TDS GM108

anulated
R Gr TM
Technical Data
SS Agar (Salmonella Shigella Agar), Granulated GM108
Intended Use:
SS Agar (Salmonella Shigella Agar), Granulated is a differential selective media used for the isolation of Salmonella and
some Shigella species from pathological specimens, suspected foodstuffs etc.
Composition**
Ingredients Gms / Litre
Peptone 5.000
HM peptone B # 5.000
Lactose 10.000
Bile salts mixture 8.500
Sodium citrate 10.000
Sodium thiosulphate 8.500
Ferric citrate 1.000
Brilliant green 0.00033
Neutral red 0.025
Agar 15.000
Final pH ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
Suspend 63.02 grams in 1000 ml distilled water. Boil with frequent agitation to dissolve the medium completely. DO NOT
AUTOCLAVE OR OVERHEAT. Overheating may destroy selectivity of the medium. Cool to about 50°C. Mix and pour into
sterile Petri plates.
Principle And Interpretation

SS Agar medium is recommended as differential and selective medium for the isolation of Salmonella and Shigella species
from pathological specimens (1) and suspected foodstuffs (2, 3, 4, 5) and for microbial limit test (6). SS Agar is a moderately
selective medium in which gram-positive bacteria are inhibited by bile salts, brilliant green and sodium citrate.
Peptone, HM peptone B provides nitrogen and carbon source, long chain amino acids, vitamins and essential growth
nutrients. Lactose is the fermentable carbohydrate. Brilliant green, bile salts and thiosulphate selectively inhibit gram-positive
and coliform organisms. Sodium thiosulphate is reduced by certain species of enteric organisms to sulphite and H2S gas and
this reductive enzyme process is attributed by thiosulphate reductase. Production of H2S gas is detected as an insoluble black
precipitate of ferrous sulphide, formed upon reaction of H2S with ferric ions or ferric citrate, indicated in the centre of the
colonies.
The high selectivity of Salmonella Shigella Agar allows the use of large inocula directly from faeces, rectal swabs or other
materials suspected of containing pathogenic enteric bacilli. On fermentation of lactose by few lactose-fermenting normal
intestinal flora, acid is produced which is indicated by change of colour from yellow to red by the pH indicator-neutral red.
Thus these organisms grow as red pigmented colonies. Lactose non-fermenting organisms grow as translucent colourless
colonies with or without black centres. Growth of Salmonella species appears as colourless colonies with black centres
resulting from H 2S production. Shigella species also grow as colourless colonies which do not produce H2S.
Type of specimen
Clinical: faeces, blood, rectal swabs ; Suspected food stuffs
Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,8,9).
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (2,3,4,5).
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Warning and Precautions

In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/
eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets.
Limitations
1.The medium is highly selective and may be toxic to certain Salmonella or Shigella species.Hence it is recommended to use
to inoculate plates of less inhibitory media parallel to SS Agar, such as Hektoen Enteric Agar (M467) or Deoxycholate
Citrate Agar (M065) for easier isolation of Shigella species (7).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous granular media
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Reddish orange coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 6.3% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
Organism Growth Inoculum Recovery Colour of
(CFU) colony
Cultural Response
# Klebsiella aerogenes fair 50-100 20-30% cream pink
ATCC 13048 (00175*)
Escherichia coli ATCC fair 50-100 20-30% pink with bile
25922 (00013*) precipitate
Salmonella Choleraesuis good-luxuriant 50-100 >=50% colourless with
ATCC 12011 black centre
Salmonella Typhi ATCC good-luxuriant 50-100 >=50% colourless with
6539 black centre
Enterococcus faecalis ATCC none-poor 50-100 <=10% colourless
29212 (00087*)
Proteus mirabilis ATCC fair-good 50-100 30-40% colourless,
25933 may have black
centre
Shigella flexneri ATCC good 50-100 40-50% colourless
12022 (00126*)
Salmonella Typhimurium good-luxuriant 50-100 >=50% colourless with
ATCC 14028 (00031*) black centre
Salmonella Enteritidis ATCC good-luxuriant 50-100 >=50% colourless with
13076 (00030*) black centre
Key : *Corresponding WDCM numbers. # - Formerly known as Enterobacter aerogenes
Storage and Shelf Life

Store below 30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On
opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the
hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area
protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on
the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
Reference
1.Lennette and others (Eds.), 1985, Manual of Clinical Microbiology, 4th ed., ASM, Washington, D.C.
2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,
APHA, Washington, D.C.
3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,
APHA Inc., Washington, D.C.
4.Eaton A. D., Clesceri L. S., Rice E. W., and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water
and Wastewater, 21st Ed., APHA, Washington, D.C.
5.Williams S., (Ed.), 2005, Official Methods of Analysis of the Association of Official Analytical Chemists, 19th Ed., AOAC,
Washington, D.C.
6.The United States Pharmacopoeia, 2006, USP29/NF24, The United States Pharmacopoeial Convention. Rockville, MD.
7.MacFaddin J., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. I, Williams and
Wilkins, Baltimore.
8. Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
9. Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of
Clinical Microbiology, 11th Edition. Vol. 1.
Revision : 01 / 2018
In vitro diagnostic medical

IVD device
CE Marking
30°C Storage temperature
10°C
Do not use if package is

damaged
HiMedia Laboratories Pvt. Limited,

23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India
EC REP CE Partner 4U ,Esdoornlaan 13, 3951

DB Maarn The Netherlands,
www.cepartner 4u.eu

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office :
A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
techhelp@himedialabs.com Website: www.himedialabs.com

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TDS GM108

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anulated

Principle And Interpretation

Specimen Collection and Handling

Please refer disclaimer Overleaf.

Warning and Precautions

Storage and Shelf Life

In vitro diagnostic medical

30°C Storage temperature

Do not use if package is

HiMedia Laboratories Pvt. Limited,

EC REP CE Partner 4U ,Esdoornlaan 13, 3951

Please refer disclaimer Overleaf.

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