Eloisa Caldas
Universidade de Brasília - UnB, Ciencias Farmaceuticas, Faculty Member
Research Interests: Business, Communication, Risk, Comunicação, Legislation, and 4 moreAgrochemicals, Legislação, Risco, and Agroquímicos
Mercury is a toxic metal, ubiquitous in nature; it is excreted in breast milk from exposed mothers and may affect infant neuro-development. In this study, 224 breast milk samples provided by eight human milk banks in the Federal District... more
Mercury is a toxic metal, ubiquitous in nature; it is excreted in breast milk from exposed mothers and may affect infant neuro-development. In this study, 224 breast milk samples provided by eight human milk banks in the Federal District of Brazil were analyzed for total mercury (THg), of which 183 were also analyzed for methyl mercury (MeHg), the most relevant form of this metal for the breastfed infants. Samples were acid digested in a microwave oven and THg determined by atomic fluorescence spectrometry (LOQ of 0.76μg/L). Samples were lyophilized, ethylated and MeHg determined in a MERX automated system (LOQ of 0.10μg/L). Inorganic mercury (IHg) levels were estimated from the THg and MeHg determined in the samples. Most of the samples were collected 1-2 months postpartum, with 38% during the first month. Over 80% of the samples had THg values above the LOQ, reaching a maximum of 8.40μg/L, with a mean of 2.56μg/L. On average, MeHg accounted for 11.8% of THg, with a maximum of 97.4...
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RESUMO Os ditiocarbamatos são os fungicidas mais utilizados e mais detectados em alimentos no Brasil. Este estudo teve como objetivos analisar os níveis de resíduos de ditiocarbamatos em hortaliças produzidas na região de Vargem Bonita,... more
RESUMO Os ditiocarbamatos são os fungicidas mais utilizados e mais detectados em alimentos no Brasil. Este estudo teve como objetivos analisar os níveis de resíduos de ditiocarbamatos em hortaliças produzidas na região de Vargem Bonita, Distrito Federal, e comparar os níveis encontrados com os limites máximos de resíduos (LMR) estabelecidos para esses alimentos. Foram coletadas 84 amostras de diferentes tipos de hortaliças diretamente de 23 produtores no período de junho a novembro de 2018. As análises foram realizadas utilizando o método espectrofotométrico, que determina o nível de CS2 produzido pela degradação em meio ácido do ditiocarbamato presente na amostra. No total, 21 amostras (25%) continham resíduos de ditiocarbamatos acima do Limite de Quantificação do método (LOQ, 0,05 mg/kg CS2). Cinco das 27 amostras de alface analisadas (18,5%) continham resíduos, com níveis de 0,06 a 0,50 mg/kg de CS2, abaixo do LMR para essa cultura. Resíduos também foram encontrados em amostras d...
This cross-sectional study aimed to assess the risk perception of three different population groups in the Federal District, Midwest Brazil, regarding chemical and technological risks related to food. An objective questionnaire was... more
This cross-sectional study aimed to assess the risk perception of three different population groups in the Federal District, Midwest Brazil, regarding chemical and technological risks related to food. An objective questionnaire was applied from May 2018 to January 2020 to 1,000 individuals in supermarkets, universities (only students) and hospitals/clinics. Risk perception was assessed through five general questions, and the degree of worry regarding 11 food-related hazards measured by a three-point scale (low, medium and high). The impact of belonging to a group and of sociodemographic variables on the worry level was assessed by multinomial logistic regression and expressed as Odds Ratio (OR). Over 80% of the participants had high or medium worry level about the presence of chemicals in food, hospital/clinic group having significantly higher level than the university group. Heavy metals had a significant higher worry score than all other hazards (2.76 ± 0.55) and was the only hazard that was not impacted by the group or any sociodemographic variable. Nanotechnology had a significantly lower score than all others hazards and, along with mycotoxins, was the most unfamiliar term to the respondents. In the adjusted multinomial model, older individuals, those interviewed in hospital/clinic, and women showed significantly greater risk perception to most hazards. Income and education exerted less effect, except for the technologies, which significantly caused more worry among individuals with lower income and/or education. The results of this study can help government authorities in the implementation of effective risk communication strategies aimed at different population segments.
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Abstract Herbicide use, mainly glyphosate, has been intense in worldwide agriculture, including in the Brazilian Amazon region. This study aimed to validate a method for determining glyphosate and its degradation product, AMPA, and... more
Abstract Herbicide use, mainly glyphosate, has been intense in worldwide agriculture, including in the Brazilian Amazon region. This study aimed to validate a method for determining glyphosate and its degradation product, AMPA, and glufosinate by HPLC-FL in 58 water samples collected at the Santarém plateau region (Planalto Santareno), in the western of Pará state, Brazil. The method involves filtration and direct injection in the HPLC-FL for AMPA analysis, or previous concentration (10×) by lyophilization for glufosinate and glyphosate analysis. Analytes were oxidized and complexed with o-phthalaldehyde and 2-mercaptoethanol in a post-column reaction before fluorescence detection. LOQs for AMPA, glyphosate and glufosinate were established at 0.5, 0.2 and 0.3 μg L−1, respectively. A total of 58 samples were collected. Glyphosate and glufosinate were not detected in any of the 30 surface water samples collected in 2015 (<LOQ), and AMPA was detected in 6 samples (0.65–1.93 μg L−1). A total of 28 ground and surface water samples were collected in 2017 and analyzed for glyphosate, which was detected in 11 samples (7 ground water samples), with concentrations between 1.5 and 9.7 μg L−1. A continuous pesticide monitoring of the Amazonian water system is essential to guarantee the preservation of this important ecosystem.
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Ayahuasca is a psychoactive concoction prepared from the plants Banisteriopsis caapi and Psychotria viridis which are used ancestrally by Amazonian Indian populations and more recently, by Christian religious groups in Brazil and other... more
Ayahuasca is a psychoactive concoction prepared from the plants Banisteriopsis caapi and Psychotria viridis which are used ancestrally by Amazonian Indian populations and more recently, by Christian religious groups in Brazil and other countries. The aims of the present study were to identify the effects of ayahuasca on zebrafish embryo development and neurobehavior. Toxicity and developmental endpoints for zebrafish embryos were assessed from 0 to 1000 mg/L over 96 h of exposure. The effects on locomotor activity of zebrafish larvae were assessed using a video tracking system (ZebraBox) from 0 to 20 mg/L and after 120 and 144 h of exposure. The LC of ayahuasca in zebrafish was determined as 236.3 mg/L. Ayahuasca exposure caused significant developmental anomalies in zebrafish embryos, mainly at the highest concentration tested, including hatching delay, loss of equilibrium, edema and the accumulation of red blood cells. Embryo behavior was also significantly affected, with decrease...
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The worldwide occurrence of aflatoxins (AFB1, AFB2, AFG1, AFG2), genotoxic mycotoxins, in raw maize, rice, sorghum and wheat samples collected since the year 2000 was evaluated using published data and occurrence data from the GEMS/Food... more
The worldwide occurrence of aflatoxins (AFB1, AFB2, AFG1, AFG2), genotoxic mycotoxins, in raw maize, rice, sorghum and wheat samples collected since the year 2000 was evaluated using published data and occurrence data from the GEMS/Food database (https://extranet.who.int/gemsfood). Dietary risk assessments were conducted using GEMS/Food total aflatoxin occurrence and food consumption data obtained from the 17 Cluster Diets. Risk characterisation arising from aflatoxin exposure was conducted using both cancer risk and margin of exposure (MOE) approaches. A total of 89 publications were retrieved from the literature, reporting data related to 18,097 samples, of which 37.6% were positive for at least one aflatoxin. The total upper bound (UB) mean for all samples analysed was 13.6 μg/kg, and was higher for rice (24.6 μg/kg) and sorghum (25.9 μg/kg). Of data related to the analysis of 4,536 samples reported to GEMS/Food database, 12.7% were positive for at least one aflatoxin. The total ...
To investigate the access to medicines by Brazilian families by monetary and non-monetary acquisition data. This is a cross-sectional study based on data obtained from the 2008/2009 Brazilian Household Budget Survey. The units of... more
To investigate the access to medicines by Brazilian families by monetary and non-monetary acquisition data. This is a cross-sectional study based on data obtained from the 2008/2009 Brazilian Household Budget Survey. The units of assessment were households that participated in the survey and the data on the acquisition of medicines over the 30 days prior to the interviews. The medicines were classified according to the Anatomical Therapeutic Chemical classification system. Acquisition of medicines was reported by 82.9% of Brazilian households, with 2.38 medicines/household, and 0.72 medicine/individual. In the South and Southeast regions, the average acquisition was slightly greater than the national average (2.53 and 2.49, respectively). In 22.3% of Brazilian households, it was reported that a medicine was not acquired due to lack of financial resources, mainly in the North and Northeastern regions, and in rural areas. Approximately 15.0% of medicines were obtained with no costs, 9...
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... Available at http://dtr2004.saude.gov.br/nutricao/documentos/guia_alimentar_conteudo.pdf. Naska A, Fouskakis D, Oikonomou E, Almeida MDV, Berg MA, Gedrich K, Moreiras O, ... Naska A, Vassilis GS, Vasdekis VG, Trichopoulou A, Friel S,... more
... Available at http://dtr2004.saude.gov.br/nutricao/documentos/guia_alimentar_conteudo.pdf. Naska A, Fouskakis D, Oikonomou E, Almeida MDV, Berg MA, Gedrich K, Moreiras O, ... Naska A, Vassilis GS, Vasdekis VG, Trichopoulou A, Friel S, Leonhauser IU, Moreiras O, ...
This study describes the substances involved in fatal events that were investigated by the Forensic Medicine Institute of the Federal District, Brazil, from 2006 to 2008. The presence of pesticides was investigated in liver or stomach... more
This study describes the substances involved in fatal events that were investigated by the Forensic Medicine Institute of the Federal District, Brazil, from 2006 to 2008. The presence of pesticides was investigated in liver or stomach content samples by thin-layer chromatography; amphetamines, benzodiazepines, barbiturates, tetra-hydro-cannabinol (THC), cocaine and opioids were analyzed in urine samples by immunoassay. A total of 8736 exams were performed, of which 21.7% tested positive for at least one compound investigated. Men were involved in over 90% of positive cases; most individuals were between the ages of 18 and 30. Alcohol was detected in 47.4% of the blood samples, with over 30% presenting BAC levels higher than 1g/L. Cocaine was present in 21.6%, and THC in 17.5% of the urine samples analyzed. Pesticides were found in 13.3% of the 188 cases investigated, mostly aldicarb, which is the main component of chumbinho, an illegal rodenticide available in the country. Among pharmaceutical drugs, benzodiazepines were the most detected, with 29 samples testing positive (4.6% of the samples analyzed). Most of the individuals who died from traumatism or hypovolemia tested positive for alcohol. Death from intoxication/poisoning corresponded to 1.8% of all positive cases, mainly from the ingestion of pesticides. This is the first Brazilian study reporting data on toxic chemicals from postmortem material. The results confirm other epidemiological data that indicate pesticide as the major cause of fatal poisonings in Brazil.
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In Brazil, in the last 20 years, dietary risk assessments have been conducted on pesticides, mycotoxins, food additives, heavy metals (mainly mercury), environmental contaminants (mainly DDT) and acrylamide, a compound formed during food... more
In Brazil, in the last 20 years, dietary risk assessments have been conducted on pesticides, mycotoxins, food additives, heavy metals (mainly mercury), environmental contaminants (mainly DDT) and acrylamide, a compound formed during food processing. The objectives of this paper were to review these studies, discuss their limitations and uncertainties and identify the most critical chemicals that may pose a health risk to Brazilian consumers. The studies have shown that the cumulative intake of organophosphorus and carbamate pesticides by high consumers of fruits and vegetables may represent a health concern (up to 169% of the ARfD), although the benefits of consuming large portions of those foods most probably overcome the risks. High consumers of maize products may also be at risk due to the presence of fumonisin (355% of the PMTDI), a mycotoxin present at high levels in Brazilian maize. The studies conducted in the Brazilian Amazon have shown that riparian fish consumers are exposed to unsafe levels of mercury. However, this is a more complex issue, as mercury levels in the region are naturally high and the health benefits of a fish-based diet are well known. Studies conducted both in Brazil and internationally on acrylamide have shown that the exposure to this genotoxic compound, mainly from the consumption of French fries and potato chips, is of health concern. Reducing the population dietary exposure to toxic chemicals is a challenge for government authorities and food producers in all countries. Management strategies aimed at decreasing exposure to the critical chemicals identified in this review involve limiting the use or eliminating highly toxic pesticides, implementing good agricultural practices to decrease maize contamination by fumonisins, educating local fish-eating communities toward a fish diet less contaminated by mercury, and changing dietary habits concerning the consumption of fried potatoes, the main processed food containing acrylamide.
Research Interests: Diet, Brazil, Environmental Monitoring, Food Processing, Environmental Sciences, and 15 moreHumans, Brazilian Amazon, Heavy Metal, Fruit and vegetables, CHEMICAL SCIENCES, Exposure science, Chip, Food Additives, Health risk, Food Contamination, Food Additive, Environmental Exposure, Cumulant, Acrylamide, and Dietary Habits
... Id: 196811. Autor: Silva, Saulo Cardoso; Oliveira, Joäo Nascimento de; Caldas, Eloisa Dutra. Título: Aflatoxinas em alimentos comercializados no Distrito Federal de 1985 a 1995 / Aflatoxins in comercialized food at Federal District... more
... Id: 196811. Autor: Silva, Saulo Cardoso; Oliveira, Joäo Nascimento de; Caldas, Eloisa Dutra. Título: Aflatoxinas em alimentos comercializados no Distrito Federal de 1985 a 1995 / Aflatoxins in comercialized food at Federal District area from 1985 to 1995. Fonte: Rev. Inst. ...
OBJETIVO: Estudar o padrão de suscetibilidade do Aedes aegypti ao inseticida organofosforado temefós. MÉTODOS: Amostras de larvas de Ae. aegypti foram obtidas com armadilhas para oviposição, em oito cidades do Distrito Federal, nos anos... more
OBJETIVO: Estudar o padrão de suscetibilidade do Aedes aegypti ao inseticida organofosforado temefós. MÉTODOS: Amostras de larvas de Ae. aegypti foram obtidas com armadilhas para oviposição, em oito cidades do Distrito Federal, nos anos 2000 e 2001. As larvas foram submetidas à dose diagnóstica de 0,012 mg/l de temefós, segundo metodologia padronizada pela Organização Mundial da Saúde. As populações de campo foram testadas em paralelo com a cepa de referência Rockefeller e a cepa DIVAL, do insetário da Diretoria de Vigilância Ambiental. A concentração e a pureza das soluções de temefós foram analisadas por cromatografia gasosa. Os cálculos de correlação foram determinados pelo programa StatView - SAS Institute Inc., versão 5. Utilizou-se o teste t de Student para verificar diferenças de suscetibilidade, com níveis de significância, alfa=0,05. RESULTADOS: Em 2000, as populações de larvas de Ae. aegypti nas cidades de Taguatinga, Guará e Núcleo Bandeirante apresentaram-se resistentes ao temefós, com mortalidade de larvas entre 54,1 e 63,4%. As populações do Gama, Planaltina e Sobradinho apresentaram alterações nos níveis de suscetibilidade (mortalidade de 83,6 a 92,8%). A população de Ceilândia foi a única suscetível, com 98% de mortalidade. Em 2001, todas as populações testadas mostraram-se resistentes (44,4 a 66,4% de mortalidade). Nenhuma correlação significativa foi encontrada entre a suscetibilidade das populações e a distância entre essas cidades ou a quantidade de inseticida aplicado nos anos anteriores ao estudo. CONCLUSÕES: Os níveis de suscetibilidade do Ae. aegypti ao temefós vêm se alterando no Distrito Federal. É essencial a continuidade de programas de monitoramento da resistência desse vetor aos inseticidas para se garantir a eficiência dos programas de controle e a proteção da saúde humana.
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A probabilistic estimation of the exposure of the Brazilian population to the dithiocarbamate pesticides was performed using the Monte Carlo Risk Assessment program (MCRA 3.5). Residue data, as CS2, for 3821 samples were obtained from the... more
A probabilistic estimation of the exposure of the Brazilian population to the dithiocarbamate pesticides was performed using the Monte Carlo Risk Assessment program (MCRA 3.5). Residue data, as CS2, for 3821 samples were obtained from the Brazilian national monitoring program on pesticide residues and from the monitoring program conducted in the Distrito Federal on rice, beans and nine fruits and vegetables. Food consumption data were obtained from a Brazilian household budget survey conducted between 2002 and 2003. Processing factors for washing, peeling or cooking were applied to the residues found in the crops. Daily intakes at the highest percentiles for the general population reached a maximum of 2.0 microg CS2/kg body weight per day (upper band of the 95% confidence interval at P99.99). Tomato, rice, apple and lettuce were the commodities which contributed most to the intake. Based on the registered uses and the toxicological profile of dithiocarbamates, the risk from exposure was evaluated assuming that all residues came from the use of ethylene-bis-dithiocarbamate (EBDC) or that a fraction of it came from the use of propineb. For this last scenario, a cumulative risk assessment was conducted. In the first scenario, the highest intake reached up to 11.9% EBDC ADI for the general population and up to 31.1% ADI for children. When 30% of the residues were considered as coming from propineb use, the values were 15.2% and 39.7% ADI, respectively.
Research Interests: Diet, Brazil, Environmental Monitoring, Food Analysis, Exposure Assessment, and 15 moreHumans, Food Consumption, Data Collection, Household Budget Survey, Eating, Fruit and vegetables, Body Weight, Food Contamination, Food Sciences, Food and Chemical Toxicology, Distrito Federal, Environmental Exposure, General Population, Confidence Interval, and Cumulant
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Recebido em 12/09/2013; aceito em 28/02/2014; publicado na web em 17/06/2014 ENVIRONMENTAL RISK ASSESSMENT OF AQUATIC SYSTEMS AFFECTED BY PESTICIDE USE. Ecological risk assessment is a process evaluating the likelihood that adverse... more
Recebido em 12/09/2013; aceito em 28/02/2014; publicado na web em 17/06/2014 ENVIRONMENTAL RISK ASSESSMENT OF AQUATIC SYSTEMS AFFECTED BY PESTICIDE USE. Ecological risk assessment is a process evaluating the likelihood that adverse ecological effects may occur or are occurring as a result of exposure to one or more substances. Relevant information needed to predict risks from pesticides includes their physical chemical properties, mobility in soil, persistence in the environment, and bioaccumulation and toxicity in relevant organisms. Although currently a requirement for pesticide registration in Brazil, this process is not yet fully implemented in the country. This review aims to outline the principles of environmental risk assessment for pesticides, focusing on aquatic organisms, and to discuss the studies and efforts conducted in Brazil in this area. INTRODUÇÃO Os agrotóxicos estão entre os principais instrumentos do atual modelo de desenvolvimento da agricultura brasileira, cent...
Ayahuasca, a psychoactive beverage used by indigenous and religious groups, is generally prepared by the coction of Psychotria viridis and Banisteriopsis caapi plants containing N,N-dimethyltryptamine (DMT) and β-carboline alkaloids,... more
Ayahuasca, a psychoactive beverage used by indigenous and religious groups, is generally prepared by the coction of Psychotria viridis and Banisteriopsis caapi plants containing N,N-dimethyltryptamine (DMT) and β-carboline alkaloids, respectively. To investigate the acute toxicity of ayahuasca, the infusion was administered by gavage to female Wistar rats at doses of 30X and 50X the dose taken during a religious ritual, and the animals observed for 14 days. Behavioural functions were investigated one hour after dosing at 15X and 30X using the open field, elevated plus maze, and forced swimming tests. Neuronal activation (c-fos marked neurons) and toxicity (Fluoro-Jade B and Nissl/Cresyl staining) were investigated in the dorsal raphe nuclei (DRN), amygdaloid nucleus, and hippocampal formation brain areas of rats treated with a 30X ayahuasca dose. The actual lethal oral dose in female Wistar rats could not be determined in this study, but was shown to be higher than the 50X (which corresponds to 15.1mg/kg bw DMT). The ayahuasca and fluoxetine treated groups showed a significant decrease in locomotion in the open field and elevated plus-maze tests compared to controls. In the forced swimming test, ayahuasca treated animals swam more than controls, a behaviour that was not significant in the fluoxetine group. Treated animals showed higher neuronal activation in all brain areas involved in serotoninergic neurotransmission. Although this led to some brain injury, no permanent damage was detected. These results suggest that ayahuasca has antidepressant properties in Wistar female at high doses, an effect that should be further investigated.
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To evaluate risk perception, practices and attitudes on pesticide use in a rural population. Qualitative study conducted in Midwestern Brazil in February 2005. Seven focal groups (N=40), comprising five to seven individuals each,... more
To evaluate risk perception, practices and attitudes on pesticide use in a rural population. Qualitative study conducted in Midwestern Brazil in February 2005. Seven focal groups (N=40), comprising five to seven individuals each, discussed pesticide-related issues and included the presentation of a product container to support the discussion on labels. The discussions were tape recorded, transcribed and analyzed following the method for discourse analysis. The farmers studied were aware of the risks resulting from direct and indirect exposure to pesticides and many were concerned about potential environmental contamination. Interviewee's information on pesticides was limited to product dosage and was mostly conveyed by product retailers. They complained about the small print and technical language used in product labels but many were able to understand the pictograms and color toxicity codes. The farmers studied not always converted their risk perception and personal experiences...
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To conduct a chronic dietary risk assessment of the pesticides registered in Brazil up until 1999. The Theoretical Maximum Daily Intake (TMDI) for each pesticide was calculated using the Brazilian maximum residue limits and food... more
To conduct a chronic dietary risk assessment of the pesticides registered in Brazil up until 1999. The Theoretical Maximum Daily Intake (TMDI) for each pesticide was calculated using the Brazilian maximum residue limits and food consumption data from IBGE, the Brazilian Statistical Institute. The risk characterization was done comparing the TMDI with the acceptable daily intakes (ADI) from other countries and from the Codex Alimentarius. The TMDI was higher than the ADI (%ADI>100) at least in one Brazilian metropolitan region for 23 pesticides. Sixteen compounds are organophosphate insecticides, with methyl parathion having the TMDI exceeding the most toxicological parameter (%ADI N=9,300). Rice, beans, citrus and tomato were the commodities which most contributed to the ingestion. From the compounds under higher risk, only 6 were registered according to the Law 98.816/90, which concerns the use of pesticides in the country. The compounds identified in the study as presenting a p...
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The mycotoxin citreoviridin has been associated with the... more
The mycotoxin citreoviridin has been associated with the &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;yellow rice&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; disease, which caused cardiac beriberi in Japan. In Brazil, the consumption of contaminated rice was suspected to be involved in a recent beriberi outbreak. In this work, citreoviridin was produced by Penicillium citreonigrum, cultivated in 500 ml yeast extract sucrose (YES) liquid medium for 8 days at 25ºC, and the toxin extracted with chloroform from the liquid medium and the mycelium. A total of 15.3 g of crude extract was obtained from 48 culture flasks, with an estimated citreoviridin contend of 5.54 g, 74.3% being present in the mycelia. Semi-preparative HPLC of the crude extract yielded 27.1% citreoviridin. The HPLC-purified citreoviridin fraction was fully characterised by UV/VIS, FT-IR, (1)H- and (13)C-NMR, LC-MS/MS and LC-MSD TOF, and purity confirmed by gravimetric analysis. Isocitreoviridin was also produced by P. citreonigrum, accounting for about 10% of the citreoviridin present in the crude extract, most transformed into citreoviridin after 10 months under freezing conditions protected from light. Citreoviridin was shown to be stable under the same conditions, although it can suffer isomerisation after a longer storage period. Isomerisation is a potential source of variability in toxicological studies and purity of the material should be checked before study initiation.
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As notificações de intoxicações e tentativas de suicídio provocadas por agrotóxicos na microrregião de Dourados, Mato Grosso do Sul, entre 1992 e 2002, foram avaliadas baseando-se nos registros do Centro Integrado de Vigilância... more
As notificações de intoxicações e tentativas de suicídio provocadas por agrotóxicos na microrregião de Dourados, Mato Grosso do Sul, entre 1992 e 2002, foram avaliadas baseando-se nos registros do Centro Integrado de Vigilância Toxicológica da Secretaria de Saúde do estado. Foram notificadas 475 ocorrências no período, sendo 261 intoxicações (acidental ou ocupacional), 203 tentativas de suicídio e 11 eventos de causa indeterminada. O Município de Dourados apresentou a maior prevalência de intoxicações, por 100 mil habitantes, considerando a população rural, e Fátima do Sul a segunda maior prevalência de suicídios na microrregião. Correlações significativas foram encontradas entre intoxicação e tentativa de suicídio (r = 0,60; p < 0,05), e entre intoxicação e razão entre a área ocupada por culturas temporárias e área total do município (r = 0,68; p < 0,05). As intoxicações ocorreram predominantemente com homens (87,0%), mas a diferença entre tentativas de suicídio em homens e m...
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Research Interests: Diet, Brazil, Environmental Monitoring, Exposure Assessment, Child, and 15 moreFood Consumption, Female, Acetylcholinesterase, Fruit, Household Budget Survey, Cholinesterase inhibitors, Fruit and vegetables, Angiosperms, Adult, Food Contamination, Carbamates, General Population, Confidence Interval, Cumulant, and Child preschool
Intoxication due to pesticides in the Federal District of Brazil between 2004 and 2007 - analysis of notification to the Toxicological Information and Assistance Center | Intoxicação por agrotóxicos no Distrito Federal, Brasil, de 2004 a 2007 - análise da notificação ao Centro de Informação e Ass...more
A multiresidue method for the determination of 46 pesticides in fruits was validated. Samples were extracted with acidified ethyl acetate, MgSO4 and CH3COONa and cleaned up by dispersive SPE with PSA. The compounds were analysed by... more
A multiresidue method for the determination of 46 pesticides in fruits was validated. Samples were extracted with acidified ethyl acetate, MgSO4 and CH3COONa and cleaned up by dispersive SPE with PSA. The compounds were analysed by GC-FPD, GC-μECD or LC-MS/MS, with LOQs from 1 to 8 μg/kg. The method was used to analyse 238 kaki, cashew apple, guava, and peach fruit and pulp samples, which were also analysed for dithiocarbamates (DTCs) using a spectrophotometric method. Over 70% of the samples were positive, with DTC present in 46.5%, λ-cyhalothrin in 37.1%, and omethoate in 21.8% of the positive samples. GC-MS/MS confirmed the identities of the compounds detected by GC. None of the pesticides found in kaki, cashew apple and guava was authorised for these crops in Brazil. The risk assessment has shown that the cumulative acute intake of organophosphorus or pyrethroid compounds from the consumption of these fruits is unlikely to pose a health risk to consumers.
Research Interests: Brazil, Food Chemistry, Risk assessment, Pesticide Residues, Multidisciplinary, and 13 moreNUTS, Psidium, Fruit, Tandem Mass Spectrometry, Gas Chromatography, High Performance Liquid Chromatography, Food Additives, Malus, Prunus, Gas Chromatography/mass Spectrometry, Food Contamination, Risk Assessment, and Anacardium
The presence of mycotoxins in food has been associated with several human diseases, and health authorities have taken actions to decrease the ingestion of these compounds in the diet. A study was carried out to assess aflatoxins and... more
The presence of mycotoxins in food has been associated with several human diseases, and health authorities have taken actions to decrease the ingestion of these compounds in the diet. A study was carried out to assess aflatoxins and ochratoxin A concentrations found in food, and to evaluate the potential risk to human health resulting from mycotoxin exposure. Between July 1998 to December 2001, 366 food samples were analyzed, including peanuts and its products, nuts, maize, oat and/or wheat products, rice and beans. Samples were processed and the extracted mycotoxins were detected and separated using thin layer chromatography, and then quantified with fluorescence. Aflatoxins were detected in 19.6% of the samples: raw peanuts and its products, pop corn, maize and Brazilian nuts (>2mg/kg). Peanuts and its products showed the highest levels of aflatoxin contamination (34.7%) with up to 1280 mg/kg of AFB1+AFG1 and 1706 mg/kg of total aflatoxins. Of the positive samples, AFB1 was det...
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Breast milk samples collected from 18 nursing mothers between the 15th and 90th day of lactation were digested in nitric acid in a microwave, and total mercury (THg) levels were quantified by atomic fluorescence spectrometry. Participants... more
Breast milk samples collected from 18 nursing mothers between the 15th and 90th day of lactation were digested in nitric acid in a microwave, and total mercury (THg) levels were quantified by atomic fluorescence spectrometry. Participants responded to a 24-h dietary recall questionnaire on the 74th and 76th day of lactation and to a Food Frequency Questionnaire querying the frequency of fish intake over the last 90 days. Usual intake was estimated using the PC-SIDE software package. A meal of fish was offered on the 75th day of lactation. Mothers&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; individual mean THg levels ranged from &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.76 to 22.7 ng/mL during the period, and the mean level for all samples (n = 142) was 6.47 ±6.04 ng/mL. The multilevel mixed linear model used showed high heterogeneity of the mercury levels among the mothers, and THg levels did not change significantly over the period under study. However, a significant increase in THg levels was observed after the intervention with the fish meal. Exposure increased for most infants on the 90th day of lactation, with intakes exceeding the THg provisional tolerable weekly intake (PTWI) at least once during the period for 77.8 % of samples. Mothers consumed mostly food from the fat and grain groups, and a significant correlation was detected between consumption of food of these groups and breast milk THg levels (p = 0.006 and 0.007). A significant correlation was also found between vegetable consumption and carbohydrate intake and THg levels in the samples (p = 0.015 and 0.045, respectively). No correlation was found between mothers&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39; daily fish consumption frequency and THg levels. Although this study showed that mercury intake by infants during lactation may exceed the toxicologically safe exposure level (PTWI), we nevertheless believe that the benefits of lactation for both the mother and the infant outweigh the eventual risks that this exposure may represent.
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OBJETIVOS: A presença de micotoxinas em alimentos tem sido correlacionada a várias patologias humanas, e as autoridades de saúde no mundo todo têm implementado ações para diminuir a ingestão desses compostos pela dieta. Realizou-se... more
OBJETIVOS: A presença de micotoxinas em alimentos tem sido correlacionada a várias patologias humanas, e as autoridades de saúde no mundo todo têm implementado ações para diminuir a ingestão desses compostos pela dieta. Realizou-se pesquisa para analisar os níveis de aflatoxinas e ocratoxina A de alimentos para consumo e avaliar o potencial de risco da exposição humana a essas micotoxinas. MÉTODOS: Foram analisadas 366 amostras de alimentos consumidos no Distrito Federal, no período de julho de 1998 a dezembro de 2001, como amendoim e derivados, castanhas, milho, produtos de trigo e/ou aveia, arroz e feijão. As amostras foram processadas, e as micotoxinas extraídas, detectadas e quantificadas por fluorescência após separação em cromatografia camada delgada. RESULTADOS: Foram detectadas aflatoxinas em 19,6% das amostras, em amendoim cru e derivados, milho de pipoca, milho em grão e castanha-do-pará (>2 mig/kg). Amendoim e derivados apresentaram maior incidência de contaminação por...
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Research Interests:
OBJECTIVE: To assess the health risk of exposure to benzene for a community affected by a fuel leak. METHODS: Data regarding the fuel leak accident with, which occurred in the Brasilia, Federal District, were obtained from the Fuel... more
OBJECTIVE: To assess the health risk of exposure to benzene for a community affected by a fuel leak. METHODS: Data regarding the fuel leak accident with, which occurred in the Brasilia, Federal District, were obtained from the Fuel Distributor reports provided to the environmental authority. Information about the affected population (22 individuals) was obtained from focal groups of eight individuals. Length of exposure and water benzene concentration were estimated through a groundwater flow model associated with a benzene propagation model. The risk assessment was conducted according to the Agency for Toxic Substances and Disease Registry methodology. RESULTS: A high risk perception related to the health consequences of the accident was evident in the affected community (22 individuals), probably due to the lack of assistance and a poor risk communication from government authorities and the polluting agent. The community had been exposed to unsafe levels of benzene (> 5 µg/L) s...
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As prevalências das tentativas de suicídio provocadas pela exposição a agrotóxicos de uso agrícola no Estado do Mato Grosso do Sul, Brasil, ocorridas entre janeiro 1992 a dezembro 2002, foram avaliadas baseadas nos registros das... more
As prevalências das tentativas de suicídio provocadas pela exposição a agrotóxicos de uso agrícola no Estado do Mato Grosso do Sul, Brasil, ocorridas entre janeiro 1992 a dezembro 2002, foram avaliadas baseadas nos registros das notificações de intoxicação do Centro Integrado de Vigilância Toxicológica da Secretaria de Saúde do Estado. Dados populacionais e de produção agrícola foram obtidos do Instituto Brasileiro de Geografia e Estatística, e dados de suicídio por causas diversas da Secretaria de Estado de Saúde. Foram registradas 1.355 notificações de intoxicação, sendo 506 tentativas de suicídio que levaram a 139 óbitos. As microrregiões de Campo Grande e Dourados apresentam as maiores prevalências de tentativas de suicídio. Alta prevalência de suicídios por causas diversas também foi observada em Dourados, com uma tendência de crescimento nos últimos dez anos. Os resultados deste estudo indicaram a microrregião de Dourados como uma das mais críticas do Estado do Mato Grosso do ...
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Research Interests:
To evaluate the levels of antimony in plasma and skin of patients being treated with pentavalent antimonials (Glucantime) and their relationship with side effects. We evaluated 19 patients treated endovenously at the conventional dose (20... more
To evaluate the levels of antimony in plasma and skin of patients being treated with pentavalent antimonials (Glucantime) and their relationship with side effects. We evaluated 19 patients treated endovenously at the conventional dose (20 mg Sb(v)/kg/day), two at a smaller dose (5 mg Sb(v)/kg/day) and three treated intralesionally (up to 4.0 ml/week). During treatment, patients underwent periodic blood exams and were interviewed weekly about the incidence of adverse symptoms. The levels of antimony in plasma and skin samples were determined by Inductively Coupled Plasma with Mass Spectrometry (ICP-MS). The patients under conventional treatment presented a mean initial antimony plasma concentration of 3.39 microg/l; at the end of treatment, these levels were 0.21 before Glucantime application and 125.8 mg after Glucantime application. The mean antimony level in their skin at the end of the treatment was 9.24 microg/g. The main adverse symptoms were arthralgia and myalgia; laboratory results showed mainly lymphocytosis and eosinophilia. We found some significant correlations between antimony concentrations, adverse symptoms and laboratory alterations, strengthening the hypothesis of a dose-dependent relationship between antimony concentration in plasma and skin and side effects.