The actual frequency of occurrence of particular beer-spoiling bacteria in different beer types and in different areas of the beer production chain is reported in this investigation of 150 samples from the ongoing production of a south... more
The actual frequency of occurrence of particular beer-spoiling bacteria in different beer types and in different areas of the beer production chain is reported in this investigation of 150 samples from the ongoing production of a south German brewery. The recently approved rapid detection system, VIT-Bier plus L. brevis, was used for the detection of beer-spoiling bacteria. This study reveals that the composition of beer types, especially with regard to the level of beer haze as measured in EBC units, has a large impact on the sensitivity of beer to contamination with beer-spoiling bacteria. In particular, the beers with a low level of haze showed higher contamination rates than did the beers with a high level of haze. The study confirms that Lactobacillus brevis is the most frequently occurring beer-spoiling bacterium, regardless of the type of beer. It led to spoilage in all analyzed beer types. Other beer-spoiling bacteria were only detected in the beer type helles, which interestingly contains a low level of haze (18 EBC units). Beer-spoiling bacteria were found in many areas throughout the production process. However, contamination occurred most frequently within the yeast propagation, filtration room, bright beer cellar, and filling area. All other sampled production stages were free of contamination. These results show that weak spots in the production process can be located and that they need to be regularly controlled by using fast and reliable detection systems in order to prevent the spread of beer-spoiling bacteria.
Research Interests: Food Science, Biology, Evaluation, Analysis, Quality Assurance, and 7 moreBacteria, Haze, Gene, Contamination, Gen, Analisis, and food spoilage
The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among... more
The Bacteria community composition in an acidic Sphagnum peat bog (pH 3.9 to 4.5) was characterized by a combination of 16S rRNA gene clone library analysis, rRNA-targeted fluorescence in situ hybridization (FISH), and cultivation. Among 84 environmental 16S rRNA gene clones, a set of only 16 cloned sequences was closely related (≥95% similarity) to taxonomically described organisms. Main groups of clones were affiliated with the Acidobacteria (24 clones), Alphaproteobacteria (20), Verrucomicrobia (13), Actinobacteria (8), Deltaproteobacteria (4), Chloroflexi (3), and Planctomycetes (3). The proportion of cells that hybridized with oligonucleotide probes specific for members of the domains Bacteria (EUB338-mix) and Archaea (ARCH915 and ARC344) accounted for only 12 to 22% of the total cell counts. Up to 24% of the EUB338-positive cells could be assigned by FISH to specific bacterial phyla. Alphaproteobacteria and Planctomycetes were the most numerous bacterial groups (up to 1.3 × 10...
Research Interests: Biology, Activated Sludge, Medicine, Gene Cloning, Fluorescence in situ hybridization, and 15 moreApplied, Applied Environmental Microbiology, Bacteria, Community Structure, Bog, Gram Positive Bacteria, Greenhouse Gas, Bacterial Diversity, Ecosystem, Fluorescent in situ hybridization, Industrial wastewater, Carbon Budget, Low Temperature, Culture Media, and Community Composition
Pattern of radiation-induced RET and NTRK1 rearrage-ments in 191 post-Chernobyl papillary thyroid carci-nomas: biological, phenotypic and clinical implication // Clin Cancer Res.-2000.-№ 6.-Р. 1093-1103. 19. Ciampi R., Knauf J.A., Kerier... more
Pattern of radiation-induced RET and NTRK1 rearrage-ments in 191 post-Chernobyl papillary thyroid carci-nomas: biological, phenotypic and clinical implication // Clin Cancer Res.-2000.-№ 6.-Р. 1093-1103. 19. Ciampi R., Knauf J.A., Kerier R. et al. Onco-genic AKFP0-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer // J Clin Invest.-2005.-№ 115.-Р. 94-101. 20. Kumagai A., Namba H., Saenko V.A. et al. Low frequency of BRAFT1796A mutations in childhood thyroid carcinomas // J Clin. Endocrinol. Metab.-2004.-№ 89.-Р. 4280-4284. 21. Lima J., Trovisco V., Soares P. et al. BRAF mutations are not a major event in post-Chernobyl childhood thyroid carcinomas // J Clin. Endocrinol. Metab.-2004.-№ 89.-Р. 4267-4271. 22. Detours V., Wattel S., Venet D. et al. Absence of a specific radiation signature in post-Chernobyl thyroid cancers // British J Cancer.-2005.-№ 92.-Р. 1545-1552. 23. Rogounovitch T.I., Saenko V.A., Ashizawa K., Sedliarou I.A., Namba H. et al. TP53 codon ...
Research Interests: Biology, Population Dynamics, Medicine, Multidisciplinary, Potato, and 11 moreWastewater, Fluorescence in situ hybridization, Textile industry, Waste Water Treatment Plant, Water Science and Technology, Fluorescent In-Situ Hybridisation, Operant Conditioning, Industrial Waste, Industrial wastewater, Sewage Treatment, and Proteobacteria
The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome... more
The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome sequences of Symbioflor2 E. coli components contained a number of virulence-associated genes. Their presence seems to be in conflict with a recorded history of safe use, and with the observed low frequency of adverse effects over a period of more than 6 years. The genome sequences were used to identify unique sequences for each component, for which strain-specific hybridization probes were designed. A colonization study was conducted whereby five volunteers were exposed to an exceptionally high single dose. The results showed that the probiotic E. coli could be detected for 3 months or longer in their stools, and this was in particular the case for those components containing higher numbers of virulence-associated genes. Adverse effects from this long...
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Research Interests:
The traditional phenotypic identification of LAB is rather tedious and not always reliable. Nucleic acid probe technology may be an alternative for a faster and more reliable differentiation. 16S or 23S rRNA-targeted oligonucleotides have... more
The traditional phenotypic identification of LAB is rather tedious and not always reliable. Nucleic acid probe technology may be an alternative for a faster and more reliable differentiation. 16S or 23S rRNA-targeted oligonucleotides have been used for the specific identification of ...
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Research Interests:
Research Interests: Analytical Chemistry, Biomedical Engineering, Fluorescence, Biology, Nanotechnology, and 15 moreMedicine, Bioaerosols, FISH, Bacteria, ribosomal RNA, Actinomycetales, Reproducibility of Results, Large Scale, Cell Wall, Sensor system, Sensitivity and Specificity, Biosensing Techniques, Air Pollutants, Molecular Probe, and Air Microbiology
Research Interests:
Molecular genetic aberrations and the related phenotypes were investigated in 191 papillary thyroid carcinomas (PTCs) from patients exposed at young age to radioiodine released from the Chernobyl reactor. A high prevalence of RET gene... more
Molecular genetic aberrations and the related phenotypes were investigated in 191 papillary thyroid carcinomas (PTCs) from patients exposed at young age to radioiodine released from the Chernobyl reactor. A high prevalence of RET gene rearrangements (62.3%) with a significant predominance of ELE1/RET (PTC3) over H4/RET (PTC1) rearrangements was found in PTCs of the first post-Chernobyl decade. NTRK1 rearrangements were rare (3.3%). In 3.3%, we observed novel types of RET rearrangements: GOLGA5/ RET (PTC5), HTIF/RET (PTC6), RFG7/RET (PTC7), and an as yet undefined RFGX/RET.RET rearrangements, preferentially ELE1/RET, are related to rapid tumor development. At longer intervals after exposure to ionizing radiation, the prevalence of RET rearrangements declines with a shift from ELE1/RET to H4/RET, most significantly in female patients. The prevalence of specific types of rearrangements is independent of age at irradiation. A significantly higher prevalence of ELE1/RET was observed in t...
Research Interests:
Molecular genetic aberrations and the related phenotypes were investigated in 191 papillary thyroid carcinomas (PTCs) from patients exposed at young age to radioiodine released from the Chernobyl reactor. A high prevalence of RET gene... more
Molecular genetic aberrations and the related phenotypes were investigated in 191 papillary thyroid carcinomas (PTCs) from patients exposed at young age to radioiodine released from the Chernobyl reactor. A high prevalence of RET gene rearrangements (62.3%) with a significant predominance of ELE1/RET (PTC3) over H4/RET (PTC1) rearrangements was found in PTCs of the first post-Chernobyl decade. NTRK1 rearrangements were rare (3.3%). In 3.3%, we observed novel types of RET rearrangements: GOLGA5/ RET (PTC5), HTIF/RET (PTC6), RFG7/RET (PTC7), and an as yet undefined RFGX/RET.RET rearrangements, preferentially ELE1/RET, are related to rapid tumor development. At longer intervals after exposure to ionizing radiation, the prevalence of RET rearrangements declines with a shift from ELE1/RET to H4/RET, most significantly in female patients. The prevalence of specific types of rearrangements is independent of age at irradiation. A significantly higher prevalence of ELE1/RET was observed in t...
Research Interests:
Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the... more
Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the prerequisites for its biological (biocatalytic) stability. In this paper, growth of biofilm comprising ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the open cellular polyurethane material polyurethane sponge bioreactor has been investigated.Dynamics of the biofilm formation was estimated using AOB and NOB metabolic activity and the volume occupied by these two types of bacteria in the biofilm. Spectrophotometry liquid ion chromatography and image cytometry were used, respectively, for these measurements. A mathematical model of the dynamics of biofilm formation was established. These data indicate that open cellular polyurethane material is a good basis for the immobilization of nitrifying bacteria. Moreover, growth of the ...
Research Interests: Biofilms, Kinetics, Environmental Monitoring, Ion Chromatography, Multidisciplinary, and 13 moreFISH, Fluorescence in situ hybridization, Bacterial Toxins, Bacteria, System Development, Mathematical Model, Polyurethanes, Nitrite Oxidizing Bacteria, Bioreactors, Water Microbiology, Quaternary Ammonium Compounds, Catalytic Activity, and Nitrites
ABSTRACT
The ability of probiotic Escherichia coli to colonise the human gut was determined in a volunteer study following national (German) regulations. Five persons voluntarily took a single, high dose of Symbioflor®2, which contains 6 different... more
The ability of probiotic Escherichia coli to colonise the human gut was determined in a volunteer study following national (German) regulations. Five persons voluntarily took a single, high dose of Symbioflor®2, which contains 6 different probiotic E. coli genotypes, to assess tolerance of the product, after which presence of E. coli in their faeces was tested for a follow-up period of 30 weeks. Intake of the product did not result in severe side effect in any of the individuals, though mild side effects were observed. Stool analysis showed that the probiotic E. coli had colonised all five persons for a period of 10 to 30 weeks (mean: 18.7 weeks, median: 25.7 weeks). In two individuals there was evidence of competition between host E. coli and probiotic E. coli, while in two others total E. coli levels increased persistently with at least a factor of 10 as a result of the received dose. In one individual, who had lacked detectable levels of faecal E. coli at the start of the post-au...
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Conventional cultivation methods and molecular approaches were utilised to describe the filamentous bacterial population of industrial activated sludge WWTPs. In total 43 strains were isolated by micromanipulation and were affiliated with... more
Conventional cultivation methods and molecular approaches were utilised to describe the filamentous bacterial population of industrial activated sludge WWTPs. In total 43 strains were isolated by micromanipulation and were affiliated with 12 different species, comprising two new species and a new genus. In particular, a new species of Microthrix, a new genus of a filamentous Alphaproteobacteria morphologically similar to Nostocoida limicola, and a new filamentous species closely related to the opportunistic pathogen Propionibacterium propionicum were obtained. Despite the high number of isolates, the cultivation approach was unable to describe the filamentous bacteria most common in industrial WWTP. A culture-independent approach, termed the cell sorting/RT-PCR method, was therefore applied to identify fastidious or non-culturable filamentous microrganisms from different industrial plants. By this method the relevant filaments were micromanipulated and their 16S rDNA genes were ampl...
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Research Interests:
... 216-221 (1997) © Gustav Fischer Verlag Rapid Genotypic Differentiation of Lactococcus lactis Subspecies and Biovar CLAUDIA BEIMFOHR, WOLFGANG LUDWIG and ... and are listed in Table 3. All Lactococcus and Streptococcus strains were... more
... 216-221 (1997) © Gustav Fischer Verlag Rapid Genotypic Differentiation of Lactococcus lactis Subspecies and Biovar CLAUDIA BEIMFOHR, WOLFGANG LUDWIG and ... and are listed in Table 3. All Lactococcus and Streptococcus strains were cultured in M17 broth containing 9 ...
Research Interests: Biofilms, Kinetics, Environmental Monitoring, Ion Chromatography, Multidisciplinary, and 13 moreFISH, Fluorescence in situ hybridization, Bacterial Toxins, Bacteria, System Development, Mathematical Model, Polyurethanes, Nitrite Oxidizing Bacteria, Bioreactors, Water Microbiology, Quaternary Ammonium Compounds, Catalytic Activity, and Nitrites
Research Interests: Evolutionary Biology, Genetics, Microbiology, Carbon, Medical Microbiology, and 15 moreActivated Sludge, Multidisciplinary, Biological Sciences, Bacteria, Microbial Mat, Phylogenetic analysis, Functional diversity, Phenotype, Bacillus subtilis, Organic Waste, Lactic Acid, Geologic Sediments, New Taxa, Molecular Sequence Data, and Azospirillum
Research Interests: Evolutionary Biology, Biodiversity, Fluorescence in situ hybridization, Applied, Animals, and 15 moreApplied Environmental Microbiology, Bacteria, Cluster Analysis, Community Structure, Ectomycorrhizal fungi, Fagus Sylvatica, Fluorescent in situ hybridization, Clone, Gastrointestinal Tract, Ascomycota, Cytophaga, Ectomycorrhiza, Full Length Movies, DNA fragmentation, and Direct sequence
The phylogeny and distribution of filamentous Alphaproteobacteria, morphologically similar to... more
The phylogeny and distribution of filamentous Alphaproteobacteria, morphologically similar to "Nostocoida limicola" and Eikelboom Type 021N that cause the solids separation problem of bulking in industrial activated sludge plants is described here. A combination of culture-dependent and culture-independent molecular methods has characterized 5 novel species. 16S rRNA targeted oligonucleotide probes were designed for their in situ identification by fluorescence in situ hybridisation (FISH) and used to monitor their presence in 86 WWTPs treating different industrial effluents in four European countries. The involvement of these bacteria in bulking in these plants was confirmed. Filaments hybridising with the ALF-968 probe for the Alphaproteobacteria were present in 65% of the WWTPs examined. They were dominant and therefore probably responsible for bulking in 25.5% of them. The heterogeneous filamentous alphaproteobacterial populations in these communities could be completely identified after application of the oligonucleotide probes used in this study in 91% of the plants containing them. The only filamentous Alphaproteobacteria retrieved in pure culture was isolated from three different industrial WWTPs plants. None of these isolates could grow anaerobically on glucose or denitrify, but all grew aerobically and heterotrophically on a range of carbon sources. Although morphologically similar to the Eikelboom Type 021N morphotype, they were not involved in sulphur metabolism. These bacteria accumulated lipidic storage granules that were associated with their presence under the unbalanced growth conditions existing in these plants.
Research Interests: Evolutionary Biology, Microbiology, Microscopy, Activated Sludge, In Situ Hybridization, and 13 morePhylogeny, Europe, Lipid metabolism, Alphaproteobacteria, Fluorescent in situ hybridization, Carbohydrate metabolism, Sewage, Industrial wastewater, RT PCR, Water Microbiology, Ribosomal DNA, Sulfur Compounds, and Molecular Sequence Data
Research Interests: Analytical Chemistry, Biomedical Engineering, Fluorescence, Nanotechnology, Bioaerosols, and 15 moreFISH, Fluorescence in situ hybridization, Bacteria, ribosomal RNA, Actinomycetales, Fluorescent In-Situ Hybridisation, Reproducibility of Results, Large Scale, Species Specificity, Cell Wall, Sensor system, Sensitivity and Specificity, Biosensing Techniques, Air Pollutants, and Molecular Probe
The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome... more
The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome sequences of Symbioflor2 E. coli components contained a number of virulence-associated genes. Their presence seems to be in conflict with a recorded history of safe use, and with the observed low frequency of adverse effects over a period of more than 6 years. The genome sequences were used to identify unique sequences for each component, for which strain-specific hybridization probes were designed. A colonization study was conducted whereby five volunteers were exposed to an exceptionally high single dose. The results showed that the probiotic E. coli could be detected for 3 months or longer in their stools, and this was in particular the case for those components containing higher numbers of virulence-associated genes. Adverse effects from this long-term colonization were absent. Thus, the presence of the identified virulence genes does not result in a pathogenic phenotype in the genetic background of these probiotic E. coli.
Research Interests:
Molecular genetic aberrations and the related phenotypes were investigated in 191 papillary thyroid carcinomas (PTCs) from patients exposed at young age to radioiodine released from the Chernobyl reactor. A high prevalence of RET gene... more
Molecular genetic aberrations and the related phenotypes were investigated in 191 papillary thyroid carcinomas (PTCs) from patients exposed at young age to radioiodine released from the Chernobyl reactor. A high prevalence of RET gene rearrangements (62.3%) with a significant predominance of ELE1/RET (PTC3) over H4/RET (PTC1) rearrangements was found in PTCs of the first post-Chernobyl decade. NTRK1 rearrangements were rare (3.3%). In 3.3%, we observed novel types of RET rearrangements: GOLGA5/ RET (PTC5), HTIF/RET (PTC6), RFG7/RET (PTC7), and an as yet undefined RFGX/RET.RET rearrangements, preferentially ELE1/RET, are related to rapid tumor development. At longer intervals after exposure to ionizing radiation, the prevalence of RET rearrangements declines with a shift from ELE1/RET to H4/RET, most significantly in female patients. The prevalence of specific types of rearrangements is independent of age at irradiation. A significantly higher prevalence of ELE1/RET was observed in t...
Research Interests:
Research Interests:
The prevalence of NTRK1 re-arrangement was determined in papillary thyroid carcinomas (PTCs) of children from Belarus who had been exposed to radioactive iodine after the Chernobyl reactor accident; 81 tumors were included, all of which... more
The prevalence of NTRK1 re-arrangement was determined in papillary thyroid carcinomas (PTCs) of children from Belarus who had been exposed to radioactive iodine after the Chernobyl reactor accident; 81 tumors were included, all of which were devoid of RET re-arrangement as analyzed in a current study on genomic alterations in PTC. Oncogenic fusion of the NTRK1 tyrosine kinase domain with the amino-terminal part of the tropomyosin gene (TPM3/NTRK1, trk) was observed in 5 tumors. A single tumor exhibited a TPR/NTRK1 fusion (TRK-T2). Reciprocal NTRK1/TPM3 transcripts were found in 4 of 5 tumors with TPM3/NTRK1 re-arrangement, indicating an intra-chromosomal balanced reciprocal inversion. No phenotypic differences from other post-Chernobyl childhood PTCs were detected. As compared with the high prevalence of RET re-arrangements reported for thyroid carcinomas of children after the Chernobyl reactor accident, NTRK1 re-arrangements appear rare. Our results confirm that activation of receptor tyrosine kinase genes plays the predominant role in post-Chernobyl childhood thyroid carcinogenesis.
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Research Interests:
Research Interests:
The diversity of filamentous bacteria present in industrial wastewater treatment plants was analysed by a combination of classical and molecular-biological approaches. Many unknown filamentous bacteria were observed in about 80 screened... more
The diversity of filamentous bacteria present in industrial wastewater treatment plants was analysed by a combination of classical and molecular-biological approaches. Many unknown filamentous bacteria were observed in about 80 screened activated sludge samples from different ...