Sugarcane bagasse (Saccharum officinarum L.) is a readily available waste product of cane-sugar processing. The major components of bagasse are cellulose and hemicellulose. The objective of the research was to produce bio-ethanol from... more
Sugarcane bagasse (Saccharum officinarum L.) is a readily available waste product of cane-sugar processing. The major components of bagasse are cellulose and hemicellulose. The objective of the research was to produce bio-ethanol from hydrolizate of sugarcane (Saccharum officinarum L.) bagasse which hydrolyzed by combination of acid and enzymeand further fermented by three types of microorganisms, respectively P. stipitis CBS 5773, or S. Cerevisiae D1/P3GI, or Z. mobilis 0056 FNCC. The experiment employed descriptive analyses in triplicates. The result were as follows: effectiveness of Z. mobilis 0056 FNCC was highest for producing bio-ethanol, respectively 18.99 g/L within 3 hours. S. cerevisiae D1/P3GI produced 17.05 g/L bio-ethanol within 12 hours, and P. stipitis CBS 5773 13.03 g/L bioethanol within 24 hours.
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Telah dilakukan penelitian degradasi minyak pelumas bekas oleh bakteri asal tanah yang terkontaminasi minyak pelumas bekas dalam media air tawar dan air laut. Penelitian ini bertujuan untuk memperoleh isolat bakteri yang mempunyai... more
Telah dilakukan penelitian degradasi minyak pelumas bekas oleh bakteri asal tanah yang terkontaminasi minyak pelumas bekas dalam media air tawar dan air laut. Penelitian ini bertujuan untuk memperoleh isolat bakteri yang mempunyai kemampuan mendegradasi tinggi terhadap minyak pelumas bekas dalam media air tawar dan air laut. Bakteri hasil isolasi diidentifikasi dan diseleksi terhadap minyak pelumas bekas. Hasil seleksi diaklimatisasi untuk mendapatkan bakteri yang akan diuji pada media pelarut air laut. Hasil seleksi dan aklimatisasi memperlihatkan Arthobacter sp., Bacillus sp., dan Pseudomonas sp. mempunyai degradasi yang tinggi. Uji biodegradasi dilakukan dalam media Bushnell-Haas Gram Mineral dengan pelarut air tawar dan air laut dengan bakteri uji berupa kultur tunggal dan campuran. Hasil identifikasi ditemukan 10 isolat bakteri. Degradasi terbesar pada air laut adalah kultur tunggal Bacillus sp. (52.74%), sedangkan pada air tawar adalah pada kultur campuran Arthrobacter sp., Ba...
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The research of chelation ability on two types of granuls formulation of sappan wood (Caesalpinia sappan, L) extracts on iron-overloaded rats (Rattus norvegicus, L.) has been done. The aims of the study were to determine the effective of... more
The research of chelation ability on two types of granuls formulation of sappan wood (Caesalpinia sappan, L) extracts on iron-overloaded rats (Rattus norvegicus, L.) has been done. The aims of the study were to determine the effective of formulation and the dose of granulated wooden cup extract to reduce the excess iron in rats indicating the condition of iron-overloaded in thalassemic patient due to blood transfusion. The research has been done using completely randomized design with 11 groups and three replications. Eight groups were administered with two formulations at dose of 0,100,200 and 400 mg/kg bw for each formula and the deferipron was taken for control groups. Both groups were given iron dextran. Parameters of iron state measured were the levels of ferritin, and transferin. Data were analyzed with ANOVA and Duncan Multiple Test Distance. Results showed the administration of iron dextran at a dose of 60 mg/kg bw/day caused excess iron in rats which increased ferritin leve...
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Manufacture of crude palm oil (CPO) is one of Indonesia's industrial agro commodities that support the Indonesian economy. The increase in CPO production will increase the production of waste palm oil (Elaeis guinensis Jacq.). Palm... more
Manufacture of crude palm oil (CPO) is one of Indonesia's industrial agro commodities that support the Indonesian economy. The increase in CPO production will increase the production of waste palm oil (Elaeis guinensis Jacq.). Palm oil mill wastewater has high potential to pollute the waters. This is due to palm oil wastewater containing high levels of COD (Chemical Oxygen Demand), and BOD (Biochemical Oxygen Demand), TSS (total suspended solid), containing solids of crude Palm oil, smelling, dark brown color, contain high levels of oils, fats and proteins as well as the rest of the cooking pressed palm fruit bunches. The purpose of this study was to determine the ability of the consortium of Trichoderma viride, Phanerochaete chrysosporium and Bacillus sp. and effective dose inoculums in degrading oil palm wastewater. This study used an experimental method to completely randomized design (CRD) factorial with two factors and three replications. Factor 1 consortium type are c1 (Ba...
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... Untuk mengetahui aktivitas SOD pada berbagai tanaman sayuran, buah-buahan, rempah, bumbu dan ... Sebelum dilakukan uji aktivitas SOD setiap jenis tanaman diekstraksi dengan cara maserasi. ... rotary vacuum evaporator, sehingga bebas... more
... Untuk mengetahui aktivitas SOD pada berbagai tanaman sayuran, buah-buahan, rempah, bumbu dan ... Sebelum dilakukan uji aktivitas SOD setiap jenis tanaman diekstraksi dengan cara maserasi. ... rotary vacuum evaporator, sehingga bebas etanol dan ekstrak yang diperoleh ...
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The modifying effects of dietary feeding of zerumbone isolated from Zingiber zerumbet on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were investigated in male F344 rats. Expression of cyclooxygenase... more
The modifying effects of dietary feeding of zerumbone isolated from Zingiber zerumbet on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were investigated in male F344 rats. Expression of cyclooxygenase (COX)-2 in colonic mucosa exposed to AOM and/or zerumbone was also assayed. In addition, we assessed the effects of zerumbone on cell proliferation activity of crypts by counting silver-stained nucleolar organizer regions protein (AgNORs) in colonic cryptal cell nuclei. To induce ACF rats were given three weekly subcutaneous injections of AOM (15 mg/kg body weight). They were also fed the experimental diet containing 0.01% or 0.05% zerumbone for 5 weeks, starting one week before the first dosing of AOM. AOM exposure produced 84+/-13 ACF/rat at the end of the study (week 5). Dietary administration of zerumbone caused reduction in the frequency of ACF: 72+/-17 (14% reduction) at a dose of 0.01% and 45+/-18 (46% reduction, p<0.001) at a dose of 0.05%. Feeding of zerumbone significantly reduced expression of COX-2 and prostaglandins in colonic mucosa. Zerumbone feeding significantly lowered the number of AgNORs in colonic crypt cell nuclei. These findings might suggest possible chemopreventive ability of zerumbone, through suppression of COX-2 expression, cell proliferating activity of colonic mucosa, and induction of phase II detoxification enzymes in the development of carcinogen-induced ACF.
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Research has been carried out on the encapsulation of probiotics of the genus Bacillus and Lactobacillus using rice flour, maltodextrin, and talc as carriers. The purpose of this study was to obtain a type of carrier material that can... more
Research has been carried out on the encapsulation of probiotics of the genus Bacillus and Lactobacillus using rice flour, maltodextrin, and talc as carriers. The purpose of this study was to obtain a type of carrier material that can maintain the character of probiotics for fishfeed in aquaculture activity. The research method used is the experimental method, and the data were analyzed descriptively and experi-mentally. The experimental method used a completely randomized design with a factorial pattern. The results showed that talc can main-tain antimicrobial activity against E. coli (16.3 mm), S. typhimurium (17.3 and 13.8 mm) and coaggregation (12.9% and 15.01%). Rice flour can maintain antimicrobial activity against E. coli (20 mm), S. typhimurium (11.7 mm), and coaggregation (14.5%). Maltodextrin can maintain antimicrobial activity against E. coli (20.7 and 17.2 mm), S. typhimurium (19.8 and 20.8 mm) and coaggregation (8.8 % and 17.35%).
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Probiotics have been utilized as an option to treat problems brought on by safe and ecologically friendly pathogenic microorganisms in shrimp and fish farming operations. Probiotics are currently available in powder form (dry form). Using... more
Probiotics have been utilized as an option to treat problems brought on by safe and ecologically friendly pathogenic microorganisms in shrimp and fish farming operations. Probiotics are currently available in powder form (dry form). Using spray drying technology, dry probiotics are produced, with the finished result being a stable powder. In order to regulate the proliferation of harmful bacteria in the digestive tract, these bacteria use a competitive mechanism. Bacillus sp., Saccharomyces cereviceae, and Lactobacillus sp. are among the commonly researched probiotic microorganisms. Microorganisms are blended with a number of carriers, including maltodextrin, wheat flour, and rice flour. The carrier material's main goal is to increase storage process survival. Numerous studies have demonstrated that the viability and development of the probiotic microorganisms included in each carrier material varied. The enclosed substance exhibits highly varied microbial growth. The maximum nu...
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Feed is a very important factor in vannamei shrimp culture. The initial stage of stocking is the cultivation stage requiring a higher percentage of feed. The application of probiotics in intensive shrimp culture is known to have a... more
Feed is a very important factor in vannamei shrimp culture. The initial stage of stocking is the cultivation stage requiring a higher percentage of feed. The application of probiotics in intensive shrimp culture is known to have a significant effect on the survival of vannamei shrimp. Encapsulation can increase the viability of probiotic bacteria compared to free cells without encapsulation. Research has been carried out on the provision of encapsulated probiotic consortia in vannamei shrimp culture. The purpose of this study was to observe the effect of the selected probiotic consortium on the performance of vannamei shrimp. The research method used is the experimental method and the data obtained were analyzed descriptively. The observed parameters in this study were specific growth rate, survival rate and feed conversion ratio. The results showed that the fifth consortium of Bacillus and Lactobacillus bacteria could increase the growth rate of weight and length of vannamei shrimp...
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This study aims to obtain raw materials as an effective carrier for starter inoculum consortium of Bacillus coagulans B. licheniformis, B. subtilis, and Pseudomonas sp. and contact time for bioremediation of polluted water from the river... more
This study aims to obtain raw materials as an effective carrier for starter inoculum consortium of Bacillus coagulans B. licheniformis, B. subtilis, and Pseudomonas sp. and contact time for bioremediation of polluted water from the river Cimuka. The study was conducted by using the experimental method with completely randomized design (CRD) with two-factor factorial 9x9. The first factor is the carrier material (P) consisting of 9 level and the second factor is the contact time (W) consisting of 9 level. Parameters measured were BOD, COD, TSS, and ammonia. The results showed that bioremediation by a consortium of bacteria Bacillus coagulans, B. licheniformis, B. subtilis, and Pseudomonas sp. the carrier material alginate-starch with a concentration of 1% at the time bioremediation 14 days can reduce levels of BOD by 81.49%, 81.44% COD, TSS 75%, and 35.82% ammonia by bacteria populations reach 2,89x1013 CFU/ml. The results also show that the encapsulation can maintain the viability o...
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Tujuan dari penelitian ini adalah untuk mengetahui efektifitas hidrolisis kombinasi asam sulfat dan enzim terhadap hasil hidrolisat gula pereduksi. Penelitian dilakukan secara eksperimental, dan data yang diperoleh dianalisis secara... more
Tujuan dari penelitian ini adalah untuk mengetahui efektifitas hidrolisis kombinasi asam sulfat dan enzim terhadap hasil hidrolisat gula pereduksi. Penelitian dilakukan secara eksperimental, dan data yang diperoleh dianalisis secara deskriptif. Hidrolisis secara kimia menggunakan larutan asam sulfat (H2S0 4 ) 6M, pH 1,0-2,0, diinkubasi pada suhu 120°Cselama 60 menit. Hidrolisis lanjutan secara enzimatik dengan menggunakan enzim α-amylase dosis 0,17 I-Il/gdosis , enzim hemiselulase dosis 0.0003 g / g), enzim selulase dosis 0,55 µl/g) dan enzim amiloglukosidase 0,37 µl/g.Dari hasil penelitian dapat diketahui bahwa hidrolisis dengan asam sulfat 6 M dihasilkan konsentrasi gula pereduksi sebesar 22,6 % dengan DE ( Dextro s e /E q u i v a l e nt ) sebesar 28,63 %. Selanjutnya penggunaan hidrolisis enzimatik dapat meningkatkan konsentrasi gula pereduksi sebesar 53, 28 % dan nilai DE hingga 68, 52% KataKunci: Hidrolisis, Sagu, Selulosa, Hemiselulosa, α-amylase, amiloglukosidase, selulase
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This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater... more
This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater origin Cisirung WWTP. This study uses an experimental method completely randomized design (CRD), which consists of two treatment factors (8x8 factorial design). The first factor is a consortium of bacteria (K), consisting of 8 level factors (k1, k2, k3, k4, k5, k6, k7, and k8). The second factor is the time (T), consisting of a 7 level factors (t0, t1, t2, t3, t4, t5, t6, and t7). Test parameters consist of BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand), TSS (Total Suspended Solid), Ammonia and Population of Microbes during bioremediation. Data were analyzed by ANOVA, followed by Duncan test. The results of this study showed that the consortium of Bacillus pumilus, Bacillus subtilis, Bacillus coagulans, Nitrosomonas sp., and Pseudomona...
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Helicobacter pylori (H. pylori) commonly found in patients with chronic gastritis. Despite of standard medical treatment with antacids and antibiotics, Leuconostoc mesenteroides is one of bacteria that has potential as probiotic for anti... more
Helicobacter pylori (H. pylori) commonly found in patients with chronic gastritis. Despite of standard medical treatment with antacids and antibiotics, Leuconostoc mesenteroides is one of bacteria that has potential as probiotic for anti H. pylori. This study was conducted to characterize the probiotic bacteria from cabbage (Brassica oleracea L.) as an anti H. pylori. Identification of bacteria were observed with the API test. The characterizations of anti H. pylori probiotics were studied using the acidic pH resistance tests, the bile salts resistance test, the anti microbial test (inhibition zone) and the probiotic bacteria adhesion test against H. pylori. This study was a descriptive and experimental method with a complete randomized design factorial pattern as the research design. The results were analyzed by ANOVA (α.05 ) and significant results were tested with Duncans multiple range (α.05). The parameters measured were the adhesion ability of probiotic bacteria against H. pyl...
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This research aims was to determine the quality improvement in the nutritional value of cassava (Manihot esculenta Crantz) tuber skin waste through the fermentation process by cellulolytic microbial consortium of Aspergillus tamari,... more
This research aims was to determine the quality improvement in the nutritional value of cassava (Manihot esculenta Crantz) tuber skin waste through the fermentation process by cellulolytic microbial consortium of Aspergillus tamari, Bacillus megaterium and Bacillus mycoides. The usefulness of this research is to improve the quality of the nutritional value of cassava tuber skin waste that can be used as herbivorous fish feed material. The research was conducted in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. The experiment was conducted with experimental methods, while the data were analyzed using the T test. Fermentation was carried out for 7 days by the method of solid substrate. The parameters measured included 1) content of crude protein, crude fat, water, ash and energy using proximate analysis, 2) levels of cyanide acid (HCN) using AOAC method, 3) content of lignin, cellulose and hemicelluloses usi...
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Pengaruh Konsorsium Bakteri (Bacillus subtilis, B. pumilus, B. circulans, Nitrosomonas sp. dan Paenibacillus amyloliticus) dalam Berbagai Bahan Pembawa (alginat-susu skim, alginat-gelatin dan serbuk baglog-serbuk talek) Serta Waktu Kontak Terhadap Kemampuan Bioremediasi Perairan Asal Sungai Cimukamore
Penelitian untuk mendapatkan konsorsium bakteri dalam bahan pembawa yang efektif dalam proses bioremediasiperairan asal sungai Cimuka telah dilakukan. Metode yang digunakan dalam penelitian ini adalah metode deskriptifdan metode... more
Penelitian untuk mendapatkan konsorsium bakteri dalam bahan pembawa yang efektif dalam proses bioremediasiperairan asal sungai Cimuka telah dilakukan. Metode yang digunakan dalam penelitian ini adalah metode deskriptifdan metode eksperimental. Metode deskriptif digunakan pada tahap pengukuran parameter pH dan suhu. Metodeeksperimental yang digunakan yaitu Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktorial dan tigapengulangan untuk parameter jumlah populasi bakteri, amoniak dan TSS. Faktor I adalah kombinasi konsorsiumbakteri dalam bahan pembawa (P) dan faktor II adalah waktu kontak (T). Sedangkan untuk parameter DO, BOD danCOD digunakan analisis variansi satu faktorial, yaitu faktor kombinasi konsorsium bakteri dalam bahan pembawa(P). Hasil penelitian menunjukan bahwa bioremediasi oleh konsorsium bakteri Bacillus subtilis, B. circulans,B. pumillus dan Nitrosomonas sp. dalam kombinasi bahan pembawa alginat dan susu skim dengan konsentrasi1% mampu menurunkan kadar BOD 86.85%, COD 87%, amoniak 67.51% dan TSS 87.5% dan meningkatkanDO 74.85% serta mempertahankan viabilitas konsorsium bakteri yaitu sebesar 4.2x10 CFU/ml pada hari ke14.Kombinasi dari bahan pembawa alginat dan susu skim yang berfungsi melindungi konsorsium bakteri darilingkungan yang tercemar, mengontrol pelepasan konsorsium bakteri ke dalam air dan mempertahankan viabilitaskonsorsium bakteri dengan kemampuan dari konsorsium bakteri Bacillus subtilis, B. circulans, B. pumillus danNitrosomonas sp. dalam menghasilkan berbagai enzim untuk bioremediasi membuat formula tersebut mampudengan baik dalam mengurai bahan organik perairan Sungai Cimuka
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BACKGROUND: Iron-overload cardiomyopathy (IOC) is a major comorbidity in patients with chronic repetitive blood transfusion due to myocardial iron uptake that facilitated by calcium channels. As cardiac compensatory mechanism to IOC, we... more
BACKGROUND: Iron-overload cardiomyopathy (IOC) is a major comorbidity in patients with chronic repetitive blood transfusion due to myocardial iron uptake that facilitated by calcium channels. As cardiac compensatory mechanism to IOC, we hypothesized the cardiac calcium channels expression would be increased and involved in cardiomyopathy progressivity. This study was aimed to investigate the gene expression of calcium channels in the heart of the iron overload mice model.METHODS: Mice were divided into three groups according to iron administration doses 0, 0.1, and 0.3 mg/day. Systolic blood pressure (SBP), diastolic blood pressure (DBP), and mean arterial pressure (MAP) were measured for the representation of cardiovascular outcomes. The heart tissues were harvested. Further mRNA levels of L-type calcium channels (LTCCs) and T-type calcium channels (TTCCs) were examined using semi-quantitative PCR. The expressions of cardiac calcium channels and blood pressure among the three group...
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Bacillus and Lactobacillus are bacteria that can be used in dry-stock probiotics in vannamei shrimp feed. In this study, it was performed to encapsulate Bacillus and Lactobacillus probiotic bacteria in several carrier materials, including... more
Bacillus and Lactobacillus are bacteria that can be used in dry-stock probiotics in vannamei shrimp feed. In this study, it was performed to encapsulate Bacillus and Lactobacillus probiotic bacteria in several carrier materials, including rice flour, talc, and maltodextrin. The purpose of this study was to determine the type of carrier material that can provide the best probiotic character in the encapsulation process which will then be used in vannamei shrimp feed. The method used in this study is experimental method. The study was conducted with the preparation stage and the initial probiotic characterization test. Furthermore, Bacillus and Lactobacillus probiotic encapsulation process used spray drying method to produce powder inoculum, as well as advanced characterization test of bacteria that have been encapsulated. The parameters observed were the viability, resistance to temperature, pH and bile salts. Data were analyzed using ANAVA and Duncan Multiple Range Test. The results...
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This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater... more
This study was conducted in order to determine the ability of bacterial consortium: Bacillus coagulans, Bacilus licheniformis, Bacillus pumilus, Bacillus subtilis, Nitrosomonas sp., and Pseudomonas putida in bioremediation of wastewater origin Cisirung WWTP. This study uses an experimental method completely randomized design (CRD), which consists of two treatment factors (8x8 factorial design). The first factor is a consortium of bacteria (K), consisting of 8 level factors . The second factor is the time (T), consisting of a 7 level factors (t0, t1, t2, t3, t4, t5, t6, and t7
Spodoptera litura ideal untuk digunakan sebagai inang pengganti pada produksi Helicoverpa armigera Nuclear Polyhedrosis Virus. Hal ini karena ukuran tubuh larva S. litura lebih besar daripada ukuran tubuh larva H. armigera dan larva S.... more
Spodoptera litura ideal untuk digunakan sebagai inang pengganti pada produksi Helicoverpa armigera Nuclear Polyhedrosis Virus. Hal ini karena ukuran tubuh larva S. litura lebih besar daripada ukuran tubuh larva H. armigera dan larva S. litura lebih mudah dipelihara secara berkelompok, hingga penyebaran virus akan lebih cepat tersebar antar individu larva. Pada penelitian ini dilakukan produksi HaNPV pada larva S. litura instar empat yang dikelompokan sebanyak 10 ekor, 20 ekor, 30 ekor, 40 ekor, 50 ekor dan 60 ekor. Kelompok larva S. litura diinfeksi virus secara oral dengan cara memberi pakan campuran 1 ml suspensi HaNPV dengan konsentrasi 4 X 105 polihedra/ml yang ditambahkan pada 30 gram pipilan jagung manis. Selanjutnya, HaNPV dipanen pada hari ke tiga setelah larva diinfeksi. Semua polihedra virus dari kadaver larva dalam satu kelompok dihitung, setelah sediaan virus dimurnikan menggunakan metode Indrayani (1993) yang telah dimodifikasi. Hasil penelitian menunjukkan bahwa S. lit...
Research has been carried out to obtain an effective bacterial consortium in the biodegradation of domestic wastewater WWTP Bojongsoang. The method used in this study is an experimental method to completely randomized design (CRD)... more
Research has been carried out to obtain an effective bacterial consortium in the biodegradation of domestic wastewater WWTP Bojongsoang. The method used in this study is an experimental method to completely randomized design (CRD) consisting of 2 factors and repeated 3 times. The first factor is the type of bacterial consortia, these were (k1) Consortium of Bacillus subtilis, Paenibacillus amylolyticus, Nitrosomonas, and Bacillus circulans, (k2) Consortium of Paenibacillus amylolyticus, Bacillus Nitrosomonas circulans, and Bacillus pumilus; (k3) Consortium of Bacillus subtilis, Nitrosomonas, Bacillus circulans and Bacillus pumilus; (k4) of domestic wastewater was heated; (k5) of domestic wastewater was not heated. The second factor is the time of bioremediation (T) of consisting of 8 levels, namely (t0) initial day, (t1) day 4, (t2) day 8, (t3) Day 12, (t4) Day 16 (t5) Day 20, (t6) Day 25. The results showed that the bioremediation by the consortium of Bacillus subtilis, Nitrosomona...
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The purpose of this study was to obtain effective dose of hydrolase enzyme (α-amylase, hemicellulase, cellulase and amyloglucosidase) to produce highest levels of reducing sugars and dextrose equivalent (DE), and also to obtain the best... more
The purpose of this study was to obtain effective dose of hydrolase enzyme (α-amylase, hemicellulase, cellulase and amyloglucosidase) to produce highest levels of reducing sugars and dextrose equivalent (DE), and also to obtain the best microbial consortium in the fermentation of cellulose from pulp waste to produce bioethanol. This research used descriptive and experimental methods. The descriptive method was used in the optimization of hydrolase enzyme dosage, while the experimental method was used in cellulose of reject pulp fermentation. Parameters used in this study consist in ethanol content, reducing sugar content, microbial population and DE. Our results showed that the highest levels of reducing sugars in the optimization process of α-amylase enzyme was obtained at a dose of 0,52 μL g1whereas hemicellulase enzyme was at a dose of 2/3 or 0.00067 g g-1 and cellulase as well as amyloglucosidase was at a dose of 1 + 1 or 0.83 μL g-1 and 0,56 μL g-1, respectively. Additionally, ...
Dalam buku ini dipaparkan mengenai teknik-teknik dasar kerja dilaboratorium mikrobiologi, cara pemeriksaan rutin mikrobiologis, aplikasi dasar yang digunakan sebagai acuan untuk analisis bidang mikrobiologi.
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The Research was focussed on the effectiveness of a bacterial consortium to the bioremediation of industrial waste water containing dye textiles blue Remazol, in which it has successfuly been done. The aim of this study was to determine... more
The Research was focussed on the effectiveness of a bacterial consortium to the bioremediation of industrial waste water containing dye textiles blue Remazol, in which it has successfuly been done. The aim of this study was to determine the effectiveness of consortium of bacteria on bioremediation of textile waste water which contains Remazol blue dye. The process was to clearly find out how the bacterial consortiums capability in degrading the textile industry wastewater in which the blue dye Remazol is contained. The research was carried out experimentally using Randomized Complete Block and some factorial treatments. These two treatment factors comprises of two types, they are: a). The type of microbial consortium consisting of the Consortium of Bacillus coagulans, B. pumilus, B. subtilis, and Nitrosomonas sp.; consortium of bacteria Bacillus licheniformis, B. pumilus, B. subtilis, Pseudomonas putida, and Nitrosomonas sp and a consortium of Paenibacillus amylolyticus bacteria, Ni...
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This study aims to determine the effective dose and the granule formulation of sappan wood (Caesalpinia sappan L.) extract (SWE) to increase the antioxidant activity in blood serum in excessive iron condition. This study was complete... more
This study aims to determine the effective dose and the granule formulation of sappan wood (Caesalpinia sappan L.) extract (SWE) to increase the antioxidant activity in blood serum in excessive iron condition. This study was complete random design with 11 treatments and was repeated three times. Male wistar rat (R. norvegicus L.) of 200 g, was given with iron dextran to induce a state of iron excessed. A total of eight groups were then given by two types of granule formulations, and each granule formulation consisting of SWE doses of 0, 100, 200, and 400 mg / kg bw, 3 treatment groups: group that was given by Iron Dextran, distilled water, and deferiprone as a comparison. The study was conducted over 15 days, the parameters observed including: activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA). The results showed that administration of Iron Dextran 60 mg / kg caused increase of iron level which also caused the incre...
This study aims to determine the effective dose and the granule formulation of sappan wood (Caesalpinia sappan L.) extract (SWE) to increase the antioxidant activity in blood serum in excessive iron condition. This study was complete... more
This study aims to determine the effective dose and the granule formulation of sappan wood (Caesalpinia sappan L.) extract (SWE) to increase the antioxidant activity in blood serum in excessive iron condition. This study was complete random design with 11 treatments and was repeated three times. Male wistar rat (R. norvegicus L.) of 200 g, was given with iron dextran to induce a state of iron excessed. A total of eight groups were then given by two types of granule formulations, and each granule formulation consisting of SWE doses of 0, 100, 200, and 400 mg / kg bw, 3 treatment groups: group that was given by Iron Dextran, distilled water, and deferiprone as a comparison. The study was conducted over 15 days, the parameters observed including: activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and the levels of malondialdehyde (MDA). The results showed that administration of Iron Dextran 60 mg / kg caused increase of iron level which also caused the incre...
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Andriani Y, Safitri R, Rochima E, Fakhrudin SD. 2017. Characterization of Bacillus subtilis and B. licheniformis potentials as probiotic bacteria in Vanamei shrimp feed (Litopenaeus Vannamei Boone, 1931). Nusantara Bioscience 9: 188-193.... more
Andriani Y, Safitri R, Rochima E, Fakhrudin SD. 2017. Characterization of Bacillus subtilis and B. licheniformis potentials as probiotic bacteria in Vanamei shrimp feed (Litopenaeus Vannamei Boone, 1931). Nusantara Bioscience 9: 188-193. This study aimed to characterize the Bacillus subtilis and Bacillus licheniformis potentials as probiotic bacteria in Vanamei shrimp feed (Litopenaeus vannamei Boone, 1931). A two-phase experimental descriptive method in a laboratory scale was applied in this study. The first phase was the bacterial growth curve observation of B. subtilis and B. licheniformis. The second phase was the characterization of the two bacteria in terms of their tolerance to an acidic condition, temperature, and bile salt concentration. The measured parameter was the number of colonies in predetermined acidic conditions, temperatures, and bile salt concentrations. The number of growing bacterial colonies was calculated using the total plate count method. Results show that ...
Cardiovascular diseases are the leading cause of death worldwide. The long-term aim of cardiovascular disease therapy is to reduce the mortality rate and decelerate the progression of cardiovascular organ damage. Current therapies focus... more
Cardiovascular diseases are the leading cause of death worldwide. The long-term aim of cardiovascular disease therapy is to reduce the mortality rate and decelerate the progression of cardiovascular organ damage. Current therapies focus on recovering heart function and reducing risk factors such as hyperglycemia and dyslipidemia. However, oxidative stress and inflammation are important causes of further damage to cardiovascular organs. Caesalpinia sappan Linn. (Fabaceae), a flowering tree native to tropical Asia, has antioxidant and anti-inflammatory properties. It is used as a natural dye to color food and beverages and as a traditional treatment for diarrhea, diabetes, and blood stasis. The phytochemical compounds in C. sappan, mainly the homoisoflavonoids brazilin, sappanone A, protosappanin, and hematoxylin, can potentially be used to protect cardiovascular organs. This review aims to provide updates on recent developments in research on C. sappan in relation to treatment of car...
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Objective: In this study, the embryonic rat cardiomyocyte cell line H9C2 was used to investigate the cardiotoxicity effect of sappan wood ethanol extract (SWEE). Methods: Sappan wood was extracted in 96% ethanol and divided into dose... more
Objective: In this study, the embryonic rat cardiomyocyte cell line H9C2 was used to investigate the cardiotoxicity effect of sappan wood ethanol extract (SWEE). Methods: Sappan wood was extracted in 96% ethanol and divided into dose concentrations of 2.5, 5, 10, 50, 100, and 150 μg/ml, with deferiprone used as a control. Cell viability was assessed using the PrestoBlue Cell Viability Reagent, according to manufacturer protocols. Results: Microscopic examination showed that the cell viability of H9C2 was preserved by SWEE treatments at a dose of 10 μg/ml and suggested dose concentrations of 50 μg/ml of SWEE. The percentage of viable cells was greater than 95% with a dose concentration of 10 μg/ml of SWEE, but it was significantly reduced with a dose concentration of 50 μg/ml of SWEE (p<0.05). Conclusion: The optimal dose concentration of SWEE to reach 95% cell viability was 10 μg/ml.
This research was carried out to detect the effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the liver structure of iron overload rat (Rattus norvegicus).The method of experimental used Completely... more
This research was carried out to detect the effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the liver structure of iron overload rat (Rattus norvegicus).The method of experimental used Completely Random Design (CRD in triple repetition. The treatment had been carried out orally. Iron dextran with total dose of 1.5 g kg-1 of body were given to rat on the first, fourth, seventh, ten and thirteenth day. Sappan Wood Extract (Caesalpinia sappan)200 mg kg-1 bw, 400 mg kg-1 bw, Wheat grass extract 100 mg kg-1 bw and Vitamin E 60 mg kg-1 bw were given to rat everyday for 15 days. At the seventeenth, rat were killed and their liver were taken. The observed parameters are morphological abnormality including the colour, the contour, ratio between liver weight and body weight as well as histological destruction. The result showed iron dextran treatment was proved the abnormality on morphological and histological desruction. Futhermore, Sappan Wood Extr...
Most of the case of patient with hemoglobin disorder genes need routine blood transfusion throughout their life. It leads to increase iron accumulation with liver as the main organ for iron storage. Liver is the main organ for metabolic... more
Most of the case of patient with hemoglobin disorder genes need routine blood transfusion throughout their life. It leads to increase iron accumulation with liver as the main organ for iron storage. Liver is the main organ for metabolic process, including triglyceride and cholesterol. However, correlation between metabolism of triglyceride and cholesterol, and iron overload condition is remain uncertain. The purpose of this study was to investigate the effect of iron overload to triglyceride and cholesterol level in the serum of mice. Keywords: iron overload; Triglyceride serum; Cholesterol Serum.
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Untuk mengetahui apakah membran alginat dapat digunakan sebagai produk alternatif tekstil medispembalut luka primer, maka telah dilakukan uji pre klinis untuk mengobati luka pada kulit kelinci albino jantan,sesuai dengan standar yang... more
Untuk mengetahui apakah membran alginat dapat digunakan sebagai produk alternatif tekstil medispembalut luka primer, maka telah dilakukan uji pre klinis untuk mengobati luka pada kulit kelinci albino jantan,sesuai dengan standar yang berlaku (OCDC Guidelines for the testing of Chemicals, 404). Percobaan dilakukanterhadap tiga ekor kelinci albino jantan, yaitu dengan melukai bagian kiri dan kanan punggung kelinci. Bagiankanan punggung kelinci ditempelkan pembalut luka yang kontak langsung dengan luka, sedangkan bagian kirinyatidak (sebagai kontrol). Adapun waktu pengamatannya adalah 1 jam, 24 jam, 48 jam dan 72 jam. Dari hasilpercobaan diketahui bahwa membran dapat mempercepat penyembuhan luka dan tidak menyebabkan iritasi kulit,bahkan setelah tiga hari hampir tidak terlihat adanya goresan bekas luka.
This study was aimedto identify probiotic characteristics and to test the cellulolytic ability of Bacillus megaterium and Bacillus mycoides bacteria for probiotic microbe candidates in fish and shrimp feed. The description of the... more
This study was aimedto identify probiotic characteristics and to test the cellulolytic ability of Bacillus megaterium and Bacillus mycoides bacteria for probiotic microbe candidates in fish and shrimp feed. The description of the cellulolytic and amylolytic abilities of these bacteriawas obtained by non-experimental method and descriptive analysis. Probiotic characteristic identification includes growth curve was obtained through total plate count method, cellular and colony morphology, and cellulase and amylase enzyme activity test using DNS method. Results indicated that the maximum growth of B. megateriumwas observed after six hours at 35.62 x 10 10 (CFU), while B. mycoideswasafter 30 hoursat 42.6 x 10 10(CFU). The macroscopic observation showed that the colony of B. megateriumwas concave and smooth,while B. mycoides was flat, relatively rough, with silken threads around the colony.Both bacteria had milky white color, bacillus shape, Gram positive, and sporous. The activity of ce...
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