Toksoplasmosis merupakan penyakit zoonosis yang disebabkan oleh parasit Toxoplasma gondii yang berdistribusi luas di seluruh dunia. Penyakit ini, selain mengkhawatirkan manusia juga dapat berdampak pada ekonomi peternakan karena... more
Toksoplasmosis merupakan penyakit zoonosis yang disebabkan oleh parasit Toxoplasma gondii yang berdistribusi luas di seluruh dunia. Penyakit ini, selain mengkhawatirkan manusia juga dapat berdampak pada ekonomi peternakan karena menyebabkan penurunan produksi, gangguan pertumbuhan dan fertilitas termasuk aborsi, prematur, dan lahir mati. Prevalensi toxoplasmosis di Indonesia sangat tinggi, pada manusia sekitar 40–85%, dan pada hewan berkisar antara 5–80%. Jahe merah dan kunyit merupakan tanaman zingiberaceae yang mengandung antioksidan tinggi, yang diketahui memiliki aktivitas menghambat proses inflamasi dan berpotensi sebagai anti-toxoplasma. Namun, sampai saat ini belum diketahui mekanisme kerja senyawa aktif kunyit dan jahe merah dalam berikatan dengan reseptor protein T. gondii sebagai target pada terapi toxoplasmosis. Dilakukan uji aktivitas senyawa yang terkandung dalam jahe merah dan kunyit sebagai anti-toxoplasma terhadap protein GRA1 T. gondii dengan molecular docking secar...
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Wabah Surra telah terjadi di seluruh Pulau Sumba Indonesia pada tahun 2010 hingga tahun 2012 yang menyebabkan kematian sebanyak 1.159 kuda, 600 kerbau dan sapi. Teknik elektroforesis dengan gel akrilamid yang mengandung sodium dodecyl... more
Wabah Surra telah terjadi di seluruh Pulau Sumba Indonesia pada tahun 2010 hingga tahun 2012 yang menyebabkan kematian sebanyak 1.159 kuda, 600 kerbau dan sapi. Teknik elektroforesis dengan gel akrilamid yang mengandung sodium dodecyl phosphate (SDS PAGE) merupakan metode yang relatif mudah dan murah untuk mengidentifikasi konsentrasi protein yang sangat rendah dalam sampel yang sedikit. Pada elektroforesis, struktur protein primer diperlukan untuk migrasi protein pada gel poliakrilamid. Penambahan β-merkaptoetanol dapat memutuskan ikatan disulfida pada protein multimer menjadi monomer dan dapat juga dilakukan pemanasan protein untuk mendenaturasi protein menjadi struktur primer. Penelitian ini bertujuan untuk melihat profil protein Trypanosoma evansi isolat S371 dengan elektroforesis menggunakan β‐merkaptoetanol dan tanpa β‐merkaptoetanol serta efek pemanasan proteinnya. T. evansi isolat S371 tahan terhadap proses pemanasan dan adanya perbedaan profil protein setelah mengalami redu...
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Surra outbreak in 2012 has led to more than 1,700 animals have died in the province of East Nusa Tenggara (NTT) Indonesia. Surra case sporadically continues throughout the year in various areas, especially Kalimantan, Banten as well as... more
Surra outbreak in 2012 has led to more than 1,700 animals have died in the province of East Nusa Tenggara (NTT) Indonesia. Surra case sporadically continues throughout the year in various areas, especially Kalimantan, Banten as well as other areas. Some reports reveal differences in protein profiles among multiple isolates of T. evansi. Therefore the purpose of this research were to find out the protein profile of each isolate T. evansi in Indonesia and the possible biological differences among them. Eleven isolates originating from the province of East Nusa Tenggara, South Kalimantan and Central Kalimantan, Banten, Lampung and Bengkulu has been isolated and purified Using DEAE. Trypanosoma isolate were frezeethawing repeatedly to obtain soluble protein. Furthermore, soluble protein is treated with heating or without heating and then each was run on SDS PAGE with Coomassie Blue staining. The protein profiles of all isolates were compared each other. The results showed that eleven is...
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Background and Aim: Bovine eimeriosis is a disease caused by apicomplexan parasites of the genus Eimeria. It is one of the most important and widespread bovine illnesses in the world. Some of the identified species of bovine eimeriosis... more
Background and Aim: Bovine eimeriosis is a disease caused by apicomplexan parasites of the genus Eimeria. It is one of the most important and widespread bovine illnesses in the world. Some of the identified species of bovine eimeriosis have morphologically similar oocysts that are difficult to differentiate. For the identification of particular Eimeria spp., diagnostic laboratories are increasingly turning to DNA-based technology. This study aims to develop a multiplex polymerase chain reaction (mPCR) technique based on the internal transcribed spacer-1 (ITS-1) gene for the simultaneous identification of pathogenic Eimeria spp. in cattle from Sulawesi Island, Indonesia.
Materials and Methods: Genomic DNA was extracted by the DNAzol reagent from the purified Eimeria oocysts. Species-specific primers targeting the ITS-1 region were used to amplify the distinct Eimeria spp.
Results: Using PCR ITS-1, this study showed that 36 of 120 fecal samples (30%) were infected by Eimeria spp. The multiplex PCR assay allowed for the simultaneous identification of six major Eimeria spp. in a single-tube reaction. The proportion of mixed Eimeria spp. infections was 100% (36/36). The maximum number of Eimeria spp. was five, and the minimum number was two.
Conclusion: Identification of six pathogenic Eimeria spp. in cattle was successfully carried out by nested multiplex PCR using ITS-1 gene. In the future, a procedure to detect pathogenic Eimeria spp. in one tube reaction will offer economical and save diagnostic time.
Materials and Methods: Genomic DNA was extracted by the DNAzol reagent from the purified Eimeria oocysts. Species-specific primers targeting the ITS-1 region were used to amplify the distinct Eimeria spp.
Results: Using PCR ITS-1, this study showed that 36 of 120 fecal samples (30%) were infected by Eimeria spp. The multiplex PCR assay allowed for the simultaneous identification of six major Eimeria spp. in a single-tube reaction. The proportion of mixed Eimeria spp. infections was 100% (36/36). The maximum number of Eimeria spp. was five, and the minimum number was two.
Conclusion: Identification of six pathogenic Eimeria spp. in cattle was successfully carried out by nested multiplex PCR using ITS-1 gene. In the future, a procedure to detect pathogenic Eimeria spp. in one tube reaction will offer economical and save diagnostic time.
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Background and Aim: Toxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii. The... more
Background and Aim: Toxoplasma gondii is a unicellular coccidian parasite distributed globally and is an important zoonotic pathogen. Approximately 30% of the human population worldwide is chronically infected with T. gondii. The pathogenicity of this species depends on the type originating from the clonal population. Techniques for more accurately determining the type of T. gondii have recently been developed using genetic markers. Specifically, T. gondii has been typed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). This study aimed to identify sets of PCR-RFLP markers that have high power to discriminate genotyping of T. gondii and are easy to use and are easy to use. The objective of this study was to characterize virulent strain isolates of T. gondii by PCR-RFLP using 10 markers with DdeI. Materials and Methods: T. gondii tachyzoites (RH virulent strain) were derived from culture cells at the Indonesian Research Center for Veterinary Science...
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Background and Aim: Eimeria spp. are gastrointestinal protozoans that affect animal productivity, thereby causing symptoms that range from bloody diarrhea to death. These symptoms cause economic losses to farmers. The distribution of... more
Background and Aim: Eimeria spp. are gastrointestinal protozoans that affect animal productivity, thereby causing symptoms that range from bloody diarrhea to death. These symptoms cause economic losses to farmers. The distribution of Eimeria spp. in cattle has, therefore, been reported to have spread widely, especially in the tropics and subtropics. Indonesia is a tropical country at high risk of Eimeria infections. This study aimed to identify the prevalence and risk factors related to the levels of eimeriosis in beef cattle originating from different geographic areas in Indonesia. Materials and Methods: Here, 817 fecal samples were collected from beef cattle in Indonesia, including 282 calves, 535 adults, 530 males, and 287 females. In addition, 156 semi-intensively and 661 intensively managed cattle were randomly collected. Then, fecal samples were analyzed by parasitology examinations. Results: Screening examination using the sugar flotation modification method showed that Eimer...
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Diagnose technique for detection Tryapanosoma evansi have been developed for many years, including Polymerase Chain Reaction (PCR). One of fundamental factors on this technique is primer desain which possess high sensitivity and... more
Diagnose technique for detection Tryapanosoma evansi have been developed for many years, including Polymerase Chain Reaction (PCR). One of fundamental factors on this technique is primer desain which possess high sensitivity and specificity. The aim of the current study was to examine four primer sets for detection of T. evansi on mice. They were ITS 1, ESAG 6/7, RoTat 1.2 VSG, TBR1/2 and TR3/TR4. Balitvet Culture Collection (BCC) – Bangkalan isolate of T. evansi was was injected into two male mice DDY strain. Three days after infection, blood mice was collected and stored at -20oC. In addition, some blood was dropped on the filter paper and kept at 4oC. Another mice was killed and some tissues (brain, liver, lung, heart, spleen and kidney) were removed from mice and preserved into 80% Ethanol and then they were stored -20oC. All samples were amplified using five primer sets and repeated twice. The results demonstrated that all primers (ITS 1, ESAG 6/7, RoTat 1.2 VSG, TBR1/2 and TR3...
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Leishmaniasis is a zoonosis, neglected and poorly reported disease, caused by the protozoan Leishmania spp, genus Leishmania, transmitted through several vector species including the sand fly (gnat) genus Phlebotomus. This disease has... more
Leishmaniasis is a zoonosis, neglected and poorly reported disease, caused by the protozoan Leishmania spp, genus Leishmania, transmitted through several vector species including the sand fly (gnat) genus Phlebotomus. This disease has complex ecology and epidemiology. This paper presents about Leishmaniasis disease from epidemiological aspects and risk factors; parasites, vectors and reservoirs; diseases transmission and control. Leishmania spp. is an obligate intramacrophage protozoan, characterized by its kinetoplast, a unique form of mitochondrial DNA. Clinical manifestations of Leishmaniasis depend on interactions between the characteristics of Leishmania species and the human host immune response, resulting in a diseases spectrum ranging from local lesions on the skin to diffuse involvement of the reticuloendothelial system. There are four forms of Leishmaniasis in humans with various clinical manifestations, namely visceral Leishmaniasis (VL), also known as kala-azar (KA), Cut...
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Coccidiosis is a parasitic disease caused by the protozoan of the order Coccidia, the family Eimeriidae of the genus Eimeria which breeds rapidly in the digestive tract and is the most difficult disease to be controlled on cattle farms.... more
Coccidiosis is a parasitic disease caused by the protozoan of the order Coccidia, the family Eimeriidae of the genus Eimeria which breeds rapidly in the digestive tract and is the most difficult disease to be controlled on cattle farms. Eimeria spp. in cattle can cause high economic losses and increase susceptibility against infectious diseases. Therefore, coccidiosis in cattle needs attention from the government. This paper reviews a number of diagnostic methods that can be used in the detection of Eimeria spp. in cattle based on the goals and objectives of the examination. The coccidiosis cases often do not show any clinical symptoms but can cause sudden death in livestock. The diagnostic method that still used at present is based on its morphology that should not be used in identifying Eimeria species because the morphological characteristics Eimeria spp. have similar shape and size structures between species (resembling morphology). An appropriate diagnostic method for Eimeria i...