Cell Basics
Cell Basics
Cell Basics
2. CELL THEORY
In 1838, Matthias Schleiden, a German botanist, examined a large number of plants. He
concluded that all plants are made up of different cells which make tissue.
Theodore Schwann (1839), a British Zoologist, studied different types of animal cells and
plant tissue. He conciuded that animal cells have thin outer layer, which is today known as
plasma membrane.Based on his studies of plants, he concluded that presence of cell wall is
aunique character of plant cell. Schwann proposed the hypothesis that the bodies of
animals and plants are composed of cells and products of cells.
Matthias Schleiden and Theodore Schwann jointly proposed cell theory in 1839. Its main features
are as follows
(i) All living organisms consist of cells and their products.
(ii) All cells are structurally andmetabolically similar.
(ii) Cells perform vital activities of an organism.
(iv) Each cell is unit of heredity.
Objections
(1) Bacteria and cyanobacteria do not bear nucleus and membrane bound cell organelles.
(2) Viruses are acellular and do not contain cellular machinery.
(3) RBCs and sieve tube cells live without nucleus.
(4) Protozoans and many thallophytes have a uninucleate differentiated body that cannot be divided
into cells. They are acellular.
(5) This theory did not explain as to how new cells were formed.
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CELL:THE UNIT OF LIFE
Modern cell theory
It is also known as cell doctrine or cell principle.
(1) All living organisms are made up of cells having cytosol, nucleus, organelles and a
membranes. covering
(4) Functions of living organisms are the sum total of the activities of their cells.
(3) Cell can survive independantly but organelles cannot do so.
(4) It is unit of structure, function and heredity.
(5) Life exist in cells.
(6) Growth of an organism is due to increase in size and number of cells.
(7) New cell arises from pre existing cells "Omnis cellula-e cellula". It is called cell lineage
theory.This concept was given by Rudolf Virchow (1855). The final shape to cell theory was
given by Rudolf Virchow.
Nerve cell
Bmnched and long)
Mesophyll cella
(uDnd and oval)
A tnachesd
(ohongated)
3. CELL ORGANISATION:
On the basis of nucleus, two types of cells are present -
14 70S types of Ribosomes are found. 14 Ribosomes are of 80S types. 70S
ribosomes are found in mitochondria and
plastids.
15 Centriole is also absent 15 Centriole is present in animals and lower
plants
16 e.g.Bacteria, Cyanobacteria, Mycoplasma and 16 e.g.Protists, Fungi, Plants and Animals.
PPLO (Pleuro Pneumonia like organisms)
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CELL: THE UNIT OF LIFE
Typical Bacterla
(1-2 um)
PPLO
(about 0.1 um)
Viruses
Fig:- Acetabularia
Atyplcal Eukaryotic cell (0.02-0.2um)
(10-20 um) (Unlcellular green alga)
Fig:- Comparlson of unicellular organisms
with typical eukaryotic cell
Prokaryotic cell-
Cell that bears naked genetic material i.e. nucleus is without envelop is known as prokaryotic cell. This
group is represented by Bacteria, Blue Green Algae (Cyanobacteria), Mycoplasma. They may vary
greatly in shape and size. The four basic shapes of bacteria are
(a) bacillus (rod like)
" (b) coccus (spherical)
(c)vibrio (comma shaped)
(d) spirillum (spiral)
Pilus
Flagelium
-Hook
Basal body
Filament
Ribosome
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CELL: THE UNIT OF LIFE
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CELL: THE UNIT OF LIFE
Rough endoplasmic
reticulum
Lysosome
Smooth
endoplasmic
reticulum
Plasmodesnata Nucleus
Nucdeolus
Golgi
apparatus
(icrotubule
Nuclear
envelope
Plasma
membrane
Vacuole
-Middlelamella
Cell wall
(a)
Peroxisome Mttochondrion
Cytoplasm
Ribosomes
Chloroplast
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CELL: THE UNIT OF LIFE
Golg Microvll1
apparatus
Plasrma
mermbrane
Centriole
Smooth Peroxiome
endoplasmlc
retlcuhm
Lysosome
Nuclear
envelope Ribosomes
Nucleolus
Mitochondrion
Rough
endoplasmic
Nucleus reticuhum
(b)
Cytoplasm
Cell wall
It was first studied by Robert Hooke in cork cells.
It is outer most dead covering around plant cell. That is secreted by
cytoplasm/(Protoplasm).
Cell wall not only gives shape to the cell and protects the cell from mechanical damage and
infection, it also helps in cell-to-cell interaction and provides barrier to undesirable
macromolecules. Algae have cell wall, made of cellulose, galactarns, mannans and minerals
like calcium carbonate.
In plant cell it is usually composed of cellulose, hemicellulose, pectins and proteins but in
bacteria and BGA it is composed of peptidoglycan and DAPA. In fungi it consists of chitin. It is
absent in Animals, Mycoplasma.
Structure of the cellwall
The diameter of cell wallvaries from 0.1-10um.
A
cell wallcontains (a) matrix (b) fibrils (c) depositions
Resorate the concept
ChemicalCompostion of cell wall:
Matrix: Water-60%. Hemicellulose-5-15%. Pectic Substances-2-8%. Lipids-0.5-3.0%. Proteins-1-2%
Microfibrils: Cellulose / fungus cellulose-10-15%.0ther depostions 0.025%. Cellulose isa main
Component of cell wall.
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CELL:THE UNIT OF LIFE
The cell wall is formed of following layers
(1) Middle Lamella
(ii) Primary wall
(ii) Secondary Wal
(iv) Tertiary wall
(i) Middile Lamella:
It is thin amophous cementing layer for joining of two adjacent plant cells.
It is compOsed of pectin as calcium and magnesium pectate (mainly calcium pactate)
It is absent on the outer free surface of cell and plasmodesmata.
Retting of fibres and softening of fruits are due to dissolution of calcium pectate in middle
lamella by pectinase enzyme.
The cell wall and middle lamellae may be traversed by plasmodesrnata which connect the
cytoplasm of neighbouring cells
(ii) Primary wall:
It is elastic, pemeable and thin, single layered outer most wall layer of plant cell.
Cellulose, hemicellukose and pectin contents are roughly in equal amount in primary wall.
Root hairs, parenchymatous cells and meristematic cells are formed of only primary wal.
It is capable of growth. Its growth takes place by intussusception (Addition of materials with
in the existing wall).
Itgenerally diminshes as the cellmatures.
Middie lamella
Primary wal.
Secondary
wall Lumen
S
S,
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CELL: THE UNIT OF LIFE
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Cell Membrane
It is outermost covering of the cell that is elastic, living, pliable, hydrophilic and selective
permeable membrane.
It is found in both prokaryotic and eukaryotic cells.
Structure
The detailed structure of the membrane was studied only after the advent of the electron
microscope in the 1950s.
Cell membranes can be observed in electron microscope. It appears trilaminar or tripartite layer
under electron microscope.
Meanwhile,chemical studies on the cell membrane, especially in human red blood cells (RBCs),
enabled the scientists to deduce the possible structure of plasma membrane.
Chemically a ceil membrane contains proteins, Lipids, carbohydrates, water. DNA, RNA are
absent.
The ratio of protein and lipid in plasma membrane varies e.g. in human beings, the
membrane of the erythrocyte has approximately 52 per cent protein and 40 per cent lipids.
Proteins are globular in nature including structural, enzymatic, carrier, permease and receptor
proteins.
Lipids are usually phospholipids (arranged in bilayer) that are amphiatic I amphipathic
containing polar hydrophilic heads (outside) and nonpolar hydrophobic tails (inside).
Cholesterol provides regidity and stability to the cell membrane.
Carbohydrates of cell membranes are small unbranched or branched chains of oligosacch arides.
They combine with both lipids and protein molecules on outer surface of the membrane and form
glycolipids and glycoproteins respectively.
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CELL: THE UNIT OF LIFE
Glycoprotein
Polar end
(Hydrophilic)
Non-Polar end
(Hydrophobic)
Lipid
Bilayer.
Protein
Fig:- Unitmembrane model of Robertson
This model is unable to explain the selective permeability and elasticity of plasma membrane.
(ii) Fluid mosaic model:
It was proposed by Singer and Nicolson (1972).
It is most recognized model for plasma membrane.
According to this, the quasi-fluid nature of lipid enables lateral movement of proteins within
the overall bilayer.
This ability to move within the membrane is measured as its fluidity.
They stated that plasmamembrane contains lipid bilayer in which protein are found on both outer
and inner side to form mosaic pattern.
Thus they described it as protein icebergs in sea of lipids.
The fluid nature of the membrane is also important from the point of view of functions like
cell growth, formation of intercellular junctions, secretion, endocytosis,cell division etc.
Dependingon ease of extraction, proteins of membrane are of two types.
(a) Externalor extrinsic proteins:
It is peripheral protein (30% of total protein).
They lie on the surface of the membrane.
It can be easily removed e.g. Spectrin in RBC.
(b) Integral or intrinsic proteins:
It is about 70% of total protein.
They are buried partially or totally in plasma membrane.
These can not be separated easily. e.g. Cytochrome oxidase, Porin Proteins.
They may function as carriers, permeases, enzymes, receptors.
Some large globular intrinsic proteins pass as a helix into the lipid bilayer from outside to inside
to form tunnel proteins or transmembrane proteins,
The transmembrane proteins act as channels for passage of water soluble materials and water.
The plasma membrane is asymmetric due to oligosaccharides which form glycolipids and
glycoprotein alongwith lipids and proteins respectively.
Both glycolipids and glycoproteins form glycocalyx.
Oligosaccharide part in glycocalyx acts as recognition centre, site for attachment and provides
antigen specificity to cell membranes, blood grouping, immune response and matching of tissues in
transplantation of organs.
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CELL: THE UNIT OF LIFE
Sugar Peripheral
Protetn
Phospholiptd
bilayer
Glycoprotein
Gycolipid
Cholesterol
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(D) Active transport: Some ions or molecules are
transported across the membrane against
their concentration gradient, i.e. from lower to the bigher
concentration. Such a transport
IS an energy dependent process, in which ATP is utilised and is called active transport,
e.g., Na+/K+ Pump.
(l) Bulk transport: It take place by two
methods.
(0) Endocytosis: The inward transport of material by means of carrier vesicles is called
endocytosis. It includes two types.
(a) Pinocytosis or Potocytosis (Cell drinking): Intake of fluid substances by plasmalemma
in the form of vesicles (Pinosome) is called
pinocytosis.
(b) Phagocytosis (Cell eating) : Intake of solid food substances by plasmalemma in the form
of vesicles (Phagosome) is called phagocytosis.
(ü) Exocytosis (Cell vomitting or emiocytosis): It is reverse of endocytosis in which waste
materialsare removed from the cell. It involves reverse pinocytosis.
Cytoplasm
It lies between the nucleus and cell membrane.
In both prokaryotic and eukaryotic cells, a semi fluid matrix called as cytoplasm occupies the
volume of the cell.
Cytoplasm is the main arena of cellular activities in both plant cells and animal cells.
Various chemical reactions occur in cytoplasm to keep the cell in living state.
It includes two parts.
Cytosol / Hyaloplasm / Ground plasm:
Liquid part of cytoplasm except cell organelles
It can exist in sol and gel state called plasmasol and plasma gel.
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CELL: THE UNIT OF LIFE
streaming or cyclosis occur in eukarvotic
Cytoplasmic streaming: It is also called as protoplasmic
cells.
Function:
chloroplast in relation to light intensity.
(C) Helpin movement of organelle such as
food vacuole in Amoeba.
(d) Distribution of various substances and
repair of membrane and in heat distritbution.
(e) Fommation of pseudopodia in Amoeba and in
Trophoplasm: It involves cellorganelles and cell inclusions.
(0) Cell nclusions: bodies. They are of three types
They are non-living substances also known as ergastic
and aleuron grains.
(A) Reserve food: It includes starch, glycogen, fat droplets
(i) Starch grains
(ii) Giycogen granules: Animal cells
(iii) Fat droplets: Animal and Plant cells
(iv) Aleurone grains
(B) Excretory or secretory products:
Mucus in several animal cells, essential oils, alkaloids, resins, gums, tanins, latex etc.
(C) Mineral matter:
Silica found in epidermal cells of grasses.
Calcium carbonate crystals (cystolith) found in epidermal cells of momordica, hypodermal leaf
cells of Banyan.
Calcium oxalate occurs in the form of powdery mass (crystal sand) in atropa, star shaped
sphaerophide in Colocasia, Begonia, Chenopodium prismatic crystals in dry scales of Onion,
needie shaped raphides in lemna, Eichhornia.
(I1) Cellorganelles
Plastids:
They are found in all plant cells and in Euglenoids.
These are easily observed under the microscope as they are large.
These are double membrane bound, DNA containing largest organelles in plant cells.
Origin: All types of plastids have common origin from proplastids (sac like non-lamellar
structures).
They bear some specific pigments, thus imparting specific colours to the plants.
Based on type of pigments, theyare of three types.
(i) Leucoplasts (ii) Chromoplasts (iii)Chloroplasts
i. Leucoplasts: Colourless, largest plastids. They are classified on the basis of stored material
(1) Amyloplasts: They store carbohydrates in the form of starch e.g. Potato.
(2) Aleuroplasts or Proteinoplasts: They store proteins.
(3) Elaioplasts: They store oil or fats.
ii. Chromoplasts: They are coloured plastids those have fat soluble carotenoids like carOtene
Xanthophyll andothers are present. e.g. carotene in carrot, lycopene in tomato and chilies.
This gives the partof the planta yellow, orange or red colour.