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    monika dalal

    Twenty-four serovars of Bacillus thuringiensis (Bt) were screened by polymerase chain reaction to detect the presence of vegetative insecticidal protein gene (vip)-like sequences by using vip3Aa1-specific primers. vip-like gene sequences... more
    Twenty-four serovars of Bacillus thuringiensis (Bt) were screened by polymerase chain reaction to detect the presence of vegetative insecticidal protein gene (vip)-like sequences by using vip3Aa1-specific primers. vip-like gene sequences were identified in eight serovars. These genes were cloned and sequenced. The deduced amino acid sequence of the vip3Aa14 gene from Bacillus thuringiensis tolworthi showed considerable differences as compared to those of Vips reported so far. The vip3Aa14 gene from Bt tolwarthi was expressed in Escherichia coli using expression vector pET29a. The expressed Vip3Aa14 protein was found in cytosolic supernatant as well as pellet fraction, but the protein was more abundant in the cytosolic supernatant fraction. Both full-length and truncated (devoid of signal sequence) Vips were highly toxic to the larvae of Spodoptera litura and Plutella xylostella. Truncation of Vip3Aa14 protein at N-terminus did not affect its insecticidal activity.
    The leaves of necrotic hybrid of wheat (Triticum aestivum L.) exhibited high superoxide content associated with increased lipid peroxidation and membrane damage in earlier studies (Khanna-Chopra et al. 1998, Biochem Biophys Res Commun... more
    The leaves of necrotic hybrid of wheat (Triticum aestivum L.) exhibited high superoxide content associated with increased lipid peroxidation and membrane damage in earlier studies (Khanna-Chopra et al. 1998, Biochem Biophys Res Commun 248: 712-715; Dalal and Khanna-Chopra 1999, Biochem Biophys Res Commun 262: 109-112). In the present study, we investigated the activities of the antioxidant enzymes in the leaves of necrotic wheat hybrids, KalyansonaxC306 (KxC) and WL711xC306 (WLxC) and their parents at different developmental stages. The KxC hybrid exhibited more severe necrosis than WLxC. In KxC, superoxide dismutase (SOD) activity showed no increase over the parents, while WLxC showed an early increase, but it was possibly insufficient to scavenge increased superoxide. Activities of guaiacol peroxidase, ascorbate peroxidase and glutathione reductase were enhanced, while catalase exhibited a decrease in activity, with the appearance of visible necrosis in both the hybrids. The isozy...
    ABSTRACT Sorghum is the one of the most important crops gown on the dryland in India and around the globe. It has the capacity to tolerate and adapt to stress conditions in both tropical and temperate climatic conditions. Sorghum is the... more
    ABSTRACT Sorghum is the one of the most important crops gown on the dryland in India and around the globe. It has the capacity to tolerate and adapt to stress conditions in both tropical and temperate climatic conditions. Sorghum is the versatile crop and has potentials to be utilized as alternate energy crop in view of rising petroleum prices. The process of producing bioethanol the sorghum including sweet and high biomass sorghum is described in this review. Sorghum being high biomass producer had potential to be used for production of bioethanol from lignocellulosic biomass than any other dryland cereals. The importance of sorghum as bioenergy feedstock and bioconversion steps involved in the producing bioethanol is discussed in this paper.
    ... Page 6. 64 Nain et al. ... Planta 217: 367-373. Trontin JF, Harvengt L, Garin E, Lopez-Vernaza MLV, Arancio L, Hoebeke J, Canlet F, and Paques M (2002) Towards genetic engineering of maritime pine (Pinus pinaster Ait.) Ann Forest Sci... more
    ... Page 6. 64 Nain et al. ... Planta 217: 367-373. Trontin JF, Harvengt L, Garin E, Lopez-Vernaza MLV, Arancio L, Hoebeke J, Canlet F, and Paques M (2002) Towards genetic engineering of maritime pine (Pinus pinaster Ait.) Ann Forest Sci 59: 687-697.
    ABSTRACT Sorghum with its remarkable adaptability to drought and high temperature provides a model system for grass genomics and resource for gene discovery especially for abiotic stress tolerance. Group 3 LEA genes from barley and rice... more
    ABSTRACT Sorghum with its remarkable adaptability to drought and high temperature provides a model system for grass genomics and resource for gene discovery especially for abiotic stress tolerance. Group 3 LEA genes from barley and rice have been shown to play crucial role in abiotic stress tolerance. Here, we present a genome-wide analysis of LEA3 genes in sorghum. We identified four genes encoding LEA3 proteins in the sorghum genome and further classified them into LEA3A and LEA3B subgroups based on the conservation of LEA3 specific motifs. Further, expression pattern of these genes were analyzed in seeds during development and vegetative tissues under abiotic stresses. SbLEA3A group genes showed expression at early stage of seed development and increased significantly at maturity, while SbLEA3B group genes expressed only in matured seeds. Expression of SbLEA3 genes in response to abiotic stresses such as soil moisture deficit (drought), osmotic, salt, and temperature stresses, and exogenous ABA treatments was also studied in the leaves of 2-weeks-old seedlings. ABA and drought induced the expression of all LEA3 genes, while cold and heat stress induced none of them. Promoter analysis revealed the presence of multiple ABRE core cis-elements and a few low temperature response (LTRE)/drought responsive (DRE) cis-elements. This study suggests non-redundant function of LEA3 genes in seed development and stress tolerance in sorghum.
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    Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic... more
    Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2 and 3 LEAs among the nine different groups. The present study evaluates the functional role of a Group 4 LEA protein, LEA4-1 from Brassica napus. Expression analysis revealed that abscisic acid, salt, cold and osmotic stresses induce expression of LEA4-1 gene in leaf tissues in Brassica species. Conversely, reproductive tissues such as flowers and developing seeds showed constitutive expression of LEA4, which was up-regulated in flowers under salt stress. For functional evaluation of LEA4-1 with regard to stress tolerance, LEA4-1 cDNA was cloned from B. napus, and overexpressed in both Escherichia coli and transgenic Arabidopsis plants. Overexpression of BnLEA4-1 cDNA in E. coli conferred salt and extreme temperature tolerance to the transformed cells. Furthermore, transgenic Arabidopsis plants overexpressing BnLEA4-1 either under constitutive CaMV35S or abiotic stress inducible RD29A promoter showed enhanced tolerance to salt and drought stresses. These results demonstrate that LEA4-1 plays a crucial role in abiotic stress tolerance during vegetative stage of plant development.