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daniel cruz

    daniel cruz

    ABSTRACT
    This work describes an experimental study of a Cu2ZnSnS4 (CZTS) single phase thin films and powders. The new synthesis method yielded a significant amount of this compound in few minutes (around 3 min) and the growth is based on a layer... more
    This work describes an experimental study of a Cu2ZnSnS4 (CZTS) single phase thin films and powders. The new synthesis method yielded a significant amount of this compound in few minutes (around 3 min) and the growth is based on a layer by layer from a solution of nanocrystal inks and a fast heating by the use of microwaves. The size, structural and morphological properties of the Cu2ZnSnS4 (CZTS) nanoparticles thin films were investigated by using X-ray diffraction (XRD), Raman spectroscopy, FTIR, atomic force microscopy, electron diffraction, and high resolution transmission electron microscopy (HRTEM) showing the formation of single phase CZTS nanoparticles with sizes between 5-20 nm and tetragonal Kesterite structure type. The electronic properties were studied by X-ray photoelectron spectroscopy (XPS) and ultraviolet photoelectron spectroscopy (UPS), confirming the presence of Cu (I), Zn(II), Sn(IV), and providing an estimated band gap of 1.4 eV. Moreover, Scanning electron mic...
    ABSTRACT This paper present a new method for growing thin films of CZTS using a solution-based chemical route; this consist in sequential deposition of Cu2-xS, SnS and ZnS films by CBD followed by annealing in nitrogen atmosphere. The... more
    ABSTRACT This paper present a new method for growing thin films of CZTS using a solution-based chemical route; this consist in sequential deposition of Cu2-xS, SnS and ZnS films by CBD followed by annealing in nitrogen atmosphere. The Cu2-xS and SnS precursors were deposited by diffusion membranes assisted CBD technique and the ZnS by conventional CBD method. Diffusion membranes are used to optimize the kinetic growth through a moderate control of the release of the metal into the solution by osmosis. The optical, structural and morphological al properties of the CBD depositedCZTS films were compared with those of a reference CZTS film grown by sequential deposition of CuS, SnS and ZnS films, deposited by co-evaporation of the elemental precursors. X-ray diffraction (XRD) and Raman spectroscopy were used to characterize the structural properties of both type of CZTS films. Optical and morphological properties were also studied by spectral transmittance, AFM (atomic force microscopy) and SEM (scanning electron microscopy) measurements. Structural characterization revealed that the evaporated samples present only the Cu2ZnSnS4 phase grown with tetragonal kesterite type structure, whereas the CBD deposited samples present a mixture of the Cu2ZnSnS4 and Cu2-xS phases.
    The differentiation of intestinal intraepithelial lymphocytes (IEL) remains controversial, which may be due in part to the phenotypic complexity of these T cells. We have investigated here the development of IEL in mice on the... more
    The differentiation of intestinal intraepithelial lymphocytes (IEL) remains controversial, which may be due in part to the phenotypic complexity of these T cells. We have investigated here the development of IEL in mice on the recombination activating gene (RAG)-2−/− background which express a T cell antigen receptor (TCR) transgene specific for an H-Y peptide presented by Db (H-Y/Db × RAG-2− mice). In contrast to the thymus, the small intestine in female H-Y/Db × RAG-2− mice is severely deficient in the number of IEL; TCR transgene+ CD8αα and CD8αβ are virtually absent. This is similar to the number and phenotype of IEL in transgenic mice that do not express the Db class I molecule, and which therefore fail positive selection. Paradoxically, in male mice, the small intestine contains large numbers of TCR+ IEL that express high levels of CD8αα homodimers. The IEL isolated from male mice are functional, as they respond upon TCR cross-linking, although they are not autoreactive to sti...
    Pituitary human PRL (hPRL) antiserum inhibits growth of B-lymphoblastoid cells in vitro, but the mechanism of inhibition is unclear. In this study the mechanism of inhibition of human B-cell growth by anti-hPRL was explored with an... more
    Pituitary human PRL (hPRL) antiserum inhibits growth of B-lymphoblastoid cells in vitro, but the mechanism of inhibition is unclear. In this study the mechanism of inhibition of human B-cell growth by anti-hPRL was explored with an Epstein-Barr virus nuclear antigen (EBNA)-negative Burkitt lymphoma cell line (sfRamos) that proliferates continuously in serum-free medium with human transferrin as the only protein supplement. The data show that antiserum immunoglobulin fraction G (IgG) to pituitary hPRL, but not nonimmune serum IgG, completely inhibited the growth of sfRamos cells. In addition, anti-hPRL IgG identified a single band (29 kDa) in sfRamos spent medium, but not in fresh serum-free medium or in human transferrin, as demonstrated by sodium dodecyl sulfate-reducing polyacrylamide gel electrophoresis and Western immunoblot analysis. Polyacrylamide gel electrophoresis and Western analysis of a mixture containing sfRamos spent medium and excess pituitary hPRL established that the sfRamos 29-kDa PRL-like protein (PLP29) and pituitary hPRL (23 kDa) were electrophoretically distinct. Finally, sfRamos spent medium, but not fresh serum-free medium, was mitogenic for sfRamos and Nb2, a PRL-sensitive node rat lymphoma cell line. These findings demonstrate that PLP29 is biologically and immunologically like pituitary hPRL, but is electrophoretically different from this hormone. We suggest that PLP29 is secreted as an autocrine growth factor by sfRamos Burkitt lymphoma cells during continuous serum-free growth.
    Oxidized 1-palmitoyl-2-arachidonoyl- sn -glycero-3-phosphorylcholine (OxPAPC), which has been shown to accumulate in atherosclerotic lesions and other sites of chronic inflammation, activates endothelial cells (EC) to bind monocytes by... more
    Oxidized 1-palmitoyl-2-arachidonoyl- sn -glycero-3-phosphorylcholine (OxPAPC), which has been shown to accumulate in atherosclerotic lesions and other sites of chronic inflammation, activates endothelial cells (EC) to bind monocytes by activation of endothelial β1 integrin and subsequent deposition of fibronectin on the apical surface. Our previous studies suggest this function of OxPAPC is mediated via a Gs protein–coupled receptor (GPCR). PEIPC (1-palmitoyl-2-epoxyisoprostane E2- sn -glycero-3-phosphorylcholine) is the most active lipid in OxPAPC that activates this pathway. We screened a number of candidate GPCRs for their interaction with OxPAPC and PEIPC, using a reporter gene assay; we identified prostaglandin E2 receptor EP2 and prostaglandin D2 receptor DP as responsive to OxPAPC. We focused on EP2, which is expressed in ECs, monocytes, and macrophages. OxPAPC component PEIPC, but not POVPC, activated EP2 with an EC 50 of 108.6 nmol/L. OxPAPC and PEIPC were also able to comp...
    Subacute stent thrombosis occurred in a patient 34 days after receiving a heparin‐coated (HC) stent. The patient developed heparin‐induced thrombocytopenia and diffuse thrombosis after the stent was placed. This raises the concern that... more
    Subacute stent thrombosis occurred in a patient 34 days after receiving a heparin‐coated (HC) stent. The patient developed heparin‐induced thrombocytopenia and diffuse thrombosis after the stent was placed. This raises the concern that patients who develop heparin‐associated antibodies in the context of a recently placed HC stent may have an increased risk for subacute stent thrombosis. Cathet Cardiovasc Intervent 2003;58:80–83. © 2003 Wiley‐Liss, Inc.
    Pulmonary complications of hematopoietic stem cell transplantation (HSCT) are responsible for significant morbidity and mortality. There is no literature to date on the use of extracorporeal membranous oxygenation (ECMO) in patients with... more
    Pulmonary complications of hematopoietic stem cell transplantation (HSCT) are responsible for significant morbidity and mortality. There is no literature to date on the use of extracorporeal membranous oxygenation (ECMO) in patients with pulmonary manifestations of chronic graft-versus-host disease after HSCT. We describe the successful use of ECMO for refractory respiratory failure in such a patient.