Biology 9744/01: Nanyang Junior College JC 1 Block Test Higher 2
Biology 9744/01: Nanyang Junior College JC 1 Block Test Higher 2
Biology 9744/01: Nanyang Junior College JC 1 Block Test Higher 2
JC 1 BLOCK TEST
Higher 2
CANDIDATE
NAME
CLASS
BIOLOGY 9744/01
Paper 1 Multiple Choice 26 June 2020
30 minutes
Additional Materials: Multiple Choice Answer Sheet
There are fifteen questions on this paper. Answer all questions. For each question there are four possible
answers A, B, C and D.
Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet.
Each correct answer will score one mark. A mark will not be deducted for a wrong answer.
Any rough working should be done in this booklet.
Calculators may be used.
[Turn over
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1 Disaccharides are formed following synthesis from monosaccharides or as a result of
polysaccharide hydrolysis.
Cellobiose, maltose, sucrose and trehalose are four different disaccharides found in nature.
A B C D
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2 Which statement is true for phospholipid, but not for protein?
A Its molecules have hydrophilic and hydrophobic components.
B Its molecules are synthesised from non-identical sub-units.
C It is a barrier to water soluble molecules.
D It is found in cell membranes.
3 The diagram shows four different amino acids, each with a different R group (side chain).
Which amino acids could form a hydrophobic interaction between their R groups?
A 1 and 2
B 1 and 3
C 2 and 4
D 3 and 4
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4 Which features affect the tensile strength of collagen?
1 the helical structure of collagen chains
2 the small R group of the amino acids in collagen
3 the insoluble nature of collagen
4 the covalent bonds between collagen molecules
A 1, 2, 3 and 4
B 1 and 3 only
C 1, 2 and 4 only
D 2, 3 and 4 only
5 Four students were asked to match the function with the appearance of some cell structures
in an animal cell.
Which student correctly matched the numbered functions with the appearance of the cell
structure?
1 2 3
A W X Z
B W Z V
C Y W V
D Y Z W
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6 A scientist carried out an experiment to separate cell structures in animal cells.
This extract was filtered into a centrifuge tube and then spun in a centrifuge. The heaviest cell
structure sank to the bottom forming pellet 1, as shown in the diagram.
The liquid above pellet 1 was poured into a clean centrifuge tube and spun in the centrifuge
at a higher speed to separate the next heaviest cell structure. This cell structure sank to the
bottom, forming pellet 2.
This procedure was repeated twice more to obtain pellet 3 and pellet 4, each containing a
single type of cell structure.
Which row shows the order in which the cell structures were collected?
7 The diagram represents part of the fluid mosaic model of a cell surface membrane.
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8 When investigating the rate of reaction of the enzyme lipase on the hydrolysis of triglycerides,
the pH must be maintained at an optimum to prevent the lipase denaturing.
What is the reason for this?
A The addition of water molecules produced by hydrolysis increases pH.
B The addition of water molecules produced by hydrolysis decreases pH.
C The products of hydrolysis decrease the pH.
D The products of hydrolysis increase the pH.
9 A fixed volume of the enzyme catalase was added to a fixed volume of hydrogen peroxide
solution. The diagram shows how the rate of the reaction changed over the course of the
reaction.
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11 The diagram shows part of a nucleic acid.
Which row correctly describes the bonds shown in the diagram at positions 1, 2, 3 and 4?
is easily broken by
can be found in is formed by is formed during
changes in
RNA condensation DNA replication
temperature or pH
A 1, 2 2, 3 1, 2, 3 4
B 3, 4 1 2, 3 2, 3
C 1, 2, 3 1, 2 1, 4 3, 4
D 1 1, 3, 4 1, 2 3, 4
What is the theoretical minimum number of tRNA molecules required to translate the mRNA
for this peptide?
A 4
B 10
C 12
D 30
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13 The diagram shows the classification of several portions of eukaryotic DNA that do not
encode RNA or protein.
V W X Y Z
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14 Recombinant DNA techniques can be used to alter the locations of control elements within
DNA sequences. These techniques allows scientists to study the effects of such location
changes of control elements on the levels of transcription.
The diagram shows the various structures of transcription units and the corresponding
relative levels of transcription.
The promoter, enhancer and coding sequences are represented by letters P, E and G
respectively. The number of symbol ‘+’ indicates the relative frequency of transcription.
15 Each cycle of the polymerase chain reaction (PCR) has three steps.
• DNA denatures to produce separate strands.
• Single-stranded DNA anneals with primers.
• Taq polymerase elongates the primer strands.
Which statements about Taq polymerase are correct?
1 Taq polymerase is not denatured by the temperature of the DNA denaturation
step.
2 Taq polymerase is needed for binding primers to the separated DNA strands.
3 Taq polymerase has a high optimum temperature therefore the temperature can
be raised after annealing for efficient elongation.
4 Taq polymerase does not have to be replaced during each cycle.
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