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Dendrimer nanocomposites (DNC) are hybrid nanoparticles formed by the dispersion and immobilization of guest atoms or small clusters in dendritic polymer matrices. They have a great potential in biomedical applications due to their... more
Dendrimer nanocomposites (DNC) are hybrid nanoparticles formed by the dispersion and immobilization of guest atoms or small clusters in dendritic polymer matrices. They have a great potential in biomedical applications due to their controlled composition, predetermined size, shape and variable surface functionalities. In this work, d=5–25?nm spherical nanoparticles composed of gold and poly(amidoamine) (PAMAM) dendrimers have been selected to demonstrate
Research Interests: Materials Engineering, Materials Science, Chemistry, Inorganic Chemistry, Physical Chemistry, and 15 moreHealth Sciences, Imaging, Science, Nanoparticle, Drug delivery, Nanocomposites, Nanotechnology, Biological Chemistry, Dendrimers, Polymer, Nanocomposite, Dendrimer, Biomedical Application, Colloidal Gold, and Gene Transfer
Research Interests: Engineering, Materials Science, Optics, Femtosecond Laser, Confocal Microscopy, and 14 moreCrystals, Microscope, Luminescence, Laser Scanning Confocal Microscopy, Photonic Crystal Fibers, Optical fiber, Next Generation, Fiber Optic, Photonic crystal fiber, Indexation, Single Mode Fiber, Collection Efficiency, Optical Fibers, and Field of View
Research Interests: Chemistry, Analytical Chemistry, Capillary electrophoresis, Nanotechnology, Medicine, and 15 moreDendrimers, Amines, Functionalization, Nanostructures, Acknowledgement, The, Gel Permeation Chromatography, Analyst, Drug Carriers, Dendrimer, Amine, Molecular Structure, Permission, acetamide, and Dispersity
Research Interests: Chemistry, Analytical Chemistry, Chromatography, Medicine, Dendrimers, and 10 morePolyamines, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Poly(amidoamine), Analyst, Conjugate, Biocompatible Materials, Dendrimer, and Sodium Dodecyl Sulfate
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Research Interests: Engineering, Chemistry, Mass Spectrometry, Nuclear Magnetic Resonance, NMR Spectroscopy, and 15 moreCapillary electrophoresis, DISTRIBUTION, Polymer, Surface modification, Gel Permeation Chromatography, CHEMICAL SCIENCES, Genetic analysis for qualitative and quantitative traits of crop plants, Distribution, Polyacrylamide Gel Electrophoresis, Potentiometric Titration, Dendrimer, Distribution Center, Electrophoretic Mobility, Electrospray Ionization, and Ethylenediamine
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Research Interests: Chemistry, Analytical Chemistry, Chromatography, Drug delivery, Nanotechnology, and 15 moreFolic acid, Medicine, Dendrimers, Quality Control, Polyamines, HPLC, Folate Receptor, Drug Delivery Systems, High Performance Liquid Chromatography, High Pressure Liquid Chromatography, Methotrexate, Dendrimer, Physicochemical Properties, Biochemistry and cell biology, and Pharmacology and pharmaceutical sciences
Research Interests: Materials Science, Modeling, Chemical Physics, Comparative Study, Atomic Force Microscopy, and 15 moreMedicine, Dendrimers, Molecular Dynamics, Elasticity, Computer Simulation, Mathematical Sciences, Mica, Biomimetic materials, Molecular Conformation, Materials Testing, Aqueous Solution, Biocompatible Materials, Dendrimer, Experimental Data, and Molecular Dynamic Simulation
Research Interests: Engineering, Chemical Engineering, Chemistry, Analytical Chemistry, Health Sciences, and 15 moreScience, Capillary electrophoresis, Materials Science and Engineering, Medicine, Dendrimers, Biochemistry and Biotechnology, Electrophoresis, Acetylation, Molecular weight, Charge Density, Dendrimer, Size Exclusion Chromatography, Biochemistry and cell biology, Molecular Structure, and Electrophoretic Mobility
Research Interests: Engineering, Chemical Engineering, Chemistry, Analytical Chemistry, Health Sciences, and 14 morePolymer Chemistry, Science, Capillary electrophoresis, Materials Science and Engineering, Medicine, Dendrimers, Biochemistry and Biotechnology, Electrophoresis, Polyvinyl alcohol, Poly Vinyl Alcohol, Poly(amidoamine), Reproducibility of Results, Dendrimer, and Biochemistry and cell biology
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Research Interests: Flow Cytometry, Fluorescence, Biology, Biophysical Chemistry, Folic acid, and 15 moreMedicine, Biological Sciences, Humans, Mice, Animals, Green Fluorescent Protein, CHEMICAL SCIENCES, Molecular cloning, In Vivo, Optical fiber, Photons, Fluorescent probes, Drug Therapy, Minimally Invasive, and fluorescent dyes
Research Interests: Chemistry, Organic Chemistry, Kinetics, Magnetic Resonance Spectroscopy, Medicine, and 15 moreCell line, In Vitro, Humans, Mice, Animals, Neurons, Esterase, Morphine, High Pressure Liquid Chromatography, Guinea Pig, Bioorganic and medicinal Chemistry, Biological activity, Hydrolysis, Guinea pigs, and Pharmacology and pharmaceutical sciences
Research Interests: Engineering, Chemistry, Flow Cytometry, Folic acid, Medicine, and 15 moreDendrimers, Biological Sciences, Humans, Cancer Therapy, Drug Design, CHEMICAL SCIENCES, KB cells, Molecular Conformation, Molecular weight, Cell Proliferation, Conjugate, Drug Carriers, Dendrimer, Antineoplastic Agents, and Biomacromolecules
Research Interests: Materials Engineering, Chemistry, Organic Chemistry, Magnetic Resonance Spectroscopy, Medicine, and 13 moreDNA, Combinatorial Chemistry, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Bioconjugate Chemistry, Molecular weight, Conjugate, Thiol, Dendrimer, Oligonucleotides, Biochemistry and cell biology, single stranded DNA, Oligonucleotide, and coloring agents
We utilize a real-time acoustic technique, based on pulse-echo measurements to detect formation of microbubbles in an aqueous solution of a silver/dendrimer nanocomposite (DNC). Wave-field plots of successive recordings illustrate the... more
We utilize a real-time acoustic technique, based on pulse-echo measurements to detect formation of microbubbles in an aqueous solution of a silver/dendrimer nanocomposite (DNC). Wave-field plots of successive recordings illustrate the generation and behavior of bubbles created by the optical breakdown process. A significant threshold reduction is achieved with DNC particles compared to its host dendrimer, enabling a diverse field of low-threshold breakdown applications.
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Respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (CF). Species within the Burkholderia cepacia... more
Respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (CF). Species within the Burkholderia cepacia complex are especially problematic in this patient population. We investigated a novel surfactant-stabilized oil-in-water nanoemulsion (NB-401) for activity against 150 bacterial isolates recovered primarily from CF respiratory tract specimens. These specimens included 75 Burkholderia isolates and 75 isolates belonging to other CF-relevant species including Pseudomonas , Achromobacter , Pandoraea , Ralstonia , Stenotrophomonas , and Acinetobacter . Nearly one-third of the isolates were multidrug resistant, and 20 (13%) were panresistant based on standard antibiotic testing. All isolates belonging to the same species were genotyped to ensure that each isolate was a distinct strain. The MIC 90 of NB-401 was 125 μg/ml. We found no decrease in activity again...
Research Interests: Microbiology, Biofilms, Cystic Fibrosis, Medical Microbiology, Biology, and 15 moreMedicine, Humans, Multidrug Resistance, Antimicrobial, Burkholderia, Emulsions, Microbial Sensitivity Tests, Health risk, Ciprofloxacin, Burkholderia Cepacia Complex, Multiple Drug Resistance, Achromobacter, Chronic Infection, Cause of death, and Antimicrobial agent
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Research Interests: Materials Science, Magnetic Resonance Imaging, Fluorescence, Nanomedicine, Crystallization, and 15 moreFolic acid, Medicine, Multidisciplinary, Humans, Squamous Cell Carcinoma, Nanostructures, Iron Oxide, Magnetics, Particle Size, Molecular Conformation, Materials Testing, Drug Carriers, Dendrimer, Ferric Compounds, and Contrast Media
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Research Interests: Engineering, Materials Engineering, Materials Science, Health Sciences, Pharmacy, and 12 moreBiomedical Engineering, Cancer, Imaging, Science, Circuits and Systems, Drug delivery, Nanotechnology, Biological Chemistry, Biomedical, Electronic Engineering and Computer Science, Characterization and Evaluation of Materials, and High energy
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Research Interests: Immune response, Flow Cytometry, Biology, Cytokines, Cell Division, and 15 moreInfluenza virus, Biological Sciences, influenza A, Female, Animals, Immunization, Immune system, Antibody, Emulsions, influenza A virus, Influenza Vaccine, Adjuvant, immunoglobulin G, Immunoglobulin A, and Influenza vaccines
Purpose. We sought to develop nanoscale drug delivery materials that would allow targeted intracellular delivery while having an imaging capability for tracking uptake of the material. A complex nanodevice was designed and synthesized... more
Purpose. We sought to develop nanoscale drug delivery materials that would allow targeted intracellular delivery while having an imaging capability for tracking uptake of the material. A complex nanodevice was designed and synthesized that targets tumor cells through the folate receptor. Methods. The device is based on an ethylenediamine core polyamidoamine dendrimer of generation 5. Folic acid, fluorescein, and methotrexate
Research Interests: Chemistry, Health Sciences, Biomedicine, Biomedical Engineering, Medical Law, and 15 moreDrug delivery, Folic acid, Medicine, Molecular modeling, Internalization, In Vitro, Humans, Drug Design, Folate Receptor, Drug Delivery Systems, Methotrexate, Drug Carriers, Dendrimer, Internalisation, and Intracellular
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Starburst polyamidoamine dendrimers are a new class of synthetic polymers with unique structural and physical characteristics. These polymers were investigated for the ability to bind DNA and enhance DNA transfer and expression in a... more
Starburst polyamidoamine dendrimers are a new class of synthetic polymers with unique structural and physical characteristics. These polymers were investigated for the ability to bind DNA and enhance DNA transfer and expression in a variety of mammalian cell lines. Twenty different types of polyamidoamine dendrimers were synthesized, and the polymer structure was confirmed using well-defined analytical techniques. The efficiency of plasmid DNA transfection using dendrimers was examined using two reporter gene systems: firefly luciferase and bacterial beta-galactosidase. The transfections were performed using various dendrimers, and levels of expression of the reporter protein were determined. Highly efficient transfection of a broad range of eukaryotic cells and cell lines was achieved with minimal cytotoxicity using the DNA/dendrimer complexes. However, the ability to transfect cells was restricted to certain types of dendrimers and in some situations required the presence of addit...
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Research Interests: Immunology, Cytokines, Nanoparticles, Medicine, Multidisciplinary, and 15 moreMice, Immunity, Animals, Immunization, Lung, Respiratory Syncytial Virus, PLoS one, Human Respiratory Syncytial Viruses, Mouse Model, Immune system, Emulsions, Bronchiolitis, Intranasal Administration, Eosinophilia, and Inactivated vaccine
Research Interests: Molecular Biology, Electrochemistry, Biology, Medicine, Gene expression, and 15 moreCell Culture, Biological Sciences, Cell line, Environmental Sciences, Mice, Animals, Luciferase, Delivery System, Base Sequence, Drug Carriers, Dendrimer, Drug Stability, Gene Expression Regulation, High density, and Electrostatic interaction
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Traumatic dental injuries are often irreversible, underscoring the need for therapies that protect dental pulp cells and enhance their regeneration. We hypothesized that generation 5 poly amido amine (PAMAM) dendrimers (G5),... more
Traumatic dental injuries are often irreversible, underscoring the need for therapies that protect dental pulp cells and enhance their regeneration. We hypothesized that generation 5 poly amido amine (PAMAM) dendrimers (G5), functionalized with fluorescein isothiocyanate (FL) and αVβ3-specific, cyclic arginine-glycine-aspartic acid (RGD) peptides, will bind to dental pulp cells (DPCs) and modulate their differentiation. Dental pulp cells and mouse odontoblast-like cells (MDPC-23) (±) treated with G5-FL-RGD were analyzed via Western blot, RT-PCR, and quantitative PCR. Transcription of dental differentiation markers was as follows: Dentin matrix protein (DMP-1), dentin sialoprotein (DSPP), and matrix extracellular phosphoglycoprotein (MEPE) as well as vascular endothelial growth factor (VEGF) all increased via the JNK pathway. Long-term G5-RGD treatment of dental pulp cells resulted in enhanced mineralization as examined via Von Kossa assay, suggesting that PAMAM dendrimers conjugated...
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Research Interests: Materials Science, Biomedical Engineering, Fluorescence, Nanoparticles, Medicine, and 15 moreDendrimers, Biomedical, Fiber, Biomedical Optics, Humans, Mice, Animals, Luminescence, Drug Delivery Systems, KB cells, Fiber Optic Technology, Dendrimer, Equipment Design, Equipment Failure Analysis, and fluorescent dyes
Research Interests: Chemistry, Health Sciences, Molecular Biology, Biology, Medicine, and 15 moreDendrimers, Gene expression, Medical Biotechnology, Mice, Animals, Gene transfer techniques, Clinical Sciences, Myocardium, Model System, Heart Transplantation, Dendrimer, Gene Transfer, Interleukin, Immunosuppressive Agents, and Human gene
Research Interests: Biophysics, Chemistry, Organic Chemistry, Flow Cytometry, Drug delivery, and 15 moreNanoparticles, Nanotechnology, Folic acid, Medicine, Dendrimers, Humans, Endocytosis, Drug Delivery Systems, Chemistry Biology, Protein Binding, Drug Carriers, Dendrimer, Antineoplastic Agents, Biochemistry and cell biology, and avidity
Research Interests: Organic Chemistry, Biology, Folic acid, Medicine, Polyamines, and 15 moreDNA, Internalization, Humans, Drug Design, Folate Receptor, Phosphates, Molecular weight, Chemistry Biology, Structure activity Relationship, Dendrimer, Oligonucleotides, Biochemistry and cell biology, Molecular Structure, Fluorescein, and Oligonucleotide
Prior studies suggested that nanoparticle drug delivery might improve the therapeutic response to anticancer drugs and allow the simultaneous monitoring of drug uptake by tumors. We employed modified PAMAM dendritic polymers <5 nm in... more
Prior studies suggested that nanoparticle drug delivery might improve the therapeutic response to anticancer drugs and allow the simultaneous monitoring of drug uptake by tumors. We employed modified PAMAM dendritic polymers <5 nm in diameter as carriers. Acetylated dendrimers were conjugated to folic acid as a targeting agent and then coupled to either methotrexate or tritium and either fluorescein or 6-carboxytetramethylrhodamine. These conjugates were injected i.v. into immunodeficient mice bearing human KB tumors that overexpress the folic acid receptor. In contrast to nontargeted polymer, folate-conjugated nanoparticles concentrated in the tumor and liver tissue over 4 days after administration. The tumor tissue localization of the folate-targeted polymer could be attenuated by prior i.v. injection of free folic acid. Confocal microscopy confirmed the internalization of the drug conjugates into the tumor cells. Targeting methotrexate increased its antitumor activity and mark...