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    Syed Shaukat

    Summary. Aqueous extracts of the four weed species Conyza canadensis, Blurnea obliqua, Amararrthus viridis and Eclipta prostrata caused substantial mortality of the plant-parasitic nematodes Xiphinema americanum, Tylenchulus semipenetrans... more
    Summary. Aqueous extracts of the four weed species Conyza canadensis, Blurnea obliqua, Amararrthus viridis and Eclipta prostrata caused substantial mortality of the plant-parasitic nematodes Xiphinema americanum, Tylenchulus semipenetrans and Meloidogyne ...
    The effects of 10 strains of Fusarium solani on Meloidogyne javanica were tested in vitro and in controlled conditions. Culture filtrates of the strains varied with respect to parasitism on eggs and females of M. javanica and nematicidal... more
    The effects of 10 strains of Fusarium solani on Meloidogyne javanica were tested in vitro and in controlled conditions. Culture filtrates of the strains varied with respect to parasitism on eggs and females of M. javanica and nematicidal activity in terms of juvenile mortality. Mortality in boiled culture filtrates was slightly lower than that caused by un-boiled filtrates, but substantial nematicidal activity was retained, pointing to the relative thermostability of the active principle(s) involved. Aqueous and ethyl acetate extracts of F. solani produced higher nematicidal activity than a hexane extract indicating that the active compound(s) were polar in nature. Conidial suspensions of F. solani strains Fs5, Fs9 and Fs10 used as soil drench significantly reduced nematode populations in soil and root-knot disease severity, resulting in enhanced growth of tomato plants. There was no significant difference among F. solani strains on shoot fresh weight. Strain Fs5 was frequently reis...
      Nematodes associated with Mentha spicata L. and Mentha longifolia L. were investigated in Balochistan in various localities so as to assess the variation in density of different nematode species found in the rhizosphere. Ten species of... more
      Nematodes associated with Mentha spicata L. and Mentha longifolia L. were investigated in Balochistan in various localities so as to assess the variation in density of different nematode species found in the rhizosphere. Ten species of nematodes were found associated with Mentha spicata while four species were found in the rhizosphere of M. longifolia. Significant differences in nematode density were recorded (p at the most p < 0.01). The dominant nematodes associated with Mentha spicata were Pratylenchus projectus and Merlinius khuzdarensis while those associated with Menthe longifolia were Xiphinema americanum and Pratylenchus thornei.    
    We have studied several research articles on allelopathy, we have assessed from those that the phenomena was supposed to be phytotoxic to other nearby species, sometimes to the soil or sometimes it may be to the plant itself. The... more
    We have studied several research articles on allelopathy, we have assessed from those that the phenomena was supposed to be phytotoxic to other nearby species, sometimes to the soil or sometimes it may be to the plant itself. The secondary metabolites are the key components of allelopathic mechanism which are found in the form of plant extracts, leachates and other exudates. These biochemical strategies are useful for the defense of a plant, likewise important for the penetration of a plant into a community, attracting insects for pollination etc. Overall, allelopathy can be very effective tool for biological conservation as it does not only exert inhibition but it also aids up the healthy environment. These allelopathic compounds are very reactive and can be utilized positively to eliminate or to lower the rate of soil pollution, eutrophication and air pollution. These biochemicals can also contribute in the enhancement of soil structure and fertility, microbial and bacterial activ...
    Sequential changes induced by the root-knot nematode Meloidogyne javanica (Treub) Chitwood in mung bean ( Vigna radiata (L.) Wilczek cv. MN95) were studied. Physiological and biochemical changes were recorded 15, 30 and 45 days after... more
    Sequential changes induced by the root-knot nematode Meloidogyne javanica (Treub) Chitwood in mung bean ( Vigna radiata (L.) Wilczek cv. MN95) were studied. Physiological and biochemical changes were recorded 15, 30 and 45 days after nematode inoculation. The changes noted varied with the length of exposure to the nematode. Chlorophyll and carotenoid contents decreased in nematode-infected plants. Total phenols increased in the leaves compared with the controls for up to 30 days after inoculation. Protein content declined significantly at 30 days after exposure to the nematodes. Amylase activity was enhanced in both the leaves and the stems as compared with the controls. The results suggested that plants responded to the nematode by adopting biochemical strategies to withstand the adverse effects of infection.
    Karachi coast is heavily polluted due to the regular discharge of domestic and industrial effluents. The present study discloses the occurrence and distribution of anthropogenic pollutants in samples collected from ten different places... more
    Karachi coast is heavily polluted due to the regular discharge of domestic and industrial effluents. The present study discloses the occurrence and distribution of anthropogenic pollutants in samples collected from ten different places along the coastline of Karachi. The extent of pollution load for three consecutive years was estimated through the GIS technique. The results of the analysis revealed that physical parameters (pH, DO, salinity) were within the permissible limits whereas the level of chemical pollutants (except cyanide, As, Cr, and Cd) were exceptionally higher as compared to National Environmental Quality Standards (NEQS). On average, the study site Creek Avenue showed a higher pollution load of BOD, COD cyanide, TKN, and oil and grease. The average concentration of all heavy metals for three consecutive years (2015-2017) was found to be in an order of As<Cr<Cd<Pb<Ni. The study concluded that Karachi coastal area is overwhelmed with heavy loads of chemical...
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    This investigation was undertaken to ascertain the effect of simulated acid rain (SAR) on growth, yield and physiological parameters of tomato. SAR exposure (pH 3.0 and 4.0) caused white-to-tan spots on the abaxial and adaxial surface of... more
    This investigation was undertaken to ascertain the effect of simulated acid rain (SAR) on growth, yield and physiological parameters of tomato. SAR exposure (pH 3.0 and 4.0) caused white-to-tan spots on the abaxial and adaxial surface of tomato leaves. SAR exposure at pH 3.0 and 4.0 significantly suppressed pigment synthesis, shoot and root dry weights and yield of tomato. The
    Understanding the environmental factors that influence the rhizosphere and inner root colonization of the disease‐suppressive strains of fluorescent pseudomonads is an essential step towards improving the level and reliability of their... more
    Understanding the environmental factors that influence the rhizosphere and inner root colonization of the disease‐suppressive strains of fluorescent pseudomonads is an essential step towards improving the level and reliability of their biocontrol activity. Soil amendment with Zn at 0.8 or 1.6 mg/kg of soil alone or in combination with Pseudomonas aeruginosa IE‐6S+significantly reduced nematode penetration in tomato roots. Zn applied alone did not reduce root infection caused by Macrophomina phaseolina or Fusarium solani but did reduce when used in combination with IE‐6S+. Soil amendment with Zn at 0.8 or 1.6 mg/kg of soil alone or in conjunction with IE‐6S+ markedly suppressed Rhizoctonia solani infection. Plant height, fresh weight of shoot and protein contents of the leaves substantially improved when used with Zn, however, plants growing in the soil treated with 1.6 mg/kg of Zn in the absence of IE‐6S+ not only reduced plant growth but also showed necrotic symptoms on the leaves....
    Summary. Aqueous extracts of the four weed species Conyza canadensis, Blurnea obliqua, Amararrthus viridis and Eclipta prostrata caused substantial mortality of the plant-parasitic nematodes Xiphinema americanum, Tylenchulus semipenetrans... more
    Summary. Aqueous extracts of the four weed species Conyza canadensis, Blurnea obliqua, Amararrthus viridis and Eclipta prostrata caused substantial mortality of the plant-parasitic nematodes Xiphinema americanum, Tylenchulus semipenetrans and Meloidogyne ...
    The influence of shoot and root extracts of nine plant species including Gaillardia aristata, Cosmos bipinnatus, Helianthus annuus, Tagetes erecta, Tagetes patula, Chamomilla recutita, Matricaria discoidea, Calendula officinalis and... more
    The influence of shoot and root extracts of nine plant species including Gaillardia aristata, Cosmos bipinnatus, Helianthus annuus, Tagetes erecta, Tagetes patula, Chamomilla recutita, Matricaria discoidea, Calendula officinalis and Zinnia elegans belonging to the family Asteraceae was tested towards egg hatch and mobility of Meloidogyne javanica, the root-knot nematode juveniles in vitro. In general, root extracts of the plant species were more effective in the inhibition of nematode compared to the corresponding shoot extracts. When plant species were compared, shoot extract of Z. elegans inhibited egg hatch most while shoot extract of T. erecta caused greatest mortality of M. javanica juveniles. When compared with the controls, soil amendment with Z. elegans significantly reduced M. javanica population densities in soil and subsequent root-knot development in tomato while T. erecta failed to produce such effects. Similarly, soil amendment with Z. elegans resulted in a significant increase in plant height. Whereas both amendments enhanced fresh weight of shoot compared to the controls, none of the amendments had an influence on root growth of tomato plants.
    Effect of various fungicides on the efficacy of <i>Pseudomonas aeruginosa</i>, a plant growth-promoting rhizobacterium in the control of root-infecting fungi such as <i>Macrophomina phaseolina, Fusarium…
    Go to AGRIS search. Pakistan Journal of Plant Pathology (2003). Suppression of Meloidogyne javanica by Conyza canadensis, Blumea obliqua, Amaranthus viridis and Eclipta prostrata. ...
    To determine the influence of various trace minerals and carbon source on the biocontrol performance of Pseudomonas aeruginosa strain IE-6S+ and P. fluorescens strain CHA0 against Macrophomina phaseolina. In dual culture plate assay, P.... more
    To determine the influence of various trace minerals and carbon source on the biocontrol performance of Pseudomonas aeruginosa strain IE-6S+ and P. fluorescens strain CHA0 against Macrophomina phaseolina. In dual culture plate assay, P. aeruginosa IE-6S+ and P. fluorescens CHA0 inhibited radial growth of M. phaseolina producing zones of inhibition. Czapek's dox agar medium amended with both zinc and glucose remarkably improved antifungal activities of the bacterial inoculants. Under glasshouse conditions, soil amendment with zinc and/or glucose alone did not reduce M. phaseolina infection in tomato roots but did reduce significantly when used in combination with IE-6S+ or CHA0. Soil amendments with zinc and/or glucose increased fresh shoot weights but zinc amendment greatly reduced bacterial populations in the rhizosphere. Mineral and carbon amendments enhance the biocontrol performance of fluorescent pseudomonads against M. phaseolina. Identification of mineral and carbon amendments that favour biocontrol of certain bacterial strains may provide clues to soil factors or components of nutrient solutions in hydroponic culture that will improve the level and reliability of control.
    The aim of the present investigation was to determine the influence of nutrients on the nematicidal activity by Pseudomonas aeruginosa strain IE-6S+ and Ps. fluorescens strain CHA0 in vitro. Culture filtrate of IE-6S+ and CHA0 obtained... more
    The aim of the present investigation was to determine the influence of nutrients on the nematicidal activity by Pseudomonas aeruginosa strain IE-6S+ and Ps. fluorescens strain CHA0 in vitro. Culture filtrate of IE-6S+ and CHA0 obtained from chemically defined medium caused mortality of Meloidogyne javanica juveniles in vitro and that growth medium amended with various C, N or inorganic phosphate (Pi) sources markedly influenced nematicidal activity of the two bacteria. Glycerol (C source), propionate (fatty acid precursor) and L-lysine (N source) enhanced nematicidal activity while glucose (C), L-valine (N) and Pi substantially repressed nematicidal activity of the two bacteria. Liquid culture amendments with various C, N or Pi sources modulate the biosynthesis of nematicidal agents to a different extent in vitro. Developing bacterial strains more responsive to certain environmental signals can be exploited for increased secondary metabolite production in pharmaceutical fermentations and offers new avenues to improve biocontrol.

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