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Üreme biyoteknolojisi alanında hayvanlardan elde edilen spermayı uzun süreli saklamak için pek çok yöntem geliştirilmiştir. Bu yöntemler birbirleri arasında kıyaslandığında avantajları ve dezavantajları ortaya çıkmaktadır. Kriyobiyolojik... more
Üreme biyoteknolojisi alanında hayvanlardan elde edilen spermayı uzun süreli saklamak için pek çok yöntem geliştirilmiştir. Bu yöntemler birbirleri arasında kıyaslandığında avantajları ve dezavantajları ortaya çıkmaktadır. Kriyobiyolojik açıdan hala eksikliklerin olduğu, bunun için spermanın dondurulmasında güncel metotlar üzerinde denemeler gerçekleştirilmiştir. Hacimsel olarak fazla miktarda sperma (2-10 ml) tek seferde yönlü dondurma sayesinde saklanabilmektedir. Enkapsülasyon yönteminde hücrelerin etrafını saran jel benzeri bir yapı söz konusudur. Özellikle domuzlarda polispermiblok oluşmasını engellemek için bu yöntem kullanılmaktadır. Liyofilizasyon olarak adlandırılan saklama yöntemi ise bir kurutma işlemidir ve organizmalar, hücreler, dokular hatta bütün biyolojik ürünler bur grupta kendine yer bulabilmektedir. Türe özgü ya da nesli tükenmekte olan canlılar için tavsiye edilen sperma saklama yöntemleri geliştirilmiştir. Bütün saklama yöntemlerinin ortak dezavantajı ise sıvı ...
Bu çalışmada kısraklarda östrüsün; rektal palpasyon, ultrasonografi ve hızlı kan-progesteron testi yardımıyla karşılaştırmalı olarak tespiti amaçlandı. Materyal olarak yaşları 5-15 arasında değişen saf ve yarım kan 20 İngiliz kısrak... more
Bu çalışmada kısraklarda östrüsün; rektal palpasyon, ultrasonografi ve hızlı kan-progesteron testi yardımıyla karşılaştırmalı olarak tespiti amaçlandı. Materyal olarak yaşları 5-15 arasında değişen saf ve yarım kan 20 İngiliz kısrak kullanıldı. Östrüs tespiti, deneme aygırı muayenesinden sonra rektal palpasyon ve ultrasonografi yardımıyla gerçekleştirildi. Follikül çapları Östrüs boyunca rektal palpasyon ve ultrason kullanılarak östrüsün birinci gününden ovulasyon tespitine kadar gün aşırı ölçüldü. Follikül çapı ölçümlerini takiben kısraklardan gün aşırı olmak koşuluyla östrüsün birinci gününden ovulasyona kadar kan örnekleri alındı. Alınan örnekler 5000 devirde 20 dakika süreyle santrifüj edilerek serumlar elde edildi. Elde edilen serum iki eşit kısma ayrıldı. Birinci kısım serumda EIA (hızlı test), ikinci kusim serumda ise RIA yöntemine göre progesteron tayini yapıldı. Rektal palpasyon ve ultrasonografik ölçümler arasındaki korelasyon (r=0.92) önemli bulundu (p<0.01). Serum pro...
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AKOZ, MEHMET/0000-0001-5835-7115;WOS: 000278958100008The aim of this study was to determine the plasma beta-carotene, vitamin A and vitamin C levels and correlation between these parameters in cyclic and pregnant dairy cattle. A total of... more
AKOZ, MEHMET/0000-0001-5835-7115;WOS: 000278958100008The aim of this study was to determine the plasma beta-carotene, vitamin A and vitamin C levels and correlation between these parameters in cyclic and pregnant dairy cattle. A total of 101 Holstein cows used for this aim. Artificial inseminations (AI) were performed 12 h after determining the oestrus. The cows were allocated to two groups as pregnant (n=81) and nonpregnant (n=20) after the determination of pregnancy. Progesterone level was only higher on day 21 in pregnant cows than that in nonpregnant cows (P<0.05). There were variations in the mean levels of beta-carotene and vitamin A in pregnant and nonpregnant cows (P<0.05) whereas vitamin C levels in nonpregnant cows did not differ during the oestrus cycle. There was a negative correlation between the plasma levels of progesterone and beta-carotene (P<0.01) and progesterone and vitamin A (P<0.05) in pregnant cows, and a positive correlation between the plasma lev...
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Research Interests: Obstetrics, Biology, Fertility, Medicine, MISOPROSTOL, and 4 moreGynecology, Semen, Cervix, and Insemination
To compare the effect of ovsynch protocol beginning in luteal or follicular phases on pregnancy rates in cows and heifers, a total of 22 cows and 58 heif ers were allocated into two groups according to the ir s rum progesterone levels at... more
To compare the effect of ovsynch protocol beginning in luteal or follicular phases on pregnancy rates in cows and heifers, a total of 22 cows and 58 heif ers were allocated into two groups according to the ir s rum progesterone levels at the beginning of the ovsynch protocol. All animals were synchronized with i.m. injection of 50 μg lesirelin acetate on d 0 and 0.150 mg d-kloproste nol 7 d later. At the time of GnRH injection, anima ls with <1 ng/ml serum progesterone concentration were accepted as luteally not active (Group I, n=34, 7 cows and 27 heifers) while others with ≥1 ng/ml serum progesterone concentration were accep ted as luteally active (Group II, n=46, 15 cows and 31 heifers). Second dose of G nRH was injected 48 h after PGF 2α injections and fixed-time AI were performed 16 h after the second GnRH in all animals. Pregnancy diagnoses were performed on d 2 8 by ultrasonography with 7.5 MHz transrectal probe. The re was no difference between cows and heifers in pr egna...
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The object of this trial was to determine the efficacy of GnRH-PGF2α treatment to synchronize oestrus in sheep during the breeding season. A total of 30 ewes aging 18-24 months and 6 healthy rams aging 2-3 years were used in this... more
The object of this trial was to determine the efficacy of GnRH-PGF2α treatment to synchronize oestrus in sheep during the breeding season. A total of 30 ewes aging 18-24 months and 6 healthy rams aging 2-3 years were used in this experiment. Animals were divided into two groups. The first group received intramuscular (i.m.) injection of GnRH analogue (busereline) and 5 d later i.m. injection of PGF2α analogue (Triaprost tromethamine). The second group (control) was given twice, at 9 d interval, i.m. injection of PGF2α. Fourty-eight hours after the application of PGF2α, 400 IU of PMSG was injected i.m. to all the ewes. After the detection of oestrous, the ewes were hand-mated naturally. Pregnancy was determined using ultrasonography on d 30 after the mating. Multiple embryos were determined in each ewe using ultrasonography. Oestrus response, pregnancy, lambing rates, and litter sizes were 93.7%, 85.7%, 83.3% and 1.70; 86.6%, 84.6%, 81.8%, and 1.66 in the groups I and II, respectivel...
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Research Interests: Melatonin and Gynecology
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The effect of antifreeze protein type III (AFPIII) and human heat shock protein 70 (HHSP70), added to the vitrification medium of mouse embryos, on post-freeze/thaw in vitro embryonic growth rates and cell numbers were investigated. In... more
The effect of antifreeze protein type III (AFPIII) and human heat shock protein 70 (HHSP70), added to the vitrification medium of mouse embryos, on post-freeze/thaw in vitro embryonic growth rates and cell numbers were investigated. In total 20 female mice were synchronized. After synchronization, 2 females and 1 male were mated in the same cage. Twenty-four h after mating, the embryos were collected at the pronuclear stage. In total 8 groups were established, including a positive control group (C+), a negative control group (C-), and treatment groups that were vitrified in a medium added with 200, 400 and 800 ng/ml of AFPIII (AFPIII200, AFPIII400, AFPIII800), and 1, 2 and 4 µg/ml of HHSP70 (HHSP70-1, HHSP70-2, HHSP70-4). Solid surface vitrification (SSV) medium was prepared for the vitrification of the embryos. Once thawed, in vitro development rates of embryos were followed at 24, 48, 72 and 96 h. Four embryos, which progressed to the full blastocyst stage, were taken from each gr...
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... Par K. ÇOYAN, MB ATAMAN, H. ERDEM, A. KAYA et G. KASIKCI. ... Data were analyzed as the percentage of estrous and pre-gnancy with chi-square analysis to compare estrous and pre-gnancy rates between groups, and student&#x27;s-test... more
... Par K. ÇOYAN, MB ATAMAN, H. ERDEM, A. KAYA et G. KASIKCI. ... Data were analyzed as the percentage of estrous and pre-gnancy with chi-square analysis to compare estrous and pre-gnancy rates between groups, and student&#x27;s-test was perfor-med to compare average ...
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... Mesut KIRBAŞ*, Kenan ÇOYAN**, Bülent BÜLBÜL*, Mehmet Bozkurt ATAMAN**, Mehmet KÖSE*, Orhan AKMAN**, Şükrü DURSUN* Geliş Tarihi: 02.03.2009 Kabul Tarih: 03.04.2009 ... Materyal - Metot Çalışmada Brown Swiss ırkı 22 baş inek ve 58 baş... more
... Mesut KIRBAŞ*, Kenan ÇOYAN**, Bülent BÜLBÜL*, Mehmet Bozkurt ATAMAN**, Mehmet KÖSE*, Orhan AKMAN**, Şükrü DURSUN* Geliş Tarihi: 02.03.2009 Kabul Tarih: 03.04.2009 ... Materyal - Metot Çalışmada Brown Swiss ırkı 22 baş inek ve 58 baş düve kullanıldı. ...
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Öztürk, Caner (Aksaray, Yazar)The present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters.... more
Öztürk, Caner (Aksaray, Yazar)The present study was conducted to examine the protective roles of cysteamine, trehalose, alpha-lipoic acid and combinations of these antioxidants on post-thawed bull sperm and oxidative stress parameters. Five healthy Holstein bull (3-4 years old) were used. Eight ejaculates for each bull were collected and pooled. Pooled ejaculate, splitted into seven equal aliquots and diluted at 37 °C with base extenders containing cysteamine 2 mM, trehalose 50 mM, alpha-lipoic acid (ALA) 1 mM, cysteamine 2 mM + trehalose 50 mM, ALA 1 mM + trehalose 50 mM, cysteamine 2 mM + ALA 1 mM and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The combination of cysteamine 2 mM and ALA 1 mM of the semen extender improved the percentages of post-thawed subjective motility (68 ± 2.7%), and progressive motility (42.9 ± 4.7%), compared with the controls (61 ± 4.2% and 37.5 ± 8%, respectively, non- significant...
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Determination of the optimal PMSG dose for inducing increased the prolificacy in Akkaraman cross-bred ewes synchronized with the different doses of fluorogestone acetate (FGA) outside the breeding season was the aim of this study. A total... more
Determination of the optimal PMSG dose for inducing increased the prolificacy in Akkaraman cross-bred ewes synchronized with the different doses of fluorogestone acetate (FGA) outside the breeding season was the aim of this study. A total of 90 non-lactating ewes were ...
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Bu calismada, guvercin yumurta sarisiyla hazirlanmis koc spermasi sulandiricilarina eklenen metiyonin ve trehalozun dondurma sonrasi spermatolojik ve biyokimyasal parametreler uzerine etkilerinin arastirilmasi amaclandi. Arastirmada 3 bas... more
Bu calismada, guvercin yumurta sarisiyla hazirlanmis koc spermasi sulandiricilarina eklenen metiyonin ve trehalozun dondurma sonrasi spermatolojik ve biyokimyasal parametreler uzerine etkilerinin arastirilmasi amaclandi. Arastirmada 3 bas ergin (1-2 yas) Konya Merinosu irki koclara ait ejakulatlar kullanildi. Miks yapilan ejakulatlar 3 esit hacme bolunerek guvercin yumurtasi (kontrol), guvercin yumurtasi + 2 mM metiyonin ve guvercin yumurtasi + 25 mM trehaloz iceren Tris sulandiricisiyla ml’de yaklasik 4 x 108 spermatozoa olacak sekilde 37 oC’ta sulandirildi ve donduruldu. Dondurma-cozdurme sonrasi metiyonin iceren sulandirici grubunda LPO duzeyleri (103.6±12.6 µmol) trehaloz iceren gruba (66.2±9.5 µmol) gore daha yuksek (p 0.05). GPx aktivitesi ve GSH duzeyleri yonunden incelendiginde, farkli sulandiricilarla sulandirilmis sperma gruplari arasinda onemli bir fark gozlenmedi. Metiyonin iceren sulandirici grubunda akrozom butunlugu (%50.0±3.0) kontrol grubuna gore (%36.7±3.2) daha yu...
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Özet: Bu çalışmada, saha şartlarındaki ineklerde PGFz<ı + PGFZft' GnRH + PGFzw PRID + PGFzu ve deri altı implant + PGFzu uygulamalarının östrüs senkronizasyonu ve gebelik oranları üzerine etkisi araştırıldı. Materyalolarak 125 baş... more
Özet: Bu çalışmada, saha şartlarındaki ineklerde PGFz<ı + PGFZft' GnRH + PGFzw PRID + PGFzu ve deri altı implant + PGFzu uygulamalarının östrüs senkronizasyonu ve gebelik oranları üzerine etkisi araştırıldı. Materyalolarak 125 baş Holstein ırkı inek kullanıldı. inekler tesadüfi örnekleme yöntemi ile beş gruba ayrıldı. Birinci gruba (n= 25) 11 gün arayla iki kez PGFZa enjekte edildi. ikinci gruba (n= 25) O. gün GnRH ve 7. gün PGF1u enjeksiyonu yapıldı. Üçüncü gruptaki ineklere (n= 25) 12 gün süreyle PRID ve PRID'in uzaklaştırılmasından bir gün önce PGF2a uygulandı. Dördüncü gruptaki ineklere (n=25) ise kulak derisi altına yerleştirilen progesteron içeren implantlar 9 gün süreyle tutuldu ve implant uzaklaştırılmadan bir gün önce PGFzcı enjekte edild i. Beşinci gruptaki inekler (n=25) kontrol grubunu oluşturdu. Tohumlamalar i. grupta son uygulamadan 72 ve 96, II, III ve iV. gruplarda ise 48 ve 72 saat sonra iki kez yapıldı. Kontrol grubundaki inekler (n= 25) ise gelişen östr...
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Ineklerde ostrus senkronizasyonu icin kullanilan ovsynch protokolunun siklusun luteal veya follikuler donemlerinde baslamasinin gebelik oranlarina etkisinin arastirilmasi amaciyla, 22 inek ve 58 duve, uygulamanin baslangicindaki serum... more
Ineklerde ostrus senkronizasyonu icin kullanilan ovsynch protokolunun siklusun luteal veya follikuler donemlerinde baslamasinin gebelik oranlarina etkisinin arastirilmasi amaciyla, 22 inek ve 58 duve, uygulamanin baslangicindaki serum progesteron duzeylerine gore iki gruba ayrildi. Butun hayvanlar 0. gun 50 µg lesirelin acetate ve bundan 7 gun sonra 0.150 mg d-kloprostenol enjeksiyonu ile senkronize edildi. GnRH enjeksiyonu sirasinda serum progesterone seviyesi
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Precise estimation of ram fertility is important for sheep farming to sustain reproduction efficiency and profitability of production. There, however, is no conventional method to accurately predict ram fertility. The objective of this... more
Precise estimation of ram fertility is important for sheep farming to sustain reproduction efficiency and profitability of production. There, however, is no conventional method to accurately predict ram fertility. The objective of this study, therefore, was to ascertain proteomic profiles of ram sperm having contrasting fertility phenotypes. Mature rams (n = 66) having greater pregnancy rates than average (89.4 ± 7.2%) were assigned into relatively-greater fertility (GF; n = 31; 94.5 ± 2.8%) whereas those with less-than-average pregnancy rates were assigned into a lesser-fertility (LF; n = 25; 83.1 ± 5.73%; P = 0.028) group. Sperm samples from the outlier greatest- and least-fertility rams (n = 6, pregnancy rate; 98.4 ± 1.8% and 76.1 ± 3.9%) were used for proteomics assessments utilizing Label-free LC-MS/MS. A total of 997 proteins were identified, and among these, 840 were shared by both groups, and 57 and 93 were unique to GF and LF, respectively. Furthermore, 190 differentially abundant proteins were identified; the abundance of 124 was larger in GF while 66 was larger in LF rams. The GF ram sperm had 79 GO/pathway terms in ten major biological networks while there were 47 GO/pathway terms in six biological networks in sperm of LF rams. Accordingly, differential abundances of sperm proteins between sperm of GF and LF rams were indicative of functional implications of sperm proteome on male fertility. The results of this study emphasize there are potential protein markers for evaluation of semen quality and estimation of ram sperm fertilizing capacity.
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Objective: To examine the effects of paclitaxel and resveratrol on rabbit semen. Methods: This study consisted of four groups: control group (40 mL saline), paclitaxel group (5 mg/kg paclitaxel), resveratrol group (4 mg/kg resveratrol)... more
Objective: To examine the effects of paclitaxel and resveratrol on rabbit semen. Methods: This study consisted of four groups: control group (40 mL saline), paclitaxel group (5 mg/kg paclitaxel), resveratrol group (4 mg/kg resveratrol) and paclitaxel+resveratrol group (5 mg/kg paclitaxel+4 mg/kg resveratrol). Administrations were i.v. (in 40 mL saline) and continued 8 weeks. Sperm motility was evaluated using phase-contrast microscopy. Mitochondrial activity, membrane and acrosome integrity were performed by fluorescence staining. Lipid peroxidation, total glutathione and antioxidant potential levels were determined by spectrophotometry. Results: Paclitaxel decreased the sperm motility and fluorescence staining results compared to the control (P<0.05). The paclitaxel and resveratrol group showed better results of the same parameters compared to the paclitaxel group (P<0.05). No significant difference was observed in lipid peroxidation, total glutathione, antioxidant potential and fertility results (P>0.05). Results of this study showed that paclitaxel decreased semen parameters and resveratrol had a protective effect on these parameters. Conclusions: Paclitaxel has negative effects on spermatological indicators and biochemical assays, while resveratrol prevents these negative effects of paclitaxel.
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In this study, the efficacy of human chorionic gonadotropin (hCG) and gonadotropin releasing hormone (GnRH) administered to advance the ovulation in oestrous in mares was examined. Thoroughbred mares (36) were divided into 3 groups. After... more
In this study, the efficacy of human chorionic gonadotropin (hCG) and gonadotropin releasing hormone (GnRH) administered to advance the ovulation in oestrous in mares was examined. Thoroughbred mares (36) were divided into 3 groups. After the detection of (35 mm follicle in diameter, with the use of ultrasound, 3000 IU ho, 40mg btlsereline, GnRH and 2 ml placebo were injected to the mares in groups 1, 2 and 3 respectively. Follicles were monitored every 12 hr with ultrasonography until the occurrence of ovulation. The mares were mated naturally every other day by the end of oestrus. Pregnant mares were determined using B-mode real time ultrasound on day 20 after last mating. There was a statistical significance of(P<0.01) between groups 1 and 3, and also 2 and 3 on the average ovulation time and numbers of mating required per conception. On the other hand, the differences of the pregnancy rates among the groups were not significant. In conclusion, hCG and GnRH could be used for inducing ovulation in cyclic mares.
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The aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable... more
The aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable reproductive disorders were used. Cloprostenol, a synthetic analogue of PGF 2α, was administered intramuscularly twice (500 μg) 11-days apart. After second cloprostenol administration, animals were observed for the overt signs oestrus and, inseminated twice with frozen semen after confirmation of oestrus by rectal palpation. Electrical conductivity of vaginal mucosa was measured through ventral wall of vagina adjacent to caudal end of cervix uteri by a handy-held instrument. Vaginal conductance of animals was measured twice, with a 12 h interval, on days 0 (day of oestrus), 3, 6, 10, 13, 19, 20 and 21 in cows, and on days 0 (day of oestrus), 3, 6, 10, 13, 18, 19 and 20 in heifers. To estimate plasma progesterone levels, jugular blood samples (10 mL) were also...
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We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots... more
We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 °C with base extenders containing lycopene (1 × 10(-3) g ml(-1) ) and resveratrol (1 mm), and no antioxidant (control), was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 ± 3.8 and 61.9 ± 4.0%) and progressive motility (38 ± 2.4 and 37 ± 8.8), compared with the controls (50.7 ± 2.65 and 33.3 ± 3.74%, respectively, P < 0.05). Resveratrol provided a higher ALH (4.3 ± 0.1), in comparison with the control (3.9 ± 0.3, P < 0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitoc...
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This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical... more
This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 μg/kg/day, and glucomannan combined with AF. The animals in all groups were treated for 12 weeks by the above-mentioned diet. When compared to control, AF-treatment caused significant decrease in alpha-naphthyl acetate esterase- (ANAE-) positive peripheral blood lymphocyte (PBL) percentages. The addition of the glucomannan to AFcontaining diet recovered the adverse effects of AF on sacculus rotundus and increased the ANAE-positive PBL counts. These results suggested that glucomannan was effective against the negative effects of AF in rabbits.