P.O. Box 4195, 30080 Murcia, Spain): Characterization of the total free radical
scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J. Agric. Food
Chem. 48, 648-656 (2000). TLC of e.g. sesamol, vanillic acid, syringic acid, sinapic acid, protocatechuic acid, caffeic
acid, tyrosal, hydroxytyrosal and oleuropein on silica gel with toluene - ethyl acetate - formic acid 5:4:1. Detection after
staining with 0.4 M DPPHH in methanol.
11a
85 047 M.O. FATOPE*, A. ADOUM, Y. TAKEDA, (*Chem. Dept.; Coll. of Sci., Sultan Quaboos Univ., P.O. Box 36, Postal Code
123, Al-Khod, Sultanate of Oman): C18 acetylenic fatty acids of Ximerica americana with potential pesticide
activity. J. Agric. Food Chem. 48, 1872-1874 (2000). Analytical and preparative TLC of e.g. octadeca-5-yneoic acid,
10Z, 14E, 16E-octadeca-10,14,16-triene-12-ynoic acid on silica gel with petroleum ether - ethyl acetate 1:1, and
chloroform - ethyl acetate 4:1. Visualization under UV or by exposure to iodine vapors.
11a
85 048 P. MIGAS, W. CISOWSKI, (Med. Univ. of Gdansk, Dept. of Pharmacogn., ul. Gen. J. Hallera 107, 80-416 Gdansk,
Poland): TLC-MGD analysis of phenolic acids from tissue cultures of vaccinium corymbosum var. bluecrop. Sz.
Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med.
Plants, p. 171-172. Multiple gradient development (MGD) of (ferulic, caffeic, chlorogenic, protocatechuic, p-coumaric,
o-coumaric, m-coumaric, syringic, synapic, p-hydroxybenzoic, gallic, elagic, isochlorogenic acid) in plants on silica gel,
amino, cyano, RP18W and diol plates with mobile phases of variant range of polarity. This method is useful for the
analysis of plant extracts rich in many substances of wide polarity range.
11a, 32e
85 049 Y. PLEKHANOV (Otdelenie Molekularnoj i Radiacionnoj Biofiziki, Peterburgskij Institut Jadernoj Fiziki, Gatchina
Leningradskoj Oblasti, 188350, Russia): Rapid staining of lipids on thin-layer chromatograms with amido black
10B and other water-soluble stains. Anal. Biochem 271, 186-187 (1999). Staining of phospholipids, diacylglycerides,
free fatty acids, triacylglycerides, cerebrosides, sulfocerebrosides and cholesterol with 26 water soluble organic colors,
after TLC on silica. Most sensitive stain with 0.2% amido black 10B in 1 M NaCl: After pre-soaking in water, the
chromatogram was immersed in the staining solution for 5 - 10 min. The sensitivity is about 15 ng of diacylglycerides,
triacylglycerides, and phosphatidylserine; 100 ng of free fatty acids, and 500 ng of phorbol esters.
11c, 32
85 050 P. REDDY, E.E. MULLER, B. FRIED*, J. SHERMA, (*Dept. of Biol., Lafayette Coll., Easton, PA 18042, USA): Effects
of freezing and thawing and of formalin and ethanol fixation on HPTLC analysis of neutral lipids in Biomphalaria
glabrata snails. J. Planar Chromatogr. 12, 397-399 (1999). HPTLC of neutral lipids (i.a. cholesteryl oleate, methyl
oleate, triolein, oleic acid, cholesterol) on silica gel, after prewashing by development with dichloromethane - methanol
2 : 1, with petroleum ether - ether - acetic acid 40:10:1 in a paper lined chamber. Detection by spraying with 50%
ethanolic phosphomolybdic acid and heating at 110°C for 15 min. Quantification by densitometry at 700 nm.
11c
85 051 B. RENGER, W. ISE, H. JEHLE, M. WINKHART (Byk Gulden, Lomberg Chemische Fabrik GmbH, Byk-Gulden Str. 2,
78467 Konstanz, Germany): Planar chromatographic procedures in the quality control of Venticute(r) - a human
homologous recombinant surfactant. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred,
Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 97-105. HPTLC of Venticute on silica gel with chloroform -
methanol - acetic acid - water 65:20:8:5. Visualization with 10% copper sulfate in 4% phosphoric acid. Quantification by
absorbance measurement at 365 nm. Intermediate precision 1-4%, recovery 98-103%, limit of quantification 0.1-0.4%
(ca. 15-40 µg/spot). Immuno TLC on diol plates with chloroform - methanol - 8% NH3 65:30:6. Immunological staining
according to conventional western plot. Visualization via enhanced chemiluminescence detection system. Densitometric
evaluation via peak area of the x-ray film including background subtraction. Recovery 65-120% and 75-110%
(inter-assay, n=6, 3 concentrations), repeatability 24-28% and intermediate precision 22-52% (n=6, 3 concentrations),
detection limit 0,125 ng HCP (host cell derived proteins), quantitation limit 0,02% and specification limit 0,1%. This
HPTLC procedure can also be used to separate the hydrophobic protein from impurities and modification (which can not
be separated by HPLC). Visualization via coommassie brilliant blue (0,15 %). Repeatability ca. 10% (n=8).
11d
85 052 C.M. UTZMANN, M.O. LEDERER*, (*Inst. f¸r Lebensmittel-Chemie, Univ. Hohenheim, Garbenstr. 28, D-70593 Stuttgart,

85 052 C.M. UTZMANN, M.O. LEDERER*, (*Inst. f¸r Lebensmittel-Chemie, Univ. Hohenheim, Garbenstr. 28, D-70593 Stuttgart,
Germany): Identification and qualification of aminophospholipid linked Maillard compounds in model systems
and egg yolk products. J. Agric. Food Chem. 48, 1000-1008 (2000). Analytical TLC of the triflate derivative of
di-o-isopropylidene-2,3:4,5-b-D-fructopyranose on silica gel with chloroform - methanol - water 95:35:2; TLC of
phospholipids on silica gel with chloroform - methanol - water 65:25:4. Detection by spraying with 5% phosphomolybdic
acid in ethanol and heating for 5 min at 60°C. Also TLC separation and detection of phosphatidylethanolamine-linked
glucosylamines; Amadori products and similar compounds. Preparative TLC on silica gel with chloroform - methanol -
NH3 (25%) 75:25:1. Detection (on the plate margins) by spraying with 2,7-dichlorofluorescein solution (0.1% in
methanol).
11c
85 053 X. XU, L.B. FOMUSO, C.C. AKOH*, (*Dept. of Food Sci. and Techn., Food Sci. Building, Room 211, The Univ. of
Georgia, Athens, GA 30602-7610, USA): Synthesis of structured triacylglycerols by lipase-catalyzed acidolysis in
a packed bed bioreactor. J. Agric. Food Chem. 48, 3-10 (2000). TLC of diacylglycerol content on silica gel with
petroleum ether - diethyl ether - acetone 45:5:1. Visualization by spraying with a 0.2% methanolic solution of
2',7'-dichlorofluorecein.
11c
13. Steroids
85 054 B. BAGOCSI, G. RIPPEL, Z. VEGH, S. MAHO, K. FERENCZI-FOFOR, (Gedeon Richter Ltd., P.O. Box 27, 1475
Budapest 10, Hungary): Purity testing of norethisterone drug substance by personal OPLC. Sz. Nyiredy, A.
Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p.
151-156. Semi-quantitative OPLC of norethisterone on HPTLC silica gel with 4 mL butyl acetate - chloroform 17:3 after
a pre-run with 4 mL n-hexane. Derivatization by spraying with a 10 % ethanolic sulfuric acid solution. Semi-quantitative
evaluation at 366 nm. Impurities (6a-hydroxy-, 6b-hydroxy-, 10-hydroxy-, 6-keto-, 17-epi-norethisterone and nordione)
can be separated and detected below 0,05 %.
13
85 055 M. BATHORI, * A. GERGELY, H. KALASZ, G. NAGY, A. DOBOS, I. MATHE, (*Dept. of Pharmacogn., Albert
Szent-Gyˆgyi Med. Univ., P.O. Box 121, Eˆtvˆs u 6, H-6701 Szeged, Hungary): Liquid chromatographic monitoring of
phytoecdysteroid production of Serratula wolffii. J. Liqu. Chromatogr. 23, 282-294 (2000). Comparison of
techniques TLC of 20-hydroxyecdysone and polypodine B (5,20-dihydroxyecdysone) on silica gel with dichloromethane
- ethanol (96%) 4:1, chloroform - methanol - benzene 25:5:2, and ethyl acetate - methanol - conc. NH3 17:2:1. Detection
directly after development under UV 254 nm; quantitation by densitometry at 254 nm in reflectance mode. - Parallel
analyses using both TLC with densitometry and HPLC gave similar results.
13c
85 056 K.O. ESUOSO*, H. LUTZ, E. BAYER, M. KUTUBUDDIN, (*Inst. of Org. Chem., Res. Centre for Nucleic Acid and
Peptide Chem., Univ. of Tuebingen, 72076 Tübingen, Germany): Unsaponifiable lipid constituents of some
underutilized tropical seed oil. J. Agric. Food Chem. 48, 231-234 (2000). TLC of the unsaponifiables (hydrocarbons,
triterpene alcohols, sterols) on silica gel, bandwise applied, with hexane - ethyl acetate 6:1. Detection after spraying
with 0.5% Rhodamine 6G solution and observation under UV. After elution and extraction silanation of sterols and
triterpene alcohols with BSTFA for GC investigation.
13, 15
85 057 K. FERENCZI-FODOR, B. BAGOCSI, D. FABIAN, E. GÜRTLER, Z. VEGH, (Gedeon Richter Ltd., P.O.Box 27, 1475
Budapest 10, Hungary): Chromatographic methods in pharmaceutical purity testing: a case study for ethinyl
estradiole. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res.
Inst. for Med. Plants, p. 19-25. OPLC of ethinyl estradiole on silica gel with cyclohexane - ethyl acetate - chloroform
3:1:1. Detection by spraying with sulfuric acid and visual evaluation at 366 nm. The method is compared with the
Official British and European Pharmacopoeia monographs. Only with the OPLC method it was possible to separate all
impurities, i.e. 6-hydroxy derivatives (both isomers), the oxo-derivatives (6-oxo- and 16-oxo-), the 9(11)-dehydro and the
17-epi-derivatives from the estrone and estradiol. The HPLC method of the 3rd European Pharmacopoeia has to be
changed slightly in order to analyze all impurities. The British Pharmacopoeia TLC method was not suitable because of
its poor selectivity. Both OPLC and HPLC are suitable for purity testing. The OPLC method was preferred for
process-validation because it shows a short analysis time and low eluent consumption (tenfold less than HPLC). The
HPLC method was used for critical batches due to its better precision (3,5% RSD).
4b, 13b
85 058 S.M. PETROVIC*, M. SAKAC, S. JOVANOVIC-SANTA, (*Dept. of Anal. Chem., Fac. of Techn., Univ. of Novi Sad, Cara

85 058 S.M. PETROVIC*, M. SAKAC, S. JOVANOVIC-SANTA, (*Dept. of Anal. Chem., Fac. of Techn., Univ. of Novi Sad, Cara
Lanzara 1, P.O. Box 340, 21000 Novi Sad, Yugoslavia): Steroid structure and retention in normal- and
reversed-phase thin-layer chromatography. J. Planar Chromatogr. 13, 106-113 (2000). Study of the
chromatographic behavior of a series of (26) estradiol and estrone derivatives on silica gel with binary nonaqueous
mobile phases and on C8- and C18-modified silica with binary aqueous mobile phases. HPTLC on silica gel or silica
RP-8 resp. RP-18. Spots were observed under UV 254 nm.
13b
85 059 M. POZAR, A. GOLC WONDRA*, M. PROSEK, (*Nat. Inst. of Chem., Lab. of Food Chem., Hajdrihova 19, 1000
Ljubljana, Slovenia): TLC and HPLC determination of levels of cholesterol before and after its reduction in liquid
egg yolk. J. Planar Chromatogr. 12, 416-410 (1999). HPTLC of cholesterol on silica gel with petroleum ether - ether -
acetone 90:10:1 in an unsaturated chamber. Detection by spraying with a 10% solution of phosphomolybdic acid in
ethanol and drying at 100°C. Quantification by densitometry at 580 nm.
13c
14. Steroid glycosides, saponins and other terpenoid glycosides
85 060 M. GLENSK*, W. CISOWSKI, (*Dept. of Pharmacogn., Wroclaw Med. Univ., pl. Nankiera 1, 50-140 Wroclaw, Poland):
Two-dimensional TLC with adsorbent gradient for analysis of saponins in Silene vulgaris Garcke. J. Planar
Chromatogr. 13, 9-11 (2000). Separation of a saponin mixture by two-dimensional TLC; the first development was
performed on an RP-18w HPTLC plate and that in the perpendicular direction on a silica HPTLC plate resulting in a
separation into 18 components compared to 9 components using conventional TLC. HPTLC of a saponin mixture on
RP-18 with 1.0% aqueous formic acid - methanol 3:7 in the first direction and on silica gel with chloroform - methanol -
formic acid - water 10:4:1:1 in the second direction. Visualization by spraying with anisaldehyde sulfuric acid reagent
and heating at 110°C for 5 min, then evaluation in visible light or under UV 365 nm.
14
85 061 D.M. HOLSTEGE*, T. FRANCIS, B. PUNSCHER, M. C. BOOTH, F.D. CRALEY, (*Toxicology Lab., California Vet.
Diagnostic Lab. System, Univ. of California, Davis, CA 95616, USA): Multiresidue screen for cardiotoxins by
two-dimensional thin-layer chromatography. J. Agric. Food Chem. 48, 60-64 (2000). TLC of oleandrin, digitoxin,
gitoxin, gitoxigenin and grayanotoxins I, II, and III (spotted at different locations) on silica gel. Development in the first
direction with 25% dichloromethane in ethyl acetate, after drying development with ethyl acetate - toluene - acetic acid
6:3:1; development in the second direction with methanol, drying and development with dichloromethane - acetonitrile -
ethanol - NH3 90:20:10:1. Detection by spraying with 20% AlCl3- solution and heating for 5 min at 110°C. After the
spots were recorded the plate was sprayed with chloramine T solution and heated for 5 min at 110°C.
14
85 062 G. INDRAYANTO*, H. MARGALIN, E. RATNASARI, A. SYAHRANI, (*Lab. of Pharm. Biotechn., Fac. of Pharm., 13
Airlangga Univ., Jl. Dharmawangsa dalam, Surabaya 60286, Indonesia): Densitometric determination of
o-aminobenzoic acid, b-D-glucopyranosyl-1-o-aminobenzoate, and
O-b-D-glucopyranosyl-(1-6)-O-b-D-glucopyranosyl-1-o-aminobenzoate in cell-suspension cultures of Solanum
mammosum. J. Planar Chromatogr. 12, 456-460 (1999). TLC of OABA, OABA-glc and OABA-di-glc on silica gel with
ethyl acetate - methanol - water 7:2:1. Ascending chromatography. Quantitation by densitometry in
absorbance-reflectance mode at 332 nm. - The densitometric method is selective, precise, and accurate and can be
used for routine analysis. New simple and rapid TLC procedure.
14
85 063 R.R. KING*, C.H. LAWRENCE, L.A. CALHOUN, (*Agriculture and Agriculture-Food Canade, Potato Res. Centre, P.O.
Box 20280, Fredericton, New Brunswick, Canada E3B 4Z7): Microbial gycosylation of thaxtomin A, a partial
detoxification. J. Agric. Food Chem. 48, 512-514 (2000). TLC of thaxtomin A glucoside on silica gel with ethyl acetate
- water - acetic acid - formic acid 10:1:1:1, rechromatography on silica gel and purified further by TLC on RP-18 with
acetone - water 3:2.
14
16. Nitro and nitroso compounds
85 064 R. KRISHNAMURTHY, S.M. BAKRE, B.M. SHINDE*, (*Forensic Sci. Lab., State of Maharashtra, Santacruz (East),

85 064 R. KRISHNAMURTHY, S.M. BAKRE, B.M. SHINDE*, (*Forensic Sci. Lab., State of Maharashtra, Santacruz (East),
Mumbai, India, Pin-400 098): Simultaneous detection of high explosives in post-explosion debris by HPTLC with
two successive mobile phases. J. Planar Chromatogr. 12, 394-397 (1999). HPTLC of trinitrotoluene (TNT), cyclonite
(RDX), Tetryl, PETN, Ng-Nc on silica gel in a tank previously saturated with mobile phase for ca. 45 min; first
development with trichloroethylene - acetone 4:1 and then with petroleum ether - acetone 17:3. Detection by spraying
with ethanolic KOH resulting in a reddish-orange spot for TNT. The plate was then heated at 120°C for 10 min, cooled,
and sprayed with sulfanilic acid and a-naphthylamine reagent. Observation of other explosives.
16
17. Amines, amides and related nitrogen compounds
85 065 E. CSOMOS, ZS. KIRALY-VEGHELY, GY. KATAY, L. DIOFASI, L. SIMON-SARKADI, E. TYIHAK, (Budapest Univ. of
Techn. and Economics, Dept. of Biochem. and Food Techn., P.O. Box 91, 1052 Budapest, Hungary): Determination of
biologically active compounds in wines. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred,
Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 173-181. After prechromatographic derivatization with dansyl
chloride OPLC of biogenic amines on HPTLC silica gel with n-hexane - n-butanol - triethylamine 900:100:81 and
n-hexane - n-butanol 4:1. Densitometry at 305 nm. The main biogenic amines in red wines were putrescine (41%),
agmatine (33%), tyramine (13%) and spermidine (
17a, 18a, 7
18. Amino acids and peptides, chemical structure of proteins
85 066 E. FORGACS, (Hungarian Acad. of Sciences, Inst. of Chem., Chem. Research Center, P.O. Box 17, 1525 Budapest,
Hungary): Interaction of amino acids with hydroxypropyl-b-cyclodextrin. Sz. Nyiredy, A. Kakuk (eds.): Planar
Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 195-204. The interaction
between 18 amino acids and hydroxypropyl-b-cyclodextrin was determined by charge-transfer chromatography. The
hydrophilicity and bulkiness of amino acids determines the capacity to form inclusion complexes with
hydroxypropyl-b-cyclodextrin, e.g. the more hydrophilic amino acids are less liable to form complexes.
18a
85 067 V.B. KUMAR*, A.E. BERNARDO, M.M. ALSHAHER, M. Buddhiraju, R. PURUSHOTHAMAN, J.E. MORLEY, (*VA Med.
Center, GRECC 11G/JB, No. 1 Jefferson Barracks Dr., St. Louis, MO 63125-4199): Rapid assay for nitric oxide
synthase using thin-layer chromatography. Anal. Biochem. 269, 17-20 (1999). TLC of L-[14C] arginine and L-[14C]
citrulline on silica gel with ammonium hydroxide - chloroform - methanol - water 4:1:9:2. Identification by
autoradiography and quantification by radiometric scanning.
18a, 34
21. Purines, pyrimidines, nucleic acids and their constituents
85 068 U. BROCKSTEDT, W. PFAU*, (*Dept. Toxicol. & Environ. Med., Fraunhofer Society, Grindelallee 117, DE-20146
Hamburg, Germany): Reversed-phase high-performance liquid chromatographic separation of 32p-labeled
nucleotide adducts formed by food-derived carcinogenic aminoimidazoazarenes. Chromatographia 50, 547-552
(2000). Multi-dimensional ion-exchange TLC on PEI-cellulose TLC plate with sodium phosphate buffer (1M, pH 6.0) in
direction D1 over night. After washing with water, ammonium formate, water and drying, elution in direction D2 (in the
opposite to D1) with 3.5 M lithium formate, 8.5 M urea, pH 3.5. After washing with 1.3 mM tris, water and drying, elution
with bugger 1.2 M sodium phosphate pH 6.0 over night. Detection by autoradiography at 80°C by use of intensifying
screens. Comparison with HPLC.
21
85 069 S.A. TEIJEIRO, G.N. MORONI, M.I. MOTURA, M.C. BRINON*, (*Departamento de Farmacia, Facultad de Ciencas
QuÌmicas, Ciudad Universitaria, Universidad Nacional de Córdoba, 5000 Córdoba, Argentina): Lipophilic character of
pyrimidinic nucleoside derivatives: Correlation between shake flask, chromatographic (RP-TLC and RP-HPLC)
and theoretical methods. J. Liqu. Chromatogr. 23, 855-872 (2000). HPTLC of pyrimidinic nucleoside derivatives
(thym, ac-thym, AZT, ac-AZT, iso-AZT, uri, cyt, ac-cyt) on RP-18 with acetone - water mixtures with acetone content
between 0% and 90% in 10% increments. After drying at 40°C the plates were exposed to UV radiation.
21
22. Alkaloids
85 070 J.H. SKERRITT*, S.L. GUIHOT, S.E. McDONALD, R.A. CULVENOR, (*CSIRO Plant Industry, G.P.O. Box 1600,

85 070 J.H. SKERRITT*, S.L. GUIHOT, S.E. McDONALD, R.A. CULVENOR, (*CSIRO Plant Industry, G.P.O. Box 1600,
Canberra, ACT 2601, Australia): Develoment of immunoassays for tyramine and tryptamine toxins of Phalaris
aquatica L. . J. Agric. Food Chem. 48, 27-32 (2000). TLC of alkaloids (i. a. dimethyltryptamine, bufotenine, gramine,
b-carboline, N-methyl-tryptamine, tyramine, 5-methoxy-N,N-dimethyltryptamine, hordenine) acc. to Anderton,
(Phytochemistry 48, 437-439 (1998)). Semiquantitative estimation against standards. Excellent correlation between
ELISA and TLC results.
22
85 071 H. SZUMILO*, J. FLIEGER, (*Dept. of Inorg. and Anal. Chem., Medical Acad., 20-081 Lublin, Poland): Application of
differently modified silica gel in the TLC analysis of alkaloids. J. Planar Chromatogr. 12, 466-470 (1999). HPTLC
and TLC of alkaloids (i.a. narceine, paracodeine, morphine, codeine, thebaine, papaverine) on silica, also modified by
immersion in aqueous salt solutions; single or multiple development in sandwich chambers with i.a. toluene - methanol -
acetone 7:2:1. Detection under UV 254 nm or after spraying with Dragendorff reagent or iodoplatinate reagent.
Quantitation by densitometry.
22
85 072 Y. YANG (Yang Yan)*, ZH. Zhang (Zhang Zhongsu), X. DUAN (Duan Xinjun), (*Shanxi Provin. Inst. Drug Cont.,
Taiyuan 030001, P.R. China): (Determination of strychnine in Fabingning capsules by thin-layer
chromatography. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (6), (2000). TLC on silica gel with
toluene - acetone - ethanol - conc. NH3 30:20:3:1. Detection under UV 254 nm. Quantitation by densitometry at 254 nm.
22, 32g
85 073 W. ZWICKENPFLUG, (Walther Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität,

Nussbaumerstr. 26, D-80336 München, Germany): N-Nitrosation of mysmine yields HPB
(4-hydroxy-1-(3-pyridyl)-1-butanone) and NNN (N-nitrosonornicotine). J. Agric. Food Chem. 48, 392-394 (2000).
TLC of HPB, NNN, and myosmine on silica gel with chloroform - ethanol 9:1. Spots were analyzed by HPLC-DAD after
removal.
22
23. Other substances containing heterocyclic nitrogen
85 074 D. MATOSIUK*, K. JOZWIAK, (*Dept. of Synthesis and Techn. of Drugs, Fac. of Pharm., Med. Univ., Staszica 6,
20.081 Lublin. Poland): Determinationof the lipophilicity of some carbonyl derivatives of 2-aminoimidazolines-2
by reversed-phase thin-layer chromatography. Part 2. The lipophilicity of
1,6-diaryl-5,7(1H)dioxo-2,3-dihydroimidazo[1,2-a][1,3,5]triazines and
1-aryl-5,6(1H)dioxo-2,3-dihydroimidazo[1,2-a]imidazoles. J. Planar Chromatogr. 13, 130-134 (2000). Determination of
the lipophilicity of twenty five new carbonyl derivatives of 1-aryl-2-aminoimidazolines-2 (twenty
1,6-diaryl-5,7(1H)dioxo-2,3-dihydroimidazo[1,2-a][1,3,5]triazines and five
1-aryl-5,6(1H)dioxo-2,3-dihydroimidazo[1,2-a]imidazoles) by RPTLC on commercial RP-18 silica gel plates with
methanol - acetic acid - water. RM values for all the compounds were linearly dependent on mobile phase methanol
concentration within the range of 60 to 90%.
23, 2c
27. Vitamins and various growth regulators
85 075 T.S. PANJAITAN, A. SYAHRANI, G. INDRAYANTO*, (*Lab. of Pharm. Biotechn., Fac. of Pharm., Airlangga Univ., Jl.
Dharmawangsa dalam, Surabaya 60286, Indonesia): Densitometric determination of p-aminobenzoic acid and
b-D-glucose-1-p-aminobenzoate in cell-suspension cultures of Solanum laciniatum. J. Planar Chromatogr. 13,
114-118 (2000). TLC of PABA and PABA-glc on silica gel with ethyl acetate - methanol - water 77:13:10. Ascending
development. Quantitation by densitometry at 272 nm (absorbance-reflectance). The densitometric method is simple
and rapid and can be used for routine analysis of plant-cell cultures.
27
28. Antibiotics, Mycotoxins
85 076 L. BOTZ, S. NAGY, B. KOCSIS, GY. HORVATH, (Univ. of Pecs, Dept. of Pharmacy/Internal Medicine/Medical

85 076 L. BOTZ, S. NAGY, B. KOCSIS, GY. HORVATH, (Univ. of Pecs, Dept. of Pharmacy/Internal Medicine/Medical
Microbiology and Immunology/Botany, Honved u. 3, 7624 Pecs, Hungary): Planar chromatographic aspects of direct
bioautography. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000,
Res. Inst. for Med. Plants, p. 77-87. Screening methods for natural products with antibacterial activity are shown, i.e.
diffusion, dilution and bioautographic methods as well as direct bioautography. Chromatographic influences of direct
bioautography are demonstrated.
28a.32e
85 077 I.M. CHOMA, (M. Curie-Sklodowska Univ., Dept. of Chem. Phys. and Physicochem. Methods of Separation, M.
Curie-Sklodowska Sq. 3, 20-031 Lublin, Poland): Determination of four tetracyclines in milk by a simple
Thin-Layer Chromatographic method. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred,
Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 159-169. HPTLC of tetracyclines on silica gel (with
concentration zone, impregnated with 5% aqueous Na2EDTA solution) with the lower layer of chloroform - methanol -
5% aqueous Na2EDTA solution 13:4:1 after a pre-development with n-hexane and acetone to remove lipid fractions.
Detection of fluorescent spots at 254 and 366 nm. Detection limit is found to be 0,1 µg (twofold development with
mobile phase). At 0,05 µg level tetracycline and chlorotetracycline migrate together.
28
85 078 R. EYMANN, H.E. HAUCK, (Merck KGaA, Frankfurter Str.250, 64271 Darmstadt, Germany): Chrom Biodip(r)
antibiotics - a high-performance bioautographic test kit. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography
2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 67-76. HPTLC of extracted feed sample on
silica gel with different solvent systems: (1) acetonitrile, (2) acetonitrile - dichloromethane 49:1, (3) ethyl acetate -
methanol - NH3 30:10:1. Bioautographic detection of antibiotics. (Description in this CBS p. 10.)
28a, 37c, 32e
85 079 L. LIN*, J. ZHANG, Y. WANG, X. ZOU, (*Dalian Inst. of Chem. Phys., Chinese Acad. of Sci., Dalian 116012, P. R.
China): Quantitative determination of tetrodotoxin by thin-layer chromatography. J. Planar Chromatogr. 12,
388-391 (1999). New precise procedure for quantitative determination. TLC of tetrodotoxin on silica gel with the
optimized mobile phase n-butanol - acetic acid - water 2:1:1. Detection by immersion in a solution of potassium
hydroxide in ethanol, drying and heating at 1608C for 20 min. Quantitation by scanning in fluorescence mode at
366/>400 nm. Linearity range 40-1200 ng; RSD for five replicate determinations was smaller or equal 3% within plate
and smaller or equal 5.0% plate-to-plate.
28b
85 080 E. PAPP*, B. BAGOCSI, K. H-OTTA, L. KOVACSIS-ACS, E. MINCSOVCS, (*Dept. of Chem. Techn. and Env. Chem.,
L. Eötvös Univ., P.O. Box 32, H-1518 Budapest 112, Hungary): The role of over-pressured-layer chromatography
among chromatographic methods for the determination of the aflatoxin content in fish. J. Planar Chromatogr.
12, 383-387 (1999). Applicability of OPLC to aflatoxin G2a, B2a, G2, and B2 on silica gel (prewashed with methanol -
acetone 1:1) with chloroform - ethyl acetate - tetrahydrofuran 10:15:1. Quantitative densitometry at 436 nm.
28b
85 080 J. SKARKOVA*, V. OSTRY, (*Nat. Inst. of Public Health, Center for Hygiene and Food Chains, Palackho 1-3, 612 42
Brno. Czech Republic): An HPTLC method for confirmation of the presence of ultra-trace amounts of aflatoxin M1
in human urine. J. Planar Chromatogr. 13, 42-44 (2000). HPTLC of aflatoxin M1 on silica gel with chloroform -
acetone - 2-propanol 17:2:1. Quantitation by densitometry in reflectance mode at 366 nm. Twofold enhancement of the
sensitivity was achieved by immersing the chromatogram plate in a solution of paraffin oil in n-hexane.
28b
85 082 M.A. STANDER, P.S. STEYN*, A. LUEBBEN, A. MILJKOVIC, P.G. MANTLE, G.J. MARAIS, (*School of Chem. and
Biochem., Univ. of Potchefstroom, Potchefstroom 2520, South Africa): Influence of halogen salts on the production
of ochratoxins by Aspergillus ochraceus Wilh. . J. Agric. Food Chem. 48, 1865-1871 (2000). Analytical and
preparative TLC of ochratoxin A, B, (4R)- and (4S)-4-hydroxy-ochratoxin A, (4R)-4-hydroxy-ochratoxin B,
bromo-ochratoxin a, b on silica gel with toluene - acetic acid 4:1. The ochratoxins display a very strong fluorescence
upon UV illumination.
28b
29. Pesticides and other agrochemicals
85 083 T. CSERHATI, E. FORGACS, G. OROS, (Inst. of Chem., Chem. Res. Center, Hungarian Acad. of Sci., P.O. Box 17,

85 083 T. CSERHATI, E. FORGACS, G. OROS, (Inst. of Chem., Chem. Res. Center, Hungarian Acad. of Sci., P.O. Box 17,
1525 Budapest, Hungary): Binding characteristics of a water-soluble b-cyclodextrine polymer. J. Liq.
Chromatogr. 23, 411-421 (2000). Determination of the interaction of 18 commercial pesticides with a water-soluble
b-cyclodextrin polymer by charge-transfer reversed phase TLC and calculation of the relative strength of interaction.
TLC of 18 pesticides (benomyl, carbendazim, STB, thiabendazol, fuberidazol, mebendazol, diethofencab, fenarimol,
nuarimol, prochloraz, myclobutanyl, benalaxyl, furalaxyl, ofurace, metalaxyl, carboxin, Hiram) on RP-18 with water -
methanol mixtures the methanol concentration varying between 30 - 60% in steps of 5%. Development in sandwich
chambers . Visualization after drying at 105°C under UV or by exposure to iodine vapors.
2c, 29
85 084 P. LEWER*, K.L. FINNEY-BRINK, D.O. DUCHELBEIS, (*Dow AgroSciences, 9330 Zionsville Road, Indianapolis,
Indiana 46268, USA): Nature of the residue of [14C]cloransulam-methyl in lactating goats. J. Agric. Food Chem.
48, 2532-2546 (2000). TLC of [14C]cloransulam-methyl residues and metabolites on silica gel with toluene - acetonitrile
- acetic acid 10:9:1 without chamber saturation as mobile phase. [14C] radio scanning. Visualization of non-radiolabeled
reference standards under UV 254 nm and fluorescence quenching.
29
85 085 N.U. PERISIC-JANJIC*, K. POPOV-PERGAL, B. GORDIC, (*Inst. of Chem., Fac. of Sci., Trg Dosijeta Obradovica 3,
21000 Novi Sad, Yugoslavia): Normal- and reversed-phase thin-layer chromatography of some
3-(2'-furoyl)-5-X-2,4-dioxotetrahydro-1,3-thiazole derivatives. J. Planar Chromatogr. 12, 435-439 (1999). HPTLC on
silica gel with hexane - ethyl acetate 4:1 as the best system of the systems investigated. In addition TLC on normal
silica gel, rice starch and cellulose with a variety of different mobile phases. Detection under UV 254 nm.
29f
85 086 P. VASSILEVA-ALEXANDROVA, A. NEICHEVA, (Higher Inst. of Food and Flavour Industry, 4000 Plovdiv, Bulgaria):
Chromatographic study of the residues of fungicidal ethylenebisdithiocarbamates and ethylenethioureas in
plants after oxidative inactivation with potassium permanganate. J. Planar Chromatogr. 12, 425-428 (1996). TLC
of ethylenethiourea on silica gel with either chloroform - butanol - methanol - water 200:10:2:1 or chloroform - ethyl
acetate - methanol 3:2:1. Detection either under UV 254 nm or by spraying with 2% aqueous sodium nitroferricyanide
prepared by dissolving 2 g of Na2Fe(CN)5N ? 2H2O and 4 g of sodium hydroxide in 40 mL of distilled water, addition of
1 g of ammonium peroxodisulfate and dilution to 100 mL with water.
29c
85 087 C. WEINS, (Staatl. Inst. für Gesundheit und Umwelt des Saarlandes, Malstatterstr. 17, 66117 Saarbrücken, Germany):
Bioactivity based analysis in HPTLC/AMD detection of bioactive environmental compounds by
high-performance thin-layer chromatography. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000,
Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 27-35. Summery of bioactivity based analysis in
HPTLC. Examples for enzyme-inhibition tests, antibiotic activity test and tests for fungicidal and herbicidal active
substances in environmental samples. Chromatography is made according to DIN 38407, part 11, i.e. an universal AMD
method for the separation of substances of varying polarity. Identification performed by multi-wavelength scanning at 7
wavelengths in the UV-range. The method is very sensitive (in the ng to pg range).
29, 28a, 37
30. Synthetic and natural dyes
85 088 T. CSERHATI*, E. FORGACS, M. CANDEIAS, L. VILAS-BOLAS, R. BRONCE, J. SPRANGER, (*Inst. of Chem., Chem.
Res. Center, Hungarian Acad. of Sci., P.O. Box 17, 1525 Budapest, Hungaria): Separation and tentative
identification of the main pigment fraction of raisins by thin-layer chromatography - Fourier Transform Infrared
and high-performance liquid chromatography - UV detection. J. Chromatogr. Sci. 38, 145-150 (2000). TLC on
silica gel and alumina impregnated by overnight predevelopment in n-hexane - paraffin oil 19:1, resp. on RP-18.
Development with water containing various concentrations of methanol, acetone, THF and dioxane in sandwich
chambers in the dark; finall development with water - THF 3:7. Evaluation visually and quantification by densitometry at
340 and 370 nm. - TLC - FTIR on-line and off-line coupling.
30b
85 089 J.-F. GONNET*, B. FENET, (*Lab. BMP and Centre de RMN, UCB-ESCPE, UPRESA CNRS 5012, UniversitÈ
Claude-Bernard Lyon I, 43 boulevard du 11 Novembre 1918, F-69622 Villeurbanne, France): "Cyclamen red" colors
based on a macrocyclic anthocyanin in carnation flowers. J. Agric. Food Chem. 48, 22-26 (2000). TLC of e.g.
pelargonidin 3-O-, 3-O-malyl, and 3,5-di-O-glucoside on cellulose with hydrochloric acid - acetic acid - water 3:15:82
and 1-butanol - acetic acid - water 4:1:5, upper phase. Visualization under UV 365 nm.
30b

85 090 D. HORNERO-MENDEZ, M.I. MENGUEZ-MOSQUERA*, (*Departamento de BiotecnologÌa de Alimentos, Instituto de la
Grasa (CSIC), Avenida Padre G·rcia Tejero 4, 41012 Sevilla, Spain): Carotenoid pigments in Rosa mosqueta Hips,
an alternative carotenoid source for foods. J. Agric. Food Chem. 48, 825-828 (2000). TLC of pigments on silica gel
with solvent system A (light petroleum ether 40 608C) for the separation of b-carotene and lycopene, system B
(petroleum ether (65 - 958C - acetone - diethylamine 10:4:1 for e.g. rubixanthin, gazaniaxanthin, b-cryptoxanthin,
zeaxanthin, violaxanthin, antheraxanthin, g-carotene. Detection by observation under white and UV light.
30b
85 091 Gertrud MORLOCK, A. BONHOFF, E. REICH* (*CAMAG, Sonnenmattstr. 11, 4132 Muttenz, Switzerland): Allergien
durch Farbstoffe in Textilien? Allergies through dyes in textiles? GIT Fachz. Lab. Spe. Separation 1, 28-29
(2000). HPTLC of 8 disperse dyes (blue 1, blue 35, blue 106, blue 124, yellow 3, orange 3, orange 37/36, red 1) on
silica gel with chloroform - ethyl acetate - n-hexane 6:3:2, bandwise application. Mixtures with coeluting dyes can be
investigated using the AMD technique with multiple development (in 20 steps) with a gradient from methanol via
dichloromethane to n-hexane. Quantification by densitometry at 254 nm.
30a
85 092 J.K. ROZYLO, R. SIEMBIDA, (Maria Curie-Sklodowska University, Fac. of Chem., Maria Curie-Sklodowska Sq. 3,
20031 Lublin, Poland): OPLC analysis of food dyestuffs. Chem. Environ. Res. 6, 49-55 (1997). OPLC of quinoline
yellow (E104), sunset yellow (E110), cochineal red A (E124), indigo carmine (E132), tartrazine (E102), amaranth
(E123), erythrozine (E127) on silica gel with NH3 - methanol - ethyl acetate 1:3:6 and NH3 - MEK - n-butanol 2:3:5 to
80 mm. Limits of detection are below 0,5 µg. E127 migrates in both solvent systems into the demixing zone. Thus, it
could not be determined quantitatively. Significantly decrease in time of chromatographic analysis as compared to
conventional TLC.
30a
31. Plastics and their intermediates
85 093 F. FIALA*, I. STEINER, K. KUBESH, (*Consumer Council, Austrian Standards Inst., A-1021 Vienna, Austria):
Migration of di-(2-ethylhexyl)phthalate (DEHP) and diisononylphthalate (DINP) from PVC articles. Dtsch.
Lebensm. Rundsch. 90, 51-57 (2000). HPTLC of DEHP and DINP on silica gel RP-18 w with acetone - water - ethanol
3:1:1. Quantitative evaluation by densitometry at 254 nm. The method requires low amounts of solvents, is very fast (10
min for 10 samples on one plate ) and is as sensitive as HPLC.
31
32. Pharmaceutical and biomedical applications
85 094 D. AGBABA, S. ERIC, G. MARKOVIC, V. NEDELJKOVIC, S. VESELINOVIC, M. VUCETIC, (School of Pharm., Dept. of
Pharm. Chem., P.O. Box 146, 11221 Belgrade and Galenska Lab., Zajecar, Yugoslavia): The application of HPTLC in
the quantitative analysis of drugs. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary,
24-26 June 2000, Res. Inst. for Med. Plants, p. 143-148. Validated methods: HPTLC of dexamethasone sodium
phosphate and neomycin sulfate on silica gel with concentration zone with chloroform - 1-propanol - NH3 1:3:2. HPTLC
of ketoconazole on silica gel with n-hexane - acetone 4:1. HPTLC of coenzyme Q10 on silica gel with n-hexane -
chloroform - methanol 5:4:1. Quantitation by polynomial regression at 246 nm for dexamethasone sodium phosphate, at
230 nm for ketoconazole, at 275 nm for coenzyme Q10 and after derivatization with ninhydrin solution at 500 nm for
neomycin. Precision is found to be between 0,8-2,68 %.
32a, 28
85 095 A.P. ARGEKAR*, S.G. POWAR, (*Anal. Lab., Dept. of Chem., The Inst. of Sci., 15 Madam Cama Road, Mumbai
400032, India): Simultaneous determination of diloxanide furoate and tinidazole in tablets by high-performance
thin-layer chromatography. J. Planar Chromatogr. 12, 452-455 (1999). HPTLC of diloxanide furoate and tinidazole
on silica gel with dichloromethane - methanol 192:1. Quantitation by densitometry at 280 nm. RSD 1.1% and 1.3% for
diloxanide furoate and tinidazole, respectively. New simple, sensitive, rapid, and stability indicating HPTLC method.
32a
85 096 A.P. ARGEKAR*, J.G. SAWANT, (*Anal. Lab., Dept. of Chem., The Inst. of Sci., 15 Madam Cama Road,
Mumbai-400032, India): Simultaneous determination of paracetamol and mefenamic acid in tablets by HPTLC. J.
Planar Chromatogr. 12, 361-364 (1999). HPTLC of paracetamol and mefenamic acid on silica gel with toluene -
acetone - methanol 8:1:1. Detection and quantification by densitometry at 263 nm. The linearity ranges were 120-360
ng/µl; RSD between 0.41 and 0.78%; recoveries were in the range of 95.50-103.60%. New simple, precise, accurate
and rapid HPTLC method.
32a

85 097 H.M. BASEKI, J. SHERMA*, (*Dept. of Chem., Lafayette Coll., Easton, PA 18042, USA): Quantification of triprolidine
hydrochloride and methscopolamine nitrate in pharmaceutical tablets by HPTLC with ultraviolet absorption
densitometry. J. Planar Chromatogr. 13, 16-19 (2000). TLC of triprolidine hydrochloride and methscopolamine nitrate
using automated band application on silica gel plates with fluorescent indicator, and scanning densitometry of
fluorescence-quenched zones of samples and standards. HPTLC on silica gel with ethyl acetate - methanol - conc. NH3
17:2:3 to a distance of 6 cm for triprolidine hydrochloride and dichloromethane - methanol - 1-butanol - acetic acid -
deionized water 25:10:15:15:2 for methscopolamine nitrate. Densitometry at 267 nm resp. 205 nm. Precision was
0.75% resp 1.4 5 RSD, recovery from spiked samples averaged 98.6% resp 99.4%. New quantitative method with
excellent accuracy and precision, higher selectivity than spectrometry and less complex and faster than HPLC.
32, 22
85 098 Y. CHEN (Chen Yanqing), (Sichuan Guangda Pharm. Co. Ltd., Pengzhou, Sichuan 61930, P.R. China): (The quality
standard of Sauhuang capsules. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (2), 125-128
(2000). TLC on silica gel with 1) ethyl acetate - butanone - formic acid - water 5:3:1:1, 2) petroleum ether (30-60°C) -
ethyl formate - formic acid 15:5:1. Detection 1) under UV 365 nm, 2) by spaying with 2% FeCl3 in ethanol, 3) by
exposure to ammonia vapors. Identification by finger print technique. Quantitation of berberine chloride by densitometry
at 425 nm. Discussion of use of the method for the quality control of the medicine.
32g
85 099 S. DENG (Deng Suynan)*, J. HE (He Jiajun), Q. CHEN (Chen Qiaokug), (*Guangzhou Baiyunshan Pharm. Co. Ltd.,
Guangzhou 510515, P.R. China): (The quality standards of Jinlinkang oral liquid. ) (Chinese). J. Chinese Trad.
Patent Med. (Zhongchengyao) 22 (5), 335-337 (2000). TLC on silica gel with 1) cyclohexane - chloroform - ethyl
acetate - acetic acid 40:10:16:1, 2) chloroform - methanol - water 13:7:2, 3) chloroform - methanol 9:1. Detection by
spraying with 10% sulfuric acid in ethanol and heating at 105°C for 10 min and under UV 365 nm. Identification by
finger print technique. Quantitation of ursolic acid by densitometry at 520 nm. Discussion of the use of the method for
the quality control of the medicine.
32g
85 100 S. ERIC, D. AGBABA*, S. VLADIMIROV, D. ZIVANOV-STAKIC, (*Dept. of Pharm. Chem., Fac. of Pharmacy, Vojvode
Stepe 150, P.O. Box 146, 11000 Belgrade, Serbia, Yugoslavia): The retention behavior of several semisynthetic
cephalosporins in planar chromatography. J. Planar Chromatogr. 13, 88-92 (2000). TLC of cephalosporins
(cephalexine, cefaclor, ceftriaxone, cefixime, cefotaxime) on silica gel developed with a variety of binary mobile phases
containing different amounts of organic modifier. The influence of type, chemical character, and position of the
substitution of functional groups (steric and structural effects) on the retention was of particular interest.
32a, 2e
85 101 Y. FENG (Feng Yanming)*, X. JIAG (Jiang Xinyuan), (*Guizhou Pharm. Fact., Guiyang 550004, P.R. China):
(Identification of Handanganla capsule by thin-layer chromatography. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 22 (3), 201-202 (2000). TLC on silica gel with 1) benzene - chloroform - acetone 10:8:1, 2) chloroform
- acetone - methanol - conc. NH3 125:25:20:1, 3) ethyl acetate - butanone - methanol - water 5:3:1:1. Detection 1)
under daylight, 2) by spraying with potassium iodobismuthate (5% in water), 3) by spraying with 10% sulfuric acid in
ethanol and heating at 105°C for 5 min. Identification by finger print.
32g
85 102 M. FENSKE, (Dept. Animal Physiol., NW1, Univ. Bayreuth, DE-95440 Bayreuth, Germany): Rapid and specific
determination of free cortisol in Guinea pig urine and faeces by thin-layer chromatography - competitive protein
binding assay. Chromatographia 50 (1/8), 428-432 (2000). TLC on silica gel with acetone - toluene 1:1. Quantitation
by densitometry at 254 nm. Comparison with previous methods, stressing on TLC's merits like the use of
dexamethasone as a marker for cortisol localization on TLC sheets, the use of buffer instead of organic solvents to
recover cortisol form silica gel and the rapidity of the competitive protein-binding assay (1 - 2 h).
32f
85 103 K. GLOWNIAK*, T. WAWRZYNOWICZ, M. HAJNOS, T. MROCZEK, (*Dept. of Pharmacognosy, Med. Univ., Peowiaków
12, 20-007 Lublin, Poland): The application of zonal thin-layer chromatography to the determination of paclitaxel
and 10-deacetylbaccatin III in some Taxus species. J. Planar Chromatogr. 12, 328-335 (1999). Optimization of a
chromatographic procedure. HPTLC and TLC of paclitaxel (taxol) and 10-deacetylbaccatin III on silica gel using a
multicomponent mobile phase like chloroform - benzene - acetone - methanol 34:8:6:3. Visualization under UV 254 nm
and by spraying with Krowitsky reagent (0.5% CeSO4 + 1% phosphomolybdic acid in 10% sulfuric acid). Evaluation by
densitometry at 232 nm.
32e
85 104 L. GU (Gu Lihong)*, P. ZHU (Zhu Pinye), (*Guangzhou Inst. Drug Cont., Guangzhou 510160, P.R. China):

85 104 L. GU (Gu Lihong)*, P. ZHU (Zhu Pinye), (*Guangzhou Inst. Drug Cont., Guangzhou 510160, P.R. China):
(Determination of andrographolide and 14-deoxy-11,12-didehydroandrographolide in Andrographics paniculata
by thin-layer chromatography. ) (Chinese). J. Chinese Herb Med. (Zhongcaoyao) 31 (4), 260-262 (2000). TLC on
silica gel with chloroform - acetone 2:1 and chloroform - ethyl acetate - methanol 20:15:2. Detection under UV.
32g
85 105 X. HONG (Hong Xiaoqun)*, ZH. WANG (Wang Zhihua), Q. LI (Li Qingun), Y. ZOU (Zou Yun), (*Shanghai Univ. Chin.
Trad. Med. & Pharm., Shanghai 200032, P.R. China): (Analysis and testing of Chinese traditional medicine and
patent medicine. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (1), 80-100 (2000). A review with
728 references on the title topic, involving 270 references of TLC, 212 of HPLC, 139 of spectroscopy, 63 of GC and
some others.
32
85 106 I. HOPIRTEAN, A. BOTA, I. HOPIRTEAN, C. MARUTOUI*, (*Inst. of Chem. 'Raluca Ripan', 30 Fântânele Street,
RO-3400 Cluj-Napoca, Romania): The use of TLC in the analysis of oxazepam. J. Planar Chromatogr. 12, 392-394
(1999). TLC of seven intermediates from all steps of the syntheses of oxazepam on silica gel. After cleaning the plate
by successive development with chloroform - ethyl acetate 3:1 and aqueous NH3 - methanol 1:9, chromatography with
chloroform - acetone and chloroform - ethyl acetate mixtures in different ratios in previously saturated N-chambers. The
optimum chromatographic system for related compounds was found to be chloroform - ethyl acetate 3:1. Detection
under UV 254nm.
32a
85 107 A. JAMSHIDI, M. ADJAVADI, S. SHAHMIRI*, A. MASSOUMI, (*Dept. of Chem., Sci. and Res. Branch, Islamic Azad
Univ., P.O. Box 14515/775, Tehran, Iran): HPTLC assay for ibuprofen in newly formulated liquorice containing
tablets. J. Planar Chromatogr. 13, 45-47 (2000). HPTLC of ibuprofen on silica gel with toluene - acetone - formic acid
35:14:1. Quantitative evaluation by measurement of fluorescence quenched zones by densitometry at 230 nm
(absorbance).
32a
85 108 A. JAMSHIDI, S. SHAHMIRI, M. ADJVADI, (Iran Polymer Institute, P.O. Box 14965/115, Tehran and Dept. of Chem.,
North Tehran Branch, Islamic Azad Univ., 213/C Saman Bldg., Vanak 14358 Tehran and Darou Pakhsh Pharm.
Research Center, P.O. Box 13185/877, Tehran, Iran): HPTLC assay for aspirin and free salicylic acid in newly
formulated liquorice-containing tablets. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred,
Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 231-235. Validated HPTLC on silica gel with ethyl acetate -
methanol - acetic acid 80:19:1 for ca. 15 min. Quantitation by absorbance measurement at 300 nm (polynomial
regression). Recovery was found to be between 99,0 and 101,3 %, repeatability was 0,1 % for aspirin and 2,2% for
salicylic acid (n=2). HPTLC method is preferred to HPLC method because of its speed (14 samples in 30 min).
32a, 11a, 2f
85 109 A. KASSAI, A. SZECSI, A. KOPPANY, Z. VEGH, K. FERENCZI-FODOR*, (*Gedeon Richter Ltd., H-1475 Budapest 10,
P.O. Box 27, Hungary): Analytical transfer of an OPLC purity test and application for in-process monitoring. J.
Planar Chromatogr. 13, 30-32 (2000). OPLC of steroid drugs and related substances on silica gel with cyclohexane -
ethyl acetate - chloroform 6:2:2. Visualization by spraying with a solution of 10% ethanolic sulfuric acid and heating at
120°C for 2 min. Evaluation of chromatograms was performed by visual inspection under UV 366 nm.
32a
85 110 CH. KONG (Kong Chun)*, L. WEN (Wen Liyu), ZH. DING (Ding Zhongbo), (*Yangzhou Municip. Inst. Drug Cont.,
Yangzhou 225009, P.R. China): (Separation and identification of flavonoid compositions with micro-emulsion
thin-layer chromatography. ) (Chinese). Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 20 (2), 121-124 (2000). TLC of
14 kinds of flavonoid drugs on polyamide film with 6 kinds of SDS / butanol - hexane - water micro-emulsions as the
mobile phase. Detection by spraying with 1% AlCl3 in ethanol and under UV 365 nm. Identification of the flavonoid
compositions in rutin, quercetin and scutellarin by fingerprint. Comparison of the results with those obtained with normal
mobile phase, showing advantages of present method. Optimal mobile phase was a micro-emulsion containing 75%
water - formic acid 19:1.
32c, 8a
85 111 J. KRZEK, M. STAREK, (Dept. of Inorg. and Anal. Chem., Coll. Med. of Jagiellonian Univ., Medyczna 9, 30-688

85 111 J. KRZEK, M. STAREK, (Dept. of Inorg. and Anal. Chem., Coll. Med. of Jagiellonian Univ., Medyczna 9, 30-688
Kraków, Poland): Densitometric determination of active constituents and impurities in complex analgesic and
antipyretic pharmaceuticals. J. Planar Chromatogr. 12, 356-360 (1999). TLC of acetylsalicylic acid, paracetamol,
caffeine and salicylic acid on silica gel with n-hexane - chloroform - acetic acid 4:1:1 or ethanol - n-hexane - chloroform -
acetic acid 12:12:8:1 for determination of ascorbic acid, and chloroform - acetone - toluene 12:5:2 for determination of
paracetamol in the presence of ascorbic acid. Quantitation by densitometry at 280 nm. Rapid and accurate identification
and quantitative determination.
32a
85 112 D. LAI (Lai Dongmei)*, Y. YANG (Yang Ying), (*Shamen Municip. Inst. Drug Cont., Shamen 361012, P.R. China):
(Determination of cholic acid in compound calcalas boins pulvis by thin-layer chromatography. ) (Chinese). J.
Chinese Trad. Patent Med. (Zhongchengyao) 22 (3), 238-239 (2000). TLC on silica gel with hexane - ethyl acetate -
acetic acid - methanol 15:35:2:4. Detection by spraying with 10% sulfuric acid in ethanol and heating at 100°C for
10min. Quantitation by densitometry at 390 nm.
32c
85 113 R. LI (Li Ruilian)*, J. SU (Su Jianjun), (*Hunan Inst. Drug Cont., Changsha 410001, P.R. China): (Determination of
matrine in Shikaugfu granules II by thin-layer chromatography. ) (Chinese). J. Chinese Herb Med. (Zhongcaoyao)
31 (1), 16-18 (2000). TLC on silica gel with toluene - acetone - ethanol - conc. NH3 20:20:3:1. Detection by spraying
with potassium iodobismuthate reagent. Identification by comparison of the Rf and in situ spectra with the standard.
32g
85 114 T. LIN (Lin Tong), (Guangzou Municip. Inst. Drug Cont., Guangzhou 510160, P.R. China): (Determination of cholic
acid in Xiaozhiling capsules by thin-layer chromatography. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 22 (5), 379-380 (2000). TLC on silica gel with hexane - ethyl acetate - acetic acid -methanol
20:25:2:3. Detection by spraying with 10% sulfuric acid in ethanol and heating at 100°C for 5 min. Quantitation by
densitometry at 385 nm. Discussion of using the procedure for the quality control of the medicine.
32g
85 115 J. LIU (Liu Jijin), (Shenzhen Municip. Inst. Drug Cont., Shenzhen 51029, P.R. China): (Determination of chlorogenic
acid in Xuandong infusion by thin-layer chromatography. ) (Chinese). Chinese J. Pharm. Anal. (Yaowu Fenxi
Zazhi) 20 (2), 131-132 (2000). TLC on silica gel with the upper phase of butyl acetete - formic acid - water 14:5:5.
Detection under UV 365 nm. Quantitation by densitometry at 320 nm. Identification by comparison with the standard.
32g
85 116 Q. LIU (Liu Qianguang)*, Z. ZHANG (Zhang Zunting), KURBANJIANG, Zh. CHEN (Chen Zhanguo), (*Dept. Chem.,
Shanxi Univ. Norm., Xian 710062, P.R. China): (Determination of emodin in Binlang Sixiao pills by thin-layer
chromatography. ) (Chinese). Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 20 (1), 63-64 (2000). TLC on silica gel
with toluene - ethyl acetate - formic acid 15:2:1. Detection under UV 365 nm. Identification by comparison with the
standard. Quantitation by densitometry at 440 nm.
32g
85 117 Z. LIU (Liu Zuoping)*, Y. LIANG (Liang Yangqun), L. XU (Xu Liqin), (*Wuhan Inst. Drug Cont., Wuhan 430012, P.R.
China): (Study of the quality standards for Weitong plaster. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 22 (6), 407-409 (2000). TLC of the extracts of the title Chinese traditional patent medicine on silica
gel with 1) hexane - ethyl acetate 18:3, 2) chloroform - acetone - formic acid 140: 60:1, 3) benzene - ethyl acetate 6:1.
Detection under UV 365 nm and by spraying with vanillin-sulfuric acid reagent followed by heating at 100°C for 5 min.
Identification by finger print. Quantitation of eugenol by GC. Discussion of the TLC procedures combined with GC as the
quality control method for the medicine.
32g
85 118 D. MATOSIUK*, K JOZWIAK, (*Dept. of Synth. and Techn. of Drugs, Fac. of Pharm., Med. Univ., Staszica 6, 20-081
Lublin, Poland): Determination of the lipophilicity of carbonyl derivatives of 2-aminoimidazolines-2 by
reversed-phase thin-layer chromatography. Part 1. Lipophilicity of 1-(1-arylimidazolin-2-yl)-3-arylureas and
1-aryl-2-imine-3-arylaminocarbonylimidazolidines. J. Planar Chromatogr. 13, 52-56 (2000). Determination of the
lipophilicity of forty new derivatives of 1-aryl-2-aminoimidazolines (twenty 1-(1-arylimidazolin-2-yl)-3-arylureas and
twenty 1-aryl-1-2-imine-3-arylaminocarbonyl-imidazolidines) by RP-TLC with methanol - acetic acid - water. Visualization
under UV at 254 nm.
32a
85 119 S. MENDOZA MARIN, V.H. JARAMILLO MENA, M. VEGA HERRERA, (Univ. de Concepcion, Fac. de Farmacia,

85 119 S. MENDOZA MARIN, V.H. JARAMILLO MENA, M. VEGA HERRERA, (Univ. de Concepcion, Fac. de Farmacia,
Casilla 237, Concepcion, Chile): (HPTLC-determination of metamizol-Na in suppositories). (Spanish). Bol. Soc.
Chil. Quim. 44, 479-485 (1999). HPTLC validation of metamizol-Na on silica gel with ethyl acetate - methanol - NH3
(75:24:1) to 50 mm. Quantitation by densitometry at 273 nm by linear regression (50-250 ng). Limit of detection is 10,9
ng and limit of quantification 16,1 ng. CV is 2,45%, recovery is 102,48% (225 ng), 98,78% (250 ng) and 100,69% (275
ng). The HPTLC method is precise, exact, proportional, linear and sensitive. Results do not differ statistically from the
Official Method described in the German Pharmacopoeia.
32a
85 120 F.J. MORENO, A. OLANO, G. SANTA-MARIA, M. CORZO*, (*Inst. Ferment. Inst., CSIC, c/Juan de la Cierva 3, 28006
Madrid, Spain): Determination of mallodextrins in enteral formulations by three different chromatographic
methods. Chromatographia 50 (11/12), 705-709 (2000). TLC on silica gel with acetonitrile - ethyl acetate - propanol -
water 17:4:10:10. Detection by dipping into an ethanolic solution of a-naphthol (0.5% m/v) and sulfuric acid 5% and
heating at 110°C for 10 min. Quantitation by densitometry.
32f
85 121 D. NIE (Nie Dongdong), (Jixi Municip. Inst. Drug Cont., Jixi, Helonjiang 159100, P.R. China): (The quality standard for
compound Tanshinon tablets. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (4), 267-268 (2000).
TLC of notoginsenoside R1 on silica with chloroform - methanol - water 13:7:2. Detection by spraying with 10% sulfuric
acid in ethanol 3:7:2. Detection by spraying with 10% sulfuric acid in ethanol and heating at 1108C for 5 min and under
UV 365 nm. Discussion of the effects of sample preparation, developing system, temperature and moisture on the
results. The improved method can be used to control the quality of the medicine.
32g
85 122 N.U. PERISIC-JANJIC*, S.O. PODUNAVAC-KUZMANOVIC, J.S. BALAZ, D. VLAOVIC, (*Inst. of Chem., Fac. of Sci.,
Univ. of Novi Sad, Trg. D. Obradovica 3, 21000 Novi Sad, Yugoslavia): Chromatographic behaviour and lipophilicity
of some benzimidazole derivatives. J. Planar Chromatogr. 13, 123-129 (2000). TLC of
2-amino-5,6-dimethylbenzimidazoles, 2-aminobenzimidazoles, and 5,6-dimethylbenzimidazoles on layers of rice starch,
cellulose and Aminoplast, a carbamide-formaldehyde polymer, with conc. NH3 - n-propanol in varying compositions for
rice starch and cellulose resp. cyclohexane - acetone - conc. NH3 in varying concentrations for Aminoplast. Dark spots
were observed under UV 254 nm.
32a
85 123 D.S. RATHOD, B.D. MALI, M.V. GARAD, V.B. PATIL*, (*Reg. Forensic Sci. Lab., State of Maharashtra, Cantonment,
Aurangabad-431 002, India): Thin-layer chromatographic detection of denatonium saccharide (Amerex) in
denatured alcohol. J. Planar Chromatogr. 13, 146-148 (2000). TLC of the bitterant denatonium saccharide on silica
gel with ethanol - methanol - butanol - hydrochloric acid 18:48:18:0.05 in a presaturated chamber. Detection after drying
by exposure to chlorine gas for ca 5 min. After removal of all excess chlorine, the plate was uniformly sprayed with
o-tolidine reagent and then with 1% phosphomolybdic acid. The detection limit for denatonium saccharide is 0.2 µg per
spot.
32d
85 124 E. REICH (CAMAG, Sonnenmattstr. 11, 4132 Muttenz, Switzerland): Qualitative and quantitative analysis of
herbals and herbal medicinal products (HMP) by HPTLC. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography
2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 45-46. HPTLC is extremely well suited for
analysis of herbal preparations, i.e. identity check, quality control of raw materials and finished products as well as
stability tests and determination of marker substances. The complete paper will be published in one of the forthcoming
issues of J. Planar Chromatogr.
32g
85 125 SH. SUN (Sun Shikang), (Tianshui Municip. Inst. Drug Cont., Tianshui Gansu 741018, P.R. China): (The quality
standard for Fructus chebulae granules. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (6)
409-411 (2000). TLC on silica gel with chloroform - methanol - ethyl acetate - water 90:7:5:2. Detection by spraying with
2% AlCl3 in ethanol under UV 365 nm. Identification by finger print. Quantitation of total flavonoids by
spectrophotometry. Discussion of using the method for the quality control of the patent medicine.
32g, 8
85 126 B. WANG (Wang Baoqing)*, F. ZHOU (Zhou Furong), (*Nat. Inst. For Cont. Pharm. & Biolog. Prod., Beijing 100050,
P.R. China): (Standardization of Chinese materia medica in retrospect. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 20 (1), 22-32 (2000). A review with 65 references involving testing methods, TLC as well as GC and
HPLC. Stressing on the advantages, such as suitability for complex medicine, simpleness of sample preparation,
flexibility in detection, etc.
32

85 127 R. WANG (Wang Renying), (Shenlu Company Group, Hefei, Anhui 230041, P.R. China): (Determination of emodin in
Huazhouqingshen granules by thin-layer chromatography. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 22 (4), 269-270 (2000). TLC on silica gel with benzene - ethyl acetate - acetic acid 150:50:3.
Quantitation by densitometry at 445 nm. Discussion of the procedure for sample preparation and the elimination of the
interference of some co-components.
32g
85 128 SH. WANG (Wang Shouyu), (Tianjin Cent. Hosp., 2nd, Tianjin 300120, P.R. China): (Quality control of Xinhua oral
liquid by thin-layer chromatography. ) (Chinese). J. Chinese Herb Med. (Zhongcaoyao) 31 (3), 189-190, 224 (2000).
TLC on silica gel with 1) chloroform - ethyl acetate - methanol 45:5:3, 2) chloroform - ethyl acetate - methanol 45:4:2, 3)
petroleum ether (60-90°C) - ethyl acetate 2:1. Detection under UV 365 nm. Identification by finger print (Rf and color).
32g
85 129 E. WESTGATE, J. SHERMA, (Dept. of Chem., Lafayette Coll., Easton, PA 18042, USA): Determination of the
sunscreen oxybenzone in lotions by reversed-phase HPTLC with ultraviolet absorption densitometry. J. Liqu.
Chroatogr. 23, 609-615 (2000). New procedure HPTLC of oxybenzone (2-hydroxy-4-methoxy-benzophenone) on RP-18
(after prewashing with dichloromethane - methanol 1:1) with methanol - tetrahydrofuran - deionized water 10:7:3 in a
paper-lined chamber. Quantitation by densitometry at 300 nm. Precision (RSD) was 2.2% for replicate analysis of four
sample solutions of one lotion on one plate and 4.6% for four analyses of one sample solution on different plates. Errors
from spiked blanks and standard addition analyses to validate accuracy were 0 and 0.67% for the two blanks and 1.3%
for the standard addition validation.
32a
85 130 P. XIE (Xie Peishan), (Guangzhou Inst. Drug Cont., Guangzhou 510160, P.R. China): (Identification of Chinese
patent medicine by using chromatographic finger print technique. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 22 (6), 391-395 (2000). Discussion of the evaluation of the quality standards and control of the
Chinese traditional patent medicines, taking chromatographic finger print as the feasible technique, stressing on the
standardization of the materials used and the procedures taken. Demonstration of a few fingerprints obtained by TLC,
HPLC and GC.
32g
85 131 P. XIE (Xie Peide)*, H. ZHANG (Zhang Hua), ZH. ZHANG (Zhang Zhijun, (*Guangxi Antonormous Region Inst. Drug
Center, Nannin 530021, P.R. China): (Study of the quality standard of compound Liangmianzhen tablets. )
(Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (5), 333-335 (2000). TLC on silica gel with 1) benzene -
ethyl acetate 19:1, 2) chloroform - methanol conc. NH3 200:5:1. Detection 1) by spraying with phosphomolybdic acid in
ethanol - chloroform 1:1 (1%) and heating at 105°C for 10 min, 2) by spraying with diluted potassium iodobismuthate
and under UV 365 nm. Identification by finger print technique. Quantitation of menthol by GC. Discussion of the use of
the method for the quality control of the medicine.
32g
85 132 A. YAMASHITA, Y. KAMANO*, T. NOGAWA, H. HIRAIDE, T. KAZAMA, M. TOZAWA, C. L. HERALD, G.R. PETTIT,
(*Fac. of Sci., Kanagawa University, 2946 Tuchiya, Hiratsuka, Kanagawa 259-1293, Japan): Thin-layer
chromatography of bryostatins. J. Planar Chromatogr. 12, 336-339 (1999). TLC of 11 bryostatins on silica gel with
n-hexane - acetone (7:3) and n-hexane - ethyl acetate (1:1) and with methanol - water 3:1 for reversed phase
chromatography ( silica gel RP-18) as mobile phases in single, multiple or two-dimensional developments. The method
was also used in support of structure elucidation. Detection under UV 254 nm and by color reaction after spraying with
anisaldehyde- sulfuric acid reagent and heating at approx. 140°C.
32e
85 133 ZH. YANG (Yang Zhenxia)*, L. WANG (Wang Lixia), B. ZHANG /Zhang Bochong), (*Gansu Provin. Inst. Drug Cont.,
Lanzhou 730000, P.R. China): (Determination of ginsenoside Rg1 in Shujinlijie liniment film by thin-layer
chromatography. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (2), 128-130 (2000). TLC on silica
gel with the upper phase of butanol - ethyl acetate - water 4:1:5. Detection by spraying with 5% sulfuric acid in ethanol
and heating at 105°C for 5 min. Quantitation by densitometry at 525 nm.
32g
85 134 D. ZHANG (Zhang Dianrui)*, ZH. ZHANG (Zhang Zhaowang, J. SUN (Sun Irmei), (*Shandong Univ. Med. Sci., Jinan

85 134 D. ZHANG (Zhang Dianrui)*, ZH. ZHANG (Zhang Zhaowang, J. SUN (Sun Irmei), (*Shandong Univ. Med. Sci., Jinan
250012, P.R. China): (The quality standard of Sishen Cha Syrup. ) (Chinese). J. Chinese Herb Med. (Zhongcaoyao)
31 (1), 21-23 (2000).TLC on silica gel with 1) petroleum ether (60-90°C) - benzene 1:1, 2) benzene -ethyl acetate 19:1,
3) petroleum ether (60-90°C) - chloroform - methanol 8:48:1, 4) petroleum ether (60-90°C) - ethyl acetate 3:1. Detection
1) by spraying with 10% sulfuric acid in ethanol and heating at 105°C for 5 min, 2) under UV 254 nm and / or 365 nm.
Identification by finger print. Quantitation of psoralen by densitometry at 310 nm. Discussion of the quality control of the
medicine using the method.
32g
85 135 L. ZHANG (Zhang Ling)*, J. ZHAO (Zhao Jianping), (*Shanxi Inst. TCM, Taiyuan 030012, P.R. China): (The quality
standard of Sanhuang inhibin-hyperfunction capsule. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao)
22 (6), 413-445 (2000). TLC on silica with 1) butanol - acetic acid - water 7:1:2, 2) cyclohexane - ethyl acetate - acetic
acid 36:12:1, 3) chloroform - methanol 5:1. Detection under UV 365 nm, by spraying with 5% FeCl3 in ethanol, and by
spraying with 1% vanillin in sulfuric acid and heating for 10 min. Identification by finger print. Quantitation of
astragaloside IV by densitometry at 520 nm. Discussion of using the method for the quality control of the medicine.
32g
85 136 Y. ZHANG (Zhang Ying)*, L. LIN (Lin Linan), Y. ZHAO (Zhao Yanqig), (*Qilu Pharm. Fact., Jinan 250100, P.R. China):
(Study of the extraction technology of Ramulus uncariae cum uncis. ) (Chinese). J. Chinese Trad. Patent Med.
(Zhongchengyao) 22 (5), 331-332 (2000). TLC of rhynchophylline on silica gel with toluene - acetone - ethanol - conc.
NH3 20:25:5:3. Detection by spraying with diluted potassium iodobismuthate - 1% FeCl3 in ethanol 2:1. Quantitation by
densitometry. Investigation of the influence of different extraction conditions on the active components extracted, which
was optimized to be 70% ethanol.
32e
85 137 R. ZHONG (Zhong Raijian)*, J. YE (Ye Jinzhi), (*Jiangxi Inst. Drug Cont., Nanchang 330046, P.R. China): (The quality
standard for Neiyankang granules. ) (Chinese). J. Chinese Herb Med. (Zhongcaoyao) 31 (2), 104-105 (2000). TLC
on silica gel with 1) toluene - ethyl acetate - formic acid 5:4:4, 2) petroleum ether (60-90°C) - ethyl acetate 17:3, 3)
benzene - ethyl acetate - methanol - iso-propanol - water 21:5:5:10. Detection 1) by spraying with 2% FeCl3 in ethanol,
2) by spraying with 2% dinitrophenylhydrazine in ethanol, 3) under UV 365 nm. Identification by fingerprint. Quantitation
of berberine chloride by densitometry at 366 nm. Discussion of the quality control of the medicine with these
procedures.
32g
85 138 ZH. ZHOU (Zhou Zhenying)*, X. WU (Wu Xiao), (*Sichuan Enwei Inst. Pharm., Chengdu 010041, P.R. China):
(Determination of tetrahydropalmatine in Tiaojingzhitong capsules by thin-layer chromatography. ) (Chinese).
J. Chinese Trad. Patent Med. (Zhongchengyao) 22 (3), 194-195 (2000). TLC on silica gel with ether - cyclohexane -
methanol 10:6.1. Detection by spraying with potassium iodobismuthate (5%) in water. Quantitation by densitometry at
510 nm.
32g
33. Inorganic substances
85 139 V. GHOULIPOUR, S.W. HUSAIN*, (*Fac. of Chem., Univ. of Tarbiat Moallem, 49 Mofatteh Avenue, Tehran.15614 ,
Iran): New sorbents in planar chromatography: I. Separation of inorganic ions on layered double hydroxides. J.
Planar Chromatogr. 12, 378-382 (1999). TLC of 26 inorganic cations and 17 anions on LDH (layered double
hydroxides) layers developed with 32 mobile phases. LDH layers were prepared by addition of magnesium or
aluminium nitrate solutions to a solution of sodium hydroxide and sodium carbonate in water. Detection by spraying
with 14 different detection reagents. Separations of one ion from several other ions as well as ternary and binary
separations have been developed.
33
85 140 A. MOHAMMAD*, J.P.S. CHAHAR, E. IRAQI, V. AGRAWAL, (*Anal. Lab., Dept. of Applied Chem., Z. H. Coll. of Eng.,
Aligarh Muslim Univ., Aligarh-202002, India): A new chromatographic iodometric method for the separation and
determination of iodide and oxyanions. J. Planar Chromatogr. 13, 12-15 (2000). Examination of the retention
behavior of iodide, iodate and periodate on silica gel, cellulose, alumina and kieselguhr with mixtures of 0.1 M NH3 and
acetone as mobile phase. Alumina and 0.1 M NH3 - acetone 9:1 was best because it furnished more compact and
well-resolved spots of the anions.
33b
85 141 A. PATRUT, C. MARUTOIU*, O.-F. MARUTOIU, M. I. MOISE, (*Inst. of Chem. 'Raluca Ripan', 30 F‚nt‚nele Street,

85 141 A. PATRUT, C. MARUTOIU*, O.-F. MARUTOIU, M. I. MOISE, (*Inst. of Chem. 'Raluca Ripan', 30 F‚nt‚nele Street,
Ro-3400 Cluj-Napoca, Romania): A new visualization reagent for cation separation by TLC. J. Planar
Chromatogr. 13, 151-153 (2000). TLC separation of Cr, Mn, Fe, Co, Ni, Cu, Zn, Ag, Cd, Pb on cellulose with methanol
- benzene - ethyl methyl ketone - 1-butanol - 25% NH3 6:4:4:3:3, methanol - benzene - ethyl acetate - ethyl methyl
ketone - 25% NH3 5:4:5:3:3, and methanol - benzene - ethyl methyl ketone - 1-butanol - 2 M HCl 6:4:4:3:3. Detection
after drying by spraying with a new visualization reagent, a 0.01% solution of 9-formylacridine in dichloromethane. The
plates were then heated at 120°C for 10 min and examined under UV 366 nm.
33a, 3e
85 142 S.D. SHARMA, S.C. SHARMA, C. SHARMA, (Anal. Res. Lab., Dept. of Chem., Hindu Coll., B3, Jigar Colony, Morabad
244001, India): Reversed-phase thin-layer chromatography of some d-block metal ions on unconventional
supports in complexing acid media. J. Planar Chromatogr. 12, 440-445 (1999). TLC of 20 metal ions on starch,
alumina, starch-alumina 1:1, starch - talc 1:1, silica gel - talc 1:1 and alumina - talc 1:1 with oxalic acid, tartaric acid,
citric acid, and succinic acid, 0.1 M each, as mobile phase. Admixture of talc with starch proved very useful in effecting
a number of analytically important metal-ion separations.
33a
85 143 S.D. SHARMA *, C. SHARMA, (*Anal. Res. Lab., Dept. of Chem., Hindu Coll., B-3, Jigar Colony, Morabad-24001,
India): Retention behavior of some d- and f-block metal ions on untreated and tri-n-butylamine-impregnated
silica gel G layers - quantitative separation of Zr4+ and W6+ from other metal ions and alloy components. J.
Planar Chromatogr. 13, 93-100 (2000). Study of the retention of tungsten and zirconium on plain and TBA impregnated
silica gel layers with butanol - 8 M HNO3 and aqueous HNO3 mobile phases, as of the effect of pH on hRf values.
Butanol was found most effective in mixed mobile phases. Simple, inexpensive and convenient technique.
33a
35. Other technical products and complex mixtures
85 144 M.J. FABIANSKA, (Silesian Univ., Fac. of Earth Sci., 42-700 Sosnowiec, Bedzinska Street 60, Poland): Preliminary
TLC study of organic oxygen and nitrogen compounds in extracts of organic matter from fossilized wood. J.
Planar Chromatogr. 13, 20-24 (2000). TLC of phenols, aldehydes, ketones, quinones, carboxylic acids, aniline, pyridine,
quinoline, acridine, pyrrole, indole, carbazole derivatives on silica gel with dichloromethane. Detection by UV, standard
visualization agents and 0.5% 2,2’-bipyridine and 0.5% FeCl3 in ethanol, 0.3% ninhydrin in 1-propanol with acetic acid,
1% picryl chloride in ethanol and 4-dimethylaminobenzaldehyde in 6 M HCl and ethanol.
35d
85 145 N.-G. HADARUGA, E. ALEXA, C. JIANU, I. JIANU, (Banat’s Univ. of Agricult. Sciences, Fac. of Agroffood Products
Techn., Calea Aradului 119, 1900 Timisoara, Romania): TLC of tensioactive reagents used as cleansers in the food
industry. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst.
for Med. Plants, p. 205-217. TLC of cetyldimethylbenzil ammonium chloride, cetyltrimethyl ammonium bromide,
tributylmethyl ammonium hydride, laurylpyridium chloride, distearyldemethyl ammonium, benziltrimethyl ammonium
hydride, ATO-368, AT-196, n-dodecilamine, AT22-300 on aluminum oxide with chloroform - NH3 - methanol 8:1:1 and
on silica gel G with ethyl acetate - methanol - NH3 13:3:4 up to 15 cm. TLC of other quaternary ammonium salts and
tertiary amines on silica gel G with chloroform - pyridine - methanol 4:3:3 for 150 min (this was the best system for
separation of quaternary ammonium salts) or 5:3:2.
35c
85 146 G. KATAY*, L. LITINOVA, E. MINCSOVICS, E. MELENEWSKAYA, E. TYHIHAK, (*Plant Protection Inst., Hungarian
Acad. of Sci., Budapest, P.O. Box 102, H-1525 Hungary): Comparative study of the separation of styrene
oligomers by TLC and OPLC. J. Planar Chromatogr. 12, 340-344 (2000). Improved TLC method TLC of styrene
oligomers with peak molecular weights (Mp) 400, 700, and 900 Da (incl. their trimers, tetramers, pentamers etc.up to the
dodecamers) on silica gel with n-heptane - toluene 3:1. Also OPLC on TLC and HPTLC layers. Detection by measuring
the optical density at 260 nm.
35c
85 147 A. MATUSZEWSKA, L. KARWOWSKI, (Fac. of Earth Sci., Silesian Univ., 41-200 Sosnowiec, 60 Boedzieska Str.,
Poland): Thin-layer chromatography in investigation of the chemical structure of Baltic amber. J. Planar
Chromatogr. 13, 140-145 (2000). Preparative TLC for separation of the compounds present in the ethanol extract into
aliphatic, aromatic, and polar fractions, on silica gel with n-hexane - dichloromethane 4:1. Analytical TLC was used for
the identification of organic esters, a-keto acids, a-hydroxy acids, aldehydes, ketones, aliphatic, aromatic and
unsaturated hydrocarbons, terpenoids and steroids on silica gel, with different mobile phases, the one used most often
was n-hexane - benzene - methanol 2:6:1. Compounds were identified on the basis of the color of the spots formed by
use of different visualization reagents, and by comparison with those of available reference compounds.

35d
36. Thin-layer electrophoresis
85 148 A. GUTTMAN, (Novartis Agricultural Discovery Institute, La Jolla, CA 92121, USA): Electric field mediated
separation of biopolymers on planar glass microchips. Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography
2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 47-56. High throughput analysis of
biopolymers via a microchip (a re-useable multilane, ultra-thin-layer gel electrophoresis approach). This technology
combines the advantages of conventional planar slab gel electrophoresis and capillary electrophoresis. Visualization by
"in migratio" complexation during the electrophoresis process with ultra sensitive fluorescent agents. Detection by
fiber-optic bundle based integrated laser induced fluorescence scanning - avalanche photodiode imaging system.
36
37. Environmental analysis
85 149 H. KUTSCH*, U. SCHOEN, (*Catholic Univ. Eichstätt, Phys. Geogr., Landscape Ecology, Geolab., Ostenstrasse 18,
D-85072 Eichstätt, Germany): A simplified HPTLC screening method for the estimation of the PAH content in soil
samples. Fresenius J. Anal. Chem. 367, 279-283 (2000). HPTLC of a EPA-PAH16 environmental standard and B[a]P,
DB[ah]A, B[ghi]P, In[1,2,3-cd]P, Ant/Flu, B[b]F, B[k]F on silica gel RP-18 with ethanol - dichloromethane 1:1, mixed
immediately before the run. Visualization under UV 366 nm. - Achievement of a deliberately incomplete separation of
the visual fluorescing fraction of PAH and the total EPA-PAH16 content into a few fingerprint-like compressed bands
within a determined "PAH window".
37d
85 150 D.R. NEMERGUT, R.M. JOHNSON, K.G. WUNCH*, J.W. BENETT, (Dept. of Cell and Molecular Biol., Tulane Univ.,
2000 Percival Sterne Hall, New Orleans, LA 70118, USA): Extraction and quantification of benzo[a]pyrene by
reversed phase thin layer chromatography. J. Liqu. Chromatogr. 23, 579-586 (2000). HPTLC of benzo[a]pyrene on
hydrocarbon impregnated RP-18 plates with methanol - acetonitrile 1:1. Quantification by densitometry at 370 nm.
37d, 5b
38. Chiral separations
85 151 R. BHUSHAN*, G. THUKU THIONG'O, (*Dept. of Chem., Univ. of Rookee, Roorkee 247 667, India): Separation of the
enantiomers of dansyl-DL-amino acids by normal-phase TLC on plates impregnated with a macrocyclic
antibiotic. J. Planar Chromatogr. 13, 33-36 (2000). Direct racemic resolution of dansyl-DL-amino acids on silica gel
plates impregnated with vancomycin (0.34 mM) as chiral selector. The mobile phase enabling successful resolution of
most of the racemic dansyl amino acids were acetonitrile - 0.5 M aqu. NaCl 5:2 and 14:3. Detection with a fixed
dual-wavelength UV light at 254 nm. Detection limit 2.1µg. New sorbent for separation of chiral compounds resulting in
a simple, rapid, sensitive, cost-effective method.
38, 18a


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CBS Edition 85

The CBS classification system

© 2015 CAMAG. All rights reserved.

2. Fundamentals, theory and general

© 2015 CAMAG. All rights reserved.

85 012 I. MALINOWSKA, (Dept. of Adsorption and Planar Chromatography, M. Curie-Sklodowska Univ., Pl M.

85 013 A. PELANDER, J. SUMMANEN, T. YRJOENEN, H. HAARIO, I. OJANPERÄ, H. VUORELA*, (*Div. of Pharmacogn.,

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

5. Hydrocarbons and halogen derivatives

© 2015 CAMAG. All rights reserved.

8. Substances containing heterocyclic oxygen

85 037 A. DEGENHARDT, H. KNAPP, P. WINTERHALTER*, (*Inst. für Lebensmittelchemie, Technische Universität

© 2015 CAMAG. All rights reserved.

11. Organic acids and lipids

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

14. Steroid glycosides, saponins and other terpenoid glycosides

16. Nitro and nitroso compounds

© 2015 CAMAG. All rights reserved.

17. Amines, amides and related nitrogen compounds

18. Amino acids and peptides, chemical structure of proteins

21. Purines, pyrimidines, nucleic acids and their constituents

© 2015 CAMAG. All rights reserved.

85 073 W. ZWICKENPFLUG, (Walther Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität,

23. Other substances containing heterocyclic nitrogen

27. Vitamins and various growth regulators

28. Antibiotics, Mycotoxins

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29. Pesticides and other agrochemicals

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30. Synthetic and natural dyes

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31. Plastics and their intermediates

32. Pharmaceutical and biomedical applications

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

© 2015 CAMAG. All rights reserved.

33. Inorganic substances

© 2015 CAMAG. All rights reserved.

35. Other technical products and complex mixtures

© 2015 CAMAG. All rights reserved.

36. Thin-layer electrophoresis

37. Environmental analysis

38. Chiral separations

© 2015 CAMAG. All rights reserved.

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85 013 A. PELANDER, J. SUMMANEN, T. YRJOENEN, H. HAARIO, I. OJANPERÄ, H. VUORELA, (Div. of Pharmacogn.,

85 037 A. DEGENHARDT, H. KNAPP, P. WINTERHALTER, (Inst. für Lebensmittelchemie, Technische Universität