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We evaluated the performance of a third-generation fluorescence enzyme immunoassay kit (FEIA; Tosoh Bioscience) for progesterone (P4) measurement in canine serum to identify the day of luteinizing hormone (LH) peak and ovulation in... more
We evaluated the performance of a third-generation fluorescence enzyme immunoassay kit (FEIA; Tosoh Bioscience) for progesterone (P4) measurement in canine serum to identify the day of luteinizing hormone (LH) peak and ovulation in bitches. We conducted P4 assays on 54 serum samples using a FEIA and a chemiluminescence immunoassay (CLIA; Siemens). For the FEIA kit, the linearity test, recovery test, inter- and intra-assay CVs, and total error observed (TEo) were calculated. Serum samples from 28 bitches were used to evaluate the association of P4 concentration with the day of LH peak and the day of ovulation based on P4 thresholds (P4 ≥ 2 ng/mL, and doubling at 4–8 ng/mL in the following 48 h), and with pregnancy length. Linearity was 75–97% and 86–94% for high (37.0 ng/mL) and medium (3.8 ng/mL) pool serum samples, respectively. Recovery was 86.4–119%. Intra-assay CVs were 2.6%, 3.3%, and 5.2% for low (0.23 ng/mL), medium (6.24 ng/mL), and high (38.3 ng/mL) pool concentrations. Inter-assay CVs were 2.22% and 2.53% for P4 concentrations of 2.70 ng/mL and 8.2 ng/mL, respectively. TEo was 8.72% and 22.7% for P4 concentrations of 2 and 8 ng/mL, respectively. The mean pregnancy length from the day of LH peak and ovulation were 64.6 ± 1.7 and 63.3 ± 1.1 d, respectively. The third-generation FEIA kit that we tested was highly reliable.
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The angiotensin-converting enzyme (ACE) inhibitory activity of water-soluble extracts (WSE) from Asiago cheeses was assayed in two cheese production systems and with different ripening times. The WSE were ultrafiltered through 10 kDa and... more
The angiotensin-converting enzyme (ACE) inhibitory activity of water-soluble extracts (WSE) from Asiago cheeses was assayed in two cheese production systems and with different ripening times. The WSE were ultrafiltered through 10 kDa and 3 kDa cut-off membranes to evaluate the ACE inhibitory activity of long and short peptides, respectively. The cheese production systems had no significant effect on the ACE inhibitory activity, whereas
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This study aimed at evaluating the relationship between biomarkers of oxidative stress (OS) in seminal plasma and sperm motility in bulls before and after cryopreservation. Three ejaculates per bull were collected from 20 young bulls.... more
This study aimed at evaluating the relationship between biomarkers of oxidative stress (OS) in seminal plasma and sperm motility in bulls before and after cryopreservation. Three ejaculates per bull were collected from 20 young bulls. Each ejaculate was analyzed for motility before and after cryopreservation (by CASA), and the SP concentration of Advanced Oxidation Protein Products (AOPP), thiols, and carbonyl groups (CT) were examined. Then, based on their motility, the ejaculates were grouped into: high motility fresh (HMF), low motility fresh (LMF), high motility thawed (HMT), and low motility thawed (LMT) groups. Higher AOPP and thiol concentrations on SP were related (p < 0.05) to the higher LIN and BCF and lower ALH of fresh semen. In addition, AOPP and thiols were significantly higher in HMF than LMF. As a confirmation of this, the Receiver Operating Characteristic (ROC) curve analysis showed that AOPP and thiol concentrations in SP were able to discriminate between HMF an...
Research Interests: Andrology, Oxidative Stress, Cryopreservation, Motility, Sperm, and 3 moreAnimals, Semen, and Sperm Motility
Abstract Horse practitioners have difficulties with undesirable behaviors exhibited by their horses (Equus caballus), such as cribbing, weaving, or head shaking. It has been suggested that most of behavioral disorders originate from the... more
Abstract Horse practitioners have difficulties with undesirable behaviors exhibited by their horses (Equus caballus), such as cribbing, weaving, or head shaking. It has been suggested that most of behavioral disorders originate from the animals’ attempt to cope with a suboptimal environment. To evaluate animal welfare, it is important to consider both behavioral and physiological parameters. Studies on animal welfare underline the importance of the monitoring of hormonal concentrations, particularly hormones that indicate stress, such as cortisol. The aims of this research were to compare cortisol and dehydroepiandrosterone (DHEA) concentrations of 14 healthy horses (HH) and 40 horses displaying behavioral pathologies (BPHs), to calculate the cortisol-DHEA ratio as marker of chronic stress and to find a relationship between aspects of horses management and the presence of BPHs. The methodology of radio-immuno-assay was used, to determine cortisol and DHEA levels in blood and hair. A questionnaire was completed by horse owners or barn managers and used to investigate the different characteristics of boarding barns, where the horses were housed. BPH horses registered a significantly (P
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Omnigen-AF (OAF) increases leukocyte functions in immunosuppressed animal models and reduces incidence of infectious diseases in early lactating dairy cows, although its mode of action is still unclear. This study aims to provide a wider... more
Omnigen-AF (OAF) increases leukocyte functions in immunosuppressed animal models and reduces incidence of infectious diseases in early lactating dairy cows, although its mode of action is still unclear. This study aims to provide a wider perspective of the metabolic effect of OAF to test its potential as a strategy to address metabolic disorders of the transition period. A group of 10 Holstein dairy cows were divided into 2 groups: The treated group (IMS; 5 cows) received 32.5 g of OAF twice a day (65 g d−1) as top-dress in the morning and afternoon feeds from −55 to 42 days from calving (DFC), whereas the control group (CTR; 5 cows) received no supplementation. From −62 to 42 DFC, body condition score, body weight, dry matter intake, rumination time and milk yield were measured; blood samples were collected weekly to assess a wide hematochemical profile and to test white blood cell functions by ex-vivo challenge assays. At 30 DFC, rumen fluid was collected and analyzed for pH, vola...
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... Regazzo, Daniela (2005) Caratterizzazione di peptidi ace-inibitori prodotti ad opera di batteri proteolitici impiegati per la realizzazione di latte fermentati ... EPrints Logo. Padua@Thesis is powered by EPrints 3 which is developed... more
... Regazzo, Daniela (2005) Caratterizzazione di peptidi ace-inibitori prodotti ad opera di batteri proteolitici impiegati per la realizzazione di latte fermentati ... EPrints Logo. Padua@Thesis is powered by EPrints 3 which is developed by the School of Electronics and Computer Science ...
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The hypothalamus-pituitary–adrenal axis response to a challenge was proposed for genetic selection of robust and resilient animals. As ACTH (adrenocorticotropic hormone) test and hormone measurements in blood may result impractical, it... more
The hypothalamus-pituitary–adrenal axis response to a challenge was proposed for genetic selection of robust and resilient animals. As ACTH (adrenocorticotropic hormone) test and hormone measurements in blood may result impractical, it may be useful to measure salivary hormones in response to natural stressors, after an accurate biological validation, to control factors that could contribute to the response. We evaluated whether animal handling during performance test affects salivary HC and DHEA secretion and could be used for selection. We tested the effects of habituation to repeated handling and THI as putative bias. Bull calves (N = 273) undergoing performance test were sampled at 8–9 and 11–13 months (N = 101), 8–9 months (N = 131), or 11–13 months (N = 41). On each test day (D0), calves were isolated, conducted to a squeeze chute and immobilized for 6 min. Saliva samples were collected in the morning after feed administration (T0), and after 6 min immobilization in the squeez...
Research Interests: Engineering, Chemistry, Food Science, Dairy Science, Animal Production, and 15 moreBiological Sciences, Functional Foods, Antioxidants, Cardiovascular Pharmacology, Hydrolysate, Dairy, Enzyme, Dairy Technology, CHEMICAL SCIENCES, Fermentation, Bioactive Peptides, Food Sciences, Heat Shock Proteins, Angiotensin Converting Enzyme, and Enzymatic hydrolysis
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High neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular,... more
High neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular, advanced oxidation protein products (AOPP), are OS biomarkers linked to PMN activity. To test this hypothesis, the relationship between protein oxidation and uterus health was studied in thirty-eight dairy cows during the puerperium. The animals were found to be cycling, without any signs of disease and pharmacological treatments. PMN count was performed either through a cytobrush or a uterine horn lavage (UHL). Cows were classified into four groups, based on the uterine ultrasonographic characteristics and the PMN percentage in the uterine horns with a higher percentage of high neutrophil horn (HNH). They were classified as: Healthy (H); Subclinical Endometritis (SCE); Grade 1 Endometritis (EM1); and Grade 2 Endometritis (EM2). AOPP and carbonyls wer...
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The relationship between dietary variations and oxidative stress has been poorly investigated under field conditions. Changes of the diet starch content were introduced to study whether they could induce oxidative stress in early... more
The relationship between dietary variations and oxidative stress has been poorly investigated under field conditions. Changes of the diet starch content were introduced to study whether they could induce oxidative stress in early lactating heifers. In addition, the association between blood metabolic, endocrine responses and indicators of milk cellular immune functions have been investigated. Ten heifers were randomly assigned to two groups and fed with a basal total mixed ration until 34 days in milk (DIM). At 35 DIM control group continued to receive the same ration and the other group was allotted to experimental diets with low and high starch contents. The results of this study show that even a sudden change of diet is not associated with a consistent and significant change in most of the metabolic parameters considered. However, insulin and growth hormon (GH) levels were associated to a decreased probability of low leukocyte and neutrophil counts, but also to an increase in blood NAGase activity and milk lysozyme activity. These results suggest that GH induction and insulin release could have a selective influence on both number and activity of immune cells in blood and in milk.
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The aim of the study reported in this Research Communication was to analyse the variations of milk cortisol concentrations in response to the relocation of dairy cows between production groups. Milk cortisol measured during 3 consecutive... more
The aim of the study reported in this Research Communication was to analyse the variations of milk cortisol concentrations in response to the relocation of dairy cows between production groups. Milk cortisol measured during 3 consecutive days did not vary significantly in cows without environmental perturbation. However, relocation of cows caused a significant increase of cortisol in milk starting from the first milking after the group change. This suggests that cortisol in milk can be a suitable biomarker to assess the HPA response of dairy cows to a short/medium-term environmental challenge.
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Research Interests: Nutrition and Dietetics, Mass Spectrometry, Transport, Humans, Animals, and 14 moreMembrane transport proteins, Cattle, Intestinal absorption, Caco-2 cells, Digestive System, Time Factors, Food Sciences, Molecular Nutrition, Protein isoforms, Residue, Monolayer, Tight Junctions, Transcytosis, and microvilli
Research Interests: Physiology, Zoology, Melatonin, Brain, Female, and 8 moreAnimals, Male, Retina, Cetacea, Pineal Gland, Veterinary Sciences, Harderian Gland, and Tissue Fixation
The angiotensin-converting enzyme (ACE) inhibitory activity of water-soluble extracts (WSE) from Asiago cheeses was assayed in two cheese production systems and with different ripening times. The WSE were ultrafiltered through 10 kDa and... more
The angiotensin-converting enzyme (ACE) inhibitory activity of water-soluble extracts (WSE) from Asiago cheeses was assayed in two cheese production systems and with different ripening times. The WSE were ultrafiltered through 10 kDa and 3 kDa cut-off membranes to evaluate the ACE inhibitory activity of long and short peptides, respectively. The cheese production systems had no significant effect on the ACE inhibitory activity, whereas