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Article
Mediating role of root-exuded secondary metabolites in intraspecific interactions with Haloxylon ammodendron
Root exudates are pivotal in soil carbon and nutrient cycling, enhancing nutrient availability and plant resource utilization efficiency under stress. Despite challenges in obtaining accurate soil-borne signal...
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Article
Open AccessExpanding the targeting scope of CRISPR/Cas9-mediated genome editing by Cas9 variants in Brassica
CRISPR/Cas9, presently the most widely used genome editing technology, has provided great potential for functional studies and plant breeding. However, the strict requirement for a protospacer adjacent motif (...
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Article
Induction of Peroxiredoxin 1 by Hypoxia Promotes Cellular Autophagy and Cell Proliferation in Oral Leukoplakia via HIF-1α/BNIP3 Pathway
Hypoxia is a key trigger in the transformation of oral leukoplakia into oral cancer. However, it is still too early to determine the role of hypoxia in the development of oral leukoplakia. Prx1, an antioxidant...
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Article
Stay in touch with the endoplasmic reticulum
The endoplasmic reticulum (ER), which is composed of a continuous network of tubules and sheets, forms the most widely distributed membrane system in eukaryotic cells. As a result, it engages a variety of orga...
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Article
Disentangling the Ecological Processes and Driving Forces Shaping the Seasonal Pattern of Halobacteriovorax Communities in a Subtropical Estuary
Halobacteriovorax are predatory bacteria that have a significant ecological role in marine environments. However, understanding of dynamics of populations, driving forces, and community composition of Halobacter...
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Article
Development of an RT-PCR-based RspCas13d system to detect porcine deltacoronavirus
Porcine deltacoronavirus (PDCoV) is an enteropathogen that causes diarrhea in piglets and may undergo cross-species transmission. The prevention and control of PDCoV are complicated, and a sensitive, specific,...
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Article
Serine protease NAL1 exerts pleiotropic functions through degradation of TOPLESS-related corepressor in rice
NARROW LEAF 1 (NAL1) is a breeding-valuable pleiotropic gene that affects multiple agronomic traits in rice, although the molecular mechanism is largely unclear. Here, we report that NAL1 is a serine protease and...
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Article
Open AccessA novel tyrosine tRNA-derived fragment, tRFTyr, induces oncogenesis and lactate accumulation in LSCC by interacting with LDHA
Transfer (t)RNA-derived small RNA (tsRNA), generated from precursor or mature tRNA, is a new type of small non-coding RNA (sncRNA) that has recently been shown to play a vital role in human cancers. However, i...
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Article
Open AccessTOPK promotes the growth of esophageal cancer in vitro and in vivo by enhancing YB1/eEF1A1 signal pathway
T-LAK-originated protein kinase (TOPK), a dual specificity serine/threonine kinase, is up-regulated and related to poor prognosis in many types of cancers. Y-box binding protein 1 (YB1) is a DNA/RNA binding pr...
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Article
The Palmitoylation/Depalmitoylation Cycle is Involved in the Inhibition of AMPA Receptor Trafficking Induced by Aluminum In Vitro
To study the effect of the palmitoylation/depalmitoylation cycle on the inhibition of ɑ-amino-3-hydroxy-5-methyl-4-isoxazolpropionic acid (AMPA) receptor trafficking induced by aluminum (Al) in vitro. Five dif...
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Article
Open AccessEstablishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis
Cotton has tremendous economic value worldwide; however, its allopolyploid nature and time-consuming transformation methods have hampered the development of cotton functional genomics. The protoplast system ha...
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Article
Diet and feeding ecology of Whooper Swan (Cygnus cygnus) and Tundra Swan (C. columbianus) at the Yellow River Wetland of Baotou in Spring Season
Interspecific competition is an important mechanism of ecological community construction, which potentially contributes to resource differentiation and promotes the coexistence of sympatric species. Determinin...
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Article
Open AccessComparative proteomic analysis on chloroplast proteins provides new insights into the effects of low temperature in sugar beet
Low temperature, which is one of the main environmental factors that limits geographical distribution and sucrose yield, is a common abiotic stress during the growth and development of sugar beet. As a regulat...
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Article
Iron metabolism protein transferrin receptor 1 involves in cervical cancer progression by affecting gene expression and alternative splicing in HeLa cells
Transferrin receptor 1 (TfR1), encoded by TFRC, is a key regulator of iron homeostasis and plays important roles in many diseases, including cancers.
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Article
Open AccessPrecision agriculture technology adoption: a qualitative study of small-scale commercial “family farms” located in the North China Plain
Precision agriculture (PA) technologies offer a potential solution to food security and environmental challenges but, will only be successful if adopted by farmers. Adoption in China lags behind that in some d...
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Protocol
A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme
DNAzymes can be obtained by in vitro selection, when specific analytes such as porphyrins are used as targets. In this process, the immobilization of targets serving as a major step is not suitable for the sel...
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Article
The construction of a dual-functional strain that produces both polysaccharides and sulfotransferases
Heparosan is used as the starting polysaccharide sulfated using sulfotransferase to generate fully elaborate heparin, a widely used clinical drug. However, the preparation of heparosan and enzymes was consider...
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Article
Identification of glutathione transferase gene associated with partial resistance to Sclerotinia stem rot of soybean using genome-wide association and linkage mapping
Association and linkage mapping techniques were used to identify and verify single nucleotide polymorphisms (SNPs) associated with Sclerotinia sclerotiorum resistance. A novel resistant gene, GmGST , was cloned a...
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Article
Open AccessTransplantation of human induced pluripotent stem cell-derived neural crest cells for corneal endothelial regeneration
The corneal endothelium maintains corneal hydration through the barrier and pump function, while its dysfunction may cause corneal edema and vision reduction. Considering its development from neural crest cell...
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Article
Downregulation of N-myc Interactor Promotes Cervical Cancer Cells Growth by Activating Stat3 Signaling
N-myc interactor (NMI), a member of the oncogene Myc family, has been reported to be closely related to the development of cancer. However, the character of NMI in cervical carcinoma has not been reported. Her...