The cultivated wheat, Triticum aestivum, is one of the most important staple crops in the world. There has been great interest in the determination of ancestral diploid genome donors of T. aestivum ― an allohexaploid, because of agronomic... more
The cultivated wheat, Triticum aestivum, is one of the most important staple crops in the world. There has been great interest in the determination of ancestral diploid genome donors of T. aestivum ― an allohexaploid, because of agronomic significance. Southeastern Turkey in Karacadag District of the Diyarbakir province, exhibits great genetic diversity in terms of Triticeae family plants, where wheat was originally domesticated. In order to better understand the phyletical relationships between T. aestivum and its possible ancestral genome donors, tissue samples were collected from 38 individuals belonging to four species of Triticum and two subspecies of Aegilops speltoides, distributed throughout the above-mentioned geographical area. The ITS1, ITS2 and 5.8S rDNA repeat segments from these plants were sequenced and the nucleotide sequences were phyletically analysed. The results postulated that Triticum monococcum var. boeoticum is likely to be an a genome donor of both, Triticum dicoccoides (AABB) and Triticum aestivum (AABBDD). The results further support Ae. speltoides as a possible B genome source.
Research Interests:
Abstract: The present paper describes whether the efficiency of gene transfer from Agrobacterium tumefaciens to plant cells is influenced by the location of npt-II marker gene in T-DNA region. Therefore, binary vectors pSCV-Ori1 and... more
Abstract: The present paper describes whether the efficiency of gene transfer from Agrobacterium tumefaciens to plant cells is influenced by the location of npt-II marker gene in T-DNA region. Therefore, binary vectors pSCV-Ori1 and pSCV-Ori2 were constructed and used for ...
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul, TURKEYWOS:000295310800420Cowpea (Vigna unguiculataL.) is one of the most importantdrought-tolerant summer food legume crop largely cultivated ontens of millions of small... more
European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul, TURKEYWOS:000295310800420Cowpea (Vigna unguiculataL.) is one of the most importantdrought-tolerant summer food legume crop largely cultivated ontens of millions of small farms in the drier zones of Africa withlimited yield due to various biotic and abiotic stresses. It is alsogrown in some Asian, South European, Central and South Ameri-can countries both as food and forage crops. Cowpea can play animportant role in supplementing rural population diet due to itslow cost high protein, high calories, minerals and vitamins. Worlddemand for legume feed protein is increasing gradually due toincreasing demand for animal food products. Therefore, there is adire need to develop new varieties combining traditional and mod-ern biotechnological techniques to meet these future challenges.Reports concerning micropropagation and tissue culture studies ofcowpea started during early 1980s with moderate success in 1990sowing to recalcitrant nature of plant. However, during 2000s manyresearchers developed new protocols to overcome the problemof recalcitrance and consequently micropropagation. This reportreviews past and present tissue culture and genetic transformationresearch on cowpea and also focus on future prospects of cowpearesearch using modern biotechnological techniquesEuropean Biotechnol Themat Network Asso
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Peganum harmala L., a perennial shrub of medicinal and ornamental importance, was propagated from shoot apices and first axillary buds on agar solidified MS medium supplemented with 0-2.22 μM benzylaminopurine (BAP) in combination with... more
Peganum harmala L., a perennial shrub of medicinal and ornamental importance, was propagated from shoot apices and first axillary buds on agar solidified MS medium supplemented with 0-2.22 μM benzylaminopurine (BAP) in combination with 0.11–1.07 μM α-naphthalene acetic acid (NAA). No shoot regeneration was observed on MS medium without plant growth regulators. MS medium containing 1.11 μM BAP and 0.11 μM NAA was optimal for shoot regeneration from both explant types at 16 h light. Shoot ...
Research Interests:
Research Interests: Botany, Biology, Micropropagation, Medicine, Multidisciplinary, and 15 moreBiological Sciences, Flowers, First record, Germination, Critically Endangered, Plant Regeneration, Mediterranean region, Plant species, Conservation of Natural Resources, Naturwissenschaften, Embryos, Centaurea, In Vitro Propagation, Murashige and Skoog, and Kinetin
There is much data to indicate that only a small number of cells in plant explants are competent for stable transformation by Agrobacterium. Circumstantial evidence suggests that certain cells reentering cell division at wound sites are... more
There is much data to indicate that only a small number of cells in plant explants are competent for stable transformation by Agrobacterium. Circumstantial evidence suggests that certain cells reentering cell division at wound sites are competent for transformation by Agrobacterium. We have discovered a member of the intracellular PR gene family from asparagus (AoPR1) which is strongly expressed upon wounding and during the reactivation of the cell cycle in cultured asparagus cells, but which shows very little expression in intact plant tissues. The promoter from the AoPR1 gene was fused to an intron-containing GUS reporter gene and shown to be more strongly expressed than the commonly used CaMV 35S constitutive promoter in target cells for plant transformation. A transcriptional fusion of the AoPR1 promoter with an NPT-II gene was found to be a very efficient marker for the selection of transgenic tobacco callus. Expression of the AoPR1-NPT-II gene allowed efficient shoot formation on transgenic callus and efficient adventitious root formation on transgenic shoots. These latter observations provided firm evidence that transformation selection marker gene expression is most crucial at the early stages of the transformation process, during the establishment of transformed micro-calli.
Research Interests: Genetics, Plant Biology, Biology, Cell Cycle, Morphogenesis, and 15 morePlant Molecular Biology, Gene expression, Cell Division, Genetic Engineering, Molecular, Plant Transformation, Plant, Glucuronidase, Base Sequence, Gene Family, PLANT PROTEINS, Biochemistry and cell biology, Gene Expression Regulation, Molecular Sequence Data, and Agrobacterium tumefaciens
Sternbergia fischeriana is an endangered geophyte and therefore in vitro micropropagation of this plant will have great importance for germplasm conservation and commercial production. Bulb scale and immature embryo explants of S.... more
Sternbergia fischeriana is an endangered geophyte and therefore in vitro micropropagation of this plant will have great importance for germplasm conservation and commercial production. Bulb scale and immature embryo explants of S. fischeriana were cultured on ...
Research Interests:
A rapid and simple method for adventitious shoot regeneration and somatic embryogenesis from immature cotyledon explants of pea (Pisum sativum L.) is described. Cotyledon size and the explant orientation to the medium surface were shown... more
A rapid and simple method for adventitious shoot regeneration and somatic embryogenesis from immature cotyledon explants of pea (Pisum sativum L.) is described. Cotyledon size and the explant orientation to the medium surface were shown to have a clear effect on shoot regeneration. The highest frequency of shoot regeneration was achieved when the distal end of the greenest cotyledons (7–8 mm in size) were placed in contact with the agar surface. Shoots rooted at a frequency of 80–90% and grew into normal fertile plants. Somatic embryos were induced in cultures of immature cotyledons on modified MS medium containing high levels of a-naphthaleneacetic acid (27–215 µM) and 2,4-dichlorophenoxyacetic acid (23–181 µM). A higher frequency of somatic embryos with a normal morphology were induced using a-naphthaleneacetic acid.
Research Interests:
Research Interests:
Research Interests:
... Dekstroz ucuz bir karbonhidrat kaynağı olup, sakız gibi ürünleri hafif tatlandırır. Reçel, jel ve dondurma karışımlarında sukrozun yoğun tatlandırma özelliğini hafifletir venemi muhafaza ederek ürünün bayatlamasını engeller. ...
Research Interests:
Randomly Amplified Polymorphic DNA markers (RAPD) were used to assess the hybrid identity of individuals sampled as Phlomis x termessi Davis. Out of 95 primers screened, 11 primers produced reproducible amplification patterns used for... more
Randomly Amplified Polymorphic DNA markers (RAPD) were used to assess the hybrid identity of individuals sampled as Phlomis x termessi Davis. Out of 95 primers screened, 11 primers produced reproducible amplification patterns used for discrimination of P. x termessi and their parents. Eleven primers produced 81 bands. Forty two percent of the RAPD bands existed in parents. Of the 54 bands found in P. lycia, 19 were found only in this species and 7 of these were monomorphic. Similarly, of 57 RAPD bands observed in P. bourgaei, 18 were found only in P. bourgaei and 6 of these were monomorphic. Among hybrid individuals, 35 of the 73 markers were monomorphic. Fifteen of these existed in individual parents showing that parents were homozygous for these markers. Of the 35 monomorphic bands observed among hybrid individuals, 5 were present in the samples of one of the parents and completely absent from the samples of the other; therefore, additive inheritance is indicated. Of the 5 additive bands, 1 was inherited from P. bourgaei and 4 were inherited from P. lycia. Among 38 polymorhic markers observed in hybrid individuals, 9 were new and hybrid-specific. Pollen fertility was also investigated. Mean pollen fertility for P. lycia and P. bourgaei was 93% and 97% respectively. However, mean pollen fertility for hybrids was 65% (+/-10.5).
Research Interests:
Randomly Amplified Polymorphic DNA markers were used to determine the genetic relationships among Turkish lentil cultivars and breeding lines. Fourteen cultivars and thirteen breeding lines were evaluated to determine genetic variability... more
Randomly Amplified Polymorphic DNA markers were used to determine the genetic relationships among Turkish lentil cultivars and breeding lines. Fourteen cultivars and thirteen breeding lines were evaluated to determine genetic variability using nine random 10-mer primers (among 45 primers). Forty-one reproducible bands were obtained, 54% of which were polymorphic. Genetic distances among cultivars and breeding lines were obtained from the
Research Interests:
Research Interests:
Efficient and reproducible shoot regeneration has been established from immature cotyledons and embryo axes of Hungarian vetch. Immature embryo axes showed higher regeneration capacity than immature cotyledons in most media tested.... more
Efficient and reproducible shoot regeneration has been established from immature cotyledons and embryo axes of Hungarian vetch. Immature embryo axes showed higher regeneration capacity than immature cotyledons in most media tested. Immature ...
Research Interests:
Multiple shoots from shoot meristems of three - five-day-old in vitro grown seedlings of Turkish cowpea (Vigna unguiculata L.) cv. Akkiz was obtained in MS supplemented with 0.50 mg/l BAP - 0, 0.10, 0.30 and 0.50 mg/l NAA. Callus... more
Multiple shoots from shoot meristems of three - five-day-old in vitro grown seedlings of Turkish cowpea (Vigna unguiculata L.) cv. Akkiz was obtained in MS supplemented with 0.50 mg/l BAP - 0, 0.10, 0.30 and 0.50 mg/l NAA. Callus induction was recorded on all cultures. Callusing was recorded on all cultures containing 0.5 mg/l BAP with and without NAA. However, increased diameter of calli was recorded on MS medium containing 0.5 mg/l BAP - 0.1, 0.3 and 0.5 mg/l NAA. The highest frequency (%) of shoot regeneration and mean number of shoots per explant was recorded on MS medium containing 0.5 mg/l BAP with out NAA. Addition of any concentration of NAA resulted in significant decrease in the frequency (%) of shoot regeneration and mean number of shoots per explant. Maximum mean number of 2.60 shoots per explant was obtained on MS without NAA. Regenerated shoots were rooted on MS containing 0.50 mg/l IBA where up to seven adventitous secondary shoots arose from the base of mother shoot ...
Research Interests:
Cowpea is an important grain legume crop. The study reports an efficient in vitro multiplication and shoots regeneration protocol from preconditioned embryonic axes of the Turkish cowpea cultivar Akkiz. The embryonic axes were... more
Cowpea is an important grain legume crop. The study reports an efficient in vitro multiplication and shoots regeneration protocol from preconditioned embryonic axes of the Turkish cowpea cultivar Akkiz. The embryonic axes were preconditioned with 10 mg/l BA on agar solidified MS medium for 5 days. Thereafter they were cultured on MS medium containing 0.25, 0.50, 0.75 and 1.00 mg/l BA with or without 0.10 mg/l NAA. Mean frequency (%) of shoot regeneration, number of shoots per explant and shoot length decreased with each increase in BA concentration used singly. However, a positive increase was recorded in all parameters in the presence of 0.10 mg/l NAA in the regeneration medium. A maximum mean number of 10.33 shoots per explant was recorded on an MS medium containing 1.00 mg/l BA -0.1 mg/l NAA. Regenerated shoots were rooted on an MS medium containing 0.50 mg/l IBA. Rooted plants were acclimatized at room temperature in soil mix contained in pots where they were subjected to an int...
Research Interests:
The hairy vetch (Vicia villosa Roth) is a climbing, prostrate or trailing legume grown as forage.It fixes atmospheric nitrogen, reduces soil erosion and provides an instant mulch. Multiple axillary shoot regeneration from a mature seed... more
The hairy vetch (Vicia villosa Roth) is a climbing, prostrate or trailing legume grown as forage.It fixes atmospheric nitrogen, reduces soil erosion and provides an instant mulch. Multiple axillary shoot regeneration from a mature seed explant (zygotic embryo with two cotyledons) was obtained on MS medium containing 0.05 - 1.6 mg/l TDZ with or without 0.10 mg/l IBA. The frequency (%) of shoot regeneration ranged from 45.83-75.00% with a maximum number of 28.6 shoots per explant on MS medium containing 0.20 mg/l TDZ-0.10 mg/l IBA. The mean shoot length decreased proportionately with each increase in TDZ concentration irrespective of the IBA concentration in the culture medium. However, comparing the two types of regeneration media, longer shoots were recorded in the presence of IBA in the culture medium. Regenerated shoots were pulse treated with 50 mg/l IBA for 5, 10 and 20 min for rooting.
Research Interests:
... 12 PB246 100 63 6-7 Erected 4-5 3 13 PB248 102 65 6-7 Erected 2-4 3 14 PP60 50 25 4-5 Semi-erected None 2 15 PP70 65 60 6 Erected 3 3 ... 31 U3 80 35 5-6 Bent over None 2 32 U12 45 25 4-6 Semi-erected None 2 33 U19 55 28 2-6 Erected... more
... 12 PB246 100 63 6-7 Erected 4-5 3 13 PB248 102 65 6-7 Erected 2-4 3 14 PP60 50 25 4-5 Semi-erected None 2 15 PP70 65 60 6 Erected 3 3 ... 31 U3 80 35 5-6 Bent over None 2 32 U12 45 25 4-6 Semi-erected None 2 33 U19 55 28 2-6 Erected None 2 ...
Özet Fasulye (Phaseolus vulgaris L.) dünya verilerine göre en fazla ekim alanına ve üretime sahip, yararlanma ve kullanma bakımından özellikle proteince zengin bir yemeklik tane baklagil bitkisidir. Fasulyenin Aras ve Eskişehir-855... more
Özet Fasulye (Phaseolus vulgaris L.) dünya verilerine göre en fazla ekim alanına ve üretime sahip, yararlanma ve kullanma bakımından özellikle proteince zengin bir yemeklik tane baklagil bitkisidir. Fasulyenin Aras ve Eskişehir-855 çeşitleri laboratuvar koşullarında çimlendirilmiş ve dört yapraklı döneme geldiklerinde gövdeleri Agrobacterium tumefaciens' in onkogenik A281 hattı ve non-onkogenik GV2260, EHA105 hatları ile aşılanmıştır. Aşılama sonucunda A281 ile muamele edilmiş Aras ve Eskişehir-855 çeşitlerinde tümörler ...
Research Interests:
Research Note<br><br>Plant regeneration <i>in vitro</i> from immature embryos of lesser burnet (<i>Sanguisorba minor</i> Scop.).
Triticale is an important cereal crop grown throughout the world. The study reports somatic embryogenesis from immature zygotic embryos of 5 Turkish triticale genotypes. The explants were initially cultured on MS medium supplemented with... more
Triticale is an important cereal crop grown throughout the world. The study reports somatic embryogenesis from immature zygotic embryos of 5 Turkish triticale genotypes. The explants were initially cultured on MS medium supplemented with 2 mg dm-3 2, 4-D, 500 mg dm-3 glutamine, 100 mg dm-3 casein hydrolysate, 2% sucrose and 7% agar for two weeks in the dark. The temperature was maintained at 24±2ºC. Thereafter, the developing embryogenic calli were transferred to MS medium without 2, 4-D to achieve ...
Research Interests:
Mass proliferation from the lower half bulb scales and adventitious bulb regeneration from in vitro cultured leaf explants of Madonna Lilly (Lilium candidum L.) was achieved using various concentrations of BAP-IBA. Regenerated plantlets... more
Mass proliferation from the lower half bulb scales and adventitious bulb regeneration from in vitro cultured leaf explants of Madonna Lilly (Lilium candidum L.) was achieved using various concentrations of BAP-IBA. Regenerated plantlets were transferred to greenhouse for adaptation where they flowered after two years.
Research Interests:
(English) The study examined mutagenic effects of 0.001-0.005 M NaN3 with or without BAP or TDZ in MS medium on M1 generation of four pea cultivars Winner, Sprinter, Bolero and Karina. LD50 results showed that 0.001 M NaN3 was most... more
(English) The study examined mutagenic effects of 0.001-0.005 M NaN3 with or without BAP or TDZ in MS medium on M1 generation of four pea cultivars Winner, Sprinter, Bolero and Karina. LD50 results showed that 0.001 M NaN3 was most appropriate for the creation of mutagenesis and was not highly lethal. Seeds treated with 0.001 M NaN3 grown on MS medium containing various concentrations of Benzylaminopurine (BAP) and Thidiazuron (TDZ) showed that BAP with NaN3 was better compared to TDZ with NaN3. ...
Research Interests:
Four lentil cultivars (Lens culinaris Medik) were treated with super virulent strain A281 (pTiBo 542) pBI121. 1 of Agrobacterium tumefaciens and strain 15834 (pR 15834) of A. rhizogenes to evaluate their behavior towards tumor formation... more
Four lentil cultivars (Lens culinaris Medik) were treated with super virulent strain A281 (pTiBo 542) pBI121. 1 of Agrobacterium tumefaciens and strain 15834 (pR 15834) of A. rhizogenes to evaluate their behavior towards tumor formation and rooting respectively. High frequency of tumor formation was observed from cotyledon node and with variable tumor formation on shoot meristems, which bore shoots in all cultivars. Rooting was only observed in cultivar Erzurum 89. A. rhizogenes was noted to induce roots in dark. Light ...
Research Interests:
The effect of pre-sowing seed treatment with zinc and foliar sprays of humic acid on yield and protein contents of common bean (Phaseolus vulgaris L.) were investigated under semi arid conditions of Central Anatolia. It was observed that... more
The effect of pre-sowing seed treatment with zinc and foliar sprays of humic acid on yield and protein contents of common bean (Phaseolus vulgaris L.) were investigated under semi arid conditions of Central Anatolia. It was observed that seed pretreatment with Zn or pre treatment of seeds+ foliar spray of humic acid substances at three to six leaf stage significantly increased yield and yield components in common bean. Correlation coefficients of various agronomic characteristics showed that a unit increase in common bean seed ...
Research Interests:
Page 1. 21 AsPac J. Mol. Biol. Biotechnol., Vol. 12 (1&amp;amp;2), 2004 In Vitro Micropropagation of Ludwigia repens Asia Pacific Journal of Molecular Biology and Biotechnology, 2004 Vol. 12 (1&amp;amp;2) : 21-25 *Author for... more
Page 1. 21 AsPac J. Mol. Biol. Biotechnol., Vol. 12 (1&amp;amp;2), 2004 In Vitro Micropropagation of Ludwigia repens Asia Pacific Journal of Molecular Biology and Biotechnology, 2004 Vol. 12 (1&amp;amp;2) : 21-25 *Author for Correspondence. ...
Research Interests:
Research Interests:
Research Interests:
Some morphologic characteristics of five sainfoin species, section Heliobrychis collected from natural flora of various regions of Turkey were analysed based on phenotypic variations. Generally, the shoots and types of leaves, raceme,... more
Some morphologic characteristics of five sainfoin species, section Heliobrychis collected from natural flora of various regions of Turkey were analysed based on phenotypic variations. Generally, the shoots and types of leaves, raceme, flower, fruit, and type of seeds were analyzed in the study. High variation was recorded among morphologic characters of sainfoin. Hierarchical Cluster analysis showed relationship between the species due to morphological characteristics ending up with three groups. It is assumed that these results will be beneficial for breeding of sainfoin in the future.
Research Interests:
Abstract not available
Research Interests:
Abstract Grass pea is very sensitive to the deficiency or excessive amount of micronutrient elements like Fe, Zn, B, Mo that could disturb metabolic activities of the plants including inhibition in growth and their death. There is lack of... more
Abstract Grass pea is very sensitive to the deficiency or excessive amount of micronutrient elements like Fe, Zn, B, Mo that could disturb metabolic activities of the plants including inhibition in growth and their death. There is lack of information about the influence of these elements increased concentration in grass pea growth under in vitro conditions. Therefore, the present study aimed to investigate the availability and regeneration capacity of grass pea cv. Gurbuz using 1× (control), 2×, 3× 4× and 5× concentration of Fe, Zn, B and Mo in MS medium containing 1 mg/L thidiazuron and 0.25 mg/L naphthaleneacetic acid. The results demonstrated significant variations in regeneration capacity of the cultivar in respective treatments and showed inhibited response to enhanced concentration of Fe, Zn, B, and Mo supplies. All enhanced concentrations of Fe, Zn, B, and Mo treatments in MS medium resulted in poor shoot induction and reduced shoot counts per plants as compared to control. Each increase in concentration of Fe and B significantly increased shoot length, number of nodes/plants and internode lengths. Whereas, significant decrease in same traits were noted on MS medium containing enhanced concentrations of Zn and Mo. Root induction was completely inhibited using enhanced concentration of Fe and B. Whereas, root induction was promoted by Zn and Mo micronutrients in MS medium. The rooted shoots from Fe and Zn micronutrients content induced early flowering and maturity during hardening. The results showed that optimum micronutrient supply are key factors in successful regeneration of grass pea under culture conditions.
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Borulce (Vigna unguiculata L.) onemli bir yemeklik baklagil bitkisi olup, bircok ulkede cesitli sekilde tuketilmektedir. Bu calismada farkli konsantrasyonlarda sitokinin ve oksin iceren MS besin ortami kullanilarak Akkiz ve Karagoz... more
Borulce (Vigna unguiculata L.) onemli bir yemeklik baklagil bitkisi olup, bircok ulkede cesitli sekilde tuketilmektedir. Bu calismada farkli konsantrasyonlarda sitokinin ve oksin iceren MS besin ortami kullanilarak Akkiz ve Karagoz borulce cesitlerinin tam ve yarim kotiledon bogum, surgun ucu, olgunlasmamis kotiledon, plumula ve embriyonik eksen eksplantlarindan surgun rejenerasyonu elde edilmistir. Eksplant basina en fazla (10.8 adet) surgun embriyonik eksenden 1.00 mg/l BAP ve 0.1 mg/l NAA iceren MS ortamindan elde edilmistir. Genel olarak ortama NAA ilavesi surgun rejenerasyon oranini ve eksplant basina surgun sayisini olumsuz etkilerken, surgun uzunlugunu olumlu sekilde etkilenmistir. Elde edilen surgunler 0.5 mg/l IBA iceren MS ortaminda koklendirilerek serada dis sartlarda alistirilip tohum elde edilmistir. Calismanin 2. asamasinda markor genleri (NPTII ve GUS), heRbisitlere (bar) ve boceklere dayaniklilik (cry 1C, cry ICST, cry 2A, cry 2 AST, cry 1AB ve hibrit SN19) genlerini tasiyan Agrobacterium tumefaciens’in GV2260, LBA 4404 ve AG10 hatlari kullanarak transgenik bitkiler seleksiyon ortaminda secilmistir. Farkli genleri tasiyan cok sayida secilmis transgenik aday surgunler 0.5 mg/l IBA, 50 mg/l kanamisin ve 500 mg/l Augmentin iceren MS ortamda koklendirilmistir. Koklendirilen bitkiler serada dis sartlarda alistirilip tohum elde edilmistir. Degisik primerler kullanarak PCR analizi ile transgenik bitkiler teyit edilmistir.Abstract Cowpea (Vigna unguiculata L.), an important food grain legume crop, is consumed in many countries in different ways. The study reports in vitro shoot regeneration from halved cotyledon node, shoot meristem, immature cotyledon, plumule and embryonic axis explants of cv. Akkiz and Karagoz of cowpea on MS medium containing various concentrations of cytokinins and auxins. Highest number of 10. 08 shoots per explant were recorded on MS medium containing 1.00 mg/l BAP and 0.1 mg/l NAA from embryonic axis. Inclusion of NAA in the culture medium generally had negative effect on frequency of shoot regeneration and number of shoots per explant but had positive effect on shoot length. Regenerated shoots were rooted on MS medium containing 0.50 mg/l IBA and acclimatized in the greenhouse, where they set seeds. In the second phase of study, Agrobacterium tumefaciens strains GV2260, LBA4404 and AG10 containing markor (NPT II ve GUS), herbicide resistant (bar) and insect resistant (cry 1C, cry 1CST, cry 2A, cry 2AST, cry 1AB and hybrid SN19 genes were used to select putative transgenic plants in medium containing kanamycin. Putative transgenic shoots regenerated on different media from various explants were rooted on MS selection medium containing 0.50 mg/l IBA, 50 mg/l kanamisin and 500 mg/l Augmentin. Rooted plants were acclimatised in the greenhouse and seeds were obtained. Putative transgenic plants were confirmed by PCR analysis using different primers.