Service Manual Ce4200 Series Uv-Visible Detector
Service Manual Ce4200 Series Uv-Visible Detector
CE4200 SERIES
UV-VISIBLE DETECTOR
EDITION NO : 1
RELEASE NO : 5
DATE OF RELEASE : July 2009
FOR YOUR OWN SAFETY, DO NOT PERFORM ANY SERVICING UNLESS YOU
ARE QUALIFIED TO DO SO.
WARNING:
SOME MODELS HAVE A LITHIUM BATTERY BT1 MOUNTED ON THE MPU PCB,
NEAR EPROM U3. CARE MUST BE TAKEN TO ENSURE THAT EXPOSED
METAL PARTS OF THE I.C. EXTRACTION TOOL DO NOT MAKE ELECTRICAL
CONTACT WITH THE BATTERY.
Contents
1 INSTALLATION
1.1 LOCATION
1.2 STACKING
1.3 COLUMN AND INJECTION PANEL
1.4 CONNECTION TO MAINS POWER
2 MAINTENANCE
2.1 OPENING THE INSTRUMENT
2.2 CHANGING THE DEUTERIUM LAMP
2.3 ALIGNING THE DEUTERIUM LAMP
2.4 ABSORBANCE CALIBRATION
2.5 WAVELENGTH CALIBRATION
2.6 WAVELENGTH ADJUSTMENT
2.7 CLEANING THE FLOW CELL
2.8 RESOLUTION VALIDATION
2.9 STRAY LIGHT MEASUREMENT
3 FAULT FINDING
3.1 POWER FAILURE
3.2 ERROR MESSAGES
3.3 FAILURE OF START UP TEST AND CALIBRATION ROUTINE
3.4 NOT POSSIBLE TO SET ABSORBANCE ZERO
3.5 NOT POSSIBLE TO SET ZERO TRANSMISSION
3.6 UNSTABLE BASELINE, ABSORBANCE ZERO DRIFT
3.7 SUDDEN CHANGES IN BASELINE
3.8 WAVELENGTH NOT REPRODUCING
3.9 DEUTERIUM LAMP DOES NOT STRIKE
3.10 CHECKING THE DEUTERIUM LAMP CURRENT
3.11 REPLACING THE POWER TRANSISTOR
3.12 MAINS AND POWER SUPPLY FUSES
3.13 POWER SUPPLY TEST POINTS
3.14 REPLACING THE MPU AND DETECTOR INTERFACE PCB’S
3.15 REPLACING THE KEYBOARD
3.16 REPLACING THE POWER SUPPLY PCB
3.17 REPLACING THE PHOTO DIODE PCB
4 EXTERNAL CONNECTIONS
4.1 PARALLEL PRINTER
4.2 CONTROL I/O
4.3 SIGNAL OUT
4.4 SERIAL RS-232
5 PART NUMBERS
5.1 CELLS
5.2 SPARES AND CONSUMABLES
5.3 ELECTRICAL COMPONENTS
5.4 MECHANICAL COMPONENTS
6 FIGURES
FIGURE 1 THE FRONT PANEL
FIGURE 2 THE REAR PANEL
FIGURE 3 DEUTERIUM LAMP MOUNTING
FIGURE 4 LAYOUT OF 4200/4300 23 00 PHOTODIODE PCB
FIGURE 5 LAYOUT OF 4200 55 00 CONTROL PANEL PCB
FIGURE 6 LAYOUT OF 4200 75 00 DETECTOR INTERFACE PCB
FIGURE 7 LAYOUT OF 1201 80 00 HPLC MPU PCB
8 HARDWARE CHANGES
9 TECHNICAL SPECIFICATION
9.1 SCANNING CE4300
9.2 DUAL WAVELENGTH CE4200
9.3 UV/VISIBLE DETECTOR CE4201
1.2 Stacking
The Detector is designed as part of the Adept series Modular HPLC System, as such
the individual components may be stacked with other units of the system. Note the
Detector(s) should always be on the top of the stack.
b Gently raise the lid from the rear of the instrument and slide it out backwards.
Note: To replace the lid, ensure that the front edge locates under the front lip of the
instrument prior to replacing the two screws.
c Remove the two counter sunk screws and washers from either end of the side
panels and remove the side panels.
Caution!
The lamp and lamp housing become very hot while the instrument is switched
on. Wait for these items to cool before attempting this maintenance procedure.
Do not touch the lamp while it is hot. Wear gloves when handling the lamp to
prevent oily residues being left on the envelope.
Caution!
The deuterium lamp emits harmful ultraviolet radiation. It is imperative that you
switch the instrument off before attempting this maintenance procedure.
a Switch the instrument off and wait for 30 minutes for the deuterium lamp and
housing to cool down.
b Loosen the lamp housing retaining screw (Figure 2 H) and lift off the housing.
c Carefully undo the spring anchor screw (A) to release the lamp retaining
spring (B).
e Take the new deuterium lamp and feed the wires downwards and connect them
to the connector block as follows:
f The wires should be looped under and behind the lower end of the lamp.
g Lower the lamp so that its side arm rests in the V of the holder. Tension the
lamp retaining spring (B) over the side arm of the lamp using the spring
anchor screw (A) threaded through the end loop of the spring, and replace the
screw. The lamp should now be held in position.
Caution!
Be aware that the lamp and lamp housing become very hot while the instrument
is switched on.
Caution!
The deuterium lamp emits harmful ultraviolet radiation. It is imperative that you
wear special anti-UV goggles to avoid damaging your eyes.
Caution!
The HPLC detector has no internal parts that are serviceable by an operator. The
only reason for removing the casing is to align the deuterium lamp. The lid and
right side panel are removed during this procedure. Do not remove any other
parts of the casing. Do not touch any internal parts of the instrument.
b Remove the top cover of the instrument. Note the position and order of all
components, for later re-assembly.
d Use the techniques described for the fine adjustment (next section) to position
the lamp so that the beam falls symmetrically onto the centre of the slot (A).
e Replace and secure the lamp housing. Replace the instrument side panel.
Note: You can check noise and drift performance by using the dummy cell.
b Fit the dummy cell into the holder in the cell compartment.
c Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Calibration’
and press E to select. The instrument will calibrate.
d Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Beam Mode’
and press E to select. Use the arrow keys to select 1, sample beam, and press
E.
e Remove the lamp housing by slackening the retaining screw (Figure 2, H) and
lifting off the housing.
i Use screw (5) to adjust the horizontal position of the lamp for maximum
transmission (highest % reading) as shown by the front panel display.
j Adjust the position of the lamp until the maximum energy is achieved. Tighten
the locking screws after the adjustment.
k Press the MODE key and select ‘Service Mode’. Use the arrow keys to select
‘Beam Mode’. Select 2, double beam
The instrument will perform a zero and switch back to absorbance readings.
c Select the type of filter, the number of filters, and the tolerance.
e With the special cell holder fitted in the beam press the ‘Zero’ button.
f Insert each filter in turn and note the value. The results are sent to the printer
connected to the printer port in the rear panel of the detector.
Note: Insert the filters with the identifiers on the upper end and with the open side of
filter holders facing the rear of the instrument.
If validation is unavailable manually read the absorbance of the filters and
make a note.
Check the noted values against the calibration certificate taking into account the
certified uncertainty and the instrument tolerance for each filter.
i.e. Value ± uncertainty ± 0.005A or ±1%.
2.5.1 Note: The precise wavelength value(s) will depend upon the individual filter and its
calibration certificate.
c Select the type of filter you are using, the tolerance and the number of
wavelengths.
f The results are sent to the printer connected to the printer port in the rear panel
of the detector.
g If the obtained results are outside specification then refer to section, 2.6
wavelength adjustment, to adjust the wavelength offset.
2.5.2 Note: The precise wavelength value(s) will depend upon the individual filter and its
calibration certificate.
a Using the wavelength button drive the instrument to one of the above
wavelengths.
b Using the mode button select PEEK SEEK and E to enter, and follow the
prompts to run the reference.
c When prompted, insert the filter and press E. Note the reported value on the
screen and repeat the above for the wavelengths to be checked.
b Under service mode enter ‘WL Offset’, the display will indicate the current
stored offset value in nm
c Enter a new offset value to bring the measured peaks within specification.
a Refer to figure 11. Loosen the two compression screws F which connect the
inlet and outlet tubes.
c Slide out the internal parts of the flow cell noting the order for re-assembly.
d Inspect the bore of the flow cell insert I to be clear of all forms of fibres or
partial obstructions and clean or replace as required.
e The windows should be cleaned with a solvent such as acetone or alcohol and
dried using a soft cloth or tissue.
g Make sure that the p.t.f.e. washer D is fitted between the Kel-F insert and the
window at the outlet end of the cell.
h The end plugs should be tightened evenly ensuring that the insert remains
central with the flats correctly aligned i.e., normal to the inlet and outlet tubes.
These tubes may then be inserted and tightened using the fingers only.
b Press the MODE key to run through the options in the menu until the
‘Validation’ option is displayed. Press the E key to select the option.
c Press the ARROW keys until the ‘Res Validation’ option is displayed. Press
the E key to select the option.
d The results are sent to the printer connected to the printer port in the rear panel
of the detector.
a Hold down the OFFSET key during power up. Release the OFFSET key when
the message ‘Service Calibration’ appears. The instrument powers up in
service mode.
c Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Calibration’
and press E to select. The instrument will calibrate.
d Fill a clean cell with distilled or de-ionised water then place the cell into the
beam, set the wavelength to 220nm.
e Press the MODE key several times, until ‘Service Mode’ is displayed. Press
the E key to select service mode. Use the arrow keys to display ‘Beam Mode’
and press E to select. Use the arrow keys to select 1, sample beam, and press
E.
f Drain the cell and then rinse out and fill with the aqueous sodium iodide
solution. Insert a cell containing the sodium iodide solution into the beam, the
reading should be less than 0.02%.
g Press the MODE key and select ‘Service Mode’. Use the arrow keys to select
‘Beam Mode’. Select 2, double beam.
The instrument will perform a zero and switch back to absorbance readings.
b Check the mains power fuse on the rear panel of the instrument and replace, if
failed, with the correct value fuse. Try to establish the cause of fuse failure.
b Too much light reaching the detector. Error message E33 displayed.
This may be the result of the flow cell compartment lid not fitted correctly or
the flow or dummy cell not being fitted into the cell compartment.
b Air bubbles in flow cell, These can often be dislodged by pinching the p.t.f.e.
outlet tube.
a Air bubbles in the cell. Use only well degassed and filtered solvent. Check for
leaks or a dirty cell. See section 2.7
b Leaking cell or connection causing solvent vapour to enter the light path
causing absorption of energy from the light path.
c Well retained compounds which are eluted from the column after a
considerable delay. Turn off solvent flow, a steady baseline should result If
this is the cause purge the column at a high flow rate or use a stronger solvent.
d If the heat exchanger is not fitted between the column and the cell, temperature
instability could be the cause. Check for drafts etc.
b Unstable deuterium lamp. This often gives rise to fairly regular steps in the
baseline. The effect will still be present when the solvent flow is turned off or
the dummy cell is fitted.
Sometimes the lamp can be corrected by switching off and on a few times in
succession.
b Cam arm return spring not functioning properly. Check visually and also
gently lift arm off cam to test spring tension.
c Check the belt tension is not too loose, check pinion gear is locked tight to
drive shaft.
a Check that the deuterium lamp is in fact off. Remove the cover of the lamp
house. If the lamp glows with a bright blue light (DANGER to eyes from UV
radiation) it has struck and the indicator circuit has failed.
If a red glow is visible inside the lamp structure, this shows that the lamp
heater is on.
Proceed to section b below, if no light can be seen at all proceed to section c.
b The lamp should operate for 1 year from the date of supply. If the lamp is
older, then either replace the lamp, see section 2.2, or check the fuse for the
deuterium supply mounted on the rear panel.
If it has blown there is a fault on the PCB, a short circuit in the deuterium lamp
or a faulty power transistor. Locate the fault by inspection or replacement.
c If no glow can be seen from the deuterium lamp, the heater is open circuit or
there is a fault in the power supply. Either the lamp must be replaced, see
section 2.2, or it may be possible to cure the fault by changing the deuterium
lamp power supply board.
Switch off and disconnect the instrument from the mains supply.
a Remove the four screws that hold the transformer housing to the rear panel
b Slacken the two M4 cap head allen screws (that secure the heat sink to the
transformer housing) approximately two turns then slide the heat sink down
off its fixing.
c Remove the two screws that secure the transistor and then unsolder the two
remaining wires, noting the connections and the 2K2 resistor in the base lead.
d Ensure that the new transistor is insulated from the heat sink by top hat
insulators and a silicone rubber insulator or equivalent. Check the insulation
with a meter. Re-solder the wire connections as noted.
a TP2, +5 volts
e There is also a +16 volt DC unregulated supply, this can be measured on Pin 1
of connector PL1, again relative to TP1, 0 volts.
f The Deuterium lamp running voltage (HT supply) can also be checked by
measuring the voltage on wire 46, either on Pin 3 of connector PL4 or on the
fuse holder for Fuse FS2, again relative to TP1, 0 volts. This voltage should
be ~120V D.C. (max.140V, min 100V).
Warning this voltage can be lethal. Refer to Figures 8 & 10.
Note: only the voltage measured on TP5 is adjustable, see section 3.10 above.
Switch off and disconnect the instrument from the mains supply.
b Remove the ribbon cables (PL 1 and PL8) from the Keyboard and the ADC
c Remove the screw terminal plugs (PL 10 and PL11) from the Power Supply
d Remove the 6 pin connectors (PL6 &7) from the Wavelength and Filter
motors
f Remove the two hex nuts that secure 9 way D plug (PL9) to the rear panel
g Remove the 4 M3 nuts and washers that hold the interface and MPU PCB’s to
the left side of the monitor chassis and lift them out carefully.
Note: the MPU plugs into the interface PCB and can be separated once the interface
is clear of the instrument
Switch off and disconnect the instrument from the mains supply
b Remove the 4 screws with shake proof washers from each corner of the front
panel and ease the panel forward.
c Detach the keyboard from the front panel by removing the 4 screws on the rear
face of the front panel that attach the control panel to the front panel, noting
the earth bond wire under one of the screws. Release the ribbon cable that
links it to the interface PCB (PL1).
e Reset the screen contrast by use of the up/down keys after the instrument has
been switched on.
Switch off and disconnect the instrument from the mains supply
b Remove the 4 screws and shake proof washers that secure the rear panel to the
rear of the instrument and ease the panel way from the rear chassis.
c Remove the four power supply plugs (PL1, PL2, PL3 & PL4) from the PCB.
d Release the two spring clips that secure the PCB to the inner rear panel then
ease the PCB out of the back of the monitor.
Switch off and disconnect the instrument from the mains supply
b Remove the 4 screws with shake proof washers from each corner of the front
panel and ease the panel forward.
d Remove the four M3 nuts and washers that secure corners of the PCB
e Ease the PCB forward slightly and then unsolder the two wires connected to
terminals P3 & P4, sleeved Red & Blue, the connections from the reference
photo diode, noting the orientation. Refer to Figure 4
f Continue to ease the PCB forward, carefully extracting the sample photo diode
from it’s mounting. Note, the sample photo diode is soldered to terminals P1
& P2 on the rear of the PCB
Keys
and Use these keys to scroll through menu options and to increase or decrease a value
( ARROW keys) shown on the electronic display. They are also used to adjust the screen contrast.
E (EVENT CANCEL) This key provides three functions. It is used to accept any entered or default value. It
is also the event marker. When you press the key at any time, other than to accept or
cancel a value, it causes a pen deflection of an attached chart recorder. Holding the
key pressed clears any entered value to 0.
MODE Press this key to display the ‘mode’ menu options, which are mainly concerned with
configuring the detector for use, and setting up pump gradients.
METHOD Press this key to display the ‘method’ menu options, where you can save current
settings and parameters into methods.
SCAN If you have purchased the scanning option (available for the CE 4200 only), use this
key to display the ‘scan’ menu options. They allow you to scan a range of
wavelengths to find the one that is most appropriate for the sample that you are
measuring.
ZERO Zeros the instrument electronic display and signal output.
OFFSET Allows you to change the offset that has been set for the signal out port.
W’LENGTH Allows you to set the wavelength.
RANGE Allows you to change the range that has been set for the signal out port.
A B C E F G
H I J K L M
PL5 4200-92-00
PARALLEL PRINTER PARALLEL PRINTER
PL1 4200-91-00 PL8
MONITOR I/F ADC
PL3 PL3-1 0V
4200/4201/4300 Service
MONITOR CONTROL PANEL DIGITAL I/O
PL3-2 IN1
PL3-3 IN2
PL3-4 IN3
PL3-5 0V
PL1 4200-90-00 PL1 PL3-6 OUT1
MONITOR I/F CONTROL PANEL
PL3-7 OUT2
PL3-8 OUT3
HPLC MPU
4200-94-00
PL3-1 20 PL11-1 PL2 SK1
+12V +12V DIN41612 CONNECTOR DIN41612 CONNECTOR
PL3-3 21 PL11-3
0V 0V
NOTE:
PL3-4 22 PL11-4 THE HPLC MPU IS MOUNTED DIRECTLY
-12V -12V
S6 : 11P
ONTO THE HPLC MONITOR INTERFACE
PL3-5 23 PL11-5
0V 0V
VIA THE DIN41612 CONNECTORS.
PL3-6 24 PL11-6
D2ENABLE D2ENABLE
PL3-7 25 PL11-7
D2STRIKE D2STRIKE 1201-80-00
PL3-8 26 PL11-8
D2ON D2ON
PL3-10 28 PL11-10
BRESET BRESET
M1
4200-93-00 WAVELENGTH MOTOR
PL1-1 30 PL10-1
VMOTOR VMOTOR
PL7 1 P+
FILTER MOTOR[1..4]
PL1-2 31 PL10-2
0V 0V
2 P-
10 PL1-3 32 PL10-3
L VCC VCC
M2
60 PL1-4 33 PL10-4 FILTER MOTOR 5 Q+
N VCC VCC
PL1-5 34 PL10-5 1 P+ 6 Q-
0V 0V
PL1-6 35 PL10-6 3 P-
0V 0V
5 Q+
W100I001.SCH
6 Q-
PL1 54 1 4200-75-00
A 1 A
3 4 FRONT PANEL
2
50 2 5 53 1
3
1 6
MAINS 51 LEFT SIDE FRAME CECIL INSTRUMENTS LIMITED
INPUT 52 1
0
FILTER Title
RIGHT SIDE FRAME CE 4200 MONITOR WIRING DIAGRAM
BASE PLATE BACK PANEL
Size Document Number Rev
B 4200-000 W000I001.SCH 1
Page 9
Figure 9 Detector Wiring Diagram
5 4 3 2 1
1
LIFE INDICATOR
(HOURS)
2
D D
4200/4201/4300 Service
DEUTERIUM LAMP
MONITOR POWER SUPPLY
FS2
PL4-3 46 72
D2 RUN
T1
2
3
TRANSFORMER F0.5A TB1
PL4-10 73 1 2 BLUE
D2 HEATER
BROWN 4 42 RED PL2-1
16V AC1
PL4-1 49 1 2 RED
D2 ANODE
41 RED PL2-2
16V AC2
SW2 PL4-9 76 1 2 BLACK
D2 TRCOL
MAINS 32 YELLOW PL2-3
95V AC1
VOLTAGE 77
15 2A 230V SELECTOR 3 31 YELLOW PL2-4
95V AC2
2 R1
12 2B WHITE 2 22 GREEN PL2-5 PL4-8 75 TR1
15V AC1 D2 TRBASE
C1 MJN11016
1A 21 GREEN PL2-6 2k2
15V AC2
C 220nF 1 BLACK C
250V X2 1B 12 ORANGE PL2-7 PL4-7 74
13V AC1 D2 EMITTER
115V
BLUE 1 11 ORANGE PL2-8
13V AC2
PL3-1 20
+12V +12V
4200-25-01 PL3-3 21
0V 0V
PL3-4 22
-12V -12V
12
61 PL3-5 23
0V 0V
SW1 PL3-6 24
D2ENABLE D2ENABLE
MAINS
S6 : 11P
ON/OFF PL3-7 25
D2STRIKE D2STRIKE
11 PL3-8 26
D2ON D2ON
PL3-9 27
D2HEATON D2HEATON
FS1
T3.15A PL3-10 28
BRESET BRESET\
B B
PL1-1 30
+16V VMOTOR
10
L
PL1-2 31
0V 0V (16)
60
N
PL1-3 32
+5V VCC
PL1-4 33
+5V VCC
PL1-5 34
0V 0V
PL1-6 35
0V 0V
4200-66-00
A A
Title
CE 4200 MONITOR WIRING DIAGRAM
Page 10
Figure 10 Power Supply Wiring Diagram
Figure 11 Flow cell assembly
E
OUTLET
C
H
A B D F C B A
G I
F
INLET E
Scanning Specification
Wavelength Range 190-700nm
Optical Bandwidth 4nm – constant
Digital Resolution 1nm
Wavelength Accuracy 1nm
Absorbance Range 0.001 to 3A
Scan Speeds Up to 1000nm/s – UV scan in 200ms
Baseline Flatness ± 0.002A over most of range
Second Order Filter Fast automatic insertion at 370nm
Noise < ± 0.7 x 10–5 A, 230nm, 2 s T/C, using air test cell
Drift < ±0.7 x 10–4 A/h, 230nm, at equilibrium using air test cell
Overlayed Scans Up to 8 scans may be overlayed
Scan Trigger At start of peak, set absorbance value, point of inflection or
at peak
Number of Peak Scans Up to 100 peaks for a single elution
Scan Storage Up to 100 scans password protected
Multiple Scans of a Peak Up to 8 scans of any peak
Used with DataStream Software Data to PC, PC control, scan triggering, Lambda max. scans,
peak purity and purity calculation, graphical presentation,
overlay, normalisation, disk storage, library store and search,
transfer to Excel etc and many other functions.
General Specification