Asam LAKTAT
Asam LAKTAT
Asam LAKTAT
Proses Hulu (upstream proses) Proses Fermentasi Proses Hilir (down stream proses)
SISTEM FERMENTASI
Proses Hulu
Molasses:
Sucrose : 43.8 54.4 Invert sugar: 1.19 4.50 Ash : 5.3 9.60 Total nitrogen : 0.82 1.90 pH 6.40 8.10 PENGHILANGAN LOGAM
PENGENCERAN
molasses: Sucrose : 15 20%
Fe++ + K3(FeCN)6
(agar miring)
A. Niger
Strain:
Inokulum: *sTRAIN
*VOLUME MEDIA=1L
SISTEM FERMENTASI
Proses Fermentasi
Preparasi Media Volume = 9500 L Sukrosa : 12% pH = 6,0
Fermentasi (10.000 L) Sukrosa : 10-12% pH = 6,0 T = 40oC Inokulum = 5% TANPA aerasi Kec. Pengadukan = 50 rpm
KOMPONEN SUKROSA AMMONIUM NITRAT POTASIUM DIHIDROGEN PHOSPAT MAGNISIUM SULPHAT HCL
SISTEM FERMENTASI
Reaksi di Fermentor
Reaksi:
C12H22O11 + H2O + Biomasa C6H12O6
C6H12O6 + Biomasa
C3H6O3 + CaCO3
C3H6O3
C3H4O3 Ca + H2O + CO2
SISTEM FERMENTASI
Keluar Fermentor:
C3H4O3 Ca
Biomasa
C3H4O3 Ca
Pengasaman:
C3H4O3 Ca + H2SO4
C3H6O3 + CaSO4
SISTEM FERMENTASI
Filtrasi CaSO4
(C3H6O3)
SISTEM FERMENTASI