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Nidhal Marzougui

The present study aimed to: (i) identify helminth eggs and protozoan cysts in wastewater samples to which both human and animals could be exposed when they are reused in agriculture; and (ii) evaluate the efficiency of their removal by... more
The present study aimed to: (i) identify helminth eggs and protozoan cysts in wastewater samples to which both human and animals could be exposed when they are reused in agriculture; and (ii) evaluate the efficiency of their removal by two wastewater treatment systems (activated sludge and lagoon). For these purposes, 234 wastewater samples (117 raw, 117 treated) were collected from 20 wastewater treatment plants (WWTPs) in Tunisia over a one-year sampling period. Parasitic load was enumerated by the modified Bailenger method. The results showed that helminth eggs found were Nematodes mainly represented by Ascaris sp. (95%), Hookworm species (50%), and Enterobius vermicularis (45%). For Cestodes, species identified were Taeniid eggs (85%), Hymenolepis nana (70%), Hymenolepis diminuta (55%) and digestive Strongyles. Among protozoa, Giardia sp., Entamoeba histolytica/dispar/moshkovskii and Entamoeba coli cysts were found in 100% of raw wastewater samples. The overall removal efficienc...
Opuntia ficus-indica (OFI) is a high added value species which different organs are used in several fields, including water purification. The present study intended to treat contaminated well water using naturally prepared coagulant from... more
Opuntia ficus-indica (OFI) is a high added value species which different organs are used in several fields, including water purification. The present study intended to treat contaminated well water using naturally prepared coagulant from the cladodes of four local OFI populations in Tunisia. Physicochemical characterization of the water was carried out before and after treatment. The treatment applied allowed to decontaminate the well water studied. Most of the parameters tested were significantly influenced by the "population used" factor. The treatment by the "Amless" population of OFI resulted in the best reduction rates of the contents of Mg, Na, Cu, and Pb. "Loki" population allowed obtaining the best pH and EC reductions. The “Violet” population resulted in the highest COD, BOD5, and TSS reductions. The effectiveness and simplicity of this treatment method would encourage farmers to use it to improve the quality of the well water that is used for ...
Assessment of the contributions of womens agricultural development groups: a survey of women in the Zaghouan governorate in Tunisia. Agricultural development groups were created in Tunisia to involve members in local development, protect... more
Assessment of the contributions of womens agricultural development groups: a survey of women in the Zaghouan governorate in Tunisia. Agricultural development groups were created in Tunisia to involve members in local development, protect and rationalize natural resources. They take over from the administrations activities and ensure cooperation and exchange with national and international agricultural organizations. The objective of this work is to analyze the contribution of the Womens Agricultural Development Groups (WADGs) in improving the living conditions of rural women in Oued Sbaihia and Nadhour regions in the governorate of Zaghouan. Specific surveys of 40 members and 25 not members of the two WADGs were carried out. Results showed that the residence place of the women surveyed had no significant impact on whether or not to join the WADG. The statistical study revealed a significant association between the activity nature practiced by the subjects surveyed and their percepti...
ABSTRACT The study aims the creation of the pure barley variety, tolerant to the abiotic stress by anther culture. This study was concerned two variety of barley: cultivar Ardhaoui, selected for their best tolerance to the abiotic stress... more
ABSTRACT The study aims the creation of the pure barley variety, tolerant to the abiotic stress by anther culture. This study was concerned two variety of barley: cultivar Ardhaoui, selected for their best tolerance to the abiotic stress (salt and drought) collected from Tunisia, and cultivar Arige, from Syria, sensitive for the two types of stress. The androgenic aptitude was evaluated in the parents (Ardhaoui and Arige) and their hybrids (F1) by anther culture. The stage of setting in anther culture was given on the basis of morphological and cytological criteria. The result shows that the capacity of regeneration of cal differs from a genotype with another (it varies from 50% at the hybrids with 40% in the parent Arige). Following the results, it is important to announce that the rate of albinism which we observed is height, it especially exceeds 70% from Ardhaoui; it can be explained by the combination of several factors. Finally we can deduced that the majority of the regenerated planted are diploid (70%).
ABSTRACT Polyploid plants are distinguished from diploids by an improved photosynthetic capacity related to the increase of the photosynthetic enzymes and pigments quantities. The aim of this work was to study the physiological... more
ABSTRACT Polyploid plants are distinguished from diploids by an improved photosynthetic capacity related to the increase of the photosynthetic enzymes and pigments quantities. The aim of this work was to study the physiological performances accompanying the experimental polyploidisation of Trigonella foenumgraecum by comparing the induced autotetraploid, the diploid resulting from the treated plants and the parent. Total chlorophylls content, photosynthesis rate, transpiration rate and stomatal conductance were measured for each genotype. Results showed highly significant variations (p < 0.01) between the different genotypes for the studied physiological characters. PCA and UPGMA method structured the genotypes in two groups. The first group is formed by the autotetraploids and the second is consisted by the parents and the diploids resulting from the treated plants.
There is a great need to find cheaper but still efficient treatment methods for wastewater. This study aimed to test the purifying performance of three different Moringa oleifera varieties that were cultivated in Tunisia on raw (RUW) and... more
There is a great need to find cheaper but still efficient treatment methods for wastewater. This study aimed to test the purifying performance of three different Moringa oleifera varieties that were cultivated in Tunisia on raw (RUW) and secondary treated urban wastewater (TUW). The seeds of the Mornag, Egyptian, and Indian varieties were powdered, added to the water (at concentrations of 0, 50, 100, and 150 mg·L−1), and stirred for 45 min at 120 rpm, and then left to settle for two hours. A physicochemical characterization of the wastewater was carried out before and after treatment. The investigated treatments decontaminated both types of urban wastewater. The best treatments were obtained with the Egyptian variety (at 150 mg·L−1), which excelled at the reduction of EC, TSS, BOD5, Cl, SO4, Ca, Na, Cd, and Fe in RUW and BOD5, EC, Na, Mg, Cl, and Cd in TUW. High amounts of TKN was found in both types of Moringa-treated wastewater, meaning that it could be used in agricultural irriga...
Polyploidy plays an important role in plant evolution and constitutes an important mechanism of diversification and creation of genetic variability. Artificial polyploidy can be induced using the colchicine. The aim of this study was to... more
Polyploidy plays an important role in plant evolution and constitutes an important mechanism of diversification and creation of genetic variability. Artificial polyploidy can be induced using the colchicine. The aim of this study was to determine the Trigonella foenum-greacum (2n = 16) population which resists better to colchicine treatment and which has the highest rate of polyploid formation among 38 populations collected from different Tunisian regions. The comparative study between treated and control plants showed significant increases (p < 0.05) for the parameters survival rate, rate of malformed leaves appearance, stem height and seeds number by pod. The 38 treated populations showed significant variations (p < 0.05) between them for the parameters survival rate, branches number, stem height and seeds number by pod. Results also showed that treatment of the shoot meristem by colchicine allowed to obtain diploid (2n = 16) and mixoploid plants having mixoploid branches (4...
RAPD (Random Amplified Polymorphic DNA) markers were applied to evaluate genetic diversity of South Tunisian barley accessions. Of the 49 fragments generated by 4 selected primers (OPA-04, OPA-18, UBC-490 and UBC-534) 42 appeared to be... more
RAPD (Random Amplified Polymorphic DNA) markers were applied to evaluate genetic diversity of South Tunisian barley accessions. Of the 49 fragments generated by 4 selected primers (OPA-04, OPA-18, UBC-490 and UBC-534) 42 appeared to be polymorphic. These RAPD bands were used to calculate Jaccard’s distance coefficient for cluster analysis using an arithmetic averaging algorithm (UPGMA). The genetic distance of the 20 studied accessions ranged between 0.25 and 0.68. Of the accessions 39.5% have a wide genetic distance (more than 0.6) and 60.5% are characterized by a distance ranged between 0.3-0.6. The analysis of the hierarchical tree shows the presence of four ramifications thus translating great genetic diversity.
In Tunisia, the mulberry tree is limited to certain orchards in the coastal zones and the oases. In spite of its various uses, this species is threatened by the extension of the urbanization. It is in order to contribute to a better... more
In Tunisia, the mulberry tree is limited to certain orchards in the coastal zones and the oases. In spite of its various uses, this species is threatened by the extension of the urbanization. It is in order to contribute to a better knowledge of the variability of mulberry tree in the Tunisian South than one makes a pomological characterization of the sheet and fruit. This study shows the existence of the differences on the level of the form, the size and the color of the sheets and the fruits. The leaf area varies from 44 cm 2 for the cultivar Elmay I to 254.68 cm 2 for that of Jara. The fruit weight varies 1.091 g for Midoune I with 6.245 g for Gafsa III. The studied cultivars belong to the species Morus alba, M. nigra or M. rubra.
Rumex vesicaruis L. has attracted the attention of many researchers because of its phyto constituents and medicinal properties. The importance of Rumex was based on its biological activities such as antimicrobial and anti-inflammatory. In... more
Rumex vesicaruis L. has attracted the attention of many researchers because of its phyto constituents and medicinal properties. The importance of Rumex was based on its biological activities such as antimicrobial and anti-inflammatory. In the present study, antioxidant potential of Rumex vesicaruis extract was evaluated by using DPPH radical scavenging assays. The methanol extract showed significant free radical scavenging activities: stems and flowers of R. vesicaruis possess higher scavenging capacity (53.29 % and 50.24%) compared to the leaves (22.21%). Several phenolic compounds were detected by HPLC coupled with diode-array detection. 4-O-caffeoyl quinic acid (21.984mg/100g) and caffeic acid (12.200mg/100g) were identified as the main phenolic compounds present in the stem extract. Chlorogenic acid (718.795mg/100g) and quercetin-3-o-galactoside (165.457mg/100g) were predominant in flowers extract and the dominant phenolic compound in leaves extract was the quinic acid (48.116 m...
Trigonella foenum graecum is an annual flowering species largely cultivated in the Mediterranean basin countries, central Asia, central Europe, United Kingdom and United States. The grain is rarely grown outside their native habitat. This... more
Trigonella foenum graecum is an annual flowering species largely cultivated in the Mediterranean basin countries, central Asia, central Europe, United Kingdom and United States. The grain is rarely grown outside their native habitat. This work aims to study the diversity of 38 Tunisian cultivars of this plant using Inter Simple Sequence Repeat markers. Cultivars were collected from upper-semi arid, sub humid and lower arid Tunisian bioclimatic stages. A set of 12 ISSR primers was used for cultivars initial screening. Four primers gave amplification, detected intraspecific variation and revealed 144 bands. Sixty-two bands were polymorphic with an average of 15.5 bands per primer and 60.5% of polymorphism. The percentage of polymorphic bands varied from 30.7% for (CA) 6 GT primer to 73.3% for (GAG) 3 GC primer. The highest heterozygosity value (0.95) was obtained with (GA) 6 CC primer and the lowest one (0.5) was observed with (CA) 6 GT primer. The highest marker index value (3.6) was...
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In agricultural systems, biodiversity includes diversity within species and among species and provides many benefits for production, resilience and conservation. This article aims to study the diversity of Trigonella foenum-graecum L., an... more
In agricultural systems, biodiversity includes diversity within species and among species and provides many benefits for production, resilience and conservation. This article aims to study the diversity of Trigonella foenum-graecum L., an annual herb of Leguminosae with a worldwide distributed culture, among thirty eight local populations collected from different Tunisian regions. The variability analysis was based on morphological characters, compositions of minerals in leaves and vitamins in seeds, and molecular profile. Morphological parameters consisted of vegetative and reproductive characters. Mineral analysis concerned sodium, potassium, iron, calcium and magnesium leaves contents. The analyzed vitamins contents in seeds were B1, B9 and C vitamins and molecular study was carried out by ISSR technique. The structure of the studied populations was established by the principal compound analysis (PCA) and by the unweighted pair group method using arithmetic means (UPGMA). Both an...
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Irrational use of chemical method for crop protection, presents increasingly serious risks for human health and the environment. Droplet size and meteorological parameters are key factors to both environmental contamination and pest... more
Irrational use of chemical method for crop protection, presents increasingly serious risks for human health and the environment. Droplet size and meteorological parameters are key factors to both environmental contamination and pest control efficacy. The objective of this study is to assess the impact of the nozzle use parameters, the operating pressure and the wind speed on droplet foliage deposition (retention) and soil deposition (losses), when treating artichoke. Several combinations were tested in a wind tunnel and in the field, under Mediterranean microclimatic conditions, using a fluorescent dye as a substitute for pesticide. Multiple regression models were built from tunnel data to predict foliage deposition and soil deposits, with determination coefficients of 0.96. Thus, models are able to simulate pesticide deposition on artichoke leaves and soil deposition, depending on sprayer parameters and wind speed. Foliage deposition and soil deposits rates ranged from 30 to 52% an...
RAPD (Random Amplified Polymorphic DNA) markers were applied to evaluate genetic diversity of South Tunisian barley accessions. Of the 49 fragments generated by 4 selected primers (OPA-04, OPA-18, UBC-490 and UBC-534) 42 appeared to be... more
RAPD (Random Amplified Polymorphic DNA) markers were applied to evaluate genetic diversity of South Tunisian barley accessions. Of the 49 fragments generated by 4 selected primers (OPA-04, OPA-18, UBC-490 and UBC-534) 42 appeared to be polymorphic. These RAPD bands were used to calculate Jaccard's distance coefficient for cluster analysis using an arithmetic averaging algorithm (UPGMA). The genetic distance of the 20 studied accessions ranged between 0.25 and 0.68. Of the accessions 39.5% have a wide genetic distance (more than 0.6) and 60.5% are characterized by a distance ranged between 0.3-0.6. The analysis of the hierarchical tree shows the presence of four ramifications thus translating great genetic diversity.
Trigonella foenum graecum L. (Leguminosae) is known for its forage potential and has several pharmacological effects. The objective of this study was to evaluate the genetic diversity of 38 Tunisian cultivars of T. foenum graecum based on... more
Trigonella foenum graecum L. (Leguminosae) is known for its forage potential and has several pharmacological effects. The objective of this study was to evaluate the genetic diversity of 38 Tunisian cultivars of T. foenum graecum based on vegetative and reproductive ...
... Auteur(s) / Author(s). GUASMI Ferdaous (1) ; TOUIL Leila (1) ; FERES Khadija (1) ; MARZOUGUI Nidhal (1) ; ELFALLEH Walid (1) ; FERCHICHI Ali (1) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). ... Langue / Language.... more
... Auteur(s) / Author(s). GUASMI Ferdaous (1) ; TOUIL Leila (1) ; FERES Khadija (1) ; MARZOUGUI Nidhal (1) ; ELFALLEH Walid (1) ; FERCHICHI Ali (1) ; Affiliation(s) du ou des auteurs / Author(s) Affiliation(s). ... Langue / Language. Anglais Editeur / Publisher. ...
Abstract Trigonella foenum graecum is used as spice and forage, and it is known for its therapeutic effects in traditional medicine. The seeds are rich in vitamins A, B and C. This work aims to study the diversity of 38 Tunisian... more
Abstract Trigonella foenum graecum is used as spice and forage, and it is known for its therapeutic effects in traditional medicine. The seeds are rich in vitamins A, B and C. This work aims to study the diversity of 38 Tunisian Trigonella foenum graecum cultivars using ...
ABSTRACT
ABSTRACT Trigonella foenum graecum L. is a diploid leguminous plant (2n = 16) used as forage and vegetable. The objective of this study was to produce a polyploid cultivar of this plant and to evaluate its morphological and chemical... more
ABSTRACT Trigonella foenum graecum L. is a diploid leguminous plant (2n = 16) used as forage and vegetable. The objective of this study was to produce a polyploid cultivar of this plant and to evaluate its morphological and chemical potentialities. Polyploidy induction was carried out by using a 0.5% colchicine solution. Tetraploids and diploids seeds resulting from the obtained mixoploids and the control seeds were used for the comparative study extended on 4 plant generations. The autotetraploid cultivar has larger leaf area and larger productivity compared to the diploids concerning seed number, pod number and branch number. Its leaves are richer in potassium, sodium, calcium and phosphorus. Polyploidy induction is an effective method to increase the yield and the mineral contents of Trigonella foenum-graecum.
ABSTRACT This study describes the effect of some parameters on anther culture of barley to optimize the plant regeneration condition of this species. The embryo formation and plant regeneration from anthers of three barley cultivars... more
ABSTRACT This study describes the effect of some parameters on anther culture of barley to optimize the plant regeneration condition of this species. The embryo formation and plant regeneration from anthers of three barley cultivars (‘Igri’, ‘Saida’ and ‘Libya’) were investigated. The effects of length of flag leaves, stage of microspore development, and pre-treatments (mannitol or cold pre-treatment) of anthers were investigated. Results showed significant responses. Anthers at the mid uninucleate to mid-late uninucleate stage gave the best anther culture response showing 80, 60, and 30% anther development in ‘Igri’, ‘Saida’ and ‘Libya’ barley cultivars, respectively. The use of mannitol (0.3 M) or 20 days cold pre-treatments showed the best results for embryo and green-plant production.
ABSTRACT A total of 20 accessions of barley (Hordeum vulgare L.) from arid regions of Tunisia were collected and evaluated ex situ in experimental fields (Medenine, South Tunisia). Morphological traits and molecular marker (Random... more
ABSTRACT A total of 20 accessions of barley (Hordeum vulgare L.) from arid regions of Tunisia were collected and evaluated ex situ in experimental fields (Medenine, South Tunisia). Morphological traits and molecular marker (Random Amplified polymorphic DNA) were applied to evaluate genetic diversity of these accessions. The study showed the existence of a highly significant phenotypical variability. Some parameters (production of straw and grain yield) appeared as good criteria to differentiate particular accessions. Concerning molecular variability, 49 RAPD bands were used to calculate Jaccard's distance coefficient for cluster analysis using an arithmetic averaging algorithm (UPGMA). The genetic distance of the 20 studied accessions ranged between 0.25–0.68. The analysis of the hierarchical tree shows the presence of four ramifications thus translating great genetic diversity.
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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY