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Research Final 1

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HYBRID FRUITS AND VEGETABLES

Presented to the

Faculty of Senior Highschool Department

Binangonan Catholic College

Binangonan,Rizal

In Partial Fulfillment

for the subject Research in Daily Life 1 and 2

of the strand

Science, Technology, Engineering and Mathematics

CATAHAN, MARIA FRANCIA R.

CEÑIDO, MARC ANTHONY C.

LASTICA, GERALYN MAE F.

URREA, ARMAND JAMES A.


Binangonan Catholic College Research Paper
Binangonan, Rizal Senior High School
SY.2019-2021 Research in Daily Life 1 and 2

CHAPTER 1

THE PROBLEM AND BACKGROUND OF THE STUDY

This chapter contains a discussion of the background of the study,

statement of the problem, objectives of the study, hypothesis, conceptual

framework, scope and limitations, significance of the study and definition of terms

Background of the Study

A Hybrid is the offspring of two plants or animals of different species or

varieties, such as a mule, Hybrid vegetable is created when plant breeders

intentionally cross-pollinate two different varieties of a plant, aiming to produce

an offspring, that contains the best traits of each of the parents. Cross-pollination

is a natural process that occurs within members of the same plant species.

Hybrid fruits are created through the controlled speciation of fruits that creates

new varieties and cross-breeds. Hybrids are grown using plant propagation to

create new cultivars. This may introduce an entirely new type of fruit or improve

the properties of an existing fruit. Fruits are an excellent source of

essential vitamins and minerals, and they are high in fiber. Fruits also provide a

wide range of health-boosting antioxidants, including flavonoids.

In natural populations, hybridization can act in opposition to divergence,

introduce adaptive variation into a population, drive the evolution of stronger

reproductive barriers. Hybridization is purposefully employed in the breeding of

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Binangonan Catholic College Research Paper
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SY.2019-2021 Research in Daily Life 1 and 2

domesticated plants to take advantage of transient hybrid vigor, move desirable

variation among lineages, and generate novel phenotypes. With the advent of

next-generation sequencing and the availability of genomic data sets has come a

tide of interest in hybridization and introgression. This includes the development

of methods for detecting gene flow and a steadily growing set of empirical studies

of natural hybridization

Payseur and Rieseberg (2016) as well as a shift toward thinking of

phylogenies as reticulate webs rather than strictly bifurcating trees. One reason

for this trend is that genomic data are particularly well suited to address the

problem of detecting gene flow. Another is the growing recognition that

hybridization is widespread and may have significant evolutionary consequences,

a long-held belief about plants that is increasingly extended to animals.

The study of hybridization in plants has a rich history. Verne Grant (1981) noted

that much of the historical work on hybridization in plants could be partitioned into

cataloging the frequency of hybridization and exploring the evolutionary

consequences of hybridization. To this day, this research on hybridization still

focuses on these two themes. In plants, scientific identification of hybrids is

thought to have begun in 1716,

The Researchers goal is to draw connections between the conception and

development of ideas in plant hybridization and the recent and future work in

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Binangonan Catholic College Research Paper
Binangonan, Rizal Senior High School
SY.2019-2021 Research in Daily Life 1 and 2

these areas. This update is not meant to be an exhaustive review of the

literature; rather, the researchers hope to present a handful of research areas

that combine rich histories of botanical and evolutionary thought with exciting

recent advancements. In particular, The researchers consider the ways in which

genomic data have changed how the researchers think about hybridization in

plants and highlight areas that the researchers believe are especially accessible

to genomic study. The researchers also recognize that, while genomic data

provide previously inaccessible insight into the evolutionary history of plant

populations, they are most powerful when combined with classical experiments

Statement of the problem

This Study of hybrid fruits and vegetables aims to conduct pilot

hybridization and to determine the possible outcome of this method. It aims to

answer the following questions What is the outcome of Ordinary Plant breeding?.

What are the difference of Hybrid fruits and vegetables in terms of their

characteristics (color, shape, size, appearance, texture and smell)

Objectives of the study

This study of Hybrid Fruits and Vegetables aimed to answer the following:

1.The important role of the Hybridization to improve the economy

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Binangonan Catholic College Research Paper
Binangonan, Rizal Senior High School
SY.2019-2021 Research in Daily Life 1 and 2

2.The Effectiveness of Hybridization to change something to a plant to be much

better than it is actual appearance

3. To identify the variation of the two vegetables and two fruits

Hypothesis

This study will take the form of a null hypothesis. In which there’s no

significant difference between ordinary breeding and hybridization of plant, the

study aims to test the possibility of breeding fruits and vegetables using the

hybridization process to a two different type of plant, there’s a possibility to

having unique characteristics (color, shape, size, appearance, texture and smell)

that ordinary fruits and vegetable cannot.

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Binangonan Catholic College Research Paper
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Conceptual Framework

INPUT PROCESS OUTPUT

1.Gathering of the 1. Applying Cross-


Materials Pollination
 Seeds of lemon 2. Capsule merging
pomegranate 3.Planting
and
Flower from 4. Proper plant care HYBRID
FRUITS
Bottle gourd, silk
Squash 5.Giving the needs of AND
 Plant pots Plants(water, sunlight
 Garden trowel and fertilizer) VEGETABLES
 Loam soil
 Paint brush 6.Analyzation of Data
 Capsule
 Polyethylene 7.Validation of Data
Tape
2. The fruits that will 8.Instrumentation of Data
Use Lemon and
Pomegranate
3. The Vegetables that
will use Bottle gourd
and silk squash

FEEDBACK

Figure 1.

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Binangonan Catholic College Research Paper
Binangonan, Rizal Senior High School
SY.2019-2021 Research in Daily Life 1 and 2

Research Paradigm about the process of hybridization of fruits and


vegetables
This research follows IPO Format (Input, Process, Output) where in the

input, the researchers made a process that was arranged in order to produce a

hybrid fruits and vegetables. The researchers gathered materials that should be

used in the study that was shown in the figure 1, including the fruits (lemon and

pomegranate) and vegetables (bottle gourd, silk squash) that will use to perform

hybridization.

In Process, the researchers will apply cross-pollination in bottle gourd and

silk squash to produce hybridised vegetables and be planted on months or a

year. Then by applying capsule merging, the lemon and pomegranate will

combine inside the capsule to incorporate and plant it on months or a year. To

assure that the plant will be taken care to produce a hybrid fruits and vegetables

with proper water absorption, sunlight and fertilizer. The researchers will analyze

the data by collecting the opinions of the respondents, checking of validity of the

study.

In output, it is the outcome of the study which the researchers figured out

the unique physical appearance of hybrid fruits and vegetables their size, color,

shape texture and smell through observation.

Scope and Limitations

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Binangonan Catholic College Research Paper
Binangonan, Rizal Senior High School
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This study will take months or a year (It will depends how long the plant

will grow), to see the result, The researchers will use pollination process to

combine the traits of two different kind of vegetables(Bottle gourd and Silk

Squash) while type of fruits(Lemon and Pomegranate) two different are in

capsule merging where in the two different seeds will confine inside the capsule

and plant it, Pollination is the process in which pollen is transferred from the male

part of a plant to the female part of a plant. The researchers will base only in the

observation of physical appearance and the characteristics of the hybrid fruits

and vegetables.

Significance of the Study

To the Farmers, this can be great for those working in agriculture as they

can reap greater crops and make more money and to obtain information in

hybridization of fruits and vegetables.

To the Economy needs, this study will help them to gain sufficient

knowledge to have business that has something unique because now a days

when their something new, the people want to try or talk about it. There are so

curious how does it works

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Binangonan Catholic College Research Paper
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To the Future researchers, this study is also substantial to the future

scientist as a head start for extracting new set of ideas in hybridizing two different

types of plants

To the Researchers themselves, the study is important for the researchers

to learn more about the process of hybridization of plants and to help them grow

more knowledge, understanding and wisdom.

Definition of terms

The key terms in the study are given the following operational definitions.

Genomic data- it is an analysis to determine the function of specific genes.

Hybridization- the process of an animal or plant breeding with an individual of

another species or variety.

Novel phenotypes- is a new or different feature of an organism. These traits

come from the different way genes combine and interact with each other

Phylogenies- A similar model or diagram delineating the development of a

cultural feature.

Ordinary plant breeding- Is the purposeful manipulation of plant species in

order to create desired genotypes and phenotypes for specific purposes.

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Cross pollination- pollination of a flower or plant with pollen from another flower

or plant.

CHAPTER 2

REVIEW OF RELATED LITERATURE

This Chapter shows the different kind of literature regarding the foreign

literature of hybridization of fruits and vegetables and ordinary breeding. This first

part of the Review Deals with the hybridization of fruits and vegetables and

ordinary breeding including some historical and latest events of information about

hybridization the importance and its function. The Second part deals with the

purpose of Hybridization and Ordinary Breeding and effectiveness of cross-

pollination are our focus in the variables.

Hybridization

According to The Journal of Xi’an Polytechnic University (2015) It is

pointed out that appropriate hybridization is beneficial to the richness,

development and promotion of culture, and also to advocating the equality,

difference and pluralism of culture under the background of globalization.

According to B.Goulet, F.Roda, and R.Hopkins (N.A) Hybridization has

played an important role in the evolution of many lineages. With the growing

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availability of genomic tools and advancements in genomic analyses, it is

becoming increasingly clear that gene flow between divergent taxa can generate

new phenotypic diversity, allow for adaptation to novel environments, and

contribute to speciation. Hybridization can have immediate phenotypic

consequences through the expression of hybrid vigor. On longer evolutionary

time scales, hybridization can lead to local adaption through the introgression of

novel alleles and transgressive segregation and, in some cases, result in the

formation of new hybrid species. Studying both the abundance and the

evolutionary consequences of hybridization has deep historical roots in plant

biology. Many of the hypotheses concerning how and why hybridization

contributes to biological diversity currently being investigated were first proposed

tens and even hundreds of years ago. In this Update, we discuss how new

advancements in genomic and genetic tools are revolutionizing our ability to

document the occurrence of and investigate the outcomes of hybridization in

plants.

According to The International Journal of Cultural Studies 8 (2), 175-193,

(2005) discussed that Hybridization has become part of an ongoing trend in

cultural production, with both the globalization and localization of the culture

industry. Hybridization, however, is not merely the mixing, blending and

synthesizing of different elements that ultimately forms a culturally faceless

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whole. In the course of hybridization, cultures often generate new forms and

make new connections with one another.

According to Ehrlich and Wilson (1991) and Rieseberg (1995), Natural

hybridization is an important process in plant evolution. It has been estimated

that 30–70 % of all flowering plant species have hybridization events in their

phylogenetic histories Therefore, hybrid zones are interesting models for

studying the evolution of reproductive barriers, the role of selection in maintaining

species differences and how phenotypic traits differ between hybridizing

populations (Abbott et al. 2013; Arnold 2014).

According to Soltis PS, et al. Annu Rev Plant Biol. (2009). The importance

of hybridization in plant speciation and evolution has been debated for decades,

with opposing views of hybridization as either a creative evolutionary force or

evolutionary noise. Hybrid speciation may occur at either the homoploid (i.e.,

between two species of the same ploidy) or the polyploid level, each with its

attendant genetic and evolutionary consequences. Whereas allopolyploidy (i.e.,

resulting from hybridization and genome doubling) has long been recognized as

an important mode of plant speciation, the implications of genome duplication

have typically not been taken into account in most fields of plant biology. Recent

developments in genomics are revolutionizing our views of angiosperm

genomes, demonstrating that perhaps all angiosperms have likely undergone at

least one round of polyploidization and that hybridization has been an important

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force in generating angiosperm species diversity. Hybridization and polyploid

formation continue to generate species diversity, with several new allopolyploids

having originated just within the past century. The origins of polyploid species-

whether via hybridization between species or between genetically differentiated

populations of a single species-and the immediate genetic consequences of

polyploid formation are therefore receiving enthusiastic attention. The time is

therefore right for a review of the role of hybridization in plant speciation.

Ordinary Plant breeding

According to Gepts, Hancock (2006) and Moose, Muhm (2008) Varshney

et al. (2009) Plant breeding is an applied multidisciplinary science. It uses

different knowledge areas to generate, select and fix phenotypes in the

development process for obtaining cultivars that better adjust to producer’s and

consumer’s needs. The main objectives of plant breeding are increasing yield,

nutraceutical quality and commercial value traits. To achieve them, different

alternate alleles found in the wild and native populations of each species could

be used, because they underwent different evolutionary and domestication

processes, following human needs.

According to A.Halluer (2011) Plant breeding is considered one of the

longest ongoing activities undertaken by humans, who select plants more

productive and useful to themselves and the animals for at least 10,000 years

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ago. The evolution of civilizations paralleled the success of plant breeding,

although this has not been recognized by the public. The reason may be lack of

understanding of what plant breeding encompasses. The concept of plant

breeding evolved, depending on the time it was formulated, but without losing the

essence of being art and science of manipulating plants for man. This review

discusses the evolution of the concepts and the methods of plant breeding, here

divided arbitrarily into selection based on phenotypes, breeding values and

genotypes. No matter how big the pool of genetic information in recent years, the

phenotype will continues to be important in the present and future. Key words:

concepts of plant breeding, phenotypes, breeding values, genotypes.

B.Ansaldi,S.Franks,J.Weber (2018), Plant breeding systems can vary

widely among populations, yet few studies have investigated abiotic factors

contributing to variation across a broad geographic range. They investigate

variation in reproductive traits of Triodanis perfoliata (Campanulaceae), a species

that exhibits dimorphic cleistogamy, a condition in which indi- vidual plants have

both closed (selfing: cleistogamous: CL) and open (selfing or outcrossing:

chasmogamous: CH) flowers. Chasmogamous production is theorized to be

more costly because CH flowers have a larger exposed surface area and thus

are more likely to lose more water than CL flowers.. These results support the

hypothesis that variation in the environment drives variation in breeding system

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allocation. Our broad geographic analyses provide a framework for mechanistic

studies of cleistogamy, and employ a novel approach for examining reproductive

traits and environmental variation at large scales. Given that two major

components of our models were temperature and precipitation, our study further

emphasizes the potential for ongoing climate change to alter plant breeding

systems.

Cross-Pollination

According to D. K. Ahrent, C. E. Caviness (1994) Previous experiments

have shown that some cross‐pollination (usually <1%) occurs in soybean

[Glycine max (L.) Merr.] under field conditions. Information is needed on a

broader spectrum of germplasm to determine if genotypes differ significantly for

this trait and should be managed differently to produce genetically pure seed in

small plots bordered by other genotypes, Twelve soybean cultivars differing in

maturity were evaluated during two growing seasons to determine the extent of

cross‐pollination under field conditions where both honey bees (Apis mellifera L.)

and indigenous insect populations were present. Flower color, pubescence color,

and quintafoliolate leaves were used as genetic markers to measure extent of

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cross‐pollination when plants were grown in adjacent rows 102 cm apart. In

maturity group (MG) IV, cultivars did not differ significantly (P > 0.05) for percent

cross‐pollination in 1990, but were different in 1991 and when the 2 yr were

combined. ‘Competitor’ was significantly higher for percent cross‐pollination

(1.22%) than the other cultivars within this MG. Cultivars within MG V and V1

differed significantly in 1990 but were not different in 1991. Based on a 2‐yr

average, cross‐pollination varied from a low of 0.09% for ‘Walters’ to a high of

1.63% for ‘Brim’. Results show that cultivars differ significantly (P > 0.05) in the

extent of cross‐pollination and as much as 2.5% outcrossing may occur in MG IV,

V, and VI cultivars in some environments where adequate pollinators are present

and other conditions are favorable. Based on these results, soybean breeders

should use greater isolation of initial progeny rows and breeder seed of a new

cultivar than has commonly been used in the past because of the increased

potential for outcrossing of some cultivars.

According to Craig et al. (2008), Aheto et al. (2011), Kleppin et al. (2011)

and Bialozyt (2012). For instance, GIS has been used to map the distribution of

GMO crops, to model gene flow between genetically modified and conventional

field or natural environment due to cross-pollination, and to relate these data with

additional environmental data on climate, soil, and agricultural patterns or

conservation areas containing protected species and habitats. Additionally, GIS

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has been be used to support sites selection for environmental monitoring, to

manage coexistence of GM crops with conventional and organic farming, as well

as with nature conservation issues contributing in the For instance, GIS has been

used to map the distribution of GMO crops, to model gene flow between

genetically modified and conventional field or natural environment due to cross-

pollination, and to relate these data with additional environmental data on

climate, soil, and agricultural patterns or conservation areas containing protected

species and habitats Additionally, GIS has been be used to support sites

selection for environmental monitoring, to manage coexistence of GM crops with

conventional and organic farming, as well as with nature conservation issues

contributing in the determination of the isolation distances needed to assess

feasibility of coexistence measures. GMO monitoring has been also

complemented by a Web-based geo-information system.

According to A. Huwadens, G. Pufal, A. Bogehols, M. Klein. The Journal

of Agricultural Science. Issue 5, Vol 152. pp 770-778 (2014) Winter oilseed rape

(Brassica napus) is an important crop for human consumption and biofuel

production and its production is increasing worldwide. It is generally assumed

that cross-pollination by insects increases oilseed rape yield but testing of this

has been restricted to a few rapeseed varieties and produced varying results.

The present study determines whether cross-pollination benefits a number of

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oilseed rape varieties by comparing yield in the presence and absence of insects.

Four rapeseed varieties (Sherlock, Traviata, Treffer and Visby) were used with

ten individuals each in four pollination treatments: (1) supplementary hand-

pollination, (2) open pollination with insects able to access the flowers, (3) wind

pollination and (4) autonomous self-pollination. Across all four varieties, open

and supplementary hand-pollination treatments resulted in higher fruit set,

numbers of seeds per pod and seed yield compared with wind and self-

pollination. The cross-pollination benefits, however, differed among rapeseed

varieties: Treffer and Visby had a higher dependence on open (insects) and

supplementary cross-pollination than Sherlock and Traviata. Across all four

varieties, seed weight compensated for reduced fruit set and was highest when

plants were self-pollinated. The present results highlight the importance of

considering varietal differences in crop pollination research. Information on the

pollination requirements of crop varieties is required by farmers to optimize

management decisions in a world of increasing agropollination deficits.

According to L. Crespel, J. Mouchette (2017) Cross-pollination is the

process of applying pollen from one flower to the pistils of another flower.

Pollination occurs in nature with the help of insects and wind. This process can

also be done by hand to produce offspring with desired traits, such as colour or

pest resistance. To cross-pollinate it is necessary to prepare two different roses

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that are at a similar stage of development. In general, in contrast to diploid roses

which are mostly self-incompatible, tetraploid roses are self-compatible. To

prevent self-pollination of the selected seed parent, the anthers (pollen sacs)

must be removed from the blooms before any pollen is released.This is best

accomplished at sunrise when blooms are in the one-third to one-half open

stage. First, all of the petals are removed. This procedure allows better access to

the centre of the flower. Next, with tweezers or small scissors, the anthers are

removed. If the variety selected as a seed parent will also be used as a pollen

parent with other roses, the anthers may be collected in a cup and placed

uncovered in a dry place where they will mature and release pollen by the next

day. After this operation of emasculation has been performed, the remaining

portion of the bud is covered with a white paper bag to prevent unwanted pollen

from reaching the stigmas.

According to The NBER Conference on Emerging Industries:

Nanotechnology and Nanolndicatros (2003) The emergence of new research

based organizational fields drives both scientific progress and economic growth.

The emergence of fields necessitates a movement of knowledge between

participants within the field, but little is known about the drivers and dynamics of

knowledge diffusion within emerging fields. Research has shown that

crosspollination of knowledge plays an important role in innovative processes.

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Binangonan Catholic College Research Paper
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However, these studies investigated cross-pollination at the team or individual

level or through case-studies of individual technologies while assuming that

cross-pollination occurred between concepts. In this paper we move the unit of

analysis to the level of the individual concept, and investigate how the cross-

pollination of concepts influences concept mobility. The paper, thus, extends the

literature’s consideration of the impact of cross-pollination on innovative

outcomes to investigating how cross-pollination influences knowledge dynamics.

Our setting is the crosspollination of knowledge between nanotechnology and

biotechnology, which yielded the new subfield nanobiotechnology. Drawing on a

large dataset of publications, patents and press releases between 1991 and

2005 we track how 133,128 concepts move from science to technology and

commercialization. We find strong support for the hypothesis that crosspollination

facilitates concept mobility. Scientists who reside in commercial firms generally

assist the mobility of concepts, but hinder the mobility of cross-pollinated

concepts.

SYNTHESIS
The researchers determine that the hybridization is still used in the

present to improve and to change something new to a plant or an individual.

Even though, tens and hundreds of years ago were performed the hybridization,

For now, advanced genomic and genetic tools revolutionizing ability to document

the occurrence and investigate the outcome of hybridization of plants.

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Binangonan Catholic College Research Paper
Binangonan, Rizal Senior High School
SY.2019-2021 Research in Daily Life 1 and 2

The hybridization has been trend in cultural production to generate new

forms and make new connections with one another. The important process of

plant evolution is to examine two different barriers of different species and in

plant speciation. While in ordinary breeding is continue to be used to reproduce

another species and fix phenotypes and increasing yield of quality commercial

value traits by using alternative alleles. Ordinary Plant breeding is one of the

longest ongoing activities undertaken by humans and to have productive plants,

no matter how big the pool genetic information it can vary widely among

populations and employ novel approach for examine reproductive traits and

environmental variation at large scale.

The Cross-pollination is a knowledge plays an important role in innovative

processes. To cross-pollinate it is necessary to prepare two different flower from

a Plant that are at a similar stage of development. Cross-Pollination is effective

for combining the two different traits of plants that can produce another plant that

are more good than the old one.

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CHAPTER 3

RESEARCH METHODOLOGY

This chapter presents the methodology utilized in the study. It includes a

discussion of the research design, locale of the study, Materials, research

instruments, procedures.

Research Approach

The type of this research is qualitative research where the researchers will

base in the respondent’s opinions about hybrid fruits and vegetables. The

approach of this study is case study where in the researchers will observe the

plant until it produce the results of the hybrid plant furthermore, the researchers

will observe the characteristics of the hybrid fruit (Color, Shape, Size, texture and

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smell) and compare it to its original appearance so that the researchers will

differentiate the hybrid fruits and vegetables.

Research Design

This study will use experimental research design to determine the

effectiveness of the hybridization of fruits and vegetables to differentiate the

outcome of hybridization and ordinary breeding. Using of this method, this will

help to know the important role of hybridization to improve the environment.

Materials

NAME PICTURE

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Flower of lemon, pomegranate and

seeds of bottle gourd and silk

squash

(male and female)

- Use to get the traits from

different fruits/ vegetables

Plant Pots

- It is used to plant plants

polyethylene tape

-It is used to sealed the two flowers

Garden Trowel

- A small hand tool to use for

digging, applying, moving a small

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amounts of soil.

Loam Soil

- It is a combination soil,

normally equal parts of clay, silt, and

sand. It is suitable for growing most

plant varieties.

Paint brush

- It is used to transfer the female

pollen to the male pollen

Capsule

-It use to merge the two seeds to

combine their traits

Procedures

PROCEDURE (PLANT 1 Leomegranate)

1. Find a place that is suitable to plant

2. Gather the seed of lemon and pomegranate

3. After that put it inside a capsule and plant it

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4. Water the plant and wait until it grows

PROCEDURE (PLANT 2 Bottle Silky Squash Gourd)

1. Plant the bottle gourd and the silky squash seeds until it grows and have

flower

2. Gather flowers from bottle gourd and silky squash. It should be male and

female flower

3.The flower should be matured enough to perform cross-pollination.

4. The Pollens of male flower will sprinkle to the stigma of female flower

5. The male flower overlie the female flower and sealed it with polyethylene tape

Setting of the study

This study will be conducted at Cequeña’s residence Brgy.Macamot,

Binangonan, Rizal. The researchers selected this location because this place is

good for gardening and have sufficient needs of plants.

Map of Cequeña’s Residence

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Subject of the study

In this experimental study, The researchers will determine the

effectiveness of hybridization of fruits and vegetables by choosing 15

respondents from Binangonan public market. The respondents are mainly

composed of fruits and vegetables vendor. The researchers will ask them what is

more effective to the consumers if the hybrid ones or the original and what is

more preferable in terms of their characteristics (Color, Shape, Texture, Size and

Smell).

Instrumentation

This study will use quasi-experimental in which the research instruments

will use in the study are interview and observation. This chosen instruments will

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be used in collecting of data to test validity and the differences of the research

study being conducted. Interviews will be used to meet face-to-face with vendors

to generate ideas if hybrid fruits and vegetables are effective to sell in their shop.

It is an interaction between the interviewer in which a written questions are stated

to develop responses from the interview.

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