Research Interests:
Research Interests: Microbiology, Phytopathology, Plant Biology, Biology, Biocontrol of Plant Pathogens, and 14 morePlant diseases, Biocontrol, Host-parasite interactions, Pathogen, Plant Roots, Fungal Biology, Microbial Interactions, Time Factors, Chitinase, Ascomycota, Glycosyl Hydrolases, Gene expression profiling, Gossypium, and real time polymerase chain reaction
... Yautepec, Morelos, México. CP 62731;Michelina Ruocco, Stefania Lanzuise, Sheridan Woo,Matteo Lorito. ... Trichoderma antagonism against M. phaseolina has been previously reported as a micoparasitism which reduces phytopathogen... more
... Yautepec, Morelos, México. CP 62731;Michelina Ruocco, Stefania Lanzuise, Sheridan Woo,Matteo Lorito. ... Trichoderma antagonism against M. phaseolina has been previously reported as a micoparasitism which reduces phytopathogen survival (Baird et al., 2003). ...
Research Interests:
Research Interests:
Research Interests: Geography, Epidemiology, Phytopathology, Pepper, Virus, and 5 moreMedicina, Incidence, Revista, Growth rate, and Capsicum annuum
ABSTRACT We evaluated the ability of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg to induce resistance to the fungal plant pathogen, Sclerotium rolfsii Saccardo, in three onion (Allium cepa L.) varieties. Both the... more
ABSTRACT We evaluated the ability of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg to induce resistance to the fungal plant pathogen, Sclerotium rolfsii Saccardo, in three onion (Allium cepa L.) varieties. Both the severity of disease and the activities of glucanase, chitinase and peroxidase (enzymes involved in plant resistance) were evaluated in onions inoculated with T. asperellum alone, S. rolfsii alone, or both T. asperellum and S. rolfsii (dual-inoculation) and compared to uninoculated (control) plants. In dual inoculations, the presence of T. asperellum reduced the severity of disease symptoms caused by S. rolfsii. Inoculation with T. asperellum alone increased glucanase, chitinase and peroxidase activity in bulbs, roots and leaves of all three onion varieties compared to uninoculated controls; bulbs of the variety Red Satan (RS) had the highest enzyme activity. In plants inoculated with S. rolfsii alone, enzyme activity was only increased in bulbs and roots compared to uninoculated controls. The highest levels of enzyme activity also occurred only in bulbs and roots of plants that had been dual-inoculated with T. asperellum and S. rolfsii. Plants of the RS variety showed the highest enzyme activities (both constitutive and induced) and showed the lowest severity of disease. Therefore, application of T. asperellum has potential as a biological control alternative to synthetic fungicides for protection of onion crops against infection by S. rolfsii. This protection depends on both constitutive and induced defence responses and varies amongst onion varieties.
Research Interests:
Research Interests:
Three Candida oleophila strains (L06, L07 smooth, and L07 rough) were evaluated in vivo and in vitro as biocontrol agents against Penicillium expansum on postharvest ‘Golden Delicious’ apples (Malus domestica Borkh.) in Chihuahua, Mexico.... more
Three Candida oleophila strains (L06, L07 smooth, and L07 rough) were evaluated in vivo and in vitro as biocontrol agents against Penicillium expansum on postharvest ‘Golden Delicious’ apples (Malus domestica Borkh.) in Chihuahua, Mexico. The in vivo and in vitro activity of exo-β-1,3-glucanase was measured as a possible biocontrol mode of action for C. oleophila. Mean disease incidence caused by P. expansum was 0.3% for apples treated with fludioxonil + ciprodinil, which were used as a positive control, and 1% for fruits treated with a combination of the three C. oleophila strains; the effects of these treatments were significantly equivalent. Disease incidence in control apples was 39% and was significantly different from the other treatments. The in vivo exo-β-1,3-glucanase activity began at 24 h and peaked at 72 and 96 h for all treatments. Strain L06 had the highest activity (7.96 nKat) and a specific activity of 2.92 nKat μg-1. Candida albicans had the lowest activity (2.83 nKat) and a specific activity of 0.67 nKat μg-1. The highest in vitro activity was for C. albicans (85.03 nKat) and the lowest for strain L06 (78.2 nKat). Significant differences in both in vivo and in vitro enzymatic activity were observed between strain L06 and C. albicans. Polynomial regression analysis (R2 = 0.96 in vitro and 0.68 in vivo) indicated that increased enzymatic activity was associated with reduced fruit disease incidence. The production of exo-β-1,3-glucanase by C. oleophila is a possible mode of action for the efficient biocontrol of P. expansum on postharvest apples.