This document discusses biofuels as a sustainable alternative to fossil fuels. It defines biofuels as liquid fuels produced through biological processes from biomass sources. The document outlines four generations of biofuels from first generation using food crops, to fourth generation using non-arable land. The main types of biofuels - bioethanol, biodiesel, biogas, and algal-based biofuels - are described. Microbes play an important role in producing and refining biofuels from oils, carbohydrates, and proteins. The document concludes that continued advances in biofuels will help conserve non-renewable resources and lead to a more sustainable energy future.