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Calmodulins (CaMs) are the most ubiquitous calcium sensors in eukaryotes. A number of CaM-binding proteins have been identified through classical methods, and many proteins have been predicted to bind CaMs based on their structural... more
Calmodulins (CaMs) are the most ubiquitous calcium sensors in eukaryotes. A number of CaM-binding proteins have been identified through classical methods, and many proteins have been predicted to bind CaMs based on their structural homology with known targets. However, multicellular organisms typically contain many CaM-like (CML) proteins, and a global identification of their targets and specificity of interaction is lacking. In an effort to develop a platform for large-scale analysis of proteins in plants we have developed a protein microarray and used it to study the global analysis of CaM/CML interactions. An Arabidopsis thaliana expression collection containing 1,133 ORFs was generated and used to produce proteins with an optimized medium-throughput plant-based expression system. Protein microarrays were prepared and screened with several CaMs/CMLs. A large number of previously known and novel CaM/CML targets were identified, including transcription factors, receptor and intrace...
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1 Abstract A graph optimization problem arising from topological construction of optimal interconnection networks is the embedding of an interconnection graph of n nodes in a graph of m > n nodes. Interconnection networks are graphs... more
1 Abstract A graph optimization problem arising from topological construction of optimal interconnection networks is the embedding of an interconnection graph of n nodes in a graph of m > n nodes. Interconnection networks are graphs with low diameter (logarithmic with the number of vertices), and sublogarithmic degree, which make them efficient for distributed indexing applications. These networks are used to connect parallel computing system components. Another application is the construction of communication networks with a deBruijn graph structure, where the mapping of data objects to nodes is given by a hash function. To route or look-up data from the network, the address of data items is first obtained from the hash table. Network nodes route the request for data objects or packets addressed to distributed objects such that to minimize some network metric (e.g. network load, delay). The optimal topology construction can be formulated as an embedding of an interconnection net...
Current VR telerehabilitation systems use offline remote monitoring from the clinic and patient-therapist videoconferencing. Such "store and forward" and video-based systems cannot implement medical services involving patient... more
Current VR telerehabilitation systems use offline remote monitoring from the clinic and patient-therapist videoconferencing. Such "store and forward" and video-based systems cannot implement medical services involving patient therapist direct interaction. Real-time telerehabilitation applications (including remote therapy) can be developed using a shared Virtual Environment (VE) architecture. We developed a two-user shared VE for hand telerehabilitation. Each site has a telerehabilitation workstation with a videocamera and a Rutgers Master II (RMII) force feedback glove. Each user can control a virtual hand and interact hapticly with virtual objects. Simulated physical interactions between therapist and patient are implemented using hand force feedback. The therapist's graphic interface contains several virtual panels, which allow control over the rehabilitation process. These controls start a videoconferencing session, collect patient data, or apply therapy. Several e...
Research Interests: Telecommunications, Software Design, Library and Information Studies, Humans, Computer Simulation, and 13 moreFeedback, Local Area Networks, Real Time, Physical Interaction, Microcomputers, Computer User Interface Design, Public health systems and services research, Force Feedback, Remote Monitoring, Remote Consultation, Graphical User Interface, Video Recording, and Commercial Off-The-Shelf
Nonhost resistance (NHR) is the most prevalent form of plant immunity. In Arabidopsis, NHR requires membrane-localized ATP-binding cassette (ABC) transporter PENETRATION (PEN) 3. Upon perception of pathogen-associated molecular patterns,... more
Nonhost resistance (NHR) is the most prevalent form of plant immunity. In Arabidopsis, NHR requires membrane-localized ATP-binding cassette (ABC) transporter PENETRATION (PEN) 3. Upon perception of pathogen-associated molecular patterns, PEN3 becomes phosphorylated, suggestive of PEN3 regulation by post-translational modification. Here, we investigated the PEN3 protein interaction network. We probed the Arabidopsis protein microarray AtPMA-5000 with the N-terminal cytoplasmic domain of PEN3. Several of the proteins identified to interact with PEN3 in vitro represent cellular Ca(2+) sensors, including calmodulin (CaM) 3, CaM7 and several CaM-like proteins, pointing to the importance of Ca(2+) sensing to PEN3-mediated NHR. We demonstrated co-localization of PEN3 and CaM7, and we confirmed PEN3-CaM interaction in vitro and in vivo by PEN3 pull-down with CaM Sepharose, CaM overlay assay and bimolecular fluorescence complementation. We also show that just like in pen3, NHR to the nonadapted fungal pathogens Phakopsora pachyrhizi and Blumeria graminis f.sp. hordei is compromised in the Arabidopsis cam7 and pen3 cam7 mutants. Our study discloses CaM7 as a PEN3-interacting protein crucial to Arabidopsis NHR and emphasizes the importance of Ca(2+) sensing to plant immunity.
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Page 1. An Architecture for QoS Data Replication in Network Virtual Environments George V. Popescu and Christopher F. Codella IBM TJ Watson Research Center {popescu, codella}@ us.ibm.com Abstract Predicted availability ...
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A PC-based orthopedic rehabilitation system was developed for use at home, while allowing for remote monitoring from the clinic. The home rehabilitation station has a Pentium II PC with graphics accelerator, Polhemus tracker, and a novel... more
A PC-based orthopedic rehabilitation system was developed for use at home, while allowing for remote monitoring from the clinic. The home rehabilitation station has a Pentium II PC with graphics accelerator, Polhemus tracker, and a novel Multipurpose Haptic Control Interface with its own Pentium board. This interface is used to sample patient's hand positions and to provide resistive forces using the Rutgers Master II (RMII) glove. A library of virtual rehabilitation routines was developed using WorldToolKit software. At the present time, it consists of two physical therapy exercises (DigiKey and Ball) and two functional rehabilitation exercises (Peg Board test and Ball game). All VR exercises allow automatic and transparent patient data collection into an Oracle database. A remote Pentium II PC is connected with the home-based PC over the Internet and an additional video-conferencing connection. The remote computer running Oracle server is used to maintain the patient database,...
Research Interests: Computer Graphics, Telemedicine, Exercise therapy, Physical Therapy, Video Conferencing, and 10 moreSoftware, Library and Information Studies, Humans, Haptic Interface Design, Data Collection, Microcomputers, Computer User Interface Design, Public health systems and services research, Force Feedback, and Remote Monitoring
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In this paper we present two analog networks that implement an algorithm for motion detection in image sequences. The time consuming stochastic or suboptimal deterministic relaxation algorithms used by the initial Markov Random Field... more
In this paper we present two analog networks that implement an algorithm for motion detection in image sequences. The time consuming stochastic or suboptimal deterministic relaxation algorithms used by the initial Markov Random Field based model are replaced by the energy minimization of the analog network to its state of minimal power dissipation. We describe the elementary cell derived from the model equation and the network architectures. Possible application are motion remote control and motion coding at video rate.
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APA PsycNET Our Apologies! - The following features are not available with your current Browser configuration. - alerts user that their session is about to expire - display, print, save, export, and email selected records - get My ...
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Page 1. Network overlays for efficient control of large scale dynamic groups George V. Popescu, Zhen Liu george.popescu@acm.org, zhenl@ us.ibm.com Abstract Scalable data distribution in large-scale dynamic collaborative ...
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The problem of word recognition in databases of han dwritten documents is addressed. The particular approach taken exploits t he natural line-oriented structure present in handwritten material, which fa cilitates the search and allows the... more
The problem of word recognition in databases of han dwritten documents is addressed. The particular approach taken exploits t he natural line-oriented structure present in handwritten material, which fa cilitates the search and allows the utilization of the temporal information embedded in the data. The proposed method is based on elastic matching between features extracted from the keywords, provided as
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User 1 This paper1 presents a multimodal interface fea- turing fusion of multiple modalities for natural human-computer interaction. The architecture of the interface and the methods applied are de- scribed, and the results of the... more
User 1 This paper1 presents a multimodal interface fea- turing fusion of multiple modalities for natural human-computer interaction. The architecture of the interface and the methods applied are de- scribed, and the results of the real-time mul- timodal fusion are analyzed. The research in progress concerning a mission planning scenario is discussed and other possible future directions are also presented.
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Salicylic acid (SA) is a small phenolic molecule with hormonal properties, and is an essential component of the immune response. SA exerts its functions by interacting with protein targets; however, the specific cellular components... more
Salicylic acid (SA) is a small phenolic molecule with hormonal properties, and is an essential component of the immune response. SA exerts its functions by interacting with protein targets; however, the specific cellular components modulated by SA and critical for immune signal transduction are largely unknown. To uncover cellular activities targeted by SA, we probed Arabidopsis protein microarrays with a functional analog of SA. We demonstrate that thimet oligopeptidases (TOPs) constitute a class of SA-binding enzymes. Biochemical evidence demonstrated that SA interacts with TOPs and inhibits their peptidase activities to various degrees both in vitro and in plant extracts. Functional characterization of mutants with altered TOP expression indicated that TOP1 and TOP2 mediate SA-dependent signaling and are necessary for the immune response to avirulent pathogens. Our results support a model whereby TOP1 and TOP2 act in separate pathways to modulate SA-mediated cellular processes.
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Receptors localized at the plasma membrane are critical for the recognition of pathogens. The molecular determinants that regulate receptor transport to the plasma membrane are poorly understood. In a screen for proteins that interact... more
Receptors localized at the plasma membrane are critical for the recognition of pathogens. The molecular determinants that regulate receptor transport to the plasma membrane are poorly understood. In a screen for proteins that interact with the FLAGELIN-SENSITIVE2 (FLS2) receptor using Arabidopsis thaliana protein microarrays, we identified the reticulon-like protein RTNLB1. We showed that FLS2 interacts in vivo with both RTNLB1 and its homolog RTNLB2 and that a Ser-rich region in the N-terminal tail of RTNLB1 is critical for the interaction with FLS2. Transgenic plants that lack RTNLB1 and RTNLB2 (rtnlb1 rtnlb2) or overexpress RTNLB1 (RTNLB1ox) exhibit reduced activation of FLS2-dependent signaling and increased susceptibility to pathogens. In both rtnlb1 rtnlb2 and RTNLB1ox, FLS2 accumulation at the plasma membrane was significantly affected compared with the wild type. Transient overexpression of RTNLB1 led to FLS2 retention in the endoplasmic reticulum (ER) and affected FLS2 glyc...
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Research Interests: Polymorphism, Multidisciplinary, Humans, Gene Duplication, Gene Dosage, and 12 morePhysical chromosome mapping, High Resolution, Human Genome, Copy Number Variation, Phenotypic variation, Genetic Polymorphism, Base Sequence, DNA Copy Number, DNA sequence, Molecular Sequence Data, High density, and Chromosome aberrations
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The results of some previous work describing a method for fast and precise recovery of the parameters of a signal from a few samples are discussed and extended. The method introduces the representation of the signal in the space of its... more
The results of some previous work describing a method for fast and precise recovery of the parameters of a signal from a few samples are discussed and extended. The method introduces the representation of the signal in the space of its samples and, through it, it ...
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Protein kinase–driven phosphorylation constitutes the core of cellular signaling. Kinase components of signal transduction pathways are often targeted for inactivation by pathogens. The study of kinases and immune signal transduction in... more
Protein kinase–driven phosphorylation constitutes the core of cellular signaling. Kinase components of signal transduction pathways are often targeted for inactivation by pathogens. The study of kinases and immune signal transduction in the model crop tomato (Solanum lycopersicum) would benefit from the availability of community-wide resources for large scale and systems-level experimentation. Here, we defined the tomato kinome and performed a comprehensive comparative analysis of the tomato kinome and 15 other plant species. We constructed a tomato kinase library (TOKN 1.0) of over 300 full-length open reading frames (ORF) cloned into a recombination-based vector. We developed a high-throughput pipeline to isolate and transform tomato protoplasts. A subset of the TOKN 1.0 library kinases were expressed in planta, were purified, and were used to generate a functional tomato protein microarray. All resources created were utilized to test known and novel associations between tomato ki...