Dominique Van Der Straeten
Ghent University, Department of Physiology, Faculty Member
Research Interests:
Research Interests:
Research Interests:
High-performance liquid chromatography, coupled to tandem mass spectrometry (HPLC-MS/MS) has been established as the method of choice for the sensitive and simultaneous determination of different folates in a particular matrix, especially... more
High-performance liquid chromatography, coupled to tandem mass spectrometry (HPLC-MS/MS) has been established as the method of choice for the sensitive and simultaneous determination of different folates in a particular matrix, especially when only minute quantities of material are available. Using a previously developed and validated HPLC-MS/MS method as a starting point, we here report on the development and validation of an ultra-performance liquid chromatography (UPLC-MS/MS) method for analysis of folates in rice, which allows higher throughput and better resolution. UPLC was performed under gradient conditions on an Acquity HSS T3 column, followed by tandem mass spectrometry detection. The method was validated based on linearity, sensitivity, precision, accuracy and matrix effects. The limits of detection and the lower limits of quantification varied between 0.06 and 0.45 microg/100 g and 0.12 and 0.91 microg/100 g, respectively. Two linear calibration curves were established, one for the low and the other for the high concentration range. Analysis of the distribution and levels of folates in wild-type and folate-biofortified rice showed up to 50-fold enrichment in biofortified rice, with total folate levels of up to 900 microg/100 g rice. This is the first successful implementation of a UPLC method for the rapid and sensitive quantitative determination of folates in plant material.
Research Interests: Chemistry, Analytical Chemistry, Chromatography, Mass Spectrometry, Quantification, and 15 moreFolic acid, Medicine, Calibration, Disease, Oryza Sativa, High Performance Liquid Chromatography, Biofortification, Quantitative Analysis, Neural Tube Defects, Biochemistry and cell biology, Human Plasma, Pharmacology and pharmaceutical sciences, folates, limit of detection, and calibration curve
info:eu-repo/semantics/publishe
Research Interests:
The Arabidopsis ethylene response pathway was established by characterization of ethylene mutants that were isolated exploiting the triple response of dark-grown seedlings. The various triple response screens were not yet exhaustive;... more
The Arabidopsis ethylene response pathway was established by characterization of ethylene mutants that were isolated exploiting the triple response of dark-grown seedlings. The various triple response screens were not yet exhaustive; however, a large number of ethylene-related loci have been identified, and for several of these an allelic series was isolated. To increase the chance of identifying new loci, screening for mutants at developmental stages other than the etiolated seedling stage might be a useful approach. We have isolated mutants from light-grown populations, by using the ethylene response of nutrient-deficient seedlings at two stages in development. Characterization of these mutants has resulted in the identification of new loci involved in ethylene signaling.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
We explored the interplay between ethylene signals and the auxin pool in roots exposed to high salinity using Arabidopsisthaliana wild-type plants (Col-0), and the ethylene-signaling mutants ctr1-1 (constitutive) and ein2-1 (insensitive).... more
We explored the interplay between ethylene signals and the auxin pool in roots exposed to high salinity using Arabidopsisthaliana wild-type plants (Col-0), and the ethylene-signaling mutants ctr1-1 (constitutive) and ein2-1 (insensitive). The negative effect of salt stress was less pronounced in ctr1-1 individuals, which was concomitant with augmented auxin signaling both in the ctr1-1 controls and after 100 mM NaCl treatment. The R2D2 auxin sensorallowed mapping this active auxin increase to the root epidermal cells in the late Cell Division (CDZ) and Transition Zone (TZ). In contrast, the ethylene-insensitive ein2-1 plants appeared depleted in active auxins. The involvement of ethylene/auxin crosstalk in the salt stress response was evaluated by introducing auxin reporters for local biosynthesis (pTAR2::GUS) and polar transport (pLAX3::GUS, pAUX1::AUX1-YFP, pPIN1::PIN1-GFP, pPIN2::PIN2-GFP, pPIN3::GUS) in the mutants. The constantly operating ethylene-signaling pathway in ctr1-1 w...
Research Interests:
Inflorescence movements in response to natural gradients of sunlight are frequently observed in the plant kingdom and are suggested to contribute to reproductive success. Although the physiological and molecular bases of light-mediated... more
Inflorescence movements in response to natural gradients of sunlight are frequently observed in the plant kingdom and are suggested to contribute to reproductive success. Although the physiological and molecular bases of light-mediated tropisms in vegetative organs have been thoroughly investigated, the mechanisms that control inflorescence orientation in response to light gradients under natural conditions are not well understood. In this work, we have used a combination of laboratory and field experiments to investigate light-mediated re-orientation of Arabidopsis thaliana inflorescences. We show that inflorescence phototropism is promoted by photons in the UV and blue spectral range (≤500 nm) and depends on multiple photoreceptor families. Experiments under controlled conditions show that UVR8 is the main photoreceptor mediating the phototropic response to narrowband UV-B radiation, and phototropins and cryptochromes control the response to narrowband blue light. Interestingly, whereas phototropins mediate bending in response to low irradiances of blue, cryptochromes are the principal photoreceptors acting at high irradiances. Moreover, phototropins negatively regulate the action of cryptochromes at high irradiances of blue light. Experiments under natural field conditions demonstrate that cryptochromes are the principal photoreceptors acting in the promotion of the heliotropic response of inflorescences under full sunlight. This article is protected by copyright. All rights reserved.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests: Environmental Science, Plant Ecology, Ecosystem Services, Ecology, Forest Ecology, and 15 moreEarth and Environmental Sciences, Biotic interactions, Isoprene, Bark Beetle, Forestry Sciences, Ecosystem, Scots Pine, Norway Spruce, Monoterpenes, Semiochemicals, Boreal, Temperate Climate, Hylobius abietis, Taiga, and silver birch
Research Interests:
Several plant species require microbial associations for survival under different biotic and abiotic stresses. In this study, we show that Enterobacter sp. SA187, a desert plant endophytic bacterium, enhances yield of the crop plant... more
Several plant species require microbial associations for survival under different biotic and abiotic stresses. In this study, we show that Enterobacter sp. SA187, a desert plant endophytic bacterium, enhances yield of the crop plant alfalfa under field conditions as well as growth of the model plant Arabidopsis thaliana in vitro, revealing a high potential of SA187 as a biological solution for improving crop production. Studying the SA187 interaction with Arabidopsis, we uncovered a number of mechanisms related to the beneficial association of SA187 with plants. SA187 colonizes both the surface and inner tissues of Arabidopsis roots and shoots. SA187 induces salt stress tolerance by production of bacterial 2-keto-4-methylthiobutyric acid (KMBA), known to be converted into ethylene. By transcriptomic, genetic and pharmacological analyses, we show that the ethylene signaling pathway, but not plant ethylene production, is required for KMBA-induced plant salt stress tolerance. These res...
Research Interests:
Research Interests:
Research Interests:
Significance RNA editing is a posttranscriptional regulation process essential for organellar function. The PPR protein family plays a major role in RNA editing, but the precise function of each member and its interactions in editosome... more
Significance RNA editing is a posttranscriptional regulation process essential for organellar function. The PPR protein family plays a major role in RNA editing, but the precise function of each member and its interactions in editosome complexes remain unclear. This research demonstrates that three PPR proteins (SLO2, NUWA, and DYW2) interact in mitochondria and provides evidence for the following: ( i ) P-type PPR protein NUWA assists in the interaction between SLO2 and DYW2, and ( ii ) DYW2 participates in E + editosomes. We hypothesize that this mechanism is common in both mitochondria and chloroplasts, where the E + -type PPR proteins, such as SLO2, target the specific RNA sites, with DYW2 suggested to provide the editing catalytic domain and NUWA assisting the interaction between the former ones.
Research Interests:
Research Interests: Botany, Photosynthesis, Biology, Medicine, Gene expression, and 15 moreArabidopsis thaliana, Biological Sciences, Growth, Plant Physiology, Light, Gene ontology, Cluster Analysis, Plant growth hormone, Ethylene, Amino Acid Sequence, Cell Wall, Sodium Chloride, Gene expression profiling, Expansins, and reverse transcriptase polymerase chain reaction
Research Interests:
Research Interests:
Research Interests:
ABSTRACTPending cell death was visualized by thermographic imaging in bacterio‐opsin transgenic tobacco plants. Cell death in these plants was characterized by a complex lesion phenotype. Isolated cell death lesions were preceded by a... more
ABSTRACTPending cell death was visualized by thermographic imaging in bacterio‐opsin transgenic tobacco plants. Cell death in these plants was characterized by a complex lesion phenotype. Isolated cell death lesions were preceded by a colocalized thermal effect, as previously observed at sites infected by tobacco mosaic virus (TMV) (Chaerle et al. 1999Nature Biotechnology 17, 813–816). However, in most cases, a coherent front of higher temperature, trailed by cell death, initiated at the leaf base and expanded over the leaf lamina. In contrast to the homogenous thermal front, cell death was first visible close to the veins, and subsequently appeared as discrete spots on the interveinal tissue, as cell death spread along the veins. Regions with visible cell death had a lower temperature because of water evaporation from damaged cells. In analogy with previous observations on the localized tobacco–TMV interaction (Chaerle et al. 1999), the kinetics of thermographic and continuous gas ...
Research Interests:
Research Interests: Materials Science, Image Processing, Remote Sensing, Plant Biology, Fluorescence, and 14 moreNear Infrared, Medicine, Experimental Botany, Fruit, General Science, Health Status, Reflectivity, Fruit Quality, Image Sequence, Region of Interest, Plant Leaves, Multispectral image, Image Acquisition, and RGB-HIS color model
Research Interests:
Research Interests:
Research Interests: Chemistry, Chromatography, Food Chemistry, Folic acid, Stability, and 15 moreMedicine, Multidisciplinary, Potato, Arabidopsis thaliana, HPLC, Rice, Tandem Mass Spectrometry, Degradation, Arabidopsis, High Pressure Liquid Chromatography, Plant extracts, Solanum Tuberosum, Interconversion, Molecular Structure, and Urine Specific Gravity
Research Interests:
Research Interests: Computer Science, Global Health, United Nations, Breeding, Vitamins, and 13 moreMinerals, Developing Countries, Medicine, Multidisciplinary, Micronutrients, Policy making, Malnutrition, Humans, Food supply, Biofortification, Timeline, Nature Communications, and Biotechnology End of Life (Health Sciences)
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
chapter 1. Justification for Biofortification Introduction Biofortification is a process of increasing the density of minerals and vitamins in a food crop through conventional plant breeding, genetic engineering, or agronomic practices... more
chapter 1. Justification for Biofortification Introduction Biofortification is a process of increasing the density of minerals and vitamins in a food crop through conventional plant breeding, genetic engineering, or agronomic practices (primarily use of fertilizers and foliar sprays). Biofortified staple food crops, when substituted consistently for non-biofortified staple food crops, can generate measurable improvements in human nutrition and health. This monograph describes the progress made in developing, testing, and disseminating biofortified staple food crops, primarily through the use of conventional plant breeding, summarizing the activities of two consortiums of inter-disciplinary collaborating institutions led the HarvestPlus program and the International Potato Center (CIP). We focus on laying out the evidence base proving the effectiveness and impact to date of biofortified crops. Results of a large number of nutritional bioavailability and efficacy trials are summarized...
Research Interests:
Research Interests:
Ultraviolet (UV) radiation directly affects plants and microorganisms, but also alters the species-specific interactions between them. The distinct bands of UV radiation, UV-A, UV-B, and UV-C have different effects on plants and their... more
Ultraviolet (UV) radiation directly affects plants and microorganisms, but also alters the species-specific interactions between them. The distinct bands of UV radiation, UV-A, UV-B, and UV-C have different effects on plants and their associated microorganisms. While UV-A and UV-B mainly affect morphogenesis and phototropism, UV-B and UV-C strongly trigger secondary metabolite production. Short wave (<350 nm) UV radiation negatively affects plant pathogens in direct and indirect ways. Direct effects can be ascribed to DNA damage, protein polymerization, enzyme inactivation and increased cell membrane permeability. UV-C is the most energetic radiation and is thus more effective at lower doses to kill microorganisms, but by consequence also often causes plant damage. Indirect effects can be ascribed to UV-B specific pathways such as the UVR8-dependent upregulated defense responses in plants, UV-B and UV-C upregulated ROS accumulation, and secondary metabolite production such as phe...
Research Interests:
SummaryRice is a major food crop to approximately half of the human population. Unfortunately, the starchy endosperm, which is the remaining portion of the seed after polishing, contains limited amounts of micronutrients. Here, it is... more
SummaryRice is a major food crop to approximately half of the human population. Unfortunately, the starchy endosperm, which is the remaining portion of the seed after polishing, contains limited amounts of micronutrients. Here, it is shown that this is particularly the case for thiamin (vitamin B1). Therefore, a tissue‐specific metabolic engineering approach was conducted, aimed at enhancing the level of thiamin specifically in the endosperm. To achieve this, three major thiamin biosynthesis genes, THIC, THI1 and TH1, controlled by strong endosperm‐specific promoters, were employed to obtain engineered rice lines. The metabolic engineering approaches included ectopic expression of THIC alone, in combination with THI1 (bigenic) or combined with both THI1 and TH1 (trigenic). Determination of thiamin and thiamin biosynthesis intermediates reveals the impact of the engineering approaches on endosperm thiamin biosynthesis. The results show an increase of thiamin in polished rice up to th...
Research Interests:
Research Interests:
Research Interests:
We demonstrate the identification and application of molecular reporters and phenotypic traits to monitor the dynamics of the still largely unknown UVR8-dependent signalling pathways.
Research Interests:
The force of gravity is a constant environmental factor. Plant shoots respond to gravity through negative gravitropism and gravity resistance. These responses are essential for plants to direct the growth of aerial organs away from the... more
The force of gravity is a constant environmental factor. Plant shoots respond to gravity through negative gravitropism and gravity resistance. These responses are essential for plants to direct the growth of aerial organs away from the soil surface after germination and to keep an upright posture above ground. We took advantage of the effect of brassinosteroids on the two types of graviresponses inArabidopsis thalianahypocotyls to disentangle functions of cell wall polymers during etiolated shoot growth. The ability of etiolated Arabidopsis seedlings to grow upwards was suppressed in the presence of 24-epibrassinolide (EBL) but enhanced in the presence of brassinazole (BRZ), an inhibitor of brassinosteroid biosynthesis. These effects were accompanied by changes in cell wall mechanics and composition. Cell wall biochemical analyses and confocal microscopy of the cellulose-specific pontamine S4B dye revealed that the EBL and BRZ treatments correlated with changes in cellulose fibre or...