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    How To Make Calibration Curve

    Uploaded by

    Adibah Adawiyah
    The document provides instructions for creating a calibration curve from concentration and absorbance data: 1) Enter the concentration and absorbance data into a table 2) Select the data and insert a scatter chart 3) Change the chart title to "Calibration Curve" and add axis titles for concentration and absorbance 4) Insert a trendline and include the equation and R2 value to analyze the linearity of the calibration curve.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    How To Make Calibration Curve

    Uploaded by

    Adibah Adawiyah
    70 views3 pages
    The document provides instructions for creating a calibration curve from concentration and absorbance data: 1) Enter the concentration and absorbance data into a table 2) Select the data and insert a scatter chart 3) Change the chart title to "Calibration Curve" and add axis titles for concentration and absorbance 4) Insert a trendline and include the equation and R2 value to analyze the linearity of the calibration curve.

    Original Title

    How to make calibration curve

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    © © All Rights Reserved

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    The document provides instructions for creating a calibration curve from concentration and absorbance data: 1) Enter the concentration and absorbance data into a table 2) Select the data and insert a scatter chart 3) Change the chart title to "Calibration Curve" and add axis titles for concentration and absorbance 4) Insert a trendline and include the equation and R2 value to analyze the linearity of the calibration curve.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    70 views3 pages

    How To Make Calibration Curve

    Uploaded by

    Adibah Adawiyah
    The document provides instructions for creating a calibration curve from concentration and absorbance data: 1) Enter the concentration and absorbance data into a table 2) Select the data and insert a scatter chart 3) Change the chart title to "Calibration Curve" and add axis titles for concentration and absorbance 4) Insert a trendline and include the equation and R2 value to analyze the linearity of the calibration curve.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 3

    1 Insert data for x and y axis in a table

    Concentration
    A250 (y)
    ug/ml (x)
    0 0.002
    Do not include
    10 0.168 data for sample as
    20 0.329 you need to
    30 0.508 calculate it later.
    40 0.66
    50 0.846

    2 Highlight all the data in the table > Insert >


    Charts (Scatter)

    Chart Title
    1
    0.8
    0.6
    0.4
    0.2
    0
    0 10 20 30 40 50 60
    3 Change Chart Title by clicking on the "Chart Title" and type the actual title.

    Calibration Curve
    Absorbance (250 nm) 1

    0.8

    0.6

    0.4

    0.2

    0
    0 10 20 30 40 50 60
    Concentration, ug/ml

    4 Insert Axis Title


    Click Chart > Design > Add Chart Element > Axis Title > Primary Horizontal
    & Primary Vertical

    Calibration Curve
    Absorbance (250 nm)

    0.5

    0
    0 10 20 30 40 50 60
    Concentration, ug/ml
    5 Insert Trendline, Equation and R2
    Click chart > Click + on the right > Click Trendline > Click arrow and More
    Options > Scroll down and Click Equation and R2

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