David Gela
University of South Bohemia, Facluty of Fisheries and Protection of Water, Department Member
Essential fatty acids (FAs) in food have a positive effect on human health. Hence, high attention has been devoted to improvement of FAs content in flesh of farmed animals through nutrition. Information about genetic variation and... more
Essential fatty acids (FAs) in food have a positive effect on human health. Hence, high attention has been devoted to improvement of FAs content in flesh of farmed animals through nutrition. Information about genetic variation and improvement of FAs are rather scarce. During an experimental selective breeding program in common carp conducted under semi-intensive pond management, a genetic variation of FAs content in fillet of market size common carps (1.4 kg) was investigated. For this purpose, 158 individuals from a full factorial mating of 7 females and 36 males were selected. Content of FAs in flesh was analysed by gas-liquid chromatography. Multi-trait animal model calculation verified moderate heritability for growth (0.57 ± 0.12) in common carp. Heritability of selected FAs ranged from 0.0 to 0.34, but the values were not significantly different from zero in most cases. Heritability for monounsaturated FAs (MUFA) was 0.14, and was 0.11 for polyunsaturated FAs (PUFA).. Low heri...
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This study tested KUROKURA solution (Kurokura et al., 1984, Aquaculture 37, 267–274) and its modifications (by increasing NaCl content to 160, 180 and 200 mM) on immobilizing properties for sampling and short-term preservation of... more
This study tested KUROKURA solution (Kurokura et al., 1984, Aquaculture 37, 267–274) and its modifications (by increasing NaCl content to 160, 180 and 200 mM) on immobilizing properties for sampling and short-term preservation of potential motility of tench spermatozoa. The immobilizing solution is used because, when collected, the sperm of most samples is contaminated by urine, causing spermatozoa to be
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Heritability estimates with a microsatellite parentage assignment based pedigree are of special interest in common carp cultured under traditional pond conditions. This method reduces common environment effects as all families can be... more
Heritability estimates with a microsatellite parentage assignment based pedigree are of special interest in common carp cultured under traditional pond conditions. This method reduces common environment effects as all families can be grown immediately after hatching mixed in the same pond. We applied this method to study genetics of growth and processing traits in common carp at market size (1.5 kg
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A three-year test of growth performance andoffspring survival from top-crossing of commoncarp hybrids in low altitude (350 m above thesea level, middle European climate) and in highaltitude (750 m above the sea level, middleEuropean... more
A three-year test of growth performance andoffspring survival from top-crossing of commoncarp hybrids in low altitude (350 m above thesea level, middle European climate) and in highaltitude (750 m above the sea level, middleEuropean climate) was terminated ...
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Changes in ionic composition as Na+, K+, Ca 2+ and Mg 2+, osmolality inseminal fluid, percentage of motile spermatozoaand velocity were investigated in response toCPP and different dosage of LHRHa. The lowestvelocity of sperm was observed... more
Changes in ionic composition as Na+, K+, Ca 2+ and Mg 2+, osmolality inseminal fluid, percentage of motile spermatozoaand velocity were investigated in response toCPP and different dosage of LHRHa. The lowestvelocity of sperm was observed after use ...
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Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used two-dimensional polyacrylamide gel electrophoresis and matrix-associated laser... more
Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used two-dimensional polyacrylamide gel electrophoresis and matrix-associated laser desorption/ionization time-of-flight mass spectrometry to explore whether the protein profile of common carp (Cyprinus carpio) spermatozoa is affected by cryopreservation. Fourteen protein spots were significantly altered following cryopreservation. Eleven of these were identified: three as specific membrane proteins (N-ethylmaleimide-sensitive fusion protein attachment protein alpha, cofilin 2, and annexin A4) involved in membrane trafficking, organization, and cell movement; six as cytoplasmic enzymes (S-Adenosylhomocysteine hydrolase, Si:dkey-180p18.9 protein, lactate dehydrogenase B, phosphoglycerate kinase 1, transaldolase 1, and esterase D/formylglutathione hydrolase) involved in cell metabolism, oxidoreductase activity, and signal transduction; and two as transferrin variant C and F. Based on these findings, the authors hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process. Cryopreservation caused changes in spermatozoa protein profiles that may lead to decreased spermatozoa velocity, motility, and fertilization success, and to subsequent ova hatching rate.
Research Interests: Proteomics, Membrane Trafficking, Cryopreservation, Time-of-flight mass spectrometry, Signal Transduction, and 19 moreTheriogenology, Biological Sciences, Animals, Male, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Enzyme, Sperm storage, Fertilization, Oxidative Damage, Carps, Two-Dimensional Gel Electrophoresis, Lactate dehydrogenase, Spermatozoa, Sperm Motility, Membrane Protein, Polyacrylamide Gel Electrophoresis, Cyprinus Carpio, Sperm Quality, and Seminal Plasma
Research Interests: Fish Biology, Fish Reproduction, Aquaculture, Fish Physiology, Theriogenology, and 16 moreBiological Sciences, Cell Differentiation, Animals, Male, Sucrose, Wave propagation, Distilled Water, Fish Sperm Motility, Semen, Spermatozoa, Sperm Motility, SPERM MORPHOLOGY, Sodium Chloride, Seminal Plasma, Full Length Movies, and Mannitol
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The aim of this study was to verify whetherall-female carp populations grow faster thanbisexual ones. All-female mirror and scalypopulations were obtained by crossingmasculinizated females (neomales) of Northernmirror carp (M72) with... more
The aim of this study was to verify whetherall-female carp populations grow faster thanbisexual ones. All-female mirror and scalypopulations were obtained by crossingmasculinizated females (neomales) of Northernmirror carp (M72) with regular females of M72and scaly Ropsa carp (ROP) purebreds,respectively. All-female and bisexual progenyof both mirror and scaly carps were rearedseparately for one year. However, controlgroups of a distinct phenotype of scalinesswere put in ponds with tested fish to make anadjustment for fish weight, which could becaused by environmental influence. Nodifferences were found after eight weeks ofrearing between all-female and bisexualpopulations, but, all-female mirror carppopulation (M72) gained 29.7% more weight(P < 0.0001) by the end of the first year thanthe bisexual (68.5 g ± 15.8 against 52.8 g± 13.9; mean ± S.D., respectively).Before beginning the second growing season,each group was marked and stocked together intriplicate ponds. Corrected weig...
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ABSTRACT Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 μms−1) and motility... more
ABSTRACT Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 μms−1) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 ± 0.16 mg ml−1), spermatozoa concentration (0.28 ± 0.27 × 109 spz ml−1), seminal plasma osmolality (51.33 ± 4.91 mOsmol kg−1), the pH (8.49 ± 0.01) and Na+ (18.97 ± 3.65 mm), K+ (2.83 ± 1.36 mm), Ca2+ (0.19 ± 0.06 mm), Mg2+ (0.49 ± 0.23 mm) and Cl− (6.33 ± 0.58 mm) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and iso-electric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon.
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... Sony, SSCDC50AP,) mounted on a dark-field microscope (Olympus BX50, ×200) and illuminated with a stroboscopic lamp (Chadwick-Helmut, 9630 ... decrease of spermatozoa velocity and motility percentage both caused by freeze-thawing... more
... Sony, SSCDC50AP,) mounted on a dark-field microscope (Olympus BX50, ×200) and illuminated with a stroboscopic lamp (Chadwick-Helmut, 9630 ... decrease of spermatozoa velocity and motility percentage both caused by freeze-thawing (Lahnsteiner et al., 2000; Linhart et al ...
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The experiment showed different results after a short (2 min) enzyme alcalase Merck EC 3.4. 21.14 (5.0 ml L− 1 concentration) treatment of tench eggs in contrast to the traditional methods of eliminating egg stickiness involving milk... more
The experiment showed different results after a short (2 min) enzyme alcalase Merck EC 3.4. 21.14 (5.0 ml L− 1 concentration) treatment of tench eggs in contrast to the traditional methods of eliminating egg stickiness involving milk solution (50 g L− 1) treatment for 70 ...
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The aim of the study was to improve the elimination of egg stickiness in tench as an attempt to increase the egg hatching rate. The usual desticking procedure involving treatment with milk solution/clay suspension resulted in a hatching... more
The aim of the study was to improve the elimination of egg stickiness in tench as an attempt to increase the egg hatching rate. The usual desticking procedure involving treatment with milk solution/clay suspension resulted in a hatching rate of 74.1%. The highest hatching ...
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ABSTRACT Summary Siberian sturgeon Acipenser baerii is supposed to by species of functionally tetraploidy (4n). Large-scale screening of ploidy levels of various sturgeon stocks revealed one hexaploid (6n) male specimen producing triploid... more
ABSTRACT Summary Siberian sturgeon Acipenser baerii is supposed to by species of functionally tetraploidy (4n). Large-scale screening of ploidy levels of various sturgeon stocks revealed one hexaploid (6n) male specimen producing triploid spermatozoa. Spermatozoa of three tetraploid individuals were compared with those of the hexaploid specimen in terms of ultrastructure and motility features. All evaluated morphometrical characteristics of the acrosome, the head, the midpiece and the flagellum showed significantly higher values in spermatozoa of 6n in comparison with 4n Siberian sturgeon males. Mean values of velocities at the beginning of activation differed highly significant between spermatozoa of 6n (155.19 and 158.22 μm s−1) and 4n males (181.05 and 179.21 μm s−1) in time 15 and 30 s post activation. Nevertheless their velocities did not show significant differences between spermatozoa of 6n (149.35 and 154.16 μm s−1) and 4n (158.00 and 152.66 μm s−1) in 30 and 45 s post activation. It was supposed that the bigger head of 6n male spermatozoa inhibited motility at the beginning of activation, but later the longer flagellum and bigger midpiece with higher number of mitochondria can partially balance the increased sizes of heads in spermatozoa movement of male with higher ploidy level.
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... on a dark-field microscope (Olympus BX50, 200·, Olympus, Tokyo, Japan) and illumi-nated with a stroboscopic lamp (Chadwick-Helmut, El ... Psenicka, M.; Dietrich, GJ; Wojtczak, M.; Nynca, J.; Rodina, M.; Linhart, O.; Cosson, J.;... more
... on a dark-field microscope (Olympus BX50, 200·, Olympus, Tokyo, Japan) and illumi-nated with a stroboscopic lamp (Chadwick-Helmut, El ... Psenicka, M.; Dietrich, GJ; Wojtczak, M.; Nynca, J.; Rodina, M.; Linhart, O.; Cosson, J.; Ciereszko, A., 2008: Acrosome staining and motility ...
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This study summarizes optimization of techniques for common carp artificial propagation including improvements of activation solution (AS), the process of insemination, and elimination of egg stickiness. The optimum gamete ratio for good... more
This study summarizes optimization of techniques for common carp artificial propagation including improvements of activation solution (AS), the process of insemination, and elimination of egg stickiness. The optimum gamete ratio for good fertilization and hatching rate ranged from ...
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Kocour, M., Gela, D., Rodina, M. and Linhart, O.(2005), Testing of performance in common carp Cyprinus carpio L. under pond husbandry conditions I: top-crossing with Northern mirror carp. Aquaculture Research, 36: 1207–1215. doi:... more
Kocour, M., Gela, D., Rodina, M. and Linhart, O.(2005), Testing of performance in common carp Cyprinus carpio L. under pond husbandry conditions I: top-crossing with Northern mirror carp. Aquaculture Research, 36: 1207–1215. doi: 10.1111/j. 1365-2109.2005. ...