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Growing energy needs and medium-term weakening of fossil energy reserves are driving forces towards the exploitation of alternative and renewable energy sources, such as biofuels from energy crops. In recent years, Camelina sativa (L.)... more
Growing energy needs and medium-term weakening of fossil energy reserves are driving forces towards the exploitation of alternative and renewable energy sources, such as biofuels from energy crops. In recent years, Camelina sativa (L.) Crantz has been rediscovered and is gaining popularity worldwide. The present work reports the results of a study on the life cycle, from cradle-to-gate, of C. sativa oil as a raw material for the production of biofuels in northern Italy, considering two scenarios, namely, the production of biodiesel (BD) and the extraction of pure vegetable oil (PVO). The functional unit was 1 megajoule of biofuel. A life cycle impact assessment (LCIA) was calculated according to the ILCD2011 procedure. Focusing on the global warming potential, the PVO scenario performs better than the BD scenario, with around 30 g CO2eq MJ−1. The net energy ratio (NER) exceeds unity for BD (approximately 1.4) or PVO (approximately 2.5). The same general trend was recorded for all ca...
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Although camelina [Camelina sativa (L.) Crantz] is a good source of protein, antioxidants, and polyunsaturated fatty acids, its antinutritional compounds limit its use in animal feeding. The aim of this study was to verify the effect of... more
Although camelina [Camelina sativa (L.) Crantz] is a good source of protein, antioxidants, and polyunsaturated fatty acids, its antinutritional compounds limit its use in animal feeding. The aim of this study was to verify the effect of feeding laying hens with up to 20% of camelina cake from a breeding line containing a low level of glucosinolates on performance, welfare, and eggshell quality. Two hundred and forty Hy-Line® hens from 18 to 51 weeks of age were divided into three treatments: control (C), camelina cake 10% (CAM10), and camelina cake 20% (CAM20). Egg number was recorded daily, while egg weight, feed consumption, and mortality were recorded weekly. At 24 and 43 weeks of hen age, shell resistance to fracture was measured. Our results demonstrate no detrimental effects for CAM10 and CAM20 diets on feed intake, growth performance, and welfare. No difference in egg production was detected among the diets. The significant (p < 0.05) interaction of diet and age factors su...
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Jatropha curcas L., a multipurpose shrub, originated in Central America, is present worldwide throughout tropical and subtropical regions. In India, J. curcas had recently been promoted as a potential source to reduce dependence on crude... more
Jatropha curcas L., a multipurpose shrub, originated in Central America, is present worldwide throughout tropical and subtropical regions. In India, J. curcas had recently been promoted as a potential source to reduce dependence on crude oil. However, our knowledge concerning genotype, phenotype and environmental interaction is limited. In the present study the magnitude of phenotypic growth, oil yield and quality of promising jatropha sources from India have been evaluated at Jorhat in Assam. The molecular basis of the phenotypic diversity present in different accessions predominantly recovered from different locations in India was also verified. After 36 months of field planting, significant differences were noticed among all accessions tested for agronomical and physiological parameters. Free fatty acids, triglyceride acid composition and the presence of phorbol esters and tocopherols have been studied as they influence oil quality. Integration of biochemical parameters with phys...
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ABSTRACT Recently, some species of the Brassicaceae family (Brassica napus, Camelina sativa and Brassica carinata) have become attractive for the exploitation of seed oil as fuel. In particular, Camelina sativa (CS) (chromosome number... more
ABSTRACT Recently, some species of the Brassicaceae family (Brassica napus, Camelina sativa and Brassica carinata) have become attractive for the exploitation of seed oil as fuel. In particular, Camelina sativa (CS) (chromosome number 2n=40, genome size 750 Mbp) is gaining interest in North America and Europe for biodiesel production. The extraction of oil from seeds makes protein-enriched flours (by-product) available as an ingredient in animal feed. However, exploitation of flours from the Brassicaceae may be limited by the presence of glucosinolates (GSLs). Chemically, they are β-thioglucoside N-hydroxysulfates with a side chain and sulfur linked β-D-glucopyranose. GSLs, after hydrolysis by the enzyme thioglucosidase (called myrosinase), produce different catabolites (e.g., isothiocyanates, thiocyanates, epithionitriles and nitriles) with detrimental and antinutritional characteristics. In CS, three main GSLs were identified and named GSL1 (glucoarabin 9-methyl-sulfinyl-nonyl-GSL) GSL2 (glucocamelinin 10-methyl-sulfynil-decyl-GSL) and GSL3 (11-methyl-sulfinyl-undecyl-GSL). GSL content in twelve CS genotypes was investigated by HPLC separation. Total GSL content ranged from 15.2 to 24.6 mmol Kg -1 dry weight (dw). Differences among genotypes were observed on the GSL pattern. In all genotypes, GSL2 represented the most abundant GSL being between 50% -60%. The content of the other two molecular species, GSL1 and GSL3, exhibited differences among genotypes. Six genotypes (Calena, Ligena, Cam120, Cam180, D11851 and FF006) showed levels of GSL1 higher than GSL3. The average content of GSL1 (3 mmol kg -1
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... Lima beans, Phaseolus lunatus (Fabaceae) LUCIA LIOI, CONCETTA LOTTI, and INCORONATA GALASSO ... However, archaeological (KAPLAN Æ KAPLAN 1988), biochemical (GUTIERREZ SALGADO al. 1995) and molecular data (JAcoB &amp;amp;amp; al.... more
... Lima beans, Phaseolus lunatus (Fabaceae) LUCIA LIOI, CONCETTA LOTTI, and INCORONATA GALASSO ... However, archaeological (KAPLAN Æ KAPLAN 1988), biochemical (GUTIERREZ SALGADO al. 1995) and molecular data (JAcoB &amp;amp;amp; al. 1995, NIEN~UIS &amp;amp;amp; al. ...
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We describe the characterisation and the chromosomal localisation of two repeated DNA sequences, named pLc30 (466 bp long, 64% AT residues) and pLc7 (408 bp long, 61% AT residues), isolated from lentil (Lens culinaris ssp. culinaris)... more
We describe the characterisation and the chromosomal localisation of two repeated DNA sequences, named pLc30 (466 bp long, 64% AT residues) and pLc7 (408 bp long, 61% AT residues), isolated from lentil (Lens culinaris ssp. culinaris) genomic DNA. The pLc30 family is characterised by four internal repeats organised in a head-to-tail orientation, whereas the pLc7 contains many short direct subrepeats. The two families do not share significant sequence similarity. The distribution of these repetitive sequences in different Lens species and in other legumes was investigated. pLc30 is present in all Lens species investigated but absent from other genera examined. In contrast, pLc7 is present also in the genome of other legumes. As determined by FISH, the pLc30 sequence hybridises on six out of seven lentil chromosome pairs, while pLc7 hybridises on one only. The distribution of the nine different hybridisation sites of pLc30 allows the discrimination of all seven chromosome pairs and the...
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Page 1. Characterisation of structural genes involved in phytic acid biosynthesis in common bean (Phaseolus vulgaris L.) Marzia Fileppi • Incoronata Galasso • Giovanni Tagliabue • Maria Gloria Daminati • Bruno Campion • Enrico Doria •... more
Page 1. Characterisation of structural genes involved in phytic acid biosynthesis in common bean (Phaseolus vulgaris L.) Marzia Fileppi • Incoronata Galasso • Giovanni Tagliabue • Maria Gloria Daminati • Bruno Campion • Enrico Doria • Francesca Sparvoli ...
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Abstract A BAC library from common bean has been used in order to isolate the entire multigene BowmanBirk serine protease inhibitor family and to study its genome organization. Using a previously isolated trypsin/chymotrypsin inhibitor... more
Abstract A BAC library from common bean has been used in order to isolate the entire multigene BowmanBirk serine protease inhibitor family and to study its genome organization. Using a previously isolated trypsin/chymotrypsin inhibitor nucleotide sequence as probe, two ...
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... Chromosomal similarities, as resulting from kary-omorphological analysis, probably reflect phylefic affinities. ... to be on a chromo-somal basis, but rather on a genetic interference one, since hybrid zygotes are formed, but embryos... more
... Chromosomal similarities, as resulting from kary-omorphological analysis, probably reflect phylefic affinities. ... to be on a chromo-somal basis, but rather on a genetic interference one, since hybrid zygotes are formed, but embryos fail to fully develop (Barone &amp;amp;amp;amp; Ng, 1990; Del ...
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ABSTRACT A research project funded by Regione Lombardia provided the opportunity to test an integrated biorefinery model based on flax and hemp crops. The exploitation of the oil, the fiber and all the residues made the creation of a wide... more
ABSTRACT A research project funded by Regione Lombardia provided the opportunity to test an integrated biorefinery model based on flax and hemp crops. The exploitation of the oil, the fiber and all the residues made the creation of a wide bio-products portfolio possible.
... The rDNA sequences have been used as chromosome-specific markers to map and to tag particular chromosomes (MALUSZYNSKA and HESLOP-HARRISON 1993 a, b and references therein; OSUJI et al. 1998; TAKETA et al. 1999). ...
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Research Interests: Genetic Engineering, Genetic Diversity, Latin America, Developing Country, Maize, and 16 moreDisease resistance, Marker assisted selection, Genetic Map, Zinc, Iron, Agronomic Traits, Fluorescent in situ hybridization, Molecular Marker, Quantitative Trait Loci, Common bean, Indexation, Dietary Protein, Target Localization, Simple Sequence Repeat, Random amplified polymorphic DNA, and DNA sequence
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Page 1. Development of genomic simple sequence repeat markers for linseed using next-generation sequencing technology Sandip M. Kale Varsha C. Pardeshi Narendra Y. Kadoo Prakash B. Ghorpade Murari M. Jana Vidya S. Gupta ...
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ABSTRACT Camelina sativa (L.) Crantz in the last few years is garnering a lot of attention as a biofuel and as raw material for the chemical industry due to its high oil productivity. However the high percentage of polyunsaturated fatty... more
ABSTRACT Camelina sativa (L.) Crantz in the last few years is garnering a lot of attention as a biofuel and as raw material for the chemical industry due to its high oil productivity. However the high percentage of polyunsaturated fatty acids of camelina oil (over 50%), which is rich in linolenic acid (37–40%) limits its commercial value and large-scale production. To improve the oil quality and its oxidative stability the methyl esters have been selectively hydrogenated using a non-toxic and non-pyrophoric heterogeneous copper catalyst (Cu/SiO2 or Cu/Al2O3). Our results showed that both catalysts are able to reduce the linolenic acid content below 1% while selectively increasing the monounsaturated one.
ABSTRACT A genomic DNA library enriched with GA/TC repeats from Camelina sativa variety Calena has been analysed. After sequencing of about 200 randomly selected clones, approximately 60 % of them showed to contain simple or compound... more
ABSTRACT A genomic DNA library enriched with GA/TC repeats from Camelina sativa variety Calena has been analysed. After sequencing of about 200 randomly selected clones, approximately 60 % of them showed to contain simple or compound micro-satellites with a high number of repeats. Among all microsatellite markers analysed 15 primer pairs amplified polymorphic fragments. Forty C. sativa accessions of different origin were genotyped with 15 microsatellite markers that generated 134 alleles with an average of 8.93 alleles per locus. The observed heterozygosity (Ho) among the accessions ranged from 0.0 to 0.15 with an average of 0.0370, whereas the average of expected heterozygosity (He) among accessions was 0.2769. The analysis of the average total heterozygosity (H T = 0.651), the intrapopulation genetic diversity (H S = 0.260), the interpopulation genetic diversity (D ST = 0.391) and the coefficient of genetic differentiation among populations (G ST = 0.574) demonstrated that 57.4 % of the genetic diversity is among the accessions, while 42.6 % resides within them. Phylogenetic tree of the 40 C. sativa accessions was constructed based on Nei&amp;#39;s genetic distance. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram shows, except for CAM108 and CAM170, a clear discrimi-nation among C. sativa accessions grouping them in five subgroups. ANOVA analysis indicates significant differences in some biochemical and agronomic parameters among the C. sativa accessions grouped according to Nei&amp;#39;s genetic distance. The result of the Tukey HSD test demonstrated that the A4 subgroup showed a significant higher TWS and linoleic acid (LA) content, while the subgroup A1 showed a significant higher linolenic and lower LA content compared to the remaining groups.
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ABSTRACT Camelina sativa is an alternative oilseed crop that is shown to be adaptable to marginal areas and suitable for low-input cultivation. Moreover, the species shows a good productivity and interesting agronomic features. As a... more
ABSTRACT Camelina sativa is an alternative oilseed crop that is shown to be adaptable to marginal areas and suitable for low-input cultivation. Moreover, the species shows a good productivity and interesting agronomic features. As a result of particular characteristics, the oil produced by this species has been shown to be suitable for many food and non-food applications. Despite the interesting characteristics of this plant, a detailed description of the phenological growth stages of C. sativa has never been performed. A precise and univocal description of the different growth stages of this crop will provide a useful tool for several agronomic and research activities. In this article, the description of the phenological development of C. sativa is proposed utilising the extended BBCH (Biologische Bundesantalt, Bundessortenamt and Chemische Industrie) scale (Meier et al., 2009). The different phenological growth stages are described, utilising both the two- and three-digit BBCH coding system. This article is supplemented by drawings of the most representative stages.