Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests: Biotechnology and Biology
Research Interests:
Stripe rust, also known as yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat production leading to yield losses up to 84%. Due to climate change, new races of the yellow rust pathogen are... more
Stripe rust, also known as yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat production leading to yield losses up to 84%. Due to climate change, new races of the yellow rust pathogen are appearing for which no durable source of resistance has been observed in the present high-yielding varieties. A mutation breeding programme was initiated in two popular varieties, namely PBW343 and HD2967, using gamma-ray and electron beam irradiation. Gamma-ray doses of 250, 300 and 350 Gy and electron beam doses of 150, 200 and 250 Gy were used for seed irradiation. The M2 population was screened in the field from seedling to adult plant stage by spraying a mixture of urediniospores of Pst pathotypes. Disease severity was recorded as the percentage of leaf area covered by the rust pathogen following a modified Cobb's scale. A total of 52 putative yellow rust resistant mutants in HD2967 and 63 in PBW343 were isolated. The number of mutants was higher in the electron beam irradiated population compared with gamma-rays. The absence of sporulation and spore production of the rust pathogen on the mutants indicated resistance. Mutant plants showing seedling resistance also showed resistance at adult plant stage. Seed yield and its contributing characters were better in the mutants compared with the parents. These rust resistant mutants could be novel sources of stripe rust or yellow rust resistance. The plant-to-row progenies of these mutants were confirmed and characterized in the M3 generation.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Transposable elements (TEs) are repetitive deoxyribonucleic acids (DNA) sequences with an ability to change its position within the genome. These TEs make up substantial share ranging from 20% to 85% of plant genome. They are highly... more
Transposable elements (TEs) are repetitive deoxyribonucleic acids (DNA) sequences with an ability to change its position within the genome. These TEs make up substantial share ranging from 20% to 85% of plant genome. They are highly mutagenic and most of them are largely transcriptionally and transpositionally inactive under natural conditions. Environmental stress causes epigenetic de-repression of TEs, which influence on their transcription level resulting in increasing transpositional activity. Plant host genomes have evolved various TE suppression mechanisms, which operate before and after transcription. Transcriptional silencing is achieved through methylation of DNA bases, modifications of histone proteins, and alterations in nucleosome positions. DNA methylation depends largely on RNA-directed DNA methylation (RdDM) pathway. These methylated TEs are preferentially targeted by host genome to recruit the RNA polymerases (PolIV and Pol V) through RdDM for silencing and maintain the methylation marks during DNA replication and cell division. Further, chromatin modifications
Research Interests:
Research Interests: Biotechnology and Biology
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Wheat grain with annual production of 550 million tonnes is the staple food for millions all over the world. In recent times, heat stress has emerged as a major problem in the sub-tropical wheat cultivation areas. Therefore, agronomically... more
Wheat grain with annual production of 550 million tonnes is the staple food for millions all over the world. In recent times, heat stress has emerged as a major problem in the sub-tropical wheat cultivation areas. Therefore, agronomically desirable plant types, with heat stress tolerance have become a necessity. Earlier, significant yield improvement was brought about with the use of GA 3 insensitive dwarfing genes. However, the varieties carrying ‘Norin-10’ genes are sensitive to heat stress and show reduced fertility under high temperature. Short stature varieties grown in Southern Europe, carry a different semidwarfing gene Rht8, which reduces plant height by 10% without significant reduction in yield (Worland et al. 1988). The microsatellite locus Xgwm261 shows polymorphism with predominance of 165bp, 174bp and Abstract
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Abstract In this paper, we derive one-parameter families of Newton, Halley, Chebyshev, Chebyshev-Halley type methods, super-Halley, C-methods, osculating circle and ellipse methods respectively for finding simple zeros of nonlinear... more
Abstract In this paper, we derive one-parameter families of Newton, Halley, Chebyshev, Chebyshev-Halley type methods, super-Halley, C-methods, osculating circle and ellipse methods respectively for finding simple zeros of nonlinear equations, permitting f ′(x)=0 at some ...
Research Interests:
Research Interests:
Wheat is a staple food of Indian population. Its nutritional and quality parameters have gained considerable importance over past few decades. In the present study, genotypic variation was studied in 100 advanced breeding lines developed... more
Wheat is a staple food of Indian population. Its nutritional and quality parameters have gained considerable importance over past few decades. In the present study, genotypic variation was studied in 100 advanced breeding lines developed for Indian peninsular zone by measuring phytic acid (PA), inorganic phosphate (IP), iron and zinc content in seeds and hundred kernel weight (HKW). Advanced breeding lines under investigation exhibited wide variation for the characters studied. The PA content ranged from 4.97 mg/g to 15.02 mg/g (mean of 9.58 mg/g). Iron and zinc content was in the range of 0.042 to 0.098 mg/g and 0.017 mg/g to 0.029 mg/g respectively. HKW ranged from 2.99 to 5.42 g. There was significant negative correlation between PA and HKW. Iron content showed very high genotypic coefficient of variation and heritability (h 2 bs) as compared to zinc content
Research Interests: Biology and Phytic acid
Wheat is a staple food of Indian population. Its nutritional and quality parameters have gained considerable importance over past few decades. In the present study, genotypic variation was studied in 100 advanced breeding lines developed... more
Wheat is a staple food of Indian population. Its nutritional and quality parameters have gained considerable importance over past few decades. In the present study, genotypic variation was studied in 100 advanced breeding lines developed for Indian peninsular zone by measuring phytic acid (PA), inorganic phosphate (IP), iron and zinc content in seeds and hundred kernel weight (HKW). Advanced breeding lines under investigation exhibited wide variation for the characters studied. The PA content ranged from 4.97 mg/g to 15.02 mg/g (mean of 9.58 mg/g). Iron and zinc content was in the range of 0.042 to 0.098 mg/g and 0.017 mg/g to 0.029 mg/g respectively. HKW ranged from 2.99 to 5.42 g. There was significant negative correlation between PA and HKW. Iron content showed very high genotypic coefficient of variation and heritability (h 2 bs) as compared to zinc content and other traits. Low heritability of IP content indicated the environmental influence on the trait.
Research Interests: Biology and Phytic acid
Terminal HS is one of the main bottle-neck in wheat yield and grain-quality. Here, we have developed wheat mutant (M3) for HS-tolerance [parent-MP3054- C-306/CB.SPRING BW/CPAN2072 (Parentage)]. To elucidate the mechanism of... more
Terminal HS is one of the main bottle-neck in wheat yield and grain-quality. Here, we have developed wheat mutant (M3) for HS-tolerance [parent-MP3054- C-306/CB.SPRING BW/CPAN2072 (Parentage)]. To elucidate the mechanism of thermotolerance in mutant, we performed de novo transcriptomic sequencing of mutant (M3), parent (P3), and mutant exposed to HS (M3H). We sequenced 6.5, 7.5, and 7.0 million reads in P3, M3 and M3H and generated 3,05,537 genes and 5,88,788 transcripts with an N50 of 1,349 bp. We observed 6,120 upregulated and 4,428 downregulated transcripts (M3 vs P3), 11,354 upregulated and 12,408 downregulated genes (M3H vs P3) and 4817 upregulated and 9085 downregulated genes (M3H vs M3). Some of the highly upregulated genes observed were HSP20, SOD, ABC transporters, HSF, etc. and downregulated genes were starch synthase, sucrose synthase, etc. Gene Ontology analysis showed ‘ATP-binding’ to be most enriched category. Carbon metabolism pathway was observed most altered under H...
The high_molecular_weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS_PAGE analysis and molecular approaches. SDS_PAGE analysis showed that the 1Ux... more
The high_molecular_weight (HMW) glutenin subunits and their coding genes from Aegilops umbellulata Zhuk. (UU, 2n = 2x = 14) were characterized using SDS_PAGE analysis and molecular approaches. SDS_PAGE analysis showed that the 1Ux subunits from four different accessions possessed electrophoretic mobilities close to, or slower than, that displayed by the 1Dx2.2 subunit of common wheat. The electrophoretic mobilities of the 1Uy subunits were generally similar to those shown by the 1Dy subunits of common wheat. The complete open reading frames of the 1Ux and 1Uy genes were amplified by PCR and subsequently cloned and sequenced. Amino acid sequence comparisons suggested that the primary structure of the 1Ux and 1Uy subunits were identical to that of published HMW glutenin subunits from related species. Phylogenetic analysis indicated that the HMW glutenin subunits of Ae. umbellulata were most closely related to those encoded by the D genome of Triticeae.
Research Interests:
Stripe rust, also known as yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat production leading to yield losses up to 84%. Due to climate change, new races of the yellow rust pathogen are... more
Stripe rust, also known as yellow rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major threat to wheat production leading to yield losses up to 84%. Due to climate change, new races of the yellow rust pathogen are appearing for which no durable source of resistance has been observed in the present high-yielding varieties. A mutation breeding programme was initiated in two popular varieties, namely PBW343 and HD2967, using gamma-ray and electron beam irradiation. Gamma-ray doses of 250, 300 and 350 Gy and electron beam doses of 150, 200 and 250 Gy were used for seed irradiation. The M2 population was screened in the field from seedling to adult plant stage by spraying a mixture of urediniospores of Pst pathotypes. Disease severity was recorded as the percentage of leaf area covered by the rust pathogen following a modified Cobb's scale. A total of 52 putative yellow rust resistant mutants in HD2967 and 63 in PBW343 were isolated. The number of mutants was higher ...
Various biotic and abiotic stresses adversely affect crop yield across the globe. Wheat is a cool-season cereal crop and sensitive to rise in ambient growth temperature. Ninety genotypes were screened under heat stress and non-heat stress... more
Various biotic and abiotic stresses adversely affect crop yield across the globe. Wheat is a cool-season cereal crop and sensitive to rise in ambient growth temperature. Ninety genotypes were screened under heat stress and non-heat stress locations. Low heat susceptibility index of <0.5 for HD2189, NI5643, Kenphad39, HD2501, NI179, N5439, HS240, HD2428 and HD2687 indicated that they are heat tolerant and high susceptibility index of >1.5 for PBN142, NI9947, HD2320, GW190, HD2135, NI917 and Batavia indicated that they are heat susceptible. Based on grain yield and heat susceptibility index, two genotypes namely HD2189 and Batavia with contrasting response to heat stress were selected for the further studies. Their morphological and physiological characterization was carried out at seedling stage using 22°C as control and 33°C as heat stress. Under heat stress conditions, reduction for seedling growth traits, relative water content (RWC) and membrane thermostability (MTS) was le...
Research Interests:
Research Interests:
Research Interests:
Heat stress has an adverse effect on the quality and quantity of agriculturally important crops, especially wheat. The tolerance mechanism has not been explored much in wheat and very few genes/ TFs responsive to heat stress is available... more
Heat stress has an adverse effect on the quality and quantity of agriculturally important crops, especially wheat. The tolerance mechanism has not been explored much in wheat and very few genes/ TFs responsive to heat stress is available on public domain. Here, we identified, cloned and characterized a putative TaHSFA6e TF gene of 1.3 kb from wheat cv. HD2985. We observed an ORF of 368 aa with Hsf DNA binding signature domain in the amino acid sequence. Single copy number of TaHSFA6e was observed integrated in the genome of wheat. Expression analysis of TaHSFA6e under differential HS showed maximum transcripts in wheat cv. Halna (thermotolerant) in response to 38 °C for 2 h during pollination and grain-filling stages, as compared to PBW343, HD2329 and HD2985. Putative target genes of TaHSFA6e (HSP17, HSP70 and HSP90) showed upregulation in response to differential HS (30 & 38 °C, 2 h) during pollination and grain-filling stages. Small HSP17 was observed most triggered in Halna under...