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The study aimed to synthesize non-noxious, clean, reliable, and green sulfur nanoparticles (SNPs) from Citrus limon leaves. The synthesized SNPs were used to analyze particle size, zeta potential, UV–visible spectroscopy, SEM, and... more
The study aimed to synthesize non-noxious, clean, reliable, and green sulfur nanoparticles (SNPs) from Citrus limon leaves. The synthesized SNPs were used to analyze particle size, zeta potential, UV–visible spectroscopy, SEM, and ATR-FTIR. The prepared SNPs exhibited a globule size of 55.32 ± 2.15 nm, PDI value of 0.365 ± 0.06, and zeta potential of −12.32 ± 0.23 mV. The presence of SNPs was confirmed by UV–visible spectroscopy in the range of 290 nm. The SEM image showed that the particles were spherical with a size of 40 nm. The ATR-FTIR study showed no interaction, and all the major peaks were preserved in the formulations. An antimicrobial and antifungal study of SNPs was carried out against Gram-positive bacteria (Staph. aureus, Bacillus), Gram-negative bacteria (E. coli and Bordetella), and fungal strains (Candida albicans). The study showed that Citrus limon extract SNPs exhibited better antimicrobial and antifungal activities against Staph. aureus, Bacillus, E. coli, Bordet...
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A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of Levetiracetam in bulk and tablets dosage forms. The separation was achieved on C 18 analytical column (250 mm × 4.6... more
A reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of Levetiracetam in bulk and tablets dosage forms. The separation was achieved on C 18 analytical column (250 mm × 4.6 mm i.d., 5.0 µm) using acetonitrile and distilled water in the ratio 80:20 v/v as mobile phase and at a flow rate of 1.0 mL/min. Detection was carried out using a UV detector at 210nm. The total chromatographic analysis time per sample was about 5.0min with Levetiracetam eluting at retention time of about 3.5min. The method is accurate (99.51% - 100.45%), and the standard curve was linear over the concentration range of 25-150µg/mL with R 2 close to one (0.999) and Y-intercept of 0.022. The limit of detection (LOD) and limit of Quantitation (LOQ) obtained for Levetiracetam were 0.01µg/mL and 0.09µg/mL, respectively. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the determination of Levetira...
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The aim of the study was to isolate and purify high molecular weight acid phosphatase from Rohu fish liver. The purification processes included the enzyme precipitation by ammonium sulphate, chromatographic adsorption by CM-Cellulose, ...
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Research Interests: Biochemistry, Chemistry, Plant Biology, Medicine, Liver, and 12 morePhosphatase, Animals, Fishes, Biological, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Affinity chromatography, Substrate Specificity, Isoenzymes, Molecular weight, Acid Phosphatase, purines, and Pharmacology and pharmaceutical sciences
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of protein was obtained with recovery of nearly 1%. About 222 times purification was achieved. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) resolved two bands of acid phosphatase corresponding to molecular weight... more
of protein was obtained with recovery of nearly 1%. About 222 times purification was achieved. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) resolved two bands of acid phosphatase corresponding to molecular weight of 29 kilo Dalton (kDa) and 18 kDa. The molecular weights of native enzyme determined by gel filtration on calibrated Sephadex G-100 column were found to be 29 kDa and 18 kDa The apparent Km value of 29 kDa isoenzyme with p-nitrophenyl phosphate (pNPP) as substrate was 0.3 mM and Vmax was 1336 nmol.sec -1 .mg -1 of protein. The optimal pH for this enzyme was 5.5 and pH stability was 4-7. It had optimum temperature of 50 o