Prof. Ayman A Diab

In the present study, the effect of methanol extracts of two plant species, Dizygotheca kerchoveana (Maliaceae) and Azadirachta indica (Araliacaea) were tested on the activity of Schistosoma mansoni worms. Efficacy of two plant extracts compared with praziquantel (PZQ) was evaluated in vivo. The results showed that reduction was 90% in case of PZQ treatment compared to 76.91%, 62.64% after treatment with D. kerchoveana and A. indica extracts respectively. PZQ recorded highest significant number of dead ova into oogram pattern (80±1.80) at 5wk PI. But, good results were obtained by of D. kerchoveana and A. indica methanol extracts (65.00±4.05 & 60.60±3.60, respectively) at 3wk PI. Efficacy of the plant extract showed a significant (P<0.01) reduction in ova to 91-96.20% in intestine, 83.36-91.24% in hepatic tissues as compared to PZQ (10-66.50% & 1.14-80.64%, respectively). Reduction in hepatic granulomadiameter at 3wk PI was significantly (P<0.01) reduced in group treated with ...

There is a pressing demand to synthesize polymers that have antibacterial and antifungal properties. The aim of this study was to synthesize a crosslinked hydrophilic terpolymer with acrylamide, acrylonitrile, acrylic acid, acrylamido-2-methylpropane sulphonic acid and ethylene glycol dimethacrylate as a crosslinker. The chemical structure and thermal stability of the prepared cross-linked terpolymers were confirmed by spectroscopic and thermal analyses. Moreover, the swelling experiments were performed to investigate their swelling capacity. Furthermore, the efficiency of the synthesized cross-linked polymer gels was assessed as an antimicrobial agent against Gram-positive, Gram-negative bacteria and fungal strains. The synthesized polymers showed broad inhibition effect, with more antibacterial activity by the AM4 polymer sample containing high percentage of acrylonitrile monomer in the prepared terpolymers (4 mol ratio of acrylic acid: 1 mol ratio of acrylamide: 16 mole ratio of ...

HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and α-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation. Many, such as miR-224, miR-215, miR-143 are correlated with tumor appearance and with the degree of fibrosis in lung, breast and colon cancer. This study was performed to estimate the level of these miRNAs in serum of patients with HCV-associated hepatitis and HCC in relation to grade of hepatitis, stage of fibrosis and differentiation of tumor tissue. In addition, correlations between serological and...

"Findings and recommendations. 1. Scope of cereal seed aid needed in 2002. Assessments of village-level seed aid requirements are urgently needed in cereal-growing areas. Afghani farmers may need about 80,000 MT of wheat seed aid in 2002 of which—by as yet untested assumptions--20,000 MT would be needed in irrigated areas and 60,000 MT in rainfed areas. Rice, maize, and barley seed aid requirements may be on the order of 8000, 7000, and 7000 MT, respectively. Both actual needs and ability to deliver may be much lower. 2. Local versus imported procurement. For rainfed wheat seed aid, an appropriate locally-based strategy to supply 60,000 MT in 2002 may be simply impossible, but perhaps it could take the form of: a) redistribution of existing stocks of seed, including unmilled grain still stored as a single variety with good germination; 15,000 MT; b) replication of rainfed variety seed, including Dayima 94, Ghori 94, and local landraces (subject to vernalization & photoperiod issues) in irrigated fields this spring to produce 35,000 MT; c) import of international variety seed identical or (similar?) to Lalmi 1, Lalmi 2 and Lalmi 3; 10,000 MT. For the hypothetical 20,000 irrigated wheat seed aid, perhaps it would be possible to import for the spring planting 10,000 MT of modern variety seed from Pakistan and elsewhere for distribution only in areas where it has already been found to be well adapted. And for the other 10,000 MT, relying on the existing FAO-led seed production network and the hiring of additional skilled farmers to produce about 10,000 MT of quality seed for redistribution to farmers. 3. Strengthening of the formal seed sector in Afghanistan including the top end of the seed chain. Further programs involving CGIAR centers and National and Foreign University breeding programs would complement the existing seed network created by NGOs and the FAO-Afghanistan Seed Program. Support and capacity building should target both the formal and informal seed sectors and the public and private seed sectors in Afghanistan. Review of crop germplasm collected in Afghanistan over the past 80 years and of exotic crop germplasm tested in Afghanistan over the past 40 years would provide a stronger foundation for the planning of new breeding and crop improvement programs in country. Provision for training of Afghani scientists in the area of seed technology to the graduate degree level would be important to this longer-term undertaking. In the short term, tendering for seed supply at the provincial level coupled with oversight and training of contract farmers may help the more competent contract farmers to upgrade to the status of Small Seed Enterprises."

Biosorption is a bioremediation approach for the removal of harmful dyes from industrial effluents using biological materials. This study investigated Methylene blue (M. blue) and Congo red (C. red) biosorption from model aqueous solutions by two marine macro-algae, Ulva fasciata and Sargassum dentifolium, incorporated within acrylic fiber waste to form composite membranes, Acrylic fiber-U. fasciata (AF-U) and Acrylic fiber-S. dentifolium (AF-S), respectively. The adsorption process was designed to more easily achieve the 3R process, i.e., removal, recovery, and reuse. The process of optimization was implemented through one factor at a time (OFAT) experiments, followed by a factorial design experiment to achieve the highest dye removal efficiency. Furthermore, isotherm and kinetics studies were undertaken to determine the reaction nature. FT-IR and SEM analyses were performed to investigate the properties of the membrane. The AF-U membrane showed a significant dye removal efficiency...

In the past few years, the development of hydrogel properties has led to the emergence of nanocomposite hydrogels that have unique properties that allow them to be used in various different fields and applications such as drug delivery, adsorption soil containing, tissue engineering, wound dressing, and especially antimicrobial applications. Thus, this study was conducted in order to fabricate a novel crosslinked terpolymer nanocomposite hydrogel using the free radical copolymerization method based on the usage of 2-acrylamido-2-methylpropane sulfonic acid (AMPS), acrylamide (AAm), acrylonitrile (AN), and acrylic acid (AA) monomers and iron oxide (Fe3O4) magnetic nanoparticles and using benzoyl peroxide as an initiator and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. The structure of the synthesized composite was confirmed using Fourier transform infrared (FTIR) spectroscopy and x-ray powder diffraction (XRD) measurements. Furthermore, the surface morphology and the magn...

The main objective of this investigation was to assess the genetic diversity among five Egyptian bread wheat genotypes (Misr1 and Sids 13 cutlivars and the promising lines No.1, No.2 and No.11 ) using SDS-PAGE and RAPD markers. The total number of SDS- bands was seven. Six SDS- bands were monomorphic while the other was polymorphic. Line-1 was characterized by the presence of band -3 with a molecular weight of 41.56 kDa. RAPD analysis showed that the number of polymorphic amplicons was 66 out of a total of 93 amplicons, thus revealing a level of 70.97 % polymorphism. The highest genetic similarity revealed by RAPD analysis (93.1%) was between Misr1 and Line 2 genotype. While, the lowest similarity (85.2 %) was between Line 1 and Line 2. The dendrogram separated Line1 from all the other genotypes. The four genotypes constituted a subcluster divided into two groups, one group composed of Misr 1 and Line 2, while the second group comprised Sids 13 and Line 11.

Drought is a major planetary stress problem that places greater limits on cereal productivity. Crop species belonging to the tribe Triticale represent the main foodstuff sources for humans. Because durum wheat (Triticum turgidum L.) shows better adaptation to semi-arid climates than bread wheat, its genome are likely to contain a pool of genes that are expressed for wide environmental adaptability. To investigate and understand the genetic response of drought tolerance in durum wheat, differentially expressed sequence tags (dESTs) were detected, characterized and cloned from durum wheat under different regimes of drought stress and the functions of some of these dESTs were determined. In this study, genes involved in drought stress at early stages of growth were profiled by a differential display technique from Egyptian durum wheat (Sohag 3). One, three, six, and 10 hours of drought stress were applied to produce 15 expressed cDNA fragments, which highlighted the genes that were exp...

L-Arginase, hydrolyzing L-arginine to L-ornithine and urea, is a powerful anticancer, L-arginine-depleting agent, against argininosuccinate synthase expressing tumors. Otherwise, the higher antigenicity and lower thermal stability of this enzyme was the main biochemical hurdles. Since, the intrinsic thermal stability of enzymes follow the physiological temperature of their producer, thus, characterization of L-arginase from thermotolerant Penicillium chrysogenum was the objective of this study. L-Arginase (Arg) was purified to its homogeneity from P. chrysogenum by 10.1-fold, with 37.0 kDa under denaturing PAGE, optimum reaction at 50 °C, pH stability (6.8-7.9), with highest molar ratio of constitutional arginine, glutamic acid, lysine and aspartic acid. The purified enzyme was PEGylated and immobilized on chitosan, with 41.9 and 22.1 % yield of immobilization. At 40 °C, the T1/2 value of free-Arg, PEG-Arg and Chit-Arg was 10.4, 15.6, 20.5 h, respectively. The free-Arg and Chit-Arg have a higher affinity to L-arginine (K m 4.8 mM), while, PEG-Arg affinity was decreased by about 3 fold (K m 15.2 mM). The inhibitory constants to the free and PEG-Arg were relatively similar towards HA and PPG. The IC50 for the free enzyme against HEPG-2 and A549 tumor cells was 0.136 and 0.165 U/ml, comparing to 0.232 and 0.496 U/ml for PEG-Arg, respectively. The in vivo T1/2 to the free Arg and PEG-Arg was 16.4 and 20.4 h, respectively as holo-enzyme. The residual L-arginine level upon using free Arg was 156.9 and 144.5 µM, after 6 and 8 h, respectively, regarding to initials at 253.6 µM, while for Peg-Arg the level of L-arginine was nil till 7 h of initial dosing. The titer of IgG was induced by 10-15 % in response to free-Arg after 28 days comparing to IgG titer for PEG-Arg.

Drought limits cereal yields in several regions of the world and plant water status plays an important role in tolerance to drought. To investigate and understand the genetic and physiological basis of drought tolerance in barley, differentially expressed sequence tags (dESTs) and candidate genes for the drought response were mapped in a population of 167 F8 recombinant inbred lines derived from a cross between "Tadmor" (drought tolerant) and "Er/Apm" (adapted only to specific dry environments). One hundred sequenced probes from two cDNA libraries previously constructed from drought-stressed barley (Hordeum vulgare L., var. Tokak) plants and 12 candidate genes were surveyed for polymorphism, and 33 loci were added to a previously published map. Composite interval mapping was used to identify quantitative trait loci (QTL) associated with drought tolerance including leaf relative water content, leaf osmotic potential, osmotic potential at full turgor, water-soluble carbohydrate concentration, osmotic adjustment, and carbon isotope discrimination. A total of 68 QTLs with a limit of detection score > or =2.5 were detected for the traits evaluated under two water treatments and the two traits calculated from both treatments. The number of QTLs identified for each trait varied from one to 12, indicating that the genome contains multiple genes affecting different traits. Two candidate genes and ten differentially expressed sequences were associated with QTLs for drought tolerance traits.

Carboplatin is a synthetic antineoplastic agent used for cancer treatment and is considered to be the analogous of cisplatin. Nigella sativa oil is a herbaceous plant, it has been used for thousands of years for culinary and medical purposes. This study aimed to evaluate the effect of carboplatin and Nigella sativa oil alone and in combination together on human breast cancer cells (MCF-7) in vitro and Ehrlich ascites tumor bearing female mice (in vivo). The in vitro experiment on MCF-7 cells illustrated that IC50 of carboplatin was 11.8 μg/ml, also IC50 of Nigella sativa oil was 39 μg/ml on MCF-7 cells. In addition, IC50 of the combination between carboplatin and Nigella sativa oil was found to be 3.78 and 40 μg/ml, respectively. The in vivo experiment illustrated that carboplatin (10mg/kg) increased the enzyme activity of aspartate amino transferase (GOT) and aniline amino transferase (GPT)by 56.52% and 51.14%, respectively as compared to both healthy control (nontumor transplanted mice) and negative control. Also, the activity of GOT and GPT was increased by 14.75% and 19.84%, respectively as compared to healthy control under the effect of Nigella sativa oil (12ml/kg). While the activity of GOT and GPT was decreased as compared to negative control. The combination of carboplatin and Nigella sativa oil appeared to increase the enzyme activity of GOT and GPT by 62.41 and 49.39%, respectively as compared to both healthy control and negative control. Also, carboplatin induced DNA damage of liver tissue was performed by agarose gel electrophoresis and comet assay, while Nigella sativa oil showed intact DNA without any damage. The combination of carboplatin and Nigella sativa oil appeared to decrease the DNA damage as compared to carboplatin alone.

Abstract: Polyamines (PAs) are polybasic aliphatic amines that are ubiquitous in prokaryotic and eukaryotic organisms. It is believed that PAs play important roles in cell division, embryogenesis, floral and fruit development, root formation and defense against environmental stress including biotic and abiotic stresses. It has been well documented that PAs are closely associated with resistance of plants to drought stress. S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme in polyamine biosynthesis. A fulllength cDNA for SAMDC gene from durum wheat, designated TtSAMDC was isolated by the RT-PCR approach and was found to be different from another SAMDC genes released in GenBank. TtSAMDC encoded a peptide of 386 amino acids. The main ORFs of TtSAMDC encoded protein with predicted molecular masses of 42.4 KDa (pI 4.79). Comparison of the deduced polypeptide of TtSAMDC with SAMDC proteins from other plant species revealed several homologous regions, in particular the conserved p...

Banana is one of the most important fruit crop worldwide, as its production reaches approximately 70 million tones per year. By tissue culture, triploid and seedless bananas can now be produced in a high quantity, in a less time and at any time of the year without being limited to a season. The aim of this study is to substitute the artificial hormones used in the tissue culture such as, cytokinins and auxins by natural materials such as, Pineapple, Coconut milk, Cacao, Coffee, and Charcoal. Five different media composed of these natural materials were prepared in different concentrations, Pineapple (5,10 and 20cm), Coconut milk (5,10 and20cm,), Cacao (1,2.5 and 5 g), Coffee (1,2.5 and 5 g) and Charcoal (1,2.5 and 5 g) to study their effect on the development and browning of the bananas' shoots and roots in vitro, with the aid of various statistical analyses. The results indicated that the pineapple and coconut milk produced the highest number and length of both shoots and roots...

Squash is considered as one of the most important crops in Egypt and worldwide. Zucchini yellow mosaic potyvirus (ZYMV) is considered as one of the most important viruses infects squash. We used the biological, serological and cytopathological studies to identify an Egyptian isolate of ZYMV. Results showed that the Egyptian isolate of ZYMV showed systemic symptoms in the form of severe mosaic and vein banding on Cucurbita pepo cv. Eskandarani under open field conditions. Direct antigen coated (DAC)-enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies was used as a diagnostic tool for detecting ZYMV in the virus-infected C. pepo samples. It showed positive reactions with ZYMV antiserum. Filamentous virus-like particles measuring 750X13 nm was successfully purified from ZYMV-infected squash plants, based on the use of polyethylene glycol and ultracentrifugation. The electron microscope of ultrathin sections of virusinfected leaf tissues revealed the presence of cylind...

Efficient regeneration methods are a priority for the successful application of genetic engineering to vegetatively propagated plants such as grape (Vitis vinifera L.). An efficient in vitro shoot micropropagation protocol leading to the regeneration from shoot tip explants was successfully developed for mass production. Callus was induced from shoot tips and axillary buds on C2D medium supplemented with 4 mg/l 6-benzyladenine (BA). Bud differentiation from callus was possible on C2D medium supplemented with 1 mg/l gibberellic acid and 0.1 mg/l kinetin. Bud initials were elongated by sub-culturing on elongation medium containing C2D salts supplemented with 0.1 mg/l BA and resulting shoots could be mass micropropagated on C2D medium supplemented with 1 mg/l BA. Shoots that were regenerated via organogenesis or micropropagation were rooted on C2D medium supplemented with 1 mg/l indole-3-butyric acid. In this study, regeneration and micropropagation of grape cv. 'Spero' was suc...

Drought stress is one of the most important environmental factors reducing cereal yields. The genetic and physiological bases of drought tolerance in durum wheat was investigated by QTL mapping and by mapping candidate genes derived from differentially expressed genes and previous investigations. A recombinant inbred population derived from a cross between two durum (Triticum turgidum L. var durum) parents Jennah Khetifa and Cham1 that exhibit contrasting traits for drought tolerance was employed. Twelve known genes and 103 differentially expressed sequence tags (dESTs) were surveyed and 55 detected polymorphism between the two parental lines. In total, 162 loci including 6 known genes, 37 dESTs, and an additional 119 markers that were unlinked in the previous map have been merged with 306 previously mapped markers to produce a new map with 468 loci. Single point analysis and composite interval mapping were used to identify the genomic regions controlling traits related to drought s...

... 1996). However, working with an improved linkage map, Diab et al.(2004) found only two significant QTL for OA per se on chromosomes 3H and 5H, but three loci controlling osmotic potential and three QTL for relative water content un-der stress conditions. ...

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Polyamines (PAs) are polybasic aliphatic amines that are ubiquitous in prokaryotic and eukaryotic organisms. It is believed that PAs play important roles in cell division, embryogenesis, floral and fruit development, root formation and defense against environmental stress including biotic and abiotic stresses. It has been well documented that PAs are closely associated with resistance of plants to drought stress. S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme in polyamine biosynthesis. A full- length cDNA for SAMDC gene from durum wheat, designated TtSAMDC was isolated by the RT-PCR approach and was found to be different from another SAMDC genes released in GenBank. TtSAMDC encoded a peptide of 386 amino acids. The main ORFs of TtSAMDC encoded protein with predicted molecular masses of 42.4 KDa (pI 4.79). Comparison of the deduced polypeptide of TtSAMDC with SAMDC proteins from other plant species revealed several homologous regions, in particular the conserved proenzyme...

Based on transgenic cotton lines carrying the bacterial mtlD gene and non-transgenic, conventional cotton variety, a greenhouse experiment was conducted to assess the effect of different levels of salt stress on the amino acid content of both transgenic and non transgenic seeds. The amino acid profile was determined by GC mass-spectrum. Transgenic and non-transgenic cotton seeds were grown to maturity under different concentrations of salt stress after determining the field capacity. Seeds obtained from transgenic and non transgenic plants showed increasing concentration of amino acids with increasing the level of salt stress. However, the transgenic cotton seeds accumulated significant amounts of amino acids compared with non- transgenic seeds. There were some differences in mean content of some individual amino acids between transgenic and non transgenic seeds, with some significant differences at higher level of salt stress. Amongst the amino acids that showed significant differe...

An integrated barley consensus map was constructed and used with durum wheat and rice maps to develop comparative genetic maps for durum wheat, barley and rice. Comparative maps were constructed in three stages, each adding a new layer of information. In the first stage, comparative maps were constructed based on common markers present in durum wheat (A and B genomes) and barley genomes compared to rice maps. In the second stage, the marker sequences were matched according to sequence similarity across species. In the third stage, the sequences of drought candidate genes and differentially expressed sequence tags (dESTs) were compared to Bacterial Artificial Chromosome /Phage Artificial Chromosome sequences of the rice genome. The analysis of stress-related genes and dESTs in durum wheat, barley and rice revealed that the genetic response to drought stress is partially conserved among these species. Comparative maps identified conserved genomic regions that are associated with quant...

The rhizospheres of Viccia faba, Zea mays and Triticum aestivum were used for the removal of PAH compounds from an oil-polluted desert soil. The results show that, as a total,PAH compounds were reduced by 84.6%, 69.7% and 64.0% in the rhizosphere of Viccia faba , Zea mays and Triticum aestivum respectively. This is in contrast to 51.8% reduction value in the non-rhizosphere soil. Results of GC analysis for the detection of the loss (%) of the 16 PAH individuals show that the roots of the legume plant Viccia faba were able to remove 1016.3 (81.2%) out of 1251.7 mg/kg soil of the carcinogenic PAH compounds. This in contrast to 65.6% and 52.1% reduction values in the rhizosphere of the monocot plants Zea mays and Triticum aestivum respectively. From the non- rhizosphere soil only 29.9% of the carcinogenic PAHs were removed. A particular notable distinction between the rhizosphere of Viccia faba and of the other two plants is the greater efficiency of Viccia faba roots to degrade the ca...

The present investigation studied the effect of amino acids (Glutamine, Spermidine and Asparagine) with different concentration (50, 250.500 mg/l) used as precursors to produce secondary metabolites (steroids) and growth development during different stages (callus, embryoids and shooting) of date palm (Malakaby cv.). In Embryogenic callus stage, callus volume was the highest (4.00) when treated with any of the three amino acids, 50 mg/l of Glutamine or Asparagine showed no effect compared to the control giving the lowest callus volume (3.00). Total steroids in callus tissues clearly showed that using Glutamine 250 mg/l in medium gave the highest steroid content 0.662 mg/g and percentage (336% of control), while the lowest (0.111mg/g) was found with Asparagine (500mg/l) and 56.35% of control. Glutamine at 250 mg/l resulted in the highest weight of embryos (2.100 gm). As well as, 500 mg/l Spermidine seemed to be the best amino acid used in order to stimulate steroid biosynthesis resul...

Over the past several decades, the development of engineered small particles as targeted and drug delivery systems (TDDS) has received great attention thanks to the possibility to overcome the limitations of classical cancer chemotherapy, including targeting incapability, nonspecific action and, consequently, systemic toxicity. Thus, this research aims at using a novel design of Poly(N-isopropylacrylamide) p(NIPAM)-based microgels to specifically target cancer cells and avoid the healthy ones, which is expected to decrease or eliminate the side effects of chemotherapeutic drugs. Smart NIPAM-based microgels were functionalized with acrylic acid and coupled to folic acid (FA), targeting the folate receptors overexpressed by cancer cells and to the chemotherapeutic drug doxorubicin (Dox). The successful conjugation of FA and Dox was demonstrated by dynamic light scattering (DLS), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), UV-VIS analysis, and diff...

Molecular markers provide a rapid approach to breeding for desired traits. To use them, it is necessary to determine the linkage between quantitative trait loci (QTL) and such markers. This study was conducted to investigate the genetic basis of salinity responses in Egyptian bread wheat (Triticum aestivum L.). In this context, a doubled haploid (DH) population (SGDH) of 139 individuals was produced from the cross between two Egyptian breeding cultivars (Sakha 93, salt tolerant) and (Gemmeza 7, salt susceptible). The DH population was tested under saline hydroponics culture and various plant responses were measured. A molecular genetic map of the SGDH population covering 3645.3 cM, was constructed using Restriction Fragment Length Polymorphism (RFLP), Microsatellite or Simple Sequence Repeats (SSR), and Amplified Fragment Length Polymorphism (AFLP) markers. In total, 325 loci along the 21 wheat chromosomes were mapped. The B genome showed the highest number of mapped markers followe...

Crude oil pollution of water bodies is a worldwide problem that affects water ecosystems and is detrimental to human health and the diversity of living organisms. The objective of this study was to assess the ability of water hyacinth (Eichhornia crassipes (Mart.) Solms) combined with the presence of magnetic nanoparticles capped with natural products based on Myrrh to treat fresh water contaminated by crude petroleum oil. Magnetic nanoparticles based on magnetite capped with Myrrh extracts were prepared, characterized, and used to adsorb heavy components of the crude oil. The hydrophobic hexane and ether Myrrh extracts were isolated and used as capping for magnetite nanoparticles. The chemical structures, morphologies, particle sizes, and magnetic characteristics of the magnetic nanoparticles were investigated. The adsorption efficiencies of the magnetic nanoparticles show a greater efficiency to adsorb more than 95% of the heavy crude oil components. Offsets of Water hyacinth were...

Banana is one of the most important fruit crop worldwide, as its production reaches approximately 70 million tones per year. By tissue culture, triploid and seedless bananas can now be produced in a high quantity, in a less time and at any time of the year without being limited to a season. The aim of this study is to substitute the artificial hormones used in the tissue culture such as, cytokinins and auxins by natural materials such as, Pineapple, Coconut milk, Cacao, Coffee, and Charcoal. Five different media composed of these natural materials were prepared in different concentrations, Pineapple (5,10 and 20cm), Coconut milk (5,10 and20cm,), Cacao (1,2.5 and 5 g), Coffee (1,2.5 and 5 g) and Charcoal (1,2.5 and 5 g) to study their effect on the development and browning of the bananas' shoots and roots in vitro, with the aid of various statistical analyses. The results indicated that the pineapple and coconut milk produced the highest number and length of both shoots and roots, as well as the soluble sugars contents, while the cocoa and coconut milk showed the highest protein content. The charcoal reduced significantly the phenolic content. On the other hand, there was no significant difference between the coffee, cacao. This study has highlighted the benefits and advantages of using natural materials in producing high quality banana.