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6 Endomembrane System

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Endomembrane

System
Endomembrane system
System of sacs and membranes in the cell

 Rough Endoplasmic Reticulum


 Smooth Endoplasmic Reticulum

 Golgi Apparatus

 Lysosomes

 Peroxisomes

 Vacuoles
Endoplasmic reticulum
 Endoplasmic reticulum (ER) is
only found in eukaryotes 

 It is the transport network for


molecules targeted for
modification or a specific
destination
Images of cells with ER stained green.
Nucleus is blue and mitochondria are red
 ER has two forms:
 rough endoplasmic reticulum
(RER)
 smooth endoplasmic reticulum
(SER)
Endoplasmic reticulum
 Rough endoplasmic reticulum (RER)
 Has attached ribosomes
 Site of assembly of proteins for transport
 Assembled proteins may be modified

 Smooth endoplasmic reticulum (SER)


 Synthesizes lipids such as phospholipids and
steroids
 Helps to detoxify wastes, drugs and alcohol
 Involved in carbohydrate metabolism
 Involved in regulation of calcium levels
RER and Protein Synthesis
•Ribosomes bound to the RER
at any one time are not a stable
part of the organelle's structure

•Ribosomes are constantly


being bound and released from
the membrane.

•A ribosome only binds to the


ER once it begins to synthesize
a protein
Golgi Apparatus
 Primary function - process and package proteins and
lipids that are synthesized elsewhere

 Very important in processing proteins for secretion

 Vesicles that enter the Golgi Apparatus are processed


and then pinch off on the opposite side to form a new
vesicle

 Also produces lysosomes: protein containing vesicles


within cells that digest substances
Lysosomes and Peroxisomes
 Lysosomes
 Contain digestive enzymes (acid hydrolases)
 Digest excess or worn-out organelles, food particles, and
engulfed viruses or bacteria
 Made by Golgi

 Peroxisomes
 Contain enzymes that rid the cell of toxic peroxides
 Made from other peroxisomes (not from Golgi)

 The cell could not house these destructive


enzymes if they were not contained in a
membrane-bound system

 These organelles are often called a "suicide bag"


because of their ability to destroy the cell
Exocytosis and Endocytosis

Getting in and out of cells without passing


through the membrane
Exocytosis
Endocytosis
 Endocytosis: Things that are too large to
pass through the membrane enter the cell
in vesicles

 3 main types of transport


 Phagocytosis: large objects e.g., bacteria
 Pinocytosis: dissolved objects
 Receptor-mediated Endocytosis: vesicles
formed in response to a particular chemical
e.g., insulin
Vacuoles
 Store food and waste or extra
water

 Some cells, e.g., Amoeba, have


contractile vacuoles, which are
able to pump water out of the
cell if there is too much water

 Vacuoles of plant cells are


usually larger than those of
animals
 A contractile vacuole is surrounded by radial canals which absorb
water from the cytoplasm and move it into the vacuole. Once filled
with water, the contractile vacuole contracts and forces the water out
of the cell. https://www.youtube.com/watch?v=MxbwiACd0Tw

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