The blood profile, nutritional-metabolic status, and growth performance of cattle receiving probiotic lactobacilli for 105 days from entry to a feedlot system were evaluated. The trial involved 60 Brangus/Braford castrated steers. They... more
The blood profile, nutritional-metabolic status, and growth performance of cattle receiving probiotic lactobacilli for 105 days from entry to a feedlot system were evaluated. The trial involved 60 Brangus/Braford castrated steers. They were randomly allocated in pens (n = 15/treatment) and in-feed supplemented with different probiotic suspensions (107–108 CFU/mL/day). The suspensions consisted of (A) Lactobacillus acidophilus CRL2074, (B) Limosilactobacillus fermentum CRL2085, or (C) multistrain CRL2074 + CRL2085 + Limosilactobacillus mucosae CRL2069 formulations, against the control group. Individual samples were taken from each animal thrice throughout the assay (0, 45, and 105 days). Analysis of clinical parameters showed optimal animal body condition and sensorium state. Isolated nasal discharges and the absence of diarrhea were observed at 105 days in the group administered with multistrain and Lim. fermentum CRL2085 probiotics. An increasing trend was observed in cattle growth throughout the trial, which was calculated based on the height at the withers and the thoracic diameter. Weight increase and daily weight gain (1.31 ± 0.12 kg and 1.21 ± 0.21 kg/day, respectively) were maximum for cattle supplied with CRL2085 and multistrain probiotics. Blood and serum parameters were within referential ranges for the control and probiotics-supplemented groups; however, mean values of hematocrit were higher, while the serum glucose, lipid profile, and C-reactive protein values were lower in all the groups. In addition, the multistrain probiotic formulation exhibited higher numbers of cultivable lactic acid bacteria and a slight decrease in the number of enterobacteria in feces at the end of the trial. Therefore, there is great potential for multistrain probiotic formulation to improve the overall performance of feedlot beef cattle.
Research Interests: Microbiology, Probiotics, Biology, Argentina, Medicine, and 15 moreMolecular typing, Humans, Bacteriocins, Female, Lactobacillus, Candida albicans, Antimicrobial, Hydrogen Peroxide, European, Probiotic, Species Specificity, Lactic Acid, Antibiosis, Paediatrics and reproductive medicine, and Biological products
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Diarrhea is one of the most frequent diseases affecting newborn calves in intensive systems. Several strategies were proposed to protect and improve health, as probiotics. This work was directed to design a product containing freeze dried... more
Diarrhea is one of the most frequent diseases affecting newborn calves in intensive systems. Several strategies were proposed to protect and improve health, as probiotics. This work was directed to design a product containing freeze dried bacteria, vitamins and minerals. Also, to optimize conditions with lyoprotectors, to combine strains and add vitamins, minerals and inulin to the product. The lyoprotectors were: milk, milk-whey and lactose and products were stored for six month at 4°C. Combined bacteria were freeze-dried in milk and the final products added with minerals, vitamins and inulin. The viable cells were determined by the plate count assay and antibiotic profiles to differentiate strains. Lactobacillus johnsonii CRL1693, L. murinus CRL1695, L. mucosae CRL1696, L. salivarius CRL1702, L. amylovorus CRL1697 and Enterococcus faecium CRL1703 were evaluated. The optimal conditions were different for each strain. Milk and milk-whey maintained the viability during the process and storage after six month for most of the strains, except for L.johnsonii. Lactose did not improve cell's recovery. L. murinus was viable for six months in all the conditions, with similar results in enterococci. In strains combined before freeze-dried, the viability decreased deeply, showing that one-step process with bacteria mixtures, vitamins and minerals was not adequate. Freeze-dried resistance depends on each strain, and must be lyophilized individually.
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Lactobacilli are the predominant microorganisms of the healthy human vagina. A novel alternative for the prevention and treatment of female urogenital tract infections (UGTI) is the inclusion of these microorganisms as active... more
Lactobacilli are the predominant microorganisms of the healthy human vagina. A novel alternative for the prevention and treatment of female urogenital tract infections (UGTI) is the inclusion of these microorganisms as active pharmaceutical ingredients in probiotic formulas, and more recently in female hygienic products. Probiotics are defined as “live microorganisms that, when administered in adequate amounts, confer a health benefit on the host.” A list of requirements must be considered during the development of probiotic product/formula for the female urogenital tract (UGT). This review aims to resume the requirements, probiotic characteristics, and clinical trial applied to determine the effect of probiotic and potentially probiotic strains on different woman’s physiological and pathological conditions, and in preterm birth prevention. A revision of female hygienic products available in the world market is included, together with novel studies applying nanotechnology for Lactobacillus incorporation in hygienic products. Further studies and well‐designed clinical trials are urgently required to complement the current knowledge and applications of probiotics in the female UGT. The use of probiotic formulas and products will improve and restore the ecological equilibrium of the UGT microbiome to prevent and treat UGTI in women under different conditions.
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The aim of this work was to formulate a culture medium of lower cost than conventional laboratory media, in order to simultaneously obtain high amounts of both biomass and bacteriocin of vaginal Lactobacillus salivarius CRL 1328. The... more
The aim of this work was to formulate a culture medium of lower cost than conventional laboratory media, in order to simultaneously obtain high amounts of both biomass and bacteriocin of vaginal Lactobacillus salivarius CRL 1328. The growth assays under different culture conditions were performed by using a 2(8-2) central composite experimental design, with a central point and sixteen additional points. The factors taken into consideration were glucose, lactose, yeast extract, tryptone, ammonium citrate, sodium acetate, MgSO4 and MnSO4. The simultaneous presence of a carbon source (mainly glucose), a nitrogen source (mainly yeast extract) and salts (mainly MnSO4, MgSO4 and sodium acetate) allowed the highest cell biomass and bacteriocin levels to be reached in the experimental design. Through the application of the desirability function, several optimal medium compositions to achieve efficient production of biomass and bacteriocin were predicted. The optimized growth media allow a cost reduction of around 25 to 40% compared with conventional broths. The results obtained represent an advance in the search of the most suitable strategies for the production of bioactive compounds for pharmaceutical products to prevent or treat female urogenital infections.
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Research Interests: Microbiology, Food Science, Probiotics, Biology, Experimental Design, and 15 moreMedicine, Humans, Female, Lactobacillus, Catalase, Hydrogen Peroxide, Probiotic, Agar, Optimal Growth, Culture Media, Optimality Condition, Mixed Culture, Paediatrics and reproductive medicine, Lactobacillus paracasei, and Lactobacillus crispatus
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Lactic acid bacteria (LAB) were isolated, identified, and characterized from pig feces at various growth stages and feed rations in order to be used as probiotic feed additives. Lactic acid bacteria numbers ranged from 7.10 ± 1.50 to 9.40... more
Lactic acid bacteria (LAB) were isolated, identified, and characterized from pig feces at various growth stages and feed rations in order to be used as probiotic feed additives. Lactic acid bacteria numbers ranged from 7.10 ± 1.50 to 9.40 log CFUs/g for growing and lactating pigs, respectively. Isolates (n = 230) were identified by (GTG)5-polymerase chain reaction and partial sequence analysis of 16S rRNA. Major LAB populations were Limosilactobacillus reuteri (49.2%), Pediococcus pentosaceus (20%), Lactobacillus amylovorus (11.4%), and L. johnsonii (8.7%). In-vitro assays were performed, including surface characterization and tolerance to acid and bile salts. Several lactobacilli exhibited hydrophobic and aggregative characteristics and were able to withstand gastrointestinal tract conditions. In addition, lactobacilli showed starch- and phytate-degrading ability, as well as antagonistic activity against Gram-negative pathogens and the production of bacteriocin-like inhibitory substances. When resistance or susceptibility to antibiotics was evaluated, high phenotypic resistance to ampicillin, gentamicin, kanamycin, streptomycin, and tetracycline and susceptibility towards clindamycin and chloramphenicol was observed in the assayed LAB. Genotypic characterization showed that 5 out of 15 resistance genes were identified in lactobacilli; their presence did not correlate with phenotypic traits. Genes erm(B), strA, strB, and aadE conferring resistance to erythromycin and streptomycin were reported among all lactobacilli, whereas tet(M) gene was harbored by L. reuteri and L. amylovorus strains. Based on these results, 6 probiotic LAB strains (L. reuteri F207R/G9R/B66R, L. amylovorus G636T/S244T, and L. johnsonii S92R) can be selected to explore their potential as direct feed additives to promote swine health and replace antibiotics.
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Vaginal lactobacilli (LAB) in probiotic formulas constitute a promising alternative for microbiome reconstitution and for the prevention and treatment of urogenital infections. A double-blind, randomised clinical trial was conducted to... more
Vaginal lactobacilli (LAB) in probiotic formulas constitute a promising alternative for microbiome reconstitution and for the prevention and treatment of urogenital infections. A double-blind, randomised clinical trial was conducted to assess the safety of LAB-gelatine capsules vaginally administered to healthy sexually active women. Participants were randomised into three groups: intervention A: Lactobacillus reuteri CRL1324, Lactobacillus gasseri CRL1263 and CRL1307; intervention B: Lactobacillus rhamnosus CRL1332, L. gasseri CRL1256 and CRL1320; and intervention C: placebo. In a survey and clinical evaluation, participants received a blister with 7 capsules to be administered 1 per day. A second sampling and a new survey were conducted 3-10 days after completing application. Colposcopy was performed to assess adverse effects on vaginal-cervical mucosa. Vaginal swabs were taken for Gram staining to determine the Nugent score, and obtainment of viable-cell cultures to quantify cultivable lactic acid bacteria and pathogens. The main outcomes evaluated were overall satisfaction and secondary effects, including discomfort, urogenital infection, inflammatory response or other symptoms. No significant differences were found in Nugent score or in leukocyte numbers in vaginal samples either before or after the three interventions. However, a tendency to decrease in both the Nugent score and in leukocyte numbers was observed after interventions A and B, though not after C. A significant increase in cultivable lactobacilli was determined after LAB interventions. No severe adverse events were detected. LAB-containing capsules were well tolerated by subjects, so they could be proposed as an adequate alternative to restore vaginal lactobacilli in sexually active women.
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To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model. The major virulence factors of four... more
To assess the preventive effect of different intravaginal (i.va.) doses of Lactobacillus reuteri CRL1324 against vaginal colonization by Group B Streptococcus (GBS) in a murine experimental model. The major virulence factors of four vaginal GBS clinical isolates were determined to select the most virulent strain and set up a murine model of streptococcal vaginal colonization. Later, the effect of four and seven doses of 10(8) viable cells of Lact. reuteri CRL1324 i.va. administered, prior to the GBS challenge was studied. Seven doses of lactobacilli were able to significantly reduce the number of viable GBS cells, while four doses showed no preventive effect. Both doses reduced the leucocyte influx induced by GBS. Seven doses caused a slight increase in the Lact. reuteri CRL1324 vaginal colonization compared with four doses and reduced murine vaginal pH compared to control mice. Lactobacillus reuteri CRL1324 evidenced a preventive effect on GBS vaginal colonization in an experimental mouse model. Maternal GBS colonization is one of the most important risk factors for developing disease in newborns. Lactobacillus reuteri CRL1324 could be considered as a new biological agent to reduce infections caused by this micro-organism.
Research Interests: Microbiology, Probiotics, Biology, Applied microbiology, Medicine, and 15 moreMultidisciplinary, Colonization, Mice, Female, Animals, Lactobacillus, Ciencias Biológicas, Streptococcus, Lactobacilli, Microbiota, Streptococcus agalactiae, Group B Streptococcus, Lactobacillus reuteri, Streptococcal infections, and Urogenital Tract
Research Interests: Microbiology, Probiotics, Medical Microbiology, Biology, Medicine, and 15 moreBiological Sciences, Humans, Mice, Female, Animals, Lactobacillus, Candida albicans, Antimicrobial, Ciencias Biológicas, In Vivo, Microbial Interactions, Lactobacilli, Carboxylic Acids, Bacterial Adhesion, and Medical and Health Sciences
Research Interests: Microbiology, Biofilms, Biology, Applied microbiology, Medicine, and 13 moreMultidisciplinary, Adhesion, Humans, Biofilm, Female, Lactobacillus, Phenotype, Ciencias Biológicas, Vagina, Enseñanza - Aprendizaje Ciencias Naturales Y Exactas, Bacterial Adhesion, Lactobacillus Rhamnosus, and Lactobacillus reuteri
To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains. Fifteen Lactobacillus... more
To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains. Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween. Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay. The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon.
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Research Interests: Chemistry, Food Science, Probiotics, Biology, Medicine, and 15 moreBacteriocins, Lactic Acid Bacteria, Lactobacillus, Gum Arabic, Antimicrobial, Bacteriocin, Ascorbic Acid, Probiotic, Freeze Drying, Lactose, Lactobacillus Salivarius, Drug Stability, Inulin, drug storage, and Pharmacology and pharmaceutical sciences
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This study was perfomed to evaluate the effect of the treatment of norfloxacin, as an antimicrobial agent, on the uropathogenic Escherichia coli colonization in mice, complemented by Lactobacilli as probiotics. Norfloxacin (5.5 mg. Kg-1... more
This study was perfomed to evaluate the effect of the treatment of norfloxacin, as an antimicrobial agent, on the uropathogenic Escherichia coli colonization in mice, complemented by Lactobacilli as probiotics. Norfloxacin (5.5 mg. Kg-1 dose) was administred orally before and after the challenge with E. coli. The animals were also inoculated intra-urethrally with agarose beads containing L. fermentum. The population of the microorganisms in the urogenital tract was measured various days throughout the study. Using either 1 or 3 doses of norfloxacion befor or after the animal had been challenged with E. coli bacteria, reduced the E. coli population the urinary tract. The Norfloxacin treatment after the animals were administered 1 or 3 doses of agarose beads of lactobacilli significantly affected the viability of the lactic acid bacteria in all of the urinary tract organs and the number of E. coly (regardless to when the antibiotic was inoculated) was lower in the mice treated with lactobacilli than in the non-lactobacilli treated mice. Eventhough the survival of the lactobacilli was highly affected by the norfloxacin treatment, the uropathogenic E. coli colonization always decreased more than the lactobacilli population did and often completely disappeared in those animals which were treated with both norfloxacin and probiotic lactobacilli. Through this experimental model one can conclude that lactobacilli increases the effectiveness of the norfloxacin medication in the prevention and treatment of UTI produced by E. coli. Further studies are being performed to determine the mechanisms involved in this probiotic effect.Fil: Silva, Clara del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentin
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Aryloxoalcanoic compounds induce resistance to antibiotic therapy in urinary tract infection caused by Escherichia coli
El presente trabajo se realizó para estudiar las características nutricinales del grano de mijo y de diversos productos obtenidos a partir de grano cocido, de harina y de jugo de mijo, y el impacto que causan el ser ofrecidos como una... more
El presente trabajo se realizó para estudiar las características nutricinales del grano de mijo y de diversos productos obtenidos a partir de grano cocido, de harina y de jugo de mijo, y el impacto que causan el ser ofrecidos como una variante en la dita. Se elaboraron de manera artesanal tres poductos diferentes (jugo, hamburguesa y mamul) y se determinaron las características organolépticas. Se realizó el análisis químico, lo que evidenció que este cereal contiene mayor cantidad de hidratos de carbono y menor de proteínas que otros similares. Se valoraron nutricionalmente los productos elaborados y se realizó la evaluación microbiológica del jugo pasteurizado, demostrando que el número de microorganismos presentes es bajo y está dentro de los estándares microbiológicos, por lo cual es apto para consumo. Asimismo, se inoculó en este sustrato Lactobacillus casei, el que es capaz de crecer y disminuir el pH, sin modificar sus características organolépticas. Se determinó también la satisfacción y la aceptabilidad de los productos en 30 consumidoras vegetarianas y 30 no vegetarianas, las que expresaron que resultan gustosos y aceptables para consumo en una amplia mayoría, no existiendo diferencias de opinión entre ambos grupos.Fil: López, Silvia. Universidad del Norte Santo Tomás de Aquino; ArgentinaFil: Nuñez, Martha Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad del Norte Santo Tomás de Aquino; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentin
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El girasol, cuyo nombre botánico es Helianthus annuusL., es una planta anual familia de las margaritas y sunombre se debe al hecho de que su inflorescencia giramirando hacia el sol. La semilla de girasol es una de lasproducciones más... more
El girasol, cuyo nombre botánico es Helianthus annuusL., es una planta anual familia de las margaritas y sunombre se debe al hecho de que su inflorescencia giramirando hacia el sol. La semilla de girasol es una de lasproducciones más importantes a nivel mundial, siendola Argentina el único país de producción relevante en elhemisferio sur. El girasol en nuestro país se cultivasobre todo en las zonas del NEA, el Sudeste y Sudoestede Buenos Aires y Sur de La Pampa. Su producción estáorientada principalmente a la elaboración de aceite y ensegundo lugar para harina proteica. En los últimos añosla producción de nuestro país descendió un 20%, apesar de lo cual se ubica en el cuarto puesto a nivelmundial, siendo Ucrania, Rusia y la Unión Europea losprincipales productores.La semilla de girasol resulta una opción adecuadapara elaborar una bebida altamente proteica a finde promover un efecto saludable en personas con unpeso por debajo del criterio de normalidad, ya que setrata de un alimento sano y natural, que aporta grasaslibres de colesterol, una buena cantidad de proteínas yun contenido algo menor en hidratos de carbono(Pérez, 2017). Por otra parte, en nuestro país se disponede materias primas adecuadas para complementar labebida que puedan consumir personas de bajo peso oque requieren una alimentación específica.El objetivo de este trabajo fue elaborar una bebida instantáneade valor calórico alto con semilla de girasol,para brindar una alternativa durante el desayuno omerienda en personas con bajo peso. Se elaboró estabebida con harina de semilla de girasol, de avena y dearroz y leche en polvo. Luego se calculó el valor calóricoy la densidad calórica (DC). También se determinó elporcentaje de suministro dietario recomendado (RDA)que cubre la bebida para una dieta de 2.000 kcal.Se conformó un panel de 100 consumidoresno entrenados, de ambos sexos y entre 18-30 años deedad, para realizar una degustación y determinar elgrado de aceptabilidad y satisfacción y evaluar lascaracterísticas organolépticas de la bebida elaborada.También se determinó el nivel de conocimiento sobrelas propiedades benéficas de la semilla de girasol. Labebida elaborada resultó aceptada y satisfactoria para lamayoría de los encuestados.Fil: Tirador, Maria Florencia. Universidad del Norte Santo Tomás de Aquino; ArgentinaFil: Nader, Maria Elena Fatima. Universidad del Norte Santo Tomás de Aquino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentin
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The use of antibiotics to prevent bovine mastitis is responsible for the emergence and selection of resistant strains. Lactic acid bacteria (LAB) could be introduced into animal feed as an alternative prevention method that would bypass... more
The use of antibiotics to prevent bovine mastitis is responsible for the emergence and selection of resistant strains. Lactic acid bacteria (LAB) could be introduced into animal feed as an alternative prevention method that would bypass the risk of resistance development. In previous research, we demonstrated that two probiotic LAB strains isolated from bovine milk were capable of stimulating the production of antibodies and the host’s immune cellular response in the udder. The present study aimed to elucidate whether the antibodies of animals inoculated with these strains were able to increase phagocytosis by neutrophils and inhibit the growth of different mastitis-causing pathogens. Moreover, the effect of LAB on the expression of pro-inflammatory cytokines was assessed. Ten animals were inoculated intramammarily with 106 cells of the two strains at dry-off. The blood serum was tested for its ability to opsonize bovine mastitis pathogens, the in vitro bactericidal activity of bovi...
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The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune... more
The use of lactic acid bacteria (LAB) in animal feed, constitute an alternative tool for bovine mastitis prevention. Previously, two LAB strains were isolated from bovine milk and selected for their probiotics properties. So far, immune response of inoculating LAB in bovine udders at dry-off period has not been investigated. The immunoglobulin isotype levels and memory cell proliferation in blood and milk of animals inoculated with Lactobacillus lactis subsp. lactis CRL1655 and Lactobacillus perolens CRL1724 at dry-off period was studied. Ten animals were inoculated intramammarily with 106 cells of each LAB (IG) and 2 animals used as control (NIG). Milk and blood samples were taken before inoculation and 1, 2, 4, 6, 12 and 24 h and 7 and 14 days after inoculation. Somatic cell count (SCC) in milk, the presence of bovine mastitis pathogens, the levels of antibodies and lymphocyte proliferation were determined. In the IG, the SCC was <250,000 cells/ml up to 4 h after intramammary i...
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Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria... more
Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20-25 °C, respectively. The bacteriocin synthesis was closely growth associated reaching the maximal values at the end of the exponential phase. Since bacteriocins co-production has been evidenced in bacterial genera, a purification of the bacteriocin/s from L. lactis culture supernatants was carried out. The active fraction was purified by cationic-exchange chromatography and then, a RP-HPL...
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The Argentinean medicinal plant Tetraglochin cristatum (Britton) Rothm is used in traditional medicine by inhabitants from Argentinean northwestern highlands (Puna) to treat candidiasis and as anti-inflammatory. To assess the potential of... more
The Argentinean medicinal plant Tetraglochin cristatum (Britton) Rothm is used in traditional medicine by inhabitants from Argentinean northwestern highlands (Puna) to treat candidiasis and as anti-inflammatory. To assess the potential of the crude drug as an anti-Candida agent with anti-inflammatory properties. The bioactivity and phytochemical composition of a dry extract of the plant was investigated. A hydroalcoholic dry extract from T. cristatum was evaluated as antifungal against pathogenic Candida sp. and Saccharomyces cerevisiae isolated from vaginal infections and reference strains, by the microdilution assays. The normal vaginal microbiome in women is characterized by the dominance of lactic acid-producing bacteria, mainly Lactobacillus spp. The effect of T. cristatum extract on Lactobacillus strains was also assayed.The inhibitory effect on proinflammatory enzymes (cyclooxygenase, lipoxygenase and phospholipase A2) and antioxidant capacity was studied. The chemical profil...
Research Interests: Chemistry, Complementary and Alternative Medicine, Biology, Argentina, Ethnopharmacology, and 15 moreMedicine, Antioxidants, Humans, Female, Antioxidant, Candida, Lactobacillus, Antimicrobial, Ciencias Biológicas, Anti Inflammatory, Antifungal Agents, Microbial Sensitivity Tests, Erythrocytes, Hemolysis, and candidiasis vulvovaginal
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This study aims to evaluate potentially beneficial properties of 20 strains of vaginal lactobacilli isolated from women in Tucumán, Argentina, by determining acid and hydrogen peroxide production and auto-aggregation ability. The... more
This study aims to evaluate potentially beneficial properties of 20 strains of vaginal lactobacilli isolated from women in Tucumán, Argentina, by determining acid and hydrogen peroxide production and auto-aggregation ability. The microorganisms were characterised genetically by amplified ribosomal 16S-DNA restriction analysis (ARDRA). Lactobacillus gasseri and L. rhamnosus were the predominant species identified among the 20 vaginal lactobacilli strains. Most achieved low pH values after 12 h incubation at 37 degrees C and produced hydrogen peroxide in static culture. However, pH decrease and semi-quantitative hydrogen peroxide production of most homofermentative lactobacilli were significantly higher than those of heterofermentative lactobacilli. Of the 20 strains studied, only three demonstrated remarkable auto-aggregation patterns. Four strains were selected for possible use in a probiotic product for vaginal application; however, further in vitro study of other potentially probiotic characteristics is required before attempting clinical trials.
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The use of autochthonous microorganisms as probiotics is a novel and promising alternative for the preventive application of antibiotics. Lactic acid bacteria (LAB) are one of the most successfully microorganisms used as probiotics and... more
The use of autochthonous microorganisms as probiotics is a novel and promising alternative for the preventive application of antibiotics. Lactic acid bacteria (LAB) are one of the most successfully microorganisms used as probiotics and applied in different niches. The aquaculture of Piaractus mesopotamicus in Northern Argentina has increased exponentially, but the use of this native species in production systems led to a deficit in the availability of larvae for breeding and fattening. The objective of the present study was to evaluate the effect of the addition of two different formulae containing autochthonous beneficial LAB strains on the survival, mean weight and biomass of larvae under laboratory intensive breeding. Results indicate that the bacterial administration does not cause any significant effect on the variables evaluated. However, statistical analysis showed a tendency of the 6x10 7 CFU L -1 dose of one of the formula to stimulate an increase of mean weight and biomass. On the other hand, the average values of biometrical parameters obtained with the second formula, indicate that the optimum dose is 6x10 4 CFU L -1 . These results and the histological confirmation of the harmfulness of the strains, allowed us to select these doses for their use in a combined probiotic mixture to be tested in future assays.
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Maternal Group B Streptococcus (GBS) colonization is a risk factor for infectious disease in newborns. One promising strategy is the modulation of vaginal defense to increase the host's ability to combat infection. The effect of... more
Maternal Group B Streptococcus (GBS) colonization is a risk factor for infectious disease in newborns. One promising strategy is the modulation of vaginal defense to increase the host's ability to combat infection. The effect of intravaginal (i.va.) Lactobacillus reuteri CRL1324 inoculation on different immune cell populations, cytokines, and immunoglobulin isotypes in a murine model of GBS vaginal colonization was evaluated. Seven i.va. inoculations of L. reuteri CRL1324 previous to GBS challenge showed an immunomodulatory effect on the cells and mediators of innate immunity, decreasing the number of neutrophils induced by the pathogen and increasing the activated macrophage population. Moreover, increases in B lymphocytes and IgA and IgG subclasses were observed in mice inoculated with L. reuteri CRL1324 and then challenged with GBS. Lactobacillus reuteri CRL1324 shows a protective effect against GBS colonization that could be mediated by the modulation of the immune response.
Research Interests: Microbiology, Immunology, Biology, Macrophages, Medicine, and 15 moreColonization, Population, Humans, Mice, Female, Animals, Lactobacillus, Immunomodulation, Clinical Sciences, Neutrophils, Immune system, B Lymphocytes, Lactobacillus reuteri, Paediatrics and reproductive medicine, and Streptococcal infections
The aim of this research was the assessment of antibiotic susceptibility profile on bovine vaginal probiotic Lactobacillus. Antibiotics could affect the colonization of probiotic microorganisms when the host is exposed to therapies for... more
The aim of this research was the assessment of antibiotic susceptibility profile on bovine vaginal probiotic Lactobacillus. Antibiotics could affect the colonization of probiotic microorganisms when the host is exposed to therapies for other infections. We studied the sensibility of four strains of bovine probiotic vaginal Lactobacillus to ampicillin, vancomycin, gentamicin, kanamycin, lincomycin, tetracyclin, chloramphenicol, erythromycin, sulphamethoxazole, metronidazole, nitrofurantoin, aminopenicillin/sulbactam and trimethoprim/sulfamethoxazole. The agar overlay disc diffusion and the dilution methods were performed according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. The Minimal Inhibitory Concentrations (MICs) obtained showed that L. gasseri CRL1412, L. gasseri CRL1421 and L. gasseri CRL1460 have similar susceptibility profiles. L. delbruekii subsp. delbrueckii CRL1461 was the most susceptible strain. The four strains showed high MIC values for ...
Bovine mastitis produces a wide variety of problems in the dairy farm. The treatment of this disease is based on the use of antibiotics which are not always effective. These drugs are also responsible for the presence of residues in the... more
Bovine mastitis produces a wide variety of problems in the dairy farm. The treatment of this disease is based on the use of antibiotics which are not always effective. These drugs are also responsible for the presence of residues in the milk and the increase of antibiotic-resistant strains. Probiotic products were proposed as a valid alternative to antibiotic therapies and are also useful for the prevention of infectious syndromes. With the aim of designing a probiotic product to prevent bovine mastitis, lactic acid bacteria (LAB) were isolated from foremilk samples from different dairy farms in Córdoba-Argentina. One hundred and seventeen LAB were isolated and their beneficial characteristics such as the production of inhibitory substances, surface properties and production of exopolysaccharides (EPS) were assessed. Most of them displayed low degree of hydrophobicity, autoaggregation, EPS negative phenotype and were identified as Enterococcus hirae and Pediococcus pentosaceus. Nine...
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Lactobacilli are the predominant microorganisms in the vaginal tract of human and some homeothermic animals. They can maintain the ecological equilibrium of the tract by protecting against pathogenic microorganisms. In the last few years,... more
Lactobacilli are the predominant microorganisms in the vaginal tract of human and some homeothermic animals. They can maintain the ecological equilibrium of the tract by protecting against pathogenic microorganisms. In the last few years, there has been an increased tendency to use probiotic microorganisms to restore the ecological equilibrium and to protect against infections. This principle has been widely applied to the gastrointestinal tract. More recently, some other studies have reported the application of probiotics in different tracts, for example, the urogenital or respiratory tract. One of the objectives of our group is to design probiotic products for the urogenital tract. With this purpose, lactobacilli were isolated from the human vagina, and later some of them were selected for their probiotic characteristics (production of antagonistic substances or adhesion capability). The application of probiotic products in the vaginal tract has been approached empirically; some pharmaceuticals containing these microorganisms are available in the United States or Europe or are protected under the patent process or intellectual property rights. There are not enough studies in humans or animals to determine whether their administration can produce some type of collateral or adverse effect. Using Balb/c mice as the experimental model, the object of the present work was to study (1) whether intravaginal administration of human lactobacilli can produce colonization of the tract; (2) whether such administration produces some type of adverse or collateral effect; and (3) whether probiotics are able to stimulate the local immune system. Keeping in mind that hormones can affect the colonization or persistence ability of microorganisms, and with the purpose of having all animals at the same point in the sexual cycle, animals were cycled with estradiol 48 h before inoculation with lactobacilli. They were then inoculated im with hormones 48 h before beginning microorganism inoculations. Later they were intravaginally inoculated with the appropriate dose of each Lactobacillus strains. The animals were sacrificed on different days after inoculation to perform the following studies: 1. Microbiological assays: To determine the number of lactobacilli in the tract (in vaginal washes or in organ homogenates), by plating the samples in selective media containing antibiotic (to differentiate the resident flora from those administered experimentally).
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The aim of this work was to formulate a culture medium of lower cost than conventional laboratory media, in order to simultaneously obtain high amounts of both biomass and bacteriocin of vaginal Lactobacillus salivarius CRL 1328. The... more
The aim of this work was to formulate a culture medium of lower cost than conventional laboratory media, in order to simultaneously obtain high amounts of both biomass and bacteriocin of vaginal Lactobacillus salivarius CRL 1328. The growth assays under different culture conditions were performed by using a 2(8-2) central composite experimental design, with a central point and sixteen additional points. The factors taken into consideration were glucose, lactose, yeast extract, tryptone, ammonium citrate, sodium acetate, MgSO4 and MnSO4. The simultaneous presence of a carbon source (mainly glucose), a nitrogen source (mainly yeast extract) and salts (mainly MnSO4, MgSO4 and sodium acetate) allowed the highest cell biomass and bacteriocin levels to be reached in the experimental design. Through the application of the desirability function, several optimal medium compositions to achieve efficient production of biomass and bacteriocin were predicted. The optimized growth media allow a cost reduction of around 25 to 40% compared with conventional broths. The results obtained represent an advance in the search of the most suitable strategies for the production of bioactive compounds for pharmaceutical products to prevent or treat female urogenital infections.