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Photosynthesis
• photosynthesis converts light energy to the chemical energy of food.
• In eukaryotes that are autotrophs, photosynthesis occurs in chloroplasts.
• Chloroplasts, organelles containing thylakoids.
• Stacks of thylakoids form grana.
• Photosynthesis is summarized as
6 CO2 + 12 H2O + Light Energy  C6H12O6 + 6 O2 + 6 H2O.
• Photosynthesis is a redox process: H2O is oxidized, and CO2 is reduced.
• The light reactions in the thylakoid membranes split water, releasing O2 ,
producing ATP, and forming NADPH.
• The Calvin cycle in the stroma forms sugar from CO2 using ATP for energy and
NADPH for reducing power.
Chloroplast
Chlorophyll
Light Spectrum
Light Reaction
• The light dependent reactions use photosynthetic pigments (organized
into photosystems) to convert light energy into chemical energy
(specifically ATP and NADPH).
• Photosystems are groups of photosynthetic pigments (including
chlorophyll) embedded within the thylakoid membrane.
• Photosystems are classed according to their maximal absorption
wavelengths (PS I = 700 nm ; PS II = 680 nm).
• When a photosystem absorbs light energy, delocalized electrons within
the pigments become energized or ‘excited’.
• These excited electrons are transferred to carrier molecules within the
thylakoid membrane.
Light Reaction
• Excited electrons from Photosystem II (P680) are transferred to an electron
transport chain within the thylakoid membrane.
• As the electrons are passed through the chain they lose energy, which is used to
translocate H+ ions into the thylakoid.
• This build up of protons within the thylakoid creates an electrochemical
gradient, or proton motive force.
• The H+ ions return to the stroma (along the proton gradient) via the
transmembrane enzyme ATP synthase (chemiosmosis).
• ATP synthase uses the passage of H+ ions to catalyze the synthesis of ATP (from
ADP + Pi).
• This process is called photophosphorylation – as light provided the initial
energy source for ATP production
• The newly de-energized electrons from Photosystem II are taken up by
Photosystem I.
Light Reaction
• Excited electrons from Photosystem is transferred to a carrier molecule
and used to reduce NADP+
• This forms NADPH – which is needed (in conjunction with ATP) for the
light independent reactions.
• The electrons lost from Photosystem I are replaced by de-energized
electrons from Photosystem II.
• The electrons lost from Photosystem II are replaced by electrons released
from water via photolysis.
• Water is split by light energy into H+ ions (used in chemiosmosis) and
oxygen (released as a by-product).
Dark Reactions
• The light independent reactions use the chemical energy derived from light
dependent reactions to form organic molecules.
• The light independent reactions occur in the fluid-filled space of the
chloroplast called the stroma.
• The light independent reactions are collectively known as the Calvin cycle
and involve three main steps.
• The Calvin cycle begins with a 5C compound called ribulose biphosphate
(or RuBP).
• An enzyme, RuBP carboxylase (or Rubisco), catalyzes the attachment of a
CO2 molecule to RuBP.
• The resulting 6C compound is unstable and breaks down into two 3C
compounds – called glyceraldehyde-3-phosphate (GP).
• A single cycle involves three molecules of RuBP combining with three
molecules of CO2 to make six molecules of GP.
Dark Reactions
• Glycerate-3-phosphate (GP) is converted into triose phosphate (TP) using
NADPH and ATP.
• Reduction by NADPH transfers hydrogen atoms to the compound, while the
hydrolysis of ATP provides energy.
• Each GP requires one NADPH and one ATP to form a triose phosphate – so a
single cycle requires six of each molecule.
• Of the six molecules of TP produced per cycle, one TP molecule may be used to
form half a sugar molecule.
• Hence two cycles are required to produce a single glucose monomer, and more
to produce polysaccharides like starch.
• The remaining five TP molecules are recombined to regenerate stocks of
RuBP (5 × 3C = 3 × 5C).
• The regeneration of RuBP requires energy derived from the hydrolysis of ATP.
Calvin
Cycle
Photosynthesis.pptx

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Photosynthesis.pptx

  • 1. Photosynthesis • photosynthesis converts light energy to the chemical energy of food. • In eukaryotes that are autotrophs, photosynthesis occurs in chloroplasts. • Chloroplasts, organelles containing thylakoids. • Stacks of thylakoids form grana. • Photosynthesis is summarized as 6 CO2 + 12 H2O + Light Energy  C6H12O6 + 6 O2 + 6 H2O. • Photosynthesis is a redox process: H2O is oxidized, and CO2 is reduced. • The light reactions in the thylakoid membranes split water, releasing O2 , producing ATP, and forming NADPH. • The Calvin cycle in the stroma forms sugar from CO2 using ATP for energy and NADPH for reducing power.
  • 5. Light Reaction • The light dependent reactions use photosynthetic pigments (organized into photosystems) to convert light energy into chemical energy (specifically ATP and NADPH). • Photosystems are groups of photosynthetic pigments (including chlorophyll) embedded within the thylakoid membrane. • Photosystems are classed according to their maximal absorption wavelengths (PS I = 700 nm ; PS II = 680 nm). • When a photosystem absorbs light energy, delocalized electrons within the pigments become energized or ‘excited’. • These excited electrons are transferred to carrier molecules within the thylakoid membrane.
  • 6. Light Reaction • Excited electrons from Photosystem II (P680) are transferred to an electron transport chain within the thylakoid membrane. • As the electrons are passed through the chain they lose energy, which is used to translocate H+ ions into the thylakoid. • This build up of protons within the thylakoid creates an electrochemical gradient, or proton motive force. • The H+ ions return to the stroma (along the proton gradient) via the transmembrane enzyme ATP synthase (chemiosmosis). • ATP synthase uses the passage of H+ ions to catalyze the synthesis of ATP (from ADP + Pi). • This process is called photophosphorylation – as light provided the initial energy source for ATP production • The newly de-energized electrons from Photosystem II are taken up by Photosystem I.
  • 7. Light Reaction • Excited electrons from Photosystem is transferred to a carrier molecule and used to reduce NADP+ • This forms NADPH – which is needed (in conjunction with ATP) for the light independent reactions. • The electrons lost from Photosystem I are replaced by de-energized electrons from Photosystem II. • The electrons lost from Photosystem II are replaced by electrons released from water via photolysis. • Water is split by light energy into H+ ions (used in chemiosmosis) and oxygen (released as a by-product).
  • 8.
  • 9. Dark Reactions • The light independent reactions use the chemical energy derived from light dependent reactions to form organic molecules. • The light independent reactions occur in the fluid-filled space of the chloroplast called the stroma. • The light independent reactions are collectively known as the Calvin cycle and involve three main steps. • The Calvin cycle begins with a 5C compound called ribulose biphosphate (or RuBP). • An enzyme, RuBP carboxylase (or Rubisco), catalyzes the attachment of a CO2 molecule to RuBP. • The resulting 6C compound is unstable and breaks down into two 3C compounds – called glyceraldehyde-3-phosphate (GP). • A single cycle involves three molecules of RuBP combining with three molecules of CO2 to make six molecules of GP.
  • 10. Dark Reactions • Glycerate-3-phosphate (GP) is converted into triose phosphate (TP) using NADPH and ATP. • Reduction by NADPH transfers hydrogen atoms to the compound, while the hydrolysis of ATP provides energy. • Each GP requires one NADPH and one ATP to form a triose phosphate – so a single cycle requires six of each molecule. • Of the six molecules of TP produced per cycle, one TP molecule may be used to form half a sugar molecule. • Hence two cycles are required to produce a single glucose monomer, and more to produce polysaccharides like starch. • The remaining five TP molecules are recombined to regenerate stocks of RuBP (5 × 3C = 3 × 5C). • The regeneration of RuBP requires energy derived from the hydrolysis of ATP.