Malignant mesothelioma (MM) is a relatively rare cancer that occurs almost exclusively following respiratory exposure to asbestos in humans. Its pathogenesis is closely associated with iron overload and oxidative stress in mesothelial... more
Malignant mesothelioma (MM) is a relatively rare cancer that occurs almost exclusively following respiratory exposure to asbestos in humans. Its pathogenesis is closely associated with iron overload and oxidative stress in mesothelial cells. On fiber exposure, mesothelial cells accumulate fibers simultaneously with iron, which either performs physical scissor function or catalyzes free radical generation, leading to oxidative DNA damage such as strand breaks and base modifications, followed by activation of intracellular signaling pathways. Chrysotile, per se without iron, causes massive hemolysis and further adsorbs hemoglobin. Exposure to indigestible foreign materials also induces chronic inflammation, involving consistent generation of free radicals and subsequent activation of NALP3 inflammasomes in macrophages. All of these contribute to mesothelial carcinogenesis. Genomic alterations most frequently involve homozygous deletion of INK4A/4B, and other pathways such as Hippo and...
In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an... more
In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an experiment was designed with an objective to study the effect of different cytokinins (BAP and Zeatin) on shoot proliferation of two olive cultivars (Earlik and BARI Zaitoon-2). In olive Rugini medium, BAP and Zeatin were added alone and in combination at a concentration of 1 mg L-1 and 2 mg L-1. Experiments were arranged according to completely randomized design (CRD) with three replications per treatment. Results showed that both the olive cultivars performed well when BAP + Zeatin were supplemented to the medium at 2+2 mg L-1 concentration. However, the cultivar “BARI Zaitoon-2” had the highest shooting percentage (90.67 %) and number of nodes per shoot (8.33) in treatment containing BAP (2 mg L-1) + Zeatin (2 mg L-1), whereas, “Earlik” at same concentration had more shoot length (9.10 cm) and number of shoots per explant (3.67). Thus, it was concluded that the findings of these results will help in the future for developing shoot proliferation protocols for other olive cultivars. Moreover, these protocols can also be further be used in other woody plants micro propagation
This paper describes effects of different concentrations of sucrose (20, 30 and 40 g/l), BAP (0.0 and 2 mg/l) as well as MS strength basal media (full, ½ MS and ¼ MS) on the in vitro shoot proliferation in Tissue Culture Laboratory,... more
This paper describes effects of different concentrations of sucrose (20, 30 and 40 g/l), BAP (0.0 and 2 mg/l) as well as MS strength basal media (full, ½ MS and ¼ MS) on the in vitro shoot proliferation in Tissue Culture Laboratory, University of Tabriz. Nodes were cut from in vitro potato shoots and cultured on MS strength basal media for shoot proliferation. All cultures were of 16h light/ 8h dark photoperiod and at 25 ±2 o C in growth chamber. This experiment was carried out factorial experiment based on completely randomized design with four replications. After 4 weeks of culture, number of lateral shoots and nodes, shoot length, root number and callus induction percentage was measured. Analysis of variance showed that numbers of lateral shoots and node, shoots length, root number and callus induction percentage were influenced by studied factors. Number of lateral shoots was increased by using 2 mg/l BAP, but main shoot length was declined by addition of BAP on culture media. The number of nodes in MS full strengths was higher than 1/2MS and ¼ MS media. Maximum root number was observed in the media without BAP and high concentrations of sucrose. Minimum callus induction (an undesirable trait in the proliferation stage) was observed in free BAP media.
Possibility of improving physiological traits and minituber yield of potato cultivars (cvs. Agria and Fontane) was investigated by application of plant growth regulators (BAP, ABA and BAP+ABA) at tuber initiation stage. Regardless of the... more
Possibility of improving physiological traits and minituber yield of potato cultivars (cvs. Agria and Fontane) was investigated by application of plant growth regulators (BAP, ABA and BAP+ABA) at tuber initiation stage. Regardless of the cultivars, Net photosynthesis rate (Np), actual quantum yield (Φ), stomatal conductance (g s) and Transpiration rate (T r) of BAP-treated leaves were superior to those of the control. For Agria, the greatest Chlorophyll content (Chl) was observed in BAP-treated plants, while the highest Chl for Fontane was observed in ABA-treated plants. Increasing Np and Chl content were associated with higher Soluble Carbohydrate content (SC). BAP+ABA application increased SC of leaflets in both cultivars compared with the control. Tuber Yield per Plant (Y/P), Mean Tuber Weight (MTW), and Tuber Number (TN) were stimulated by foliar treatment of plants with PGRs compared with the untreated ones, but there were significant interactions between cultivar and hormone type. Positive correlation between SC and Y/P (r= 0.97*) and MTW (r= 0.97*) were observed in Agria. Leaf area as well as dry and fresh weight of aerial parts of the BAP+ABA-treated plants were more than the untreated plants and other PGR treatments. These results indicate that either of BAP, ABA, or their combination could be effectively used to improve physiological traits and tuber yield of these cultivars, although, Agria responded more prominently to PGRs than Fontane.
BRCA1-associated protein-1 (BAP1) is a 729 residue, nuclear-localized deubiquitinating enzyme (DUB) that displays tumor suppressor properties in the BAP1-null NCI-H226 lung carcinoma cell line. Studies that have altered BAP1 cellular... more
BRCA1-associated protein-1 (BAP1) is a 729 residue, nuclear-localized deubiquitinating enzyme (DUB) that displays tumor suppressor properties in the BAP1-null NCI-H226 lung carcinoma cell line. Studies that have altered BAP1 cellular levels or enzymatic activity have reported defects in cell cycle progression, notably at the G1/S transition. Recently BAP1 was shown to associate with the transcriptional regulator host cell factor 1
Abstract: Aloe vera has valuable medicinal properties and is commercially used inpharmaceutical, cosmetic and food industries. An efficient micro propagation method hasbeen developed in Aloe vera plants using the shoot tip explants... more
Abstract: Aloe vera has valuable medicinal properties and is commercially used inpharmaceutical, cosmetic and food industries. An efficient micro propagation method hasbeen developed in Aloe vera plants using the shoot tip explants cultured on MS ...
In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an... more
In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an experiment was designed with an objective to study the effect of different cytokinins (BAP and Zeatin) on shoot proliferation of two olive cultivars (Earlik and BARI Zaitoon-2). In olive Rugini medium, BAP and Zeatin were added alone and in combination at a concentration of 1 mg L-1 and 2 mg L-1. Experiments were arranged according to completely randomized design (CRD) with three replications per treatment. Results showed that both the olive cultivars performed well when BAP + Zeatin were supplemented to the medium at 2+2 mg L-1 concentration. However, the cultivar “BARI Zaitoon-2” had the highest shooting percentage (90.67 %) and number of nodes per shoot (8.33) in treatment containing BAP (2 mg L-1) + Zeatin (2 mg L-1), whereas, “Earlik” at same ...
Phalaenopsis, one of plant genus in Orchidaceae, is commercially produced as pot plant and cut flower because it has high economical value in world market, including Indonesia. Several micropropagation methods have been developed for... more
Phalaenopsis, one of plant genus in Orchidaceae, is commercially produced as pot plant and cut flower because it has high economical value in world market, including Indonesia. Several micropropagation methods have been developed for Phalaenopsis, but not all of these methods can be used for commercial micropropagation because of differences in survival rate, PLB formation and plantlet regeneration. Therefore, continued studies about the most important stages in Phalaenopsis micropropagation, which are multiplication and germination stages, are needed. Combination of NAA and BAP produce PLB formation and multiplication. Objectives of this study were to determine the effect of NAA and BAP concentrations in PLB multiplication, and germination of PLB from this multiplication results grown in medium without plant growth regulators. Effects of NAA and BAP combinations, which are 0.1 ppm NAA and 1 ppm BAP; 0.1 ppm NAA and 5 ppm BAP; 0.1 ppm NAA and 10 ppm BAP; 0.1 ppm NAA and 20 ppm BAP; ...
