Antonio Ippolito
University of Bari Italy, Soil, Plant and Food Sciences, Faculty Member
Research Interests: Horticulture and Root Rot
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Abstract This paper provides an overview on the primary aspects of preharvest application of biological control agents (microbial antagonists) to reduce postharvest decay on fresh fruits and vegetables. The traits (including environmental... more
Abstract This paper provides an overview on the primary aspects of preharvest application of biological control agents (microbial antagonists) to reduce postharvest decay on fresh fruits and vegetables. The traits (including environmental stress resistance and colonizing ...
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Le prove sono state condotte dal 2011 al 2014 in Valpolicella (provincia di Verona) su terreni fortemente calcarei e ciottolosi. \uc8 stato impiegato il vitigno Corvina allevato a spalliera e con potatura a Guyot. Gli idrolizzati proteici... more
Le prove sono state condotte dal 2011 al 2014 in Valpolicella (provincia di Verona) su terreni fortemente calcarei e ciottolosi. \uc8 stato impiegato il vitigno Corvina allevato a spalliera e con potatura a Guyot. Gli idrolizzati proteici utilizzati erano di origine vegetale (tesi B, tesi C, tesi B + C) e le dosi distribuite per via fogliare sono state di 2 kg/ha (tesi B) e 0,5 kg/ha (tesi C). Le dosi sono state mantenute anche per la miscela dei due prodotti (tesi B + C). Nel 2014 sono stati eseguiti tre trattamenti a partire dalla pre-fioritura (BBCH 57) e distanziati 10 giorni uno dall\u2019altro. I controlli sullo stato idrico delle piante sono stati effettuati avvalendosi di una termocamera a infrarossi (foto 1, 2 e 3) in base al protocollo indicato dalla letteratura sull\u2019utilizzo dell\u2019apparecchiatura. I trattamenti hanno avuto efficacia sui parametri qualitativi della variet\ue0 Corvina. Infatti \ue8 stato osservato un incremento del grado zuccherino delle tesi trattate rispetto al controllo, in un\u2019annata caratterizzata da una seria difficolt\ue0 dell\u2019uva a raggiungere un grado alcolico potenziale soddisfacente. Le differenze sono risultate statisticamente significative. Un altro parametro influenzato dai trattamenti \ue8 stato il contenuto di polifenoli totali della bacca, che \ue8 variato, in modo statisticamente significativo, da 1.322 mg/L del controllo, a 1.733 mg/L della tesi C, a 1.819 della tesi B e a 2.002 mg/L della tesi B + C. Anche il contenuto di antociani totali \ue8 variato positivamente a seguito dei trattamenti effettuati
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In previous trials the flavonoid quercetin proved to be effective in reducing Penicillium expansum infections and patulin accumulation in apples. Since quercetin resulted more effective in in vivo than in in vitro trials, a possible role... more
In previous trials the flavonoid quercetin proved to be effective in reducing Penicillium expansum infections and patulin accumulation in apples. Since quercetin resulted more effective in in vivo than in in vitro trials, a possible role of this substance in enhancing host resistance was hypothesized. To verify this hypothesis, a cDNA library of genes differentially expressed in response to quercetin application was constructed by using the suppression subtractive hybridization (SSH) approach. A total of 89 unique sequences were obtained. By homology search and functional analysis the identified sequences were putatively categorized as belonging to "metabolism", "subcellular localization" and "protein with binding functions or cofactor requirement" classes. Similarity was also found with genes coding proteins whose role in defence mechanisms is still unknown.
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Research Interests: Biology and Alternaria
A survey on Phytophthora spp. in the soils and roots of citrus groves was carried out in the main Syrian growing areas of Lattakia and Tartous. Traditional assays (selective medium with soil dilution plates) were used for pathogen... more
A survey on Phytophthora spp. in the soils and roots of citrus groves was carried out in the main Syrian growing areas of Lattakia and Tartous. Traditional assays (selective medium with soil dilution plates) were used for pathogen detection, and molecular (PCR) assays were used for unanmbiguous identification of P. nicotianae and P. citrophtora in 38.5% of the collected samples. In both locations, P. citrophthora was the predominant species.
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Among 17 salts, calcium chloride (CC) and sodium bicarbonate (SB) were selected as the most effective against Botrytis cinerea in trials on wounded sweet cherries. The combination of CC and SB with Aureobasidium pullulans, strain L47,... more
Among 17 salts, calcium chloride (CC) and sodium bicarbonate (SB) were selected as the most effective against Botrytis cinerea in trials on wounded sweet cherries. The combination of CC and SB with Aureobasidium pullulans, strain L47, reduced Botrytis rot by 98 and 94%, respectively. Tests with postharvest treatments were conducted in 2000 and 2001 using CC, SB, and L47, alone or in combination. In both years, postharvest treatments gave significant reduction of rot incidence compared to the control. The combinations L47+CC and L47+SB were the most effective with a reduction of total rots ranging from 62 to 75%. CC and SB did not show any in vitro toxic effect on A. pullulans and did not modify the natural epiphytic population of yeasts, yeast-like fungi, and filamentous fungi on the fruit surface. In postharvest application, the population of the antagonist was not influenced by the presence of salts.
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... Nine of the isolates most effective in vitro were inoculated in the stem of sour orange seedlings 15 days before pathogen inoculation, in ... Abstract (Italian), Numerosi isolati batterici, ottenuti da tessuti legnosi di piante di... more
... Nine of the isolates most effective in vitro were inoculated in the stem of sour orange seedlings 15 days before pathogen inoculation, in ... Abstract (Italian), Numerosi isolati batterici, ottenuti da tessuti legnosi di piante di limone e di arancio amaro, sono stati saggiati in vitro per l ...
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SIDALC - Servicio de Informacion y Documentacion Agropecuaria de las Americas.
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Table grapes are exposed to fungal infections before and after harvest. In particular, Aspergillus, Penicillium, and Alternaria can cause decays and contamination by mycotoxins. The main fungi affecting Lebanese table grapes after harvest... more
Table grapes are exposed to fungal infections before and after harvest. In particular, Aspergillus, Penicillium, and Alternaria can cause decays and contamination by mycotoxins. The main fungi affecting Lebanese table grapes after harvest were assessed as epiphytic populations, latent infections, and rots. Effects of storage with and without SO2 generating pads were also evaluated. Representative isolates of toxigenic genera were characterised, and their genetic potential to produce ochratoxin A, fumonisins, and patulin was established. The epiphytic populations mainly included wound pathogens (Aspergillus spp. and Penicillium spp.), while latent infections and rots were mostly caused by Botrytis spp. The use of SO2 generating pads reduced the epiphytic populations and rots, but was less effective against latent infections. Characterization of Aspergillus, Penicillium, and Alternaria isolates showed that A. tubingensis, P. glabrum, and A. alternata were the most common species. Stra...
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Abstract Physical treatments have gained great interest in recent years to control many postharvest diseases in fruits and vegetables because the total absence of residues in the treated product and minimal environmental impact. The... more
Abstract Physical treatments have gained great interest in recent years to control many postharvest diseases in fruits and vegetables because the total absence of residues in the treated product and minimal environmental impact. The present review shows the extensive research work conducted during many years and increased in the last 10 years, developing physical means for consistent disease control. The review include the use of cold storage as the main physical method for delaying or reducing biotic and abiotic diseases. Physical treatments, like heat, including hot water and hot air treatments, radio frequency and microwave, hypobaric and hyperbaric pressure and far ultraviolet radiation (UV-C light), are treated as promising control means, and controlled and modified atmospheres as complementary physical tools essential to reduce or delay the development of postharvest pathogens. A particular emphasis is given to the mode of action, which involve direct effect to the pathogen (lethal or sub-lethal) of spore germination and mycelial growth of fungi and the resistance induction in the host which is not well known but nowadays, with the new tools available in molecular biology will be easy to highlight other physiological and biochemical pathways on which the phenomenon are based. Besides benefits of treatment in different commodities, also limitations of use, including low persistence, risk of adverse effects and technological problems for commercial application are discussed.
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Research Interests: Epidemiology and Biology
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A severe olive decline of unknown aetiology is currently under investigation in Apulia, (southern Italy). The decline affects mainly aged trees (100 years or older) growing in the Gallipoli bay, on an area extending about 10.000 ha... more
A severe olive decline of unknown aetiology is currently under investigation in Apulia, (southern Italy). The decline affects mainly aged trees (100 years or older) growing in the Gallipoli bay, on an area extending about 10.000 ha located on the western coast of the Salento peninsula. The disease is characterized by a rapid dieback of shoots, twigs and branches followed by death of the entire tree. Leaf tips and margins turn dark yellow to brown, a condition that spreads inward, eventually leading to desiccation. Symptoms progress in severity from older to younger leaves. Desiccated leaves and mummified drupes remain attached to the shoots. Trunks, branches and twigs viewed in cross section show more or less extensive discolorations of the vascular elements, as reported earlier by Carlucci et al. (2008), plus sapwood and vascular cambium. Numerous galleries of the leopard moth, Zeuzera pyrina and bark beetles occurr on the trunks, branches and twigs of affected plants. Isolations f...