... M. Rubin, N. Newman, JS Chan, TC Fu, and JT Ross Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720 (Received 28 ... The Ga and Al sources were WA Technologies Series-3 Knudsen cells, and the magnesium... more
... M. Rubin, N. Newman, JS Chan, TC Fu, and JT Ross Lawrence Berkeley Laboratory, University of California, Berkeley, California 94720 (Received 28 ... The Ga and Al sources were WA Technologies Series-3 Knudsen cells, and the magnesium source is a Riber Knudsen cell. ...
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Lafarge Corporation has installed several brands of distributed control systems (DCS) and PLC systems in our North American plants. In the past, the system selection criteria focused on state-of-the-art technology, plant experience and... more
Lafarge Corporation has installed several brands of distributed control systems (DCS) and PLC systems in our North American plants. In the past, the system selection criteria focused on state-of-the-art technology, plant experience and availability of local services. This paper describes why, with the rapid change of technology today, Lafarge has moved away from such a strategy, and why the AEG Geamatics B system was selected for our plant at Joppa, Illinois. The emergence of the so-called open-platform, and the evolution of classical DCS suppliers to PLC platforms made this decision possible. Implementation and key features of the AEG system at our Joppa plant are described. Also, issues concerning a corporate automation vision and the potential benefits resulting from standardization in a large organization are examined
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We have produced 400 ns rf pulses of greater than 500 MW at 11.424 GHz with an rf system designed to demonstrate technology capable of powering a TeV scale electron-positron linear collider (NLC). Power is produced by four 50 MW X-band... more
We have produced 400 ns rf pulses of greater than 500 MW at 11.424 GHz with an rf system designed to demonstrate technology capable of powering a TeV scale electron-positron linear collider (NLC). Power is produced by four 50 MW X-band klystrons run off a common 400 kV solid-state modulator. We present the layout of our system, which includes a dual-moded transmission waveguide system and a dual-moded resonant-line (SLED-II) pulse compression system. Dual-moding of the transmission lines allows power to be directed through a pulse compression path or a bypass path; dual-moding in the pulse compressor allows the delay lines to be about half as long as they otherwise would need to be. We describe the design and performance of various components, including hybrids, directional couplers, power dividers, tapers, mode converters, and loads. These components are mostly overmoded to allow for greater power handling. We also present data on the processing and operation of this system, which is setting high-power records in pulsed rf.
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The LCLS injector is required to provide a 1-nC, 10-ps bunch with a normalized rms transverse projected emittance of less than 1 micron. The LCLS beam is generated and accelerated in a 1.6-cell S-band RF gun at 120 MV/m up to 6 MeV. The... more
The LCLS injector is required to provide a 1-nC, 10-ps bunch with a normalized rms transverse projected emittance of less than 1 micron. The LCLS beam is generated and accelerated in a 1.6-cell S-band RF gun at 120 MV/m up to 6 MeV. The gun is followed by two SLAC 3-m S-band accelerator structures to further accelerate the beam to 135 MeV which moves the beam out of the space-charge dominated regime. In the SLAC S-band structures, the RF power feed is through a single coupling-hole (single-feed coupler) which results in a field asymmetry. The time dependent multipole fields in the coupler induce a transverse kick along the bunch and cause the emittance to increase above the LCLS specification. To meet the stringent emittance requirements for the injector, the single-feed couplers will be replaced by a dual-feed racetrack design to minimize the multipole field effects. We will present detailed studies of the multipole fields in the SLAC linac RF coupler and the improvements with the dual-feed ractrack design using the parallel finite element S-parameter solver S3P.
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During the past five years, there has been an concerted program at SLAC and KEK to develop accelerator structures that meet the high gradient (65 MV/m) performance requirements for the Next Linear Collider (NLC) and Global Linear Collider... more
During the past five years, there has been an concerted program at SLAC and KEK to develop accelerator structures that meet the high gradient (65 MV/m) performance requirements for the Next Linear Collider (NLC) and Global Linear Collider (GLC) initiatives. The design that resulted is a 60-cm-long, traveling-wave structure with low group velocity and 150 degree per cell phase advance. It has an average iris size that produces an acceptable short-range wakefield, and dipole mode damping and detuning that adequately suppresses the long-range wakefield. More than eight such structures have operated at a 60 Hz repetition rate over 1000 hours at 65 MV/m with 400 ns long pulses, and have reached breakdown rate levels below the limit for the linear collider. Moreover, the structures are robust in that the rates continue to decrease over time, and if the structures are briefly exposed to air, the rates recover to their low levels within a few days. This paper presents a summary of the results from this program, which effectively ended last August with the selection of ‘cold’ technology for an International Linear Collider (ILC).
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Page 1. Revista Fitotecnia Mexicana Sociedad Mexicana de Fitogenética, AC somefi@taurus1.chapingo.mx, vagh@colpos.mx ISSN (Versión impresa): 0187-7380 MÉXICO 2005 Luis Sáenz / Ramón Souza / José Luis Chan ...
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Development of micropropagation protocols for Cocos nucifera has progressed slowly. Activated charcoal is included in the culture medium of each protocol, mainly to prevent tissue browning. Charcoal production procedures can affect the... more
Development of micropropagation protocols for Cocos nucifera has progressed slowly. Activated charcoal is included in the culture medium of each protocol, mainly to prevent tissue browning. Charcoal production procedures can affect the properties of different brands. In this study, eight types of activated charcoal were evaluated for their effects on free 2,4-dichlorophenoxyacetic acid level, pH, conductivity, and osmolarity of the culture medium and on the frequency of embryogenic callus induction. Moreover, the effect of particle size of the optimum charcoal type on embryogenic callus development was also studied. Charcoal type had a significant effect on (Y3) culture medium properties. Free 2,4-D was highest in Reactivos y Productos Químicos Finos-containing medium and pH was lowest in MERCK-containing medium. Charcoal type also influenced embryogenic callus induction, with acid washed for plant cell and tissue culture-, DARCO- and United States Pharmacopeia-containing media promoting ~60% embryogenic callus, but with different optimal 2,4-D concentrations. Particle size profiles varied among all charcoal types, although small particle fraction (
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The increasing demand in México for disease resistant coconut palms (Cocos nucifera L.) requires massive multiplication of improved or selected genotypes. This could be achieved through micropropagation. A reproducible micropropagation... more
The increasing demand in México for disease resistant coconut palms (Cocos nucifera L.) requires massive multiplication of improved or selected genotypes. This could be achieved through micropropagation. A reproducible micropropagation protocol via somatic embryogenesis, ...
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Studies on the development of protocols for the clonal propagation, through somatic embryogenesis, of coconut have been reported for the past three decades, mostly using inflorescence explants, but with low reproducibility and efficiency.... more
Studies on the development of protocols for the clonal propagation, through somatic embryogenesis, of coconut have been reported for the past three decades, mostly using inflorescence explants, but with low reproducibility and efficiency. Recent improvements in these respects have been achieved using plumular explants. Here, we report a developmental study of embryogenesis in plumule explants using histological techniques in order to extend our understanding of this process. Coconut plumule explants consisted of the shoot meristem including leaf primordia. At day 15 of culture, the explants did not show any apparent growth; however, a transverse section showed noticeable growth of the plumular leaves forming a ring around the inner leaves and the shoot meristem, which did not show any apparent growth. At day 30, the shoot meristem started to grow and the plumular leaves continued growing., At day 45, the explants were still compact and white in color, but showed partial dedifferentiation and meristematic cell proliferation leading to the development of callus structures with a translucent appearance. After 60 d, these meristematic cells evolved into nodular structures. At day 75, the nodular structures became pearly globular structures on the surface of translucent structures, from which somatic embryos eventually formed and presented well-developed root and caulinar meristems. These results allow better insights and an integrated view into the somatic embryogenesis process in coconut plumule explants, which could be helpful for future studies that eventually could lead us to improved control of the process and greater efficiency of somatic embryo and plantlet formation.
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Agave tequilana stem explants were used to produce adventitious shoots under a set of different water potentials induced by different concentrations of gelrite in the medium. At high water potentials all shoots were vitrified; as the... more
Agave tequilana stem explants were used to produce adventitious shoots under a set of different water potentials induced by different concentrations of gelrite in the medium. At high water potentials all shoots were vitrified; as the medium water potential became more negative the degree of vitrification decreased but the number of shoots per explant also diminished. The enzymes NADH and NAD-GDH (EC. 1.4.1.2) were measured along the water potential gradient. GDH activity was high in the non-vitrified tissues and decreased significantly in the vitrified ones.
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A protocol was developed for coconut regeneration using plumules from mature zygotic embryos as explants, and media with the synthetic growth regulators 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. Evidence for the regeneration... more
A protocol was developed for coconut regeneration using plumules from mature zygotic embryos as explants, and media with the synthetic growth regulators 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine. Evidence for the regeneration process from these tissues occurring through somatic embryogenesis is presented. The somatic embryos were capable of germination, subsequent development into plantlets and successful transfer to the nursery. The yields were larger, nearly twofold for calli and over tenfold for calli bearing somatic embryos, than those previously reported with inflorescence explants. The present protocol thus represents an improvement in time and yield over previous protocols. Even though plumule explants are not the ideal tissue source due to possible genetic heterogeneity, the improvements made here may be applicable to tissues from mature plants. In addition, micropropagation of coconut using plumules is potentially useful when they are obtained from fruit produced from selected parents of outstanding performance, such as those resistant to diseases.
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Coconut is one of the most recalcitrant species to regenerate in vitro. Although previous research efforts using plumule explants have resulted in reproducible somatic embryogenesis, efficiency is only 4 or 10 somatic embryos per plumule... more
Coconut is one of the most recalcitrant species to regenerate in vitro. Although previous research efforts using plumule explants have resulted in reproducible somatic embryogenesis, efficiency is only 4 or 10 somatic embryos per plumule without or with a brassinolide treatment, respectively. In order to increase the efficiency of somatic embryogenesis in coconut, two different approaches were evaluated and reported here: secondary somatic embryogenesis and multiplication of embryogenic callus. Primary somatic embryos obtained from plumule explants were used as explants and formed both embryogenic callus and secondary somatic embryos. The embrogenic calluses obtained after three multiplication cycles were capable of producing somatic embryos. The efficiency of the system was evaluated in a stepwise process beginning with an initial step for inducing primary somatic embryogenesis followed by three steps for inducing secondary somatic embryogenesis followed by three steps for embryogenenis callus multiplication, and finally production of somatic embryos from callus. The total calculated yield from one plumule was 98 000 somatic embryos. Comparing this to the yield obtained from primary somatic embryogenesis results in about a 50 000-fold increase. When compared to the yield previously reported in the literature with the use of a brassinolide treatment, it is about a 10 000-fold increase in yield. The present protocol represents important progress in improvement in the efficiency of coconut somatic embryo production.
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The presence of tyrosine kinase and tyrosine-phosphorylated proteins was investigated in coconut tissues cultured in vitro. In order to study this phenomenon, plumular explants were taken from mature zygotic embryos and cultured in a... more
The presence of tyrosine kinase and tyrosine-phosphorylated proteins was investigated in coconut tissues cultured in vitro. In order to study this phenomenon, plumular explants were taken from mature zygotic embryos and cultured in a medium that induces somatic embryogenesis. Immunoblot analyses of soluble proteins of coconut cultured tissues with a recombinant monoclonal antibody against phosphotyrosine detected protein bands with molecular
Research Interests: Plant Biology, Plant Physiology, Cell line, Tissue culture, Somatic Embryogenesis, and 11 moreSoil sciences, Plant Physiology and Biochemistry, Epidermal Growth Factor, Tyrosine Kinase Inhibitor, In vitro culture, Molecular Mass, Monoclonal Antibody, Amino Acid Sequence, Embryos, Tyrosine Kinase, and Tyrosine Phosphorylation
Somatic embryogenesis involves different molecular events including differential gene expression and various signal transduction pathways. One of the genes identified in early somatic embryogenesis is S OMATIC E MBRYOGENESIS R... more
Somatic embryogenesis involves different molecular events including differential gene expression and various signal transduction pathways. One of the genes identified in early somatic embryogenesis is S OMATIC E MBRYOGENESIS R ECEPTOR-like K INASE (SERK). Cocos nucifera (L.) is one of the most recalcitrant species for in vitro regeneration, achieved so far only through somatic embryogenesis, although just a few embryos could be obtained from a single explant. In order to increase efficiency of this process we need to understand it better. Therefore, the purpose of the present work was to determine if an ortholog of the SERK gene is present in the coconut genome, isolate it and analyze its expression during somatic embryogenesis. The results showed the occurrence of a SERK ortholog referred to as CnSERK. Predicted sequence analysis showed that CnSERK encodes a SERK protein with the domains reported in the SERK proteins in other species. These domains consist of a signal peptide, a leucine zipper domain, five LRR, the Serine-Proline-Proline domain, which is a distinctive domain of the SERK proteins, a single transmembrane domain, the kinase domain with 11 subdomains and the C terminal region. Analysis of its expression showed that it could be detected in embryogenic tissues before embryo development could be observed. In contrast it was not detected or at lower levels in non-embryogenic tissues, thus suggesting that CnSERK expression is associated with induction of somatic embryogenesis and that it could be a potential marker of cells competent to form somatic embryos in coconut tissues cultured in vitro.
Research Interests: Plant Biology, Sequence Analysis, Phylogeny, Tissue culture, Sequence alignment, and 16 moreProtein Kinases, Cocos, Somatic Embryogenesis, Signal Transduction Pathway Models, Molecular cloning, Seeds, SIGNAL PEPTIDE, Amino Acid Sequence, Regional Analysis, Embryos, Embryogenic Callus, PLANT PROTEINS, In vitro regeneration, Differential Gene Expression, Embryo Development, and Molecular Sequence Data
Cardiac catheterization is a well-known cause of infection, both locally at the site of catheter insertion and systemically due to transient bacteremia caused by catheter insertion. The majority of cardiac catheterization-associated... more
Cardiac catheterization is a well-known cause of infection, both locally at the site of catheter insertion and systemically due to transient bacteremia caused by catheter insertion. The majority of cardiac catheterization-associated infections are attributable to staphylococcal ...