- I was born in a rural of Esfahan district. I am professional in genetic engineering at Shahid Beheshti University of Medical Sciences.edit
Abstract: This study was designed to investigate the relative role of sweetness and comparative effects of different taste sensation of the non-caloric sweetener, aspartame on pain and its interaction with MK-80] as a non-selective MMDA... more
Abstract: This study was designed to investigate the relative role of sweetness and comparative effects of different taste sensation of the non-caloric sweetener, aspartame on pain and its interaction with MK-80] as a non-selective MMDA antagonist by formalin-test in mice. The formalin-test was chosen because it measures the response to a long-lasting nociceptive stimulus and closely resembles to the clinical pain. Morphine induced a dose dependent antinociception in the early and late phases of formalin test. Twelve days ...
<p>Splenocytes were isolated from immunized mice, pooled in each group and assayed for IFN-γ-producing cells by ELISPOT as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045765#s2"... more
<p>Splenocytes were isolated from immunized mice, pooled in each group and assayed for IFN-γ-producing cells by ELISPOT as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045765#s2" target="_blank">Materials and Methods</a>. (A) Data are represented as number of IFN-positive spot forming cells (SFCs) per 10<sup>6</sup> pooled splenocytes in each group ± SD. All samples were tested in triplicate. (B) Frequency of IFN-γ spots in each group of mice.</p
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Background: The protozoan parasites Cryptosporidium spp. and Giardia are known to occur widely in both raw and drinking waters. They are two of the causative agents of waterborne outbreaks of gastroenteritis throughout the world. In the... more
Background: The protozoan parasites Cryptosporidium spp. and Giardia are known to occur widely in both raw and drinking waters. They are two of the causative agents of waterborne outbreaks of gastroenteritis throughout the world. In the present study, a PCR assay and FA were developed for detection of Cryptosporidium oocysts and Giardia cyst in environmental samples. Methods: We have detected Cryptosporidium spp. oocysts and Giardia cysts in seeded and unseeded environmental water samples by PCR method. Water samples were spiked with oocysts (50, 100,300,500) and filtrated with a 1.2-µm pore size cellulose nitrate and follow by DNA extraction and purification by QIAamp DNA mini kit. Nested-PCR assay amplified an 850 bp fragment of 18s rRNA gene specific for Cryptosporidium and 435 bp fragment of glutamate dehydrogenase (GDH) target gene for Giardia. Also many river water from north of Iran, be checked by these methods. Results: Cryptosporidium and Giardia DNAs were detected in seede...
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BACKGROUND: Ovine anaplasmosis and theileriosis are important tick-borne diseases of sheep and goats which are distributed in the tropical and subtropical areas of the world. OBJECTIVES: This study was performed to assess hematological... more
BACKGROUND: Ovine anaplasmosis and theileriosis are important tick-borne diseases of sheep and goats which are distributed in the tropical and subtropical areas of the world. OBJECTIVES: This study was performed to assess hematological status in sheep naturally infected with Anaplasma and Theileria spp. to clarify the pathogenic aspects of various species involved in ovine anaplasmosis and theileriosis in Ahvaz region. METHODS: 109 sheep were sampled, and blood parasite infections were diagnosed by microscopic examination and PCR. The blood samples were also subjected to hematologic assessment. RESULTS: PCR analysis revealed A. ovis infection in 86.2% of sheep, while mixed infections with A. marginale were also detected in 53.2% of them. However, Anaplasma inclusion bodies were only observed in 32.1% of the tested animals. T. ovis were found in 88% of the inspected sheep by PCR, and 67.8% of them were detected microscopically, as well. Hematologic assessment showed that mean RBC, PC...
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<p>CTL activity of vaccinated BALB/c mice against P815 target cells loaded with synthetic p66 peptide was measured using LDH assay. The splenocytes were stimulated <i>in vitro</i> for 72 hours with p66 peptide, the... more
<p>CTL activity of vaccinated BALB/c mice against P815 target cells loaded with synthetic p66 peptide was measured using LDH assay. The splenocytes were stimulated <i>in vitro</i> for 72 hours with p66 peptide, the irrelevant peptide or left un-stimulated and co-cultured with p66-pulsed p815 target cells at different E:T ratios for 6 hours. The effector cells lytic activity was measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049539#s2" target="_blank">Materials and Methods</a>. All data are representative of three independent experiments using pooled spleen cells from five mice and error bars (SD) were calculated based on triplicates. Splenocytes from the mice vaccinated with T7-p66 phage nanoparticles showed a significantly greater cytotoxicity against p66-loaded target cells than the mice receiving p66 emulsified in Freund's adjuvant (p66+FA group). Injection of mice with T7-p66x2 nanoparticles or an emulsion of p66x2 peptide in Freund's adjuvant did not result in a significant cytotoxic activity against target cells compared to negative controls (T7-wt, PBS and CFA/IFA). Similarly, a mixture of T7-wt nanoparticles and p66 peptide only induced a background level of cytotoxic activity in splenocytes similar to negative controls. Data corresponding to T7-wt and PBS have not been shown.</p
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Background: Extraction of blood genomicDNAis one of the main approaches for clinical and molecular biology studies. Although several methods have been developed for extraction of blood genomic DNA, most of these methods consume long time... more
Background: Extraction of blood genomicDNAis one of the main approaches for clinical and molecular biology studies. Although several methods have been developed for extraction of blood genomic DNA, most of these methods consume long time and use expensive chemicals such as proteinase K and toxic organic solvent such as phenol and chloroform. The objective of this study was to developed easy and safe method forDNAextraction from clotted and frozen whole blood. This method has many advantages: time reducing, using inexpensive materials, without phenol and chloroform, achieving of high molecular weight and good quality genomicDNA. M aterials and Methods: DNA extraction was performed by two methods (new and phenol-chloroform method). Then quantity and quality parameters were evaluated by 1% agarose gel electrophoresis, Nano drop analysis and efficiency of Polymerase Chain Reaction (PCR). R es ults: Extracted DNA from 500μL of blood samples were 457.7ng/μl and 212ng/μL and their purity (...
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Rv1733c is a latency antigen from, a probable integral-membrane protein with promiscuous T-cell and B-cell epitopes, making it a potential vaccine candidate against tuberculosis. This study aimed to clone and optimize the expression of... more
Rv1733c is a latency antigen from, a probable integral-membrane protein with promiscuous T-cell and B-cell epitopes, making it a potential vaccine candidate against tuberculosis. This study aimed to clone and optimize the expression of recombinant Rv1733c infor purification. Chemically synthesizedcoding sequence was cloned in pET-23a(+) followed by transformingBL21 (DE3) cells. To evaluate the induction conditions for optimized expression, factorial design of experiments was employed using four different media as well as four levels of isopropyl-b-D-thiogalactopyranosid [IPTG] concentration and duration of induction. The recombinant protein was then purified using a His-tag purification kit and detected through western blotting. Recombinant Rv1733c (> 24 kDa) was expressed and accumulated in the cytoplasm of thecells. Medium composition showed the most significant effect on the yield of the recombinant protein (P = 0.000). The highest yield of recombinant Rv1733c occurred in the ...
Root resorption is a complication of orthodontic treatment and till date, there is a dearth of information regarding this issue. The aim of this study was to determine whether the expression of transforming growth factor-β1 (TGF-β1, an... more
Root resorption is a complication of orthodontic treatment and till date, there is a dearth of information regarding this issue. The aim of this study was to determine whether the expression of transforming growth factor-β1 (TGF-β1, an inflammatory cytokine) is related to orthodontic force. Moreover, if associated, the expression level may be helpful in differential diagnosis, control and ultimate treatment of the disease. In this experimental study, a total of 24 eight-week-old male Wistar rats were selected randomly. On day 0, an orthodontic appliance, which consisted of a closed coil spring, was ligated to the upper right first molar and incisor. The upper left first molar in these animals was not placed under orthodontic force, thus serving as the control group. On day 21, after anesthesia, the animals were sacrificed. The rats were then divided into two equal groups where the first group was subjected to histological evaluation and the second group to reverse transcriptase-poly...
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CD24 (cluster of differentiation 24) is a small heavy glycosylated protein, which is overexpressed in many cancer and some cancer stem cells and is associated with the development, invasion, and metastasis of cancer cells. The exact role... more
CD24 (cluster of differentiation 24) is a small heavy glycosylated protein, which is overexpressed in many cancer and some cancer stem cells and is associated with the development, invasion, and metastasis of cancer cells. The exact role of CD24 in these processes is not fully understood, however, in this article, it has been tried to present collection of cancer-related mechanisms attributed to CD24. Based on the literature, CD24 dis-regulates different signaling pathways in various cancer cells, including; Src kinases, STAT3, EGFR, Wnt/β-catenin and MAPK. Src kinases play an important role in the signaling pathways which activate p38 MAPK and STAT3 pathways. Akt and ERK are downstream effectors of CD24-activated EGFR, which promote cell proliferation, invasion, and metastasis. CD24 increases expression of HER2 by activation of NF-κB transcription factor. Moreover, CD24 up-regulates expression of miR-21 oncomir through activation of Src kinases. Identification of the details of the...
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Background: Cystic hydatidosis disease (CHD) is a common disease of human and animal caused by contamination with the metacestode stage of echinococcus granulosus. The disease causes cyst in tissue body of patients. Antigen B is one of... more
Background: Cystic hydatidosis disease (CHD) is a common disease of human and animal caused by contamination with the metacestode stage of echinococcus granulosus. The disease causes cyst in tissue body of patients. Antigen B is one of the important antigens in liquid cyst. Methods: Expressed and purified recombinant antigen of echinococcus granulosus was used as antigen in ELISA (Enzyme-linked immunosorbent assay) method. The handmade kit was studied with physical and bacteriostatical methods. Physical stability was assessed with accelerated stability test (Arrhenius equation). First, numeral negative and positive samples were evaluated; then, the kit was exposed in special temperatures and times; afterwards, the samples were evaluated again and compared with the first evaluation. Proclin300, Soduim azide and Bacillus subtilis were chosen in bacteriostatic method. Different concentration of bacillus and different percents of preservatives were added to solutions of the kit. Growth of bacteria was evaluated and the least percent of preservatives that had the most effect of bacteriostatic was determinated. Findings: According to stability test, the handmade kit had one-year stability and do not have stability for two years in the 2-8ºC tempreture. Sodium azide had destructive effect on solutions of the kit; but, proclin300 with the concentration of 0.05% was appropriate for solutions. Conclusion: Stability and bacteriostatic methods showed that the handmade kit can be maintained in 2-8ºC with significant activity.
Research Interests: Chemistry, Biomedical Engineering, Drug delivery, Transmission Electron Microscopy, Scanning Transmission Electron Microscopy, and 15 moreMagnetic Resonance Spectroscopy, Medicine, Humans, Differential scanning calorimetry, Mice, Animals, PEGylation, In Vivo, Fourier transform infrared spectroscopy, Neoplasms, Drug Carriers, Cell Survival, Gemcitabine, Polyethylene Glycols, and Nanotubes Carbon
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هصلاخ فده و هقباس : دنتسه ناكدوك رد عياش تلاكشم زا يكي ينادند ياه هسبآ . يلـصا تلع ناونعب اه مسيناگراوركيم يرـپ و پـلاپ يراـميب سانوموريفروپ ياه هنوگ هب قلاعتم هايس نامگيپ هدننك ديلوت يفنم مرگ يزاوه يب ياه مسيناگرا اهنآ نيب رد هك دنا هدش... more
هصلاخ فده و هقباس : دنتسه ناكدوك رد عياش تلاكشم زا يكي ينادند ياه هسبآ . يلـصا تلع ناونعب اه مسيناگراوركيم يرـپ و پـلاپ يراـميب سانوموريفروپ ياه هنوگ هب قلاعتم هايس نامگيپ هدننك ديلوت يفنم مرگ يزاوه يب ياه مسيناگرا اهنآ نيب رد هك دنا هدش هتخانش رلاوكيدار لاتو يرپ و ) اهديئورتكاب ( نك يـم يزاـب رلاوكيدار يرپ و پلاپ يراميب يكينيلك ياه هناشن و مئلاع داجيا رد ار يمهم شقن دـن . نـيا اذـل هـعلاطم يـسررب فدـه اـب نژوتاـپ مسـيناگراوركيم ود روضـح prevotella melaninogenica و porphyromonas gingivalis رد رد يريش ياه نادند كينژوتندا ياه هسبآ 40 كدوك 10 4 يكشزپ مولع هاگشناد يكشزپنادند هدكشناد لافطا شخب هب هدننك هعجارم هلاس ت لاس رد يتشهب ديهش يليصح 79 1378 ماجنا تفرگ . اهشور و داوم : دوب هداس يريگ هنوـمن شور و يهاگشيامزآ يسررب عون زا رضاح قيقحت شور . ميب ـ يراميب هنوگچيه هدش باختنا نارا دندوب هدركن تفايرد كيتويب يتنآ ،يريگ هنومن زا لبق هتفه ود لقادح و هتشادن كيمتسيس . ي لقادح هدش باختنا ناراميب هيلك نادند ك باس اب هارمه يكينيلك هدهاشم لباق هسبآ هب لاتبم ـ خا ياهزور يط درد هق ـ دنتشاد ري . كينكت زا Polymerase Chain Reaction (PCR) □ يكشزپنادند مولع تاقيقحت زكرم بوصم يتاقيقحت حرط * رگ رايشناد يتشهب ديهش يكشزپ مولع هاگشناد ،يكشزپنادند هدكشناد ،ناكدوك يكشزپنادند هو ** لباب يكشزپ مولع هاگشناد ،يكشزپنادند هدكشناد ،ناكدوك يكشزپنادند هورگ رايداتسا *** يتشهب ديهش يكشزپ مولع هاگشناد ،يكشزپ هدكشناد ،يسانشبوركيم و يژولونوميا هورگ رايشناد Assessment of pathogens microorganisms of periapical abscess in primary teeth
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Page 1. ﻲﻜﺷﺰﭘرد ﺶﻫوﮋﭘ ) ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ﻲﺸﻫوﮋﭘ ﻪﻠﺠﻣ ( ﻲﺘﺸﻬﺑ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد هرود 34 هرﺎﻤﺷ ، 1 ، رﺎﻬﺑ 1389 ، تﺎﺤﻔﺻ 20 ﺎﺗ 25 ﮓﻨﻴﻧﻮﻠﻛ cDNA رﻮﺘﻛﺎﻓ VII ﺎﻣﻮﺗﺎﭙﻫ ﻲﻟﻮﻠﺳ هدر زا ﻞﺻﺎﺣ يدﺎﻘﻌﻧا ﻲﻧﺎﺒﻌﺷ ﺎﺴﻳﺮﭘ 1 رﻮﭘ هﺪﻨﺑ... more
Page 1. ﻲﻜﺷﺰﭘرد ﺶﻫوﮋﭘ ) ﻲﻜﺷﺰﭘ هﺪﻜﺸﻧاد ﻲﺸﻫوﮋﭘ ﻪﻠﺠﻣ ( ﻲﺘﺸﻬﺑ ﺪﻴﻬﺷ ﻲﻧﺎﻣرد ﻲﺘﺷاﺪﻬﺑ تﺎﻣﺪﺧ و ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد هرود 34 هرﺎﻤﺷ ، 1 ، رﺎﻬﺑ 1389 ، تﺎﺤﻔﺻ 20 ﺎﺗ 25 ﮓﻨﻴﻧﻮﻠﻛ cDNA رﻮﺘﻛﺎﻓ VII ﺎﻣﻮﺗﺎﭙﻫ ﻲﻟﻮﻠﺳ هدر زا ﻞﺻﺎﺣ يدﺎﻘﻌﻧا ﻲﻧﺎﺒﻌﺷ ﺎﺴﻳﺮﭘ 1 رﻮﭘ هﺪﻨﺑ نﺎﮔﮋﻣ ، 2 رﻮﭘ ﻲﻠﻘﻣﺎﻣا ﻪﻟﻼﺳ ، 1 ﻦﻏﺎﻣ ﻼﻴﻟ ، 2 ﺪﻴﺳ رﺎﮕﻧ ، 2 ﻲﻳﺎﻤﻐﻳ ماﺮﻬﺑ ، 1 ، ماﺮﻬﺑ ﻲﻤﻇﺎﻛ *2 ،3 ...
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Over many years, variable gene expression systems have been used obtaining of Factor VII protein, but each one has certain limitations. Therefore, the main goal of this study was to assess the biological activity of purified and... more
Over many years, variable gene expression systems have been used obtaining of Factor VII protein, but each one has certain limitations. Therefore, the main goal of this study was to assess the biological activity of purified and recombinant factor VII expressed in Iranian lizard Leishmania. After transferring recombinant construct containing FVII gene to Leishmania, first, the expression of 55 kDa FVII protein in transfected cells was confirmed by analyzing cell lysate using SDS-PAGE and Western Blotting techniques. Then, FVII was purified by NI-NTA-His Tag- resin through an affinity chromatography. The chromogenic activity of human recombinant Factor VII (amidolytic activity) in supernatant and pellet fractions of Leishmania promastigotes was done using ELISA method. The amidolytic activity of rhFVII was about 0.0125 IU/ml in the concentrated cell sediment and 0 IU/ml in the supernatant in the first 60 minutes and after that time, none of the samples showed acceptable activity. HIG...
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Vibrio fischeri is a symbiotic marine bacterium and a nonpathogenic member of the Vibrionaceae which produces luminescence by expressing the lux operon. The lux operon encoding luciferase ( luxAB ) and proteins required to synthesize the... more
Vibrio fischeri is a symbiotic marine bacterium and a nonpathogenic member of the Vibrionaceae which produces luminescence by expressing the lux operon. The lux operon encoding luciferase ( luxAB ) and proteins required to synthesize the aldehyde substrate (luxCDE ), is controlled with luxR and luxI . In this study, we amplified the chromosomal fragment contains luxAB of V. fischeri , amplified fragment cloned into the pTZ57R vector and sequencing to confirmed the fragment. The sub cloning of luxAB gene was carried out in the pETDuet-1 expression vector and expression procedures were performed in Escherichia coli strain Nova blue. As a result, a 2046 bp fragment which contains the whole fragment of luciferase coding genes and intergenic sequences were cloned in pETDuet-1 expression vector. pETDuet luxAB recombinant plasmid was confirmed by restriction analysis; subsequently 76 kD expressed protein was detected using SDS–PAGE and western blot using specific polyclonal antibody. In th...
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Background: Infections are one of the correctable causes of infertility with low cost and cost effective treatment. The 50% of infertile cases is related to men in some way, and 30% of them are absolutely related to them. Mycoplasmas are... more
Background: Infections are one of the correctable causes of infertility with low cost and cost effective treatment. The 50% of infertile cases is related to men in some way, and 30% of them are absolutely related to them. Mycoplasmas are the smallest microorganisms with capability of DNA replication. Present study is planned to compare the mycoplasma infection in infertile men and men with established fertility. M aterials and Methods: 45 Semen samples were collected from case and control persons who reffered to Royan Infertility and Fertility Institute between 2004 and 2005 and stored in -20°C until time of test. DNA was extracted from semen using phenol chloroform . PCR reaction was done by mycoplasma specific primers. R es ults: Mycoplasma genitalium gene was amplified in 6 (40%) cases from 15 infertile semen samples and 11 (36.6%) from 30 control semen samples. C onclusion: Probability of genital infection, at least, in these studies group, is very lower than other communities&#...
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Silencing of gene expression by siRNA (small interfering RNA) is a powerful approach used to study the genetic analysis and functional roles of mammalian genes. There is at present no report about the effects of mammalian two-hybrid... more
Silencing of gene expression by siRNA (small interfering RNA) is a powerful approach used to study the genetic analysis and functional roles of mammalian genes. There is at present no report about the effects of mammalian two-hybrid system plasmids delivery of sense and antisense strands. The leishmania pteridine reductase 1 (PTR1) gene was cloned as sense and antisense strands into mammalian two hybrid system plasmids. The constructs were transfected into human blood macrophages on the basis of eight experimental groups. (Antisense strand ± LPS, sense strand ± LPS, dsRNA ± LPS, negative control ± LPS). After 24 hours, cytokines production was assessed with ELISA. Transfection of sense and antisense strand RNA into monocyte-derived macrophages (MDM) was confirmed by RT-PCR. Single strands RNA expressed IL-8, IL-12, IL-1β inflammatory cytokines and dsRNA induced IL-8, IL-12 and TNF-α production in MDM. In contrast, random uptake from a mixture of two plasmids was downregulated IL-8, ...
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Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of TNF family, is an interesting ligand which selectively induces apoptosis in tumor cells and, therefore, it has been developed for cancer therapy. This ligand has... more
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a member of TNF family, is an interesting ligand which selectively induces apoptosis in tumor cells and, therefore, it has been developed for cancer therapy. This ligand has been produced by various hosts such as E.coli. However, protein expression in E.coli cytoplasm leads to problems such as incorrect folding, reduction in biological activity, inclusion body formation, and sophisticated downstream. The aim of this study is to develop an expression system for the production of recombinant TRAIL secreted to the E.coli periplasm instead of cytoplasm. By using Overlapping Extension PCR, an OmpA signal sequence was fused to TRAIL cDNA and OmpA-TRAIL fragment was then cloned in pET-22b plasmid. This construct was confirmed by PCR and DNA sequencing. Promoter was induced in E.coli BL21 (DE3) and periplasmic expressed proteins were released using osmotic shock procedure. SDS-PAGE analysis showed that about 37% of recombinant...
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Background: Gene therapy is one of the treatment method for diabetes mellitus. An insulin gene, under control of heat shock inducer promoter was used in the study.Materials and Methods: Six mice used in this study. Streptozotocin (STZ)... more
Background: Gene therapy is one of the treatment method for diabetes mellitus. An insulin gene, under control of heat shock inducer promoter was used in the study.Materials and Methods: Six mice used in this study. Streptozotocin (STZ) induced diabetes mellitus in BALB/C mice and rats. Recombinant plasmid was injected to each animal. Animal’s blood sugar (BS) was measured. Warming was done at injection site with a hair dryer to induced gene expression.Results: Immediately after warming blood sugar was increased in mouse 1 and decreased after one hour. However, blood sugar increased again in mice 2 and 4. Blood sugar increased for two hours after warming in mouse 3. Blood sugar increased to 150 mg/dl after STZ injection and immediately after warming reached to 160 mg/dl, after one hour BS dropped to 116 mg/dl and on the second hour was 126 mg/dl, in mouse 5 BS had an increasing trend, BS in mouse 6 had a similar pattern to mouse 2.Conclusion: With fixing the defects of the project wi...
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Background: Cystic echinococcosis can cause severe disease and probable death in humans. Epitopes of its antigens play a key role in the sensitivity and specificity of immunodiagnostic tests. Methods: Epitope prediction software programs... more
Background: Cystic echinococcosis can cause severe disease and probable death in humans. Epitopes of its antigens play a key role in the sensitivity and specificity of immunodiagnostic tests. Methods: Epitope prediction software programs predict the most antigenic linear B-cell epitopes of AgB (8 kD), Ag5, and Ag95. Six such epitopes were predicted and connected by “Gly-Ser” linker and synthesized. The purity of the concentrated recombinant multi-epitope protein was assessed by 15% SDS-PAGE. Overall, 186 serum samples were collected from the Loghman Hakim Hospital and different laboratories, Tehran, Iran, from July 2016 to February 2017. Patients infected with hepatic hydatid cysts, patients infected by other parasites and viruses, and healthy individuals were used to detect the anti-CE IgG using recombinant multi-epitope protein. Results: Forty-one samples out of 43 cases of hydatidosis were diagnosed correctly as positive, and two were negative. In addition, six negative cases of ...
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Polytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several... more
Polytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several immunogenic epitopes, inducing immune responses. In this study, a DNA construct comprising of sequences encoding epitopes from SAG1, 2 and 3 was designed and cloned into pET28a expression vector and subsequently expressed and purified, using Ni-NTA column. The SDS-PAGE and Western blotting analysis showed that polytopic genes were successfully expressed and purified. The surface antigenic protein of can be applied in the future epitope-based applications.
Trichinellosis is an important and neglected foodborne zoonotic infectious disease in worldwide. The most human outbreaks in recent years have been related to consumption of wild boar meat. This cross-sectional study determined the... more
Trichinellosis is an important and neglected foodborne zoonotic infectious disease in worldwide. The most human outbreaks in recent years have been related to consumption of wild boar meat. This cross-sectional study determined the prevalence of Trichinella spp. infections in hunted wild boars in northern Iran. Thirty-five hunted wild boars were subjected in this study in 2015. All samples were examined by conventional artificial digestion method to detect of muscle larvae. Genomic DNA was extracted by phenol-chloroform method from isolated larvae. To identify the Trichinella species, a PCR-based method was applied using the internal transcribed spacer 2 (ITS2) and mitochondrial small-subunit ribosomal RNA (rRNA) gene sequences. The overall prevalence of Trichinella spp. infection was 5.7% (2/35, 95%CI= 0-13.4). The mean larval burdens in two positive samples were 0.05 and 6 larvae per gr tissue muscle, respectively. The PCR reaction, using specific primers, yielded two 367 bp and 1...
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Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. Chondrogenic differentiation of iPSCs is important for application in cartilage tissue... more
Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. Chondrogenic differentiation of iPSCs is important for application in cartilage tissue engineering. In this study, we considered the effect of nanofibre-based polyethersulfone (PES) scaffold on the chondrogenesis of iPSCs. IPSC cells were cultured on the PES scaffold and scaffold free method. After 21 d, real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. For confirm our results, we have done immunocytochemistry and scanning electron microscopy (SEM) imaging. According to the results, higher significant expressions of common chondrogenic-related genes such as aggrecan, collagen type II and collagen type X were observed in PES seeded human iPSCs when compared to the mRNA levels measured in scaffold free method. Expression of collagen type I down regulated in both methods. Also, both methods were sh...
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Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for... more
Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. The gene encoding ESAT-6 was cloned into pET22b(+) plasmid and expressed in Escherichia coli BL21 (DE3). The purified recombinant ESAT-6 protein was injected into female BALB/c mice for immunization, and then m-RNA was extracted from the spleen of immunized mice. The anti-ESAT-6 VH/k chain library was assembled by joining of VH and k into the VH/k chain with a 72-bp DNA linker by SOE (splicing by overlap extension) PCR. The scFv library was panned against ESAT-6 using a single round of ribosome display via a rabbit reticulocyte lysate system. ELISA assay showed that one of the selected scFvs had higher affinity against the recombinant...
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We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran. Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously... more
We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran. Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp. based on morphological and metric assessment of external features of fresh adults, morphological and metric assessment of internal anatomy of stained mounted worms. Statistical analysis was conducted using the Student's t-test implemented in SPSS 15.0 (SPSS, Chicago, Illinois). Then the morphometric criteria of Fasciola samples were compared with PCR-RFLP data. The results of PCR-RFLP were confirmed by COI gene sequence. The differences between the body length, area of the body, peripheral of the body, succer area, cone length, cone width, in two species were significant (P < 0.05). Based on Morphological ...
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The current study was designed to evaluate immune responses induced by DNA vaccines encoding 8-kDa subunit of antigen B (HydI) of Echinococcus granulosus and murine interleukin 12 (IL-12) as genetic adjuvants in BALB/c mice. Expression... more
The current study was designed to evaluate immune responses induced by DNA vaccines encoding 8-kDa subunit of antigen B (HydI) of Echinococcus granulosus and murine interleukin 12 (IL-12) as genetic adjuvants in BALB/c mice. Expression plasmid pcDNA3.1 containing HydI (pcHyd1) as vaccine along with the murine interleukin 12 (pcMIL12) as adjuvant were used. Thirty-five mice in the five experimental groups received PBS, empty pcDNA3.1, pcHydІ, pcMIL-12, and pcHydІ+ pcMIL-12 in days zero, 14(th) and 28(th). Two weeks after the last immunization, evaluation of the immune response was performed by evaluating the proliferation of splenic lymphocytes, IFN-γ and IL-4, determination of IgG isotyping titer. Mice that received the pcHydI+pcMIL12 exhibited higher levels of lymphocyte proliferation compared to mice that received the pcHydI alone (P<0.001), and produced significantly more IFN-γ in comparison to other groups (P< 0.001). In addition, they produced significantly less IL-4 than...
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Haptoglobin (Hp) is a plasma α2-sialoglycoprotein that contains alpha and beta chains. It displays in three common phenotypes, Hp1-1, Hp2-1, and Hp2-2. Proteins expressed by polymorphic genes have grossly different molecular sizes... more
Haptoglobin (Hp) is a plasma α2-sialoglycoprotein that contains alpha and beta chains. It displays in three common phenotypes, Hp1-1, Hp2-1, and Hp2-2. Proteins expressed by polymorphic genes have grossly different molecular sizes resulting in different diffusion rates in the brain. Haptoglobin expressed by the Hp2-2 genotype has lower hemoglobin-binding capacity than Hp1-1 or Hp2-1 and is associated with idiopathic generalized epilepsy. To determine polymorphism in haptoglobin genes in patients with idiopathic generalized tonic-clonic seizures, 42 men, 42 women, and 50 controls were selected for this study. Genomic DNA was extracted from blood and studied by polymerase chain reactions (PCR). The amplified fragments for the Hp1-1 and Hp2-2 genotypes were 1757 and 3481 base pairs (bp) respectively, and the Hp2-1 genotype had both fragments, in addition to a 349-bp fragment. The distribution of the three major Hp phenotypes in epilepsy patients was 28.6 (1-1), 38.1 (2-1), and 33.3% (2...
Research Interests: Medicine and Haptoglobin
Neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. The factor H binding protein (fHBP) is a key virulence factor of Neisseria meningitidis that is able to selectively bind to human factor H, the key regulator of... more
Neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. The factor H binding protein (fHBP) is a key virulence factor of Neisseria meningitidis that is able to selectively bind to human factor H, the key regulator of the alternative complement pathway, which it has important implications for meningococcal pathogenesis and vaccine design. The aims of present research were cloning, expression, purification of fHbp and confirmation of the interaction between serum factor H (fH) and produced factor H binding protein. A 820 base pairs fhbp gene fragment was amplified by PCR and cloned into expression vector pET28a (+) in Bam HI and SalI restriction enzymes sites. Recombinant DNA was expressed in BL21 (DE3) cell. fHBP protein was purified by Ni-NTA agarose resin. Coupling of recombinant protein into CNBr activated Sepharose 4B resin was carried out for application in serum fH protein purification. (fH-fHBP) interaction was confirmed by SDS-PAGE and far-western blotti...
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Patients with diabetes mellitus type II suffer from hyperglycemia because they are not able to use the insulin that they produce, often due to inadequate function of insulin receptors. There are some evidences that this deficiency is... more
Patients with diabetes mellitus type II suffer from hyperglycemia because they are not able to use the insulin that they produce, often due to inadequate function of insulin receptors. There are some evidences that this deficiency is inherited in a dominant autosomal manner and leads to the malfunction of the pancreatic beta cells resulting in insulin excretion disorders. In this study, we sought to identify mutations in the insulin receptor (INSR) gene, which can cause insulin resistance in type II diabetic patients. DNA was extracted from peripheral blood cells of the patients (n = 128) diagnosed with type II diabetes. All 22 exons of the INSR gene of the patients were analyzed for mutations running PCR, conformation-sensitive gel electrophoresis and DNA sequencing, consecutively. Approximately 26% of the patients had genetic mutations; however, most of them were not reported. These mutations include exon 2 (His171Asn, Ile172Ser, Cys196Ser and Ser210Arg), exon 3 (Gly227Asp and Gly...
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Background: The homologous recombination (HR) is one of the conventional cloning methods for the production of recombinant DNA. It is a quick method for in vivo DNA cloning without using the high cost restriction enzymes. A few... more
Background: The homologous recombination (HR) is one of the conventional cloning methods for the production of recombinant DNA. It is a quick method for in vivo DNA cloning without using the high cost restriction enzymes. A few modifications in the cloning procedure can increase the efficiency of this method. M aterials and Methods: In this study, effect of heating on the rate of the IgG1 heavy chain gene cloning was investigated in the HR method and then it was compared with HR method without heating and traditional cloning method. For doing this comparison, three cloning methods including HR, HR with the heat treatment, and traditional cloning were used to clone the human IgG1 heavy chain into the pcDNA3.1(+) plasmid. R es ults: The results showed that adding heat in the HR method converts insert and vector from the double strand DNA to the single strand DNA. This allows them to copulate with each other better and faster than the two other methods. The heat addition also helps the...