21

1. A primosome can be defined as

A. t he enzyme that synthesizes primers fro M13 phage replication.

B. a

collection of proteins needed for synthesis of primers for replicating DNA.
C. a collection of RNA primers.

D. a DNA molecule that is primed and ready for replication.

E. a n area of DNA near the replication fork waiting to be replicated.

2. Which of the following is the primase that synthesizes primers during DNA replication in E. coli?

A. D

naA
B. R

NA polymerase
C. HU protein

D. DnaB

E. D
naG

3. Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the
"sliding clamp"?

A. α


B. β


C. γ

D. χ

E. θ

4. Which of the following enzymes is responsible for decatenation of Salmonella typhimurium DNA
during replication?

A. t opoisomerase I

B. t opoisomerase II

C. topoisomerase III

D. topoisomerase IV

E. t opoisomerase V


5. Which of the following DNA polymerase III subunits is needed to help "clamp" the β-clamp onto
DNA?

A. α


B. ε

C. γ complex

D. τ

E. θ

6. Given the following cell types, which do you propose would have the highest levels of telomerase
activity?

A. m

onkey liver cell
B. m

acrophage
C. Schwann cell

D. colon cancer cell

E. o
steoclast

7. In vivo, the DNA polymerase III holoenzyme can synthesize DNA at a rate of

A. 2

50 nt/sec.
B. 5

00 nt/sec.
C. 750 nt/sec.

D. 1000nt/sec.

E. 2
000nt/sec.

8. Which of the following is not part of the E. coli primosome?

A. D

naA
B. D

naB
C. oriC

D. TerE

E. D
naG


9. Put the following steps of E. coli primosome assembly in the correct order.
(1) Primase binds.
(2) DnaA binds to oriC at dnaA boxes.
(3) DnaB binds to the open complex.
(4) DnaA, RNA polymerase, and HU protein melt the DNA.

A. 2

, 4, 1, 3
B. 2

, 4, 3, 1
C. 4, 1, 2, 3

D. 4, 2, 1, 3

E. 3
, 4, 1, 2

10. Yeast origins of replication are located within ____________ that are composed of four important
regions.

________________________________________

11. DNA polymerase III is a highly ___________ enzyme, meaning once it binds DNA it catalyzes the
addition of several hundreds of nucleotides without falling of the DNA.

________________________________________

12. The DNA polymerase III core plus the ____________ is needed to replicate DNA processively.

________________________________________

13. The DNA polymerase III holoenzyme contains two core enzymes, one that is responsible for
___________ strand synthesis and the other responsible for ____________strand synthesis.

________________________________________

14. The ends of eukaryotic chromosomes are composed of short GC-rich repeat sequences called
____________.

________________________________________

15. Mammalian cells can protect their single stranded telomeric DNA by forming a ______ loop.

________________________________________

16. Region ______ is an 11-bp highly conserved consensus sequence found in the autonomously
replicating sequences of yeast.

________________________________________

17. Loading of the β-clamp onto DNA by the clamp loader is an _________-dependent process.

________________________________________

18. In E. coli, DNA replication begins at a unique site, oriC, in the genome.

True False

19. The chromosomal origins of replication vary greatly among bacteria, so much so, that it is hard to
propose an origin of replication consensus sequence.

True False

20. Replication of the SV40 genome is dependent on the viral large T antigen, which has primase
activity.

True False

21. DNA replication in eukaryotes always begins at one origin of replication on each chromosome.

True False

22. After DNA replication, bacterial genomes must undergo decatenation before the cell can divide.

True False

23. Bacterial chromosomes are the only chromosomes known to require decatenation during
replication.

True False

24. During lagging strand synthesis, it is not uncommon for the DNA polymerase III holoenzyme to
dissociate completely from the DNA template for approximately two seconds.

True False

25. Without telomeres, linear eukaryotic chromosomes would get shorter and shorter with each round
of DNA replication.

True False

26. Telomerase enzyme activity was first observed in extracts from Tetrahymena micronuclei.

True False

27. Telomerase is a unique enzyme in that it is composed of only RNA.

True False

28. All eukaryotes must protect their telomeres from nucleases and double strand break repair
enzymes.

True False

29. The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA
polymerase III β-subunit.

True False

30. The DNA polymerase III holoenzyme γ-complex can act as a clamp loader and unloader.

True False

 21 Key

1. A primosome can be defined as

A. the enzyme that synthesizes primers fro M13 phage replication.

B. a collection of proteins needed for synthesis of primers for replicating DNA.

C. a collection of RNA primers.

D. a DNA molecule that is primed and ready for replication.

E. an area of DNA near the replication fork waiting to be replicated.

Blooms Level: 1. Remember

Section: 21.01
Weaver - Chapter 21 #1


2. Which of the following is the primase that synthesizes primers during DNA replication in E.
coli?

A. DnaA

B. RNA polymerase

C. HU protein

D. DnaB

E. DnaG

Blooms Level: 1. Remember

Section: 21.01
Weaver - Chapter 21 #2


3. Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the
"sliding clamp"?

A. α

B. β

C. γ

D. χ

E. θ

Blooms Level: 1. Remember

Section: 21.01
Weaver - Chapter 21 #3



4. Which of the following enzymes is responsible for decatenation of Salmonella typhimurium

DNA during replication?

A. topoisomerase I

B. topoisomerase II

C. topoisomerase III

D. topoisomerase IV

E. topoisomerase V

Blooms Level: 1. Remember

Section: 21.03
Weaver - Chapter 21 #4


5. Which of the following DNA polymerase III subunits is needed to help "clamp" the β-clamp
onto DNA?

A. α

B. ε

C. γ complex

D. τ

E. θ

Blooms Level: 1. Remember

Section: 21.01
Weaver - Chapter 21 #5


6. Given the following cell types, which do you propose would have the highest levels of
telomerase activity?

A. monkey liver cell

B. macrophage

C. Schwann cell

D. colon cancer cell

E. osteoclast

Blooms Level: 3. Apply

Section: 21.02
Weaver - Chapter 21 #6



7. In vivo, the DNA polymerase III holoenzyme can synthesize DNA at a rate of

A. 250 nt/sec.

B. 500 nt/sec.

C. 750 nt/sec.

D. 1000nt/sec.

E. 2000nt/sec.

Blooms Level: 2. Understand

Section: 21.02
Weaver - Chapter 21 #7


8. Which of the following is not part of the E. coli primosome?

A. DnaA

B. DnaB

C. oriC

D. TerE

E. DnaG

Blooms Level: 1. Remember

Section: 21.01
Weaver - Chapter 21 #8


9. Put the following steps of E. coli primosome assembly in the correct order.
(1) Primase binds.
(2) DnaA binds to oriC at dnaA boxes.
(3) DnaB binds to the open complex.
(4) DnaA, RNA polymerase, and HU protein melt the DNA.

A. 2, 4, 1, 3

B. 2, 4, 3, 1

C. 4, 1, 2, 3

D. 4, 2, 1, 3

E. 3, 4, 1, 2

Blooms Level: 5. Evaluate

Section: 21.01
Weaver - Chapter 21 #9


10. Yeast origins of replication are located within ____________ that are composed of four
important regions.

autonomously replicating sequences (ARSs)

Blooms Level: 1. Remember
 Section: 21.01
Weaver - Chapter 21 #10


11. DNA polymerase III is a highly ___________ enzyme, meaning once it binds DNA it catalyzes
the addition of several hundreds of nucleotides without falling of the DNA.

processive

Blooms Level: 2. Understand
Section: 21.02
Weaver - Chapter 21 #11


12. The DNA polymerase III core plus the ____________ is needed to replicate DNA processively.

β-clamp

Blooms Level: 2. Understand
Section: 21.02
Weaver - Chapter 21 #12


13. The DNA polymerase III holoenzyme contains two core enzymes, one that is responsible for
___________ strand synthesis and the other responsible for ____________strand synthesis.

leading, lagging

Blooms Level: 2. Understand
Section: 21.02
Weaver - Chapter 21 #13


14. The ends of eukaryotic chromosomes are composed of short GC-rich repeat sequences called
____________.

telomeres

Blooms Level: 1. Remember
Section: 21.03
Weaver - Chapter 21 #14


15. Mammalian cells can protect their single stranded telomeric DNA by forming a ______ loop.

t

Blooms Level: 1. Remember
Section: 21.03
Weaver - Chapter 21 #15


16. Region ______ is an 11-bp highly conserved consensus sequence found in the autonomously
replicating sequences of yeast.

A

Blooms Level: 1. Remember
Section: 21.01
Weaver - Chapter 21 #16


17. Loading of the β-clamp onto DNA by the clamp loader is an _________-dependent process.

ATP

Blooms Level: 2. Understand
Section: 21.01
Weaver - Chapter 21 #17


18. In E. coli, DNA replication begins at a unique site, oriC, in the genome.

TRUE

Blooms Level: 1. Remember
Section: 21.01
Weaver - Chapter 21 #18


19. The chromosomal origins of replication vary greatly among bacteria, so much so, that it is hard
to propose an origin of replication consensus sequence.

FALSE

Blooms Level: 2. Understand
Section: 21.01
Weaver - Chapter 21 #19


20. Replication of the SV40 genome is dependent on the viral large T antigen, which has primase
activity.

FALSE

Blooms Level: 2. Understand
Section: 21.01
Weaver - Chapter 21 #20


21. DNA replication in eukaryotes always begins at one origin of replication on each
chromosome.

FALSE

Blooms Level: 2. Understand
Section: 21.01
Weaver - Chapter 21 #21


22. After DNA replication, bacterial genomes must undergo decatenation before the cell can
divide.

TRUE

Blooms Level: 2. Understand
Section: 21.03
Weaver - Chapter 21 #22



23. Bacterial chromosomes are the only chromosomes known to require decatenation during
replication.

FALSE

Blooms Level: 2. Understand
Section: 21.03
Weaver - Chapter 21 #23


24. During lagging strand synthesis, it is not uncommon for the DNA polymerase III holoenzyme to
dissociate completely from the DNA template for approximately two seconds.

FALSE

Blooms Level: 2. Understand
Section: 21.02
Weaver - Chapter 21 #24


25. Without telomeres, linear eukaryotic chromosomes would get shorter and shorter with each
round of DNA replication.

TRUE

Blooms Level: 2. Understand
Section: 21.03
Weaver - Chapter 21 #25


26. Telomerase enzyme activity was first observed in extracts from Tetrahymena micronuclei.

FALSE

Blooms Level: 1. Remember
Section: 21.03
Weaver - Chapter 21 #26


27. Telomerase is a unique enzyme in that it is composed of only RNA.

FALSE

Blooms Level: 2. Understand
Section: 21.03
Weaver - Chapter 21 #27


28. All eukaryotes must protect their telomeres from nucleases and double strand break repair
enzymes.

TRUE

Blooms Level: 2. Understand
Section: 21.03
Weaver - Chapter 21 #28



29. The eukaryotic processivity factor PCNA serves the same function as the prokaryotic DNA
polymerase III β-subunit.

TRUE

Blooms Level: 3. Apply
Section: 21.02
Weaver - Chapter 21 #29


30. The DNA polymerase III holoenzyme γ-complex can act as a clamp loader and unloader.

TRUE

Blooms Level: 2. Understand
Section: 21.02
Weaver - Chapter 21 #30

 21 Summary

Category # of Questions
Blooms Level: 1. Remember 12
Blooms Level: 2. Understand 15
Blooms Level: 3. Apply 2
Blooms Level: 5. Evaluate 1
Section: 21.01 13
Section: 21.02 8
Section: 21.03 9
Weaver - Chapter 21 30