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The application of serum interleukin-6 (IL-6) in the diagnosis and prognosis of colorectal cancer (CRC) has been evaluated in many studies, whereas the results were contradictive.The aim of this study was to systematically evaluate this... more
The application of serum interleukin-6 (IL-6) in the diagnosis and prognosis of colorectal cancer (CRC) has been evaluated in many studies, whereas the results were contradictive.The aim of this study was to systematically evaluate this issue.An original study was conducted to explore the diagnostic value of serum IL-6 in CRC. Pubmed, Embase, and Cochrane library databases were searched for eligible studies.For diagnostic meta-analysis, aggregate data (AD) and individual participant data (IPD) meta-analyses were both adopted. The sensitivity and specificity were pooled and a summary receiver-operating characteristic (ROC) curve was constructed. For prognostic meta-analysis, study-specific hazard ratios (HRs) of IL-6 for survival were summarized. Secondary analysis of survival data was performed to synthesize the Kaplan-Meier curves.Total 17 studies (including our study) were included in this meta-analysis. The pooled sensitivity, specificity, and area under curve (AUC) of serum IL-6...
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Background: Human herpes virus type-6 (HHV-6) is increasingly recognised as a febrile agent in children. However, less is known in sub-Saharan African countries, including Sudan.Objective: We investigated the involvement of HHV-6 in... more
Background: Human herpes virus type-6 (HHV-6) is increasingly recognised as a febrile agent in children. However, less is known in sub-Saharan African countries, including Sudan.Objective: We investigated the involvement of HHV-6 in paediatric central nervous system (CNS) infections in Khartoum, Sudan.Methods: Febrile patients aged up to 15 years with suspected CNS infections at Omdurman Hospital for Children from 01 December 2009 to 01 August 2010 were included. Viral DNA was extracted from leftover cerebrospinal fluid (CSF) specimens and quantitatively amplified by real-time polymerase chain reaction (PCR) at Umeå University in Sweden.Results: Of 503 CSF specimens, 13 (2.6%) were positive for HHV-6 (33.0% [13/40 of cases with proven infectious meningitis]). The median thermal cycle threshold for all HHV-6-positive specimens was 38 (range: 31.9–40.8). The median number of virus copies was 281.3/PCR run (1 × 105 copies/mL CSF; range: 30–44 × 103 copies/PCR run [12 × 103 – 18 × 106 c...
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SINV was approved as a causative agent of pogosta disease. The seroprevalence of SINV antibodies for the Finnish population is around 2%, considering the prevalence varies between different regions of Finland. While the seroprevalence of... more
SINV was approved as a causative agent of pogosta disease. The seroprevalence of SINV antibodies for the Finnish population is around 2%, considering the prevalence varies between different regions of Finland. While the seroprevalence of SINV antibodies in Sweden highest in central parts of the country. The annual incidence rate in endemic regions of affected countries ranges from 2.7/100,000 in Finland and 2.9/100,000 in Sweden to 18/100,000 in Northern Karelia. This is the most widely distributed of all known arboviruses, affecting all age groups. This study describes the design and evaluation of a rapid and robust quantitative PCR assay able to detect a wide range of different SINV. Primers with the potential to detect all SINV were designed from conserved regions of all different strains of sindbis virus sequences, as identified from multiple alignments. By using SYBR-green-based quantitative real-time PCR (QPCR) protocols, this QPCR assay is able to detect 50-100 target molecul...
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This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Älvkarleby, Sweden. <br>The dataset is part of a research study led associate professor Anna J. Székely (SLU, Swedish University of... more
This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Älvkarleby, Sweden. <br>The dataset is part of a research study led associate professor Anna J. Székely (SLU, Swedish University of Agricultural Sciences) and her research groups in collaboration with Uppsala Vatten. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.<br>The viral content is concentrated according to the protocol from Ahmed et al. https://doi.org/10.1016/j.scitotenv.2020.139960according to the modified electronegative filtration method. Viral RNA is extracted and SARS-CoV-2 gene copy nr quantified using R-qPCR. Recovery efficiency is measured using Bovine coronavirus (BCoV) as a virus surrogate. Pepper mild mottle virus (PPMoV) is used to correct for variations in water size and flow. For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/#...
This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Uppsala and neighbouring towns Knivsta, Enköping, Östhammar and Älvkarleby.<br>The dataset is part of a research study led... more
This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Uppsala and neighbouring towns Knivsta, Enköping, Östhammar and Älvkarleby.<br>The dataset is part of a research study led associate professor Anna J. Székely (SLU, Swedish University of Agricultural Sciences) and her research groups in collaboration with Uppsala Vatten. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.<br>The viral content is concentrated according to the protocol from Ahmed et al. https://doi.org/10.1016/j.scitotenv.2020.139960according to the modified electronegative filtration method. Viral RNA is extracted and SARS-CoV-2 gene copy nr quantified using R-qPCR. Recovery efficiency is measured using Bovine coronavirus (BCoV) as a virus surrogate. Pepper mild mottle virus (PPMoV) is used to correct for variations in water size and flow. For more information see https://www.c...
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Research Interests:
Modelling of switching pulse width modulated phase-shifted full-bridge zero voltage converter is highly challenging due to several transitional stages involved in a complete switching cycle. The circuit averaging technique has been... more
Modelling of switching pulse width modulated phase-shifted full-bridge zero voltage converter is highly challenging due to several transitional stages involved in a complete switching cycle. The circuit averaging technique has been favored in the past three decades considering phase-shifted full-bridge converter as a buck-derived converter. This modelling approach requires several assumptions that include zero equivalent series resistance of output capacitor, zero leakage inductance and unity transformer turn ratio which results in a less accurate system dynamics. In this paper, a system identification approach is proposed for obtaining a more accurate model of phase-shifted full-bridge PWM switching converter using the input-output test data. The model parameter estimation is implemented in Simulink environment incorporating all circuit parasitic parameters and the resulting system&#39;s frequency response shows a good agreement when compared with reported measured response.
Research Interests: Electrical Engineering, Power Electronics, Mechatronics, Electrical and Electronics, Power Electrical Engineering, and 8 moreMechatronics Engineering, Power Electronics Converter, Power Electronics and Drives, Boost Converter, Dc-Dc Boost Converter, Electrics & mechatronic engineering, Electrical and Electronics Engineering, and Dc DC converters
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Research Interests:
The Middle East respiratory syndrome coronavirus (MERS-CoV) is a life-threatening respiratory disease with a high case fatality rate; however, its risk factors remain unclear. We aimed to explore the influence of demographic factors,... more
The Middle East respiratory syndrome coronavirus (MERS-CoV) is a life-threatening respiratory disease with a high case fatality rate; however, its risk factors remain unclear. We aimed to explore the influence of demographic factors, clinical manifestations and underlying comorbidities on mortality in MERS-CoV patients. Retrospective chart reviews were performed to identify all laboratory-confirmed cases of MERS-COV infection in Saudi Arabia that were reported to the Ministry of Health of Saudi Arabia between 23 April 2014 and 7 June 2016. Statistical analyses were conducted to assess the effect of sex, age, clinical presentation and comorbidities on mortality from MERS-CoV. A total of 281 confirmed MERS-CoV cases were identified: 167 (59.4%) patients were male and 55 (20%) died. Mortality predominantly occurred among Saudi nationals and older patients and was significantly associated with respiratory failure and shortness of breath. Of the 281 confirmed cases, 160 (56.9%) involved ...
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Multiple-input multiple-output (MIMO) direct conversion transceiver (MIMO-DCT) is one of the major advances in recent generation wireless standards that led to the large increase in the data rates and throughput via the use of multiple... more
Multiple-input multiple-output (MIMO) direct conversion transceiver (MIMO-DCT) is one of the major advances in recent generation wireless standards that led to the large increase in the data rates and throughput via the use of multiple antennas on the receiver and transmitter sides. In this paper, the design of a compact miniature and low cost rectangular microstrip patch antenna integrated with hybrid coupler, Wilkinson power divider and single-pole double-throw (SPDT) transmitter/receiver (T/R) switch have been investigated for MIMO direct conversion, or zero IF transceiver system. Different parametric solutions have been optimizing the proposed structure components. The hybrid coupler can act as a phase shifter to provide the necessary characteristics to operate with I/Q signal for direct conversions. The entire proposed structure has been evaluated using a commercial software. The final proposed design has been fabricated and the radiation characteristics have been illustrated using network analyzer to meet the requirements for LTE and communication applications.
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Symbolic execution is widely used for analyzing software behavior, generating test pattern, and finding bugs. However, it is not feasible for large programs. Symbolic execution attempts to explore each path of the program which result in... more
Symbolic execution is widely used for analyzing software behavior, generating test pattern, and finding bugs. However, it is not feasible for large programs. Symbolic execution attempts to explore each path of the program which result in a path explosion for large programs. This paper introduces a framework that makes the symbolic execution practical for the virtual HW models that run on QEMU platform. We describe an approach that can symbolically execute a virtual HW model to automatically generate selective test patterns. We use the constraints-based technique in order to show preferences for the generated test pattern. A native symbolic run of the program along with the constraints will generate test patterns correspond to every possible path. Our technique adds assertion statement into the program to indicate a specific operation mode for the device that the developer pay attention on. The symbolic engine generates test patterns that can derive the program through all feasible paths to reach the assertion. These test patterns can be used to verify same operation mode on the associated HW RTL model.
Research Interests: Computer Science and IEEE
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ABSTRACT This paper proposes a comparison between single-diode model and two-diode model of photovoltaic (PV) module. The main concern of this work is the accuracy, number of unknown parameters, and the execution time for the unknown... more
ABSTRACT This paper proposes a comparison between single-diode model and two-diode model of photovoltaic (PV) module. The main concern of this work is the accuracy, number of unknown parameters, and the execution time for the unknown parameters, under standard test conditions (STC), in each model. The proposed work tests the accuracy of both models under different temperature conditions using two types of solar modules. The accuracy of each model is tested in terms of maximum power and maximum voltage, and compared to justify the pros and cons of each model.
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Abstract A virus causing a severe leafmottle was detected in cocksfoot (Dactylis glomerata L.) m the Taupe area. The disease affected up to 80% of plants at this site but was not detected many other area surveyed. The virus was identified... more
Abstract A virus causing a severe leafmottle was detected in cocksfoot (Dactylis glomerata L.) m the Taupe area. The disease affected up to 80% of plants at this site but was not detected many other area surveyed. The virus was identified on the basis of host range, serology, morphology, and physical properties as cocksfoot mottle virus.
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There is significant interest in the development of methods to create hybrid materials that transform capabilities, in particular for Earth-abundant metal oxides, such as TiO, to give improved or new properties relevant to a broad... more
There is significant interest in the development of methods to create hybrid materials that transform capabilities, in particular for Earth-abundant metal oxides, such as TiO, to give improved or new properties relevant to a broad spectrum of applications. Here we introduce an approach we refer to as 'molecular cross-linking', whereby a hybrid molecular boron oxide material is formed from polyhedral boron-cluster precursors of the type [B(OH)]. This new approach is enabled by the inherent robustness of the boron-cluster molecular building block, which is compatible with the harsh thermal and oxidizing conditions that are necessary for the synthesis of many metal oxides. In this work, using a battery of experimental techniques and materials simulation, we show how this material can be interfaced successfully with TiOand other metal oxides to give boron-rich hybrid materials with intriguing photophysical and electrochemical properties.
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Rift Valley fever virus is an emerging mosquito-borne virus that causes infections in animals and human beings in Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever lead to mass abortions in livestock, but such abortions... more
Rift Valley fever virus is an emerging mosquito-borne virus that causes infections in animals and human beings in Africa and the Arabian Peninsula. Outbreaks of Rift Valley fever lead to mass abortions in livestock, but such abortions have not been identified in human beings. Our aim was to investigate the cause of miscarriages in febrile pregnant women in an area endemic for Rift Valley fever. Pregnant women with fever of unknown origin who attended the governmental hospital of Port Sudan, Sudan, between June 30, 2011, and Nov 17, 2012, were sampled at admission and included in this cross-sectional study. Medical records were retrieved and haematological tests were done on patient samples. Presence of viral RNA as well as antibodies against a variety of viruses were analysed. Any association of viral infections, symptoms, and laboratory parameters to pregnancy outcome was investigated using Pearson's χ(2) test. Of 130 pregnant women with febrile disease, 28 were infected with R...
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To stem the tide of food-borne illnesses, it is important to understand the source of the problem. This volume examines pathogenic bacteria, viruses, protozoan parasites, moulds, and mycotoxins in ...
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An accurate estimation of the series resistance in the PV model is crucial to the prediction of the maximal output power of the PV module, especially under temperature variation. This Paper proposes a theoretical expression to calculate... more
An accurate estimation of the series resistance in the PV model is crucial to the prediction of the maximal output power of the PV module, especially under temperature variation. This Paper proposes a theoretical expression to calculate accurately the value of this resistance. The proposed expression comes from the physical nature of the various elements constructing this resistance, plus the reverse relation of the output power with cell temperature, utilizing manufacturer datasheet only. The proposed expression was tested against experimental measurements and previous work, showing a clear improvement in tracing the series resistance values under varying temperature.
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Sporadic cases of cell-mediated immunity (CMI) in persons exposed to hepatitis C (HCV) but evidently uninfected have been reported. To further define this, we measured CMI in individuals without evidence of HCV infection, that is,... more
Sporadic cases of cell-mediated immunity (CMI) in persons exposed to hepatitis C (HCV) but evidently uninfected have been reported. To further define this, we measured CMI in individuals without evidence of HCV infection, that is, negative for HCV-antibodies (anti-HCV) and RNA, residing in a rural Egyptian community where prevalence of anti-HCV was 24%. Cell-mediated immunity (CMI) measured by interferon-gamma (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay, confirmed by intracellular staining using flow cytometry, against HCV peptides was measured in seronegative individuals with high-risk (HR) and low-risk (LR) exposures to HCV. Thirteen of 71 (18.3%) HR subjects but only 1 of 35 (2.9%) LR subjects had detectable CMI (P = 0.032). These data are compatible with the hypothesis that exposures to HCV may lead to development of HCV-specific CMI without anti-HCV and ongoing viral replication. We speculate induced CMI clears HCV sometimes when anti-HCV is not detectable, and HCV-spe...
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In this study, our objective was to explore the knowledge of Irritable Bowel syndrome (IBS) among university female students in the Kingdom of Saudi Arabia (KSA). A cross-sectional study was conducted where 307 university students were... more
In this study, our objective was to explore the knowledge of Irritable Bowel syndrome (IBS) among university female students in the Kingdom of Saudi Arabia (KSA). A cross-sectional study was conducted where 307 university students were surveyed using a self-administered questionnaire to assess their awareness. The questionnaire was based on the socio-demographic and life-style characteristics of the students to evaluate the prevalence of IBS in the community. About 60% of the population in the age group of 18-20 years are at a high risk of suffering from IBS. However, no significant difference is demonstrated between lifestyle habits such as consumption of fast and spicy foods and physical activities and onset of IBS among the students. Nevertheless, frequent episodes of exercise in a week may reduce the probability of IBS onset. Interestingly, almost half of the student population mentioned that they were taking antibiotics and their sleep was interrupted as they woke up in the mi...
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Background Rodent borne hantaviruses are emerging viruses infecting humans through inhalation. They cause hemorrhagic fever with renal syndrome and hemorrhagic cardiopulmonary syndrome. Recently, hantaviruses have been detected in other... more
Background Rodent borne hantaviruses are emerging viruses infecting humans through inhalation. They cause hemorrhagic fever with renal syndrome and hemorrhagic cardiopulmonary syndrome. Recently, hantaviruses have been detected in other small mammals such as Soricomorpha (shrews, moles) and Chiroptera (bats), suggested as reservoirs for potential pandemic viruses and to play a role in the evolution of hantaviruses. It is important to study the global virome in different reservoirs, therefore our aim was to investigate whether shrews in Sweden carried any hantaviruses. Moreover, to accurately determine the host species, we developed a molecular method for identification of shrews. Method Shrews (n = 198), caught during 1998 in Sweden, were screened with a pan-hantavirus PCR using primers from a conserved region of the large genome segment. In addition to morphological typing of shrews, we developed a molecular based typing method using sequencing of the mitochondrial cytochrome C oxi...
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Alkhurma hemorrhagic fever virus RNA was detected in immature Hyalomma rufipes ticks infesting northward migratory birds caught in the North Mediterranean Basin. This finding suggests a role for birds in the ecology of the Alkhurma... more
Alkhurma hemorrhagic fever virus RNA was detected in immature Hyalomma rufipes ticks infesting northward migratory birds caught in the North Mediterranean Basin. This finding suggests a role for birds in the ecology of the Alkhurma hemorrhagic fever virus and a potential mechanism for dissemination to novel regions. Increased surveillance is warranted.
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Research Interests:
Sporadic cases of cell-mediated immunity (CMI) in persons exposed to hepatitis C (HCV) but evidently uninfected have been reported. To further define this, we measured CMI in individuals without evidence of HCV infection, that is,... more
Sporadic cases of cell-mediated immunity (CMI) in persons exposed to hepatitis C (HCV) but evidently uninfected have been reported. To further define this, we measured CMI in individuals without evidence of HCV infection, that is, negative for HCV-antibodies (anti-HCV) and RNA, residing in a rural Egyptian community where prevalence of anti-HCV was 24%. Cell-mediated immunity (CMI) measured by interferon-gamma (IFN-gamma) enzyme-linked immunospot (ELISPOT) assay, confirmed by intracellular staining using flow cytometry, against HCV peptides was measured in seronegative individuals with high-risk (HR) and low-risk (LR) exposures to HCV. Thirteen of 71 (18.3%) HR subjects but only 1 of 35 (2.9%) LR subjects had detectable CMI (P = 0.032). These data are compatible with the hypothesis that exposures to HCV may lead to development of HCV-specific CMI without anti-HCV and ongoing viral replication. We speculate induced CMI clears HCV sometimes when anti-HCV is not detectable, and HCV-spe...
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Research Interests:
Longitudinal studies of T cell immune responses during viral infections in humans are essential for our understanding of how effector T cell responses develop, clear infection, and provide long-lasting immunity. Here, following an... more
Longitudinal studies of T cell immune responses during viral infections in humans are essential for our understanding of how effector T cell responses develop, clear infection, and provide long-lasting immunity. Here, following an outbreak of a Puumala hantavirus infection in the human population, we longitudinally analyzed the primary CD8 T cell response in infected individuals from the first onset of clinical symptoms until viral clearance. A vigorous CD8 T cell response was observed early following the onset of clinical symptoms, determined by the presence of high numbers of Ki67 + CD38 + HLA-DR + effector CD8 T cells. This response encompassed up to 50% of total blood CD8 T cells, and it subsequently contracted in parallel with a decrease in viral load. Expression levels of perforin and granzyme B were high throughout the initial T cell response and likewise normalized following viral clearance. When monitoring regulatory components, no induction of regulatory CD4 or CD8 T cells...
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Detection of caliciviruses requires high mutation tolerance and throughput. The development of a rational simple, single tube reverse transcription-real-time quantitative PCR (QPCR) technique for human noroviruses (NV) is reported here. A... more
Detection of caliciviruses requires high mutation tolerance and throughput. The development of a rational simple, single tube reverse transcription-real-time quantitative PCR (QPCR) technique for human noroviruses (NV) is reported here. A dual-probe, triple-primer system (NM system) was used for simultaneous detection and preliminary differentiation of NV genogroups in fecal samples. The design was based on a comprehensive analysis of all 1140 NV sequences available in GenBank. A touch-down amplification protocol improved the frequency of detection. The final QPCR was evaluated with 71 fecal samples from outbreak and sporadic cases in Sweden (1997-2004), all calicivirus-positive by electron microscopy. Up to 56 (79 %) were positive. The method is more rational than NV detection methods described previously, and should be a developmental basis for large-scale routine methods for detection of NV.
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Highly conserved nucleotide stretches flanking the cleavage site of the haemagglutinin (HA) gene of influenza type A viruses were utilised for generating PCR amplicons from a broad range of avian influenza viruses (AIV) in a one-step... more
Highly conserved nucleotide stretches flanking the cleavage site of the haemagglutinin (HA) gene of influenza type A viruses were utilised for generating PCR amplicons from a broad range of avian influenza viruses (AIV) in a one-step real-time SYBR Green RT-PCR assay. The nucleotide sequencing of the amplified PCR products simultaneously reveals both the HA subtype and the pathotype of the AIV isolates, as we demonstrated in case of H5 subtype viruses. The specificity of the assay was confirmed by investigating 66 strains of AIV and nine heterologous pathogens, including influenza B, C and various avian pathogenic viruses. This assay enables a general HA subtype identification and pathotype determination of AIV isolates providing a useful alternative tool for avian influenza diagnosis.
Research Interests: Microbiology, Medical Microbiology, Biology, Medicine, real time PCR, and 13 moreVirus, Avian Influenza, Birds, Animals, Method, Journal of Virological Methods, Real Time, Avian Influenza Viruses, influenza A virus, Sensitivity and Specificity, Nucleotides, Nucleotide sequence, and reverse transcriptase polymerase chain reaction
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Research Interests:
Hantaviruses are endemic in most parts of the world and cause hundreds of thousand human cases of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS) annually throughout Eurasia and the Americas.... more
Hantaviruses are endemic in most parts of the world and cause hundreds of thousand human cases of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS) annually throughout Eurasia and the Americas. They are zoonotic viruses, most commonly transmitted to humans by aerosolized rodent excreta. New hantaviruses are frequently discovered in previously unknown reservoir species and geographic areas. Consequently, there is a need to improve hantavirus diagnostics. This paper describes the design and evaluation of a rapid and robust quantitative real-time PCR (QRT-PCR) assay able to detect a wide range of hantaviruses. Primers with the potential to detect different hantaviruses were designed from conserved regions of different hantavirus L segments, as identified from multiple sequence alignments. By using SYBR-green-based QRT-PCR 100-1000 target molecules of in vitro produced RNA and less than 100 copies of hantavirus RNA from different hantavirus clades and regions of the world were detected. When using the assay on clinical samples from patients with acute HFRS, Puumala hantavirus (PUUV) RNA was confirmed in all previously positive samples. Notably, the broad reacting L-segment QRT-PCR also detected viral RNA in HFRS patient samples, previously negative by a QRT-PCR targeting the S segment of PUUV. This novel assay provides a powerful tool for diagnosis of hantaviruses from different clades and regions and may also be useful in surveys with the purpose of finding new hantaviruses in rodent or insectivore species.
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Research Interests:
A PCR assay that covers animal and human influenza A, B and C viruses, ie, most of Orthomyxoviridae, is needed. Influenza types are distinguished based on differences in the nucleoprotein (NP) present in the virus. Conserved NP regions... more
A PCR assay that covers animal and human influenza A, B and C viruses, ie, most of Orthomyxoviridae, is needed. Influenza types are distinguished based on differences in the nucleoprotein (NP) present in the virus. Conserved NP regions were therefore used to ...
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A PCR-enhanced immunoassay (PIA) to detect enterovirus (EV) immunoglobulin M (IgM) for diagnosis of recent EV infection was recently developed. This test was compared with another EV IgM capture technique, the solid-phase reverse... more
A PCR-enhanced immunoassay (PIA) to detect enterovirus (EV) immunoglobulin M (IgM) for diagnosis of recent EV infection was recently developed. This test was compared with another EV IgM capture technique, the solid-phase reverse immunosorbent test (SPRIST). Fourteen of 43 serum samples from aseptic meningitis patients were positive by PIA, whereas 10 were positive by SPRIST. One of 39 control serum samples was weakly positive by PIA. A single-serum-dilution real-time PCR-based PIA for EV IgM (quantitative PIA [QPIA]) was also developed and evaluated against PIA, SPRIST, an EV IgM radioimmunoassay (RIA), and clinical data. A mixture of 12 EVs was used as the antigen. Results from investigating four groups of serum samples were as follows. (i) The nine PIA-positive serum samples in group 1 were all positive by QPIA. (ii) Group 2 consisted of 59 serum samples from aseptic meningitis patients. Nineteen of 30 serum samples (63%) taken at hospital admission were positive by QPIA. Of thes...
Research Interests: Immunology, Biology, Medicine, Cell Culture, Quantitative PCR, and 15 morereal time PCR, Humans, Diabetes mellitus, Polymerase Chain Reaction, Dilated cardiomyopathy, Clinical Data, Group, Sensitivity and Specificity, LABORATORY DIAGNOSIS, Enzyme Linked Immunosorbent Assay, Radioimmunoassay, Upper respiratory tract, immunoglobulin M, Hospital Admission, and Antiviral Therapy
Background: Enteroviruses (EVs) are significant human pathogens. Rapid and sensitive diagnostic techniques are desirable. Objectives: To develop a quantitative single-tube real-time reverse transcription-polymerase chain reaction (RT-PCR)... more
Background: Enteroviruses (EVs) are significant human pathogens. Rapid and sensitive diagnostic techniques are desirable. Objectives: To develop a quantitative single-tube real-time reverse transcription-polymerase chain reaction (RT-PCR) for human enterovirus ribonucleic acid ...
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Research Interests: Green Chemistry, Archives, Biology, Fluorescent Dyes and Reagents, Medicine, and 15 moreBiological Sciences, Phylogeny, Humans, Animals, Primer design, Real Time, Degeneration, Reproducibility of Results, Base Sequence, Open Reading Frame, High Sensitivity, Organic Chemicals, Molecular Sequence Data, fluorescent dyes, and Medical and Health Sciences
Detection of caliciviruses requires high mutation tolerance and throughput. The development of a rational simple, single tube reverse transcription-real-time quantitative PCR (QPCR) technique for human noroviruses (NV) is reported here. A... more
Detection of caliciviruses requires high mutation tolerance and throughput. The development of a rational simple, single tube reverse transcription-real-time quantitative PCR (QPCR) technique for human noroviruses (NV) is reported here. A dual-probe, triple-primer system (NM system) was used for simultaneous detection and preliminary differentiation of NV genogroups in fecal samples. The design was based on a comprehensive analysis of all 1140 NV sequences available in GenBank. A touch-down amplification protocol improved the frequency of detection. The final QPCR was evaluated with 71 fecal samples from outbreak and sporadic cases in Sweden (1997-2004), all calicivirus-positive by electron microscopy. Up to 56 (79 %) were positive. The method is more rational than NV detection methods described previously, and should be a developmental basis for large-scale routine methods for detection of NV.